RchiOBHm_Chr1g0370371

AAA domain (Cdc48 subfamily)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007059
Physical Location & Seq
Forward (+)
59865752 .. 59870768
5017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3222 bp
ATGCCTACGCCGGTTTCCACGGCCAGGCAATGCTTAACTTCGGAGGCGGCCCACGCGCTCGACGAGGCGGTGTCCGTCGCGCGCCGGAGAGGCCACGCGCAGACCACTTCCCTCCACGCAATCTCCGCCCTCCTCTCTCTCCCCAACTCCGCCCTACGCGAGGCCTGCGCGCGCGCCCGCAACGCCGCCTACTCGCCGCGGCTCCAGTTCAAGGCGCTGGAGCTCTGCCTGAGCGTCTCGCTCGACCGCGTCTCCTCGACCCAGCAAAGCGACGACCCGCCCGTCTCGAACTCGCTCATGGCCGCCATCAAGCGGTCCCAGGCCAACCAGAGACGGCAGCCGGAGAACTACCACCTCTACCACCAGCTCCAGCAGCAGCAGCCGTCCATGTCCTGCGTTAAAGTGGAGCTCCAGCACCTGATTCTCTCTATTCTCGACGACCCGGTCGTCAGTCGGGTCTTCGGCGAAGCCGGTTTCCGGAGCTCCGAACTCAGACTCGCGGTTCTCCGCCCTTTTCCTCACCTCCTCAGATACTCCCGCTCCCAGGGGCATCCTCCTCCTCCTCCTCCCCTGTTTCTCTGCAATCCGGACCCGGGTCCGGGTCCGGGCCGTCGCCGGCCCGTTTTCCCCTTCTCGGGTTTCCCCAACGGAGATGAGAATTGTCGGAGAATTGGAGAAGTTCTAGGGAGGAATAGAAATCCCCTGCTTGTGGGTGTGTGTGCTTACGATGCGCTTCACATCTTCGTAGGCTCGTTAACTAAAGATGGGATTTTGCCGGTGGAGCTGTCCGGGCTGAGTTCAATCTCGATCGAAAAGGAGTTGTCCAAGTTCACAACGATCCATTGCGATAGAGGGTGCTTGAATTCGAGGCTGAGGGAGGTGGGGGAGTTGGCAGAGCAGTGTTTGGGACCTGGAATTGTTTTGAATATTGGAGACTTGAACATGTTTGTTGCTGAAGACTCTTTGGGTGACTCGGTGACCTATGTGGTTGCTCAGTTGACGAGGTTGGTGGAGCTTCACCGTGGAAGACTCTGGTTGATTGGCGCTACGGCTAGCTATGGGACCTACTTGAAGCTTGTGGGCATGTTTCCGACCGTCGAAAAAGACTGGGGCTTGCAGCTTCTCCCGATCACTTCGCTTGGAGCTGAATCATATCCAAGGTCCAGCTTGATGGAGTCATTTGTTCCATTTGGTGGGTTCTTTTCGGCACCTTCTGACCCAAAGCTTCCATTAAGCTGTTCAAATCGGTGCCTTCCCCGCAATCATCAGTGCAATGCTGTTCCAAAGGGAGGCTTTTCTGCTTCAGTGGCAGGTCAGCACCAAGCTAGCTTGCCTTCGTGGATGCAGATGGCTCCACTTGGCCCAAACAAGGGATCGGATATGAAGACCAAAGACAATGGAGTGCