RchiOBHm_Chr1g0344351

ATP synthase subunit delta'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003919
Physical Location & Seq
Forward (+)
36662080 .. 36666726
4647 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGCTGCGCCGCGCCACCGCTCTCTTGACCCGACCCGCCTCCTCGCTCCGGGCCGCCCGAGCCTTCTCCACCGATGTCGCCGAAGCTCCCGTAGGCAACCCCTCTTTCAACGAGGCCTGGAAGAAGGTCATCCCTCACATCGATCCCCCCAAGACTCCTCTGTCCTTCATGAAGCCCCGCCCTCCGACCCCGTCCTCGATCCCTACCAAGCTCACCGTCAACTTCGTGCTGCCTTACGCATCTGAGCTCTCGGCCAAAGAGGTGGACATGGTCATAATTCCGGCAAGCACTGGGCAGATGGGTGTTCTTCCGGGACATGTGGCAACAATTGCAGAATTGAAACCTGGGGTCCTATCAGTACATGAAGGAAATGATGTGACAAAGTACTTCGTCAGCAGTGGTTTTGCATTCATCCATGCGAACTCTTATGCAGATATAATTGCTGTTGAGGCTGTCCCTATCGATCGAGTTGATCCAAGTCTTGTCCAGAAAGGGCTTGCGGAGTTCACTCAGAAGCTAAACTCAGCCTCAACTGACTTGGAGAAAGCTGAAGCCCAGATTGGAGTTGACGTGCATAGTGCCCTCAATGCTGCTCTCACAGGCTAG

Protein Analysis

201

Amino Acids

21.32

Weight (kDa)

6.51

Isoelectric Point (pI)

26.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_DE_N PF02823 71 - 142 5e-16 ATP synthase, Delta/Epsilon chain, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 160
AccBSI CCGCTC 1 cut(s) 20
AccII CGCG 1 cut(s) 12
AciI CCGC 6 cut(s) 10, 18, 36, 54, 178, 500
AclWI GGATC 3 cut(s) 137, 193, 467
AcoI YGGCCR 1 cut(s) 252
AcuI CTGAAG 1 cut(s) 570
AfaI GTAC 2 cut(s) 360, 386
AfiI CCNNNNNNNGG 1 cut(s) 48
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 2 cut(s) 109, 340
AjiI CACGTC 1 cut(s) 571
AjnI CCWGG 2 cut(s) 116, 343
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AluBI AGCT 5 cut(s) 86, 211, 247, 517, 548
AluI AGCT 5 cut(s) 86, 211, 247, 517, 548
Alw21I GWGCWC 1 cut(s) 249
AlwI GGATC 3 cut(s) 137, 193, 467
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 3 cut(s) 51, 114, 252
ApeKI GCWGC 3 cut(s) 4, 229, 590
AspLEI GCGC 2 cut(s) 9, 14
AspS9I GGNCC 2 cut(s) 51, 349
AsuC2I CCSGG 2 cut(s) 50, 312
AsuHPI GGTGA 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 216, 577
BccI CCATC 1 cut(s) 292
BciT130I CCWGG 2 cut(s) 118, 345
BcnI CCSGG 2 cut(s) 50, 312
BfaI CTAG 1 cut(s) 604
BisI GCNGC 5 cut(s) 5, 10, 54, 230, 591
BlsI GCNGC 5 cut(s) 6, 11, 55, 231, 592
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 4 cut(s) 50, 118, 312, 345
Bme18I GGWCC 1 cut(s) 349
BmeT110I CYCGRG 1 cut(s) 57
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 2 cut(s) 51, 349
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 4 cut(s) 50, 118, 312, 345
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 300
BpuMI CCSGG 2 cut(s) 50, 312
Bsa29I ATCGAT 2 cut(s) 141, 462
BsaJI CCNNGG 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 1 cut(s) 295
BseBI CCWGG 2 cut(s) 118, 345
BseCI ATCGAT 2 cut(s) 141, 462
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 129, 411
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMII CTCAG 3 cut(s) 234, 524, 537
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 2 cut(s) 31, 147
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 2 cut(s) 216, 577
Bsh1236I CGCG 1 cut(s) 12
Bsh1285I CGRYCG 1 cut(s) 466
BshFI GGCC 3 cut(s) 53, 116, 254
BshVI ATCGAT 2 cut(s) 141, 462
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 3 cut(s) 49, 281, 311
BslFI GGGAC 2 cut(s) 327, 440
BslI CCNNNNNNNGG 1 cut(s) 48
BsmFI GGGAC 2 cut(s) 327, 440
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 3 cut(s) 53, 116, 254
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 249, 583
Bsp143I GATC 4 cut(s) 142, 198, 463, 472
BspACI CCGC 6 cut(s) 10, 18, 36, 54, 178, 500
BspANI GGCC 3 cut(s) 53, 116, 254
BspCNI CTCAG 3 cut(s) 235, 523, 536
BspDI ATCGAT 2 cut(s) 141, 462
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 350
BspPI GGATC 3 cut(s) 137, 193, 467
BsrBI CCGCTC 1 cut(s) 20
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 4 cut(s) 142, 198, 463, 472
Bst2UI CCWGG 2 cut(s) 118, 345
Bst4CI ACNGT 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 286, 498
BstDEI CTNAG 3 cut(s) 243, 510, 523
BstF5I GGATG 2 cut(s) 129, 411
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 2 cut(s) 9, 14
BstKTI GATC 4 cut(s) 145, 201, 466, 475
BstMBI GATC 4 cut(s) 142, 198, 463, 472
BstMCI CGRYCG 1 cut(s) 466
BstMWI GCNNNNNNNGC 4 cut(s) 59, 329, 449, 587
BstNI CCWGG 2 cut(s) 118, 345
BstNSI RCATGY 1 cut(s) 320
BstSCI CCNGG 4 cut(s) 48, 116, 310, 343
BstSLI GKGCMC 1 cut(s) 583
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 216, 577
BstXI CCANNNNNNTGG 1 cut(s) 262
Bsu15I ATCGAT 2 cut(s) 141, 462
BsuRI GGCC 3 cut(s) 53, 116, 254
BsuTUI ATCGAT 2 cut(s) 141, 462
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 2 cut(s) 129, 411
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 2 cut(s) 288, 403
Cac8I GCNNGC 2 cut(s) 286, 498
CciI TCATGA 1 cut(s) 168
CfoI GCGC 2 cut(s) 9, 14
Cfr13I GGNCC 2 cut(s) 51, 349
ClaI ATCGAT 2 cut(s) 141, 462
Csp6I GTAC 2 cut(s) 359, 385
CviAII CATG 5 cut(s) 169, 268, 317, 362, 416
CviQI GTAC 2 cut(s) 359, 385
DdeI CTNAG 3 cut(s) 243, 510, 523
DpnI GATC 4 cut(s) 144, 200, 465, 474
DpnII GATC 4 cut(s) 142, 198, 463, 472
DrdI GACNNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 160
EaeI YGGCCR 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 570
Eco88I CYCGRG 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 2 cut(s) 116, 343
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 5 cut(s) 172, 271, 320, 365, 419
FaiI YATR 9 cut(s) 170, 269, 275, 318, 363, 417, 429, 437, 576
FaqI GGGAC 2 cut(s) 327, 440
FatI CATG 5 cut(s) 168, 267, 316, 361, 415
FauI CCCGC 2 cut(s) 43, 185
Fnu4HI GCNGC 5 cut(s) 5, 10, 54, 230, 591
FokI GGATG 2 cut(s) 116, 398
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 5 cut(s) 5, 10, 54, 230, 591
FspBI CTAG 1 cut(s) 604
GlaI GCGC 2 cut(s) 8, 13
GluI GCNGC 5 cut(s) 5, 10, 54, 230, 591
HaeIII GGCC 3 cut(s) 53, 116, 254
HapII CCGG 3 cut(s) 49, 281, 311
HhaI GCGC 2 cut(s) 9, 14
Hin1II CATG 5 cut(s) 172, 271, 320, 365, 419
Hin6I GCGC 2 cut(s) 7, 12
HinP1I GCGC 2 cut(s) 7, 12
HincII GTYRAC 2 cut(s) 220, 568
HindII GTYRAC 2 cut(s) 220, 568
HinfI GANTC 1 cut(s) 154
HpaII CCGG 3 cut(s) 49, 281, 311
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 4 cut(s) 220, 265, 507, 568
Hpy188I TCNGA 3 cut(s) 186, 244, 513
Hpy188III TCNNGA 3 cut(s) 25, 169, 487
Hpy8I GTNNAC 4 cut(s) 220, 265, 507, 568
HpyAV CCTTC 4 cut(s) 73, 118, 175, 359
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 4 cut(s) 332, 407, 431, 574
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 329, 449, 587
HpyF3I CTNAG 3 cut(s) 243, 510, 523
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 5 cut(s) 172, 271, 320, 365, 419
HspAI GCGC 2 cut(s) 7, 12
Kzo9I GATC 4 cut(s) 142, 198, 463, 472
LmnI GCTCC 2 cut(s) 51, 91
Lsp1109I GCAGC 2 cut(s) 216, 577
LweI GCATC 1 cut(s) 248
MaeI CTAG 1 cut(s) 604
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 1 cut(s) 376
MalI GATC 4 cut(s) 144, 200, 465, 474
MbiI CCGCTC 1 cut(s) 20
MboI GATC 4 cut(s) 142, 198, 463, 472
MboII GAAGA 2 cut(s) 133, 299
MfeI CAATTG 1 cut(s) 327
MhlI GDGCHC 2 cut(s) 249, 583
MluCI AATT 4 cut(s) 276, 327, 335, 438
MlyI GAGTC 1 cut(s) 148
MmeI TCCRAC 1 cut(s) 209
MspI CCGG 3 cut(s) 49, 281, 311
MspR9I CCNGG 4 cut(s) 50, 118, 312, 345
MteI GCGCNGCGC 1 cut(s) 10
MunI CAATTG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 2 cut(s) 118, 345
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 4 cut(s) 59, 329, 449, 587
NciI CCSGG 2 cut(s) 50, 312
NdeII GATC 4 cut(s) 142, 198, 463, 472
NlaIII CATG 5 cut(s) 172, 271, 320, 365, 419
NlaIV GGNNCC 1 cut(s) 350
NmeAIII GCCGAG 1 cut(s) 230
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 320
PagI TCATGA 1 cut(s) 168
PceI AGGCCT 1 cut(s) 116
PciI ACATGT 1 cut(s) 316
PctI GAATGC 1 cut(s) 407
PflFI GACNNNGTC 2 cut(s) 190, 269
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 5 cut(s) 6, 11, 55, 231, 592
Ple19I CGATCG 1 cut(s) 466
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PpuMI RGGWCCY 1 cut(s) 349
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 116, 343
PspGI CCWGG 2 cut(s) 116, 343
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 2 cut(s) 51, 349
PspPPI RGGWCCY 1 cut(s) 349
PsyI GACNNNGTC 2 cut(s) 190, 269
PvuI CGATCG 1 cut(s) 466
RsaI GTAC 2 cut(s) 360, 386
RsaNI GTAC 2 cut(s) 359, 385
SacI GAGCTC 1 cut(s) 249
SatI GCNGC 5 cut(s) 5, 10, 54, 230, 591
Sau3AI GATC 4 cut(s) 142, 198, 463, 472
Sau96I GGNCC 2 cut(s) 51, 349
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 1 cut(s) 148
ScrFI CCNGG 4 cut(s) 50, 118, 312, 345
SduI GDGCHC 2 cut(s) 249, 583
SetI ASST 9 cut(s) 88, 129, 213, 249, 264, 346, 519, 550, 573
SfaNI GCATC 1 cut(s) 248
SinI GGWCC 1 cut(s) 349
Sse9I AATT 4 cut(s) 276, 327, 335, 438
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 6 cut(s) 10, 18, 36, 54, 178, 500
SspMI CTAG 1 cut(s) 604
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 4 cut(s) 48, 116, 310, 343
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 1 cut(s) 573
TaqI TCGA 4 cut(s) 141, 197, 462, 466
TasI AATT 4 cut(s) 276, 327, 335, 438
TatI WGTACW 2 cut(s) 358, 384
TauI GCSGC 2 cut(s) 12, 56
TscAI CASTG 2 cut(s) 295, 403
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 3 cut(s) 4, 229, 590
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 4 cut(s) 157, 185, 378, 400
TspRI CASTG 2 cut(s) 295, 403
Tth111I GACNNNGTC 2 cut(s) 190, 269
VpaK11BI GGWCC 1 cut(s) 349
XceI RCATGY 1 cut(s) 320
XspI CTAG 1 cut(s) 604
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.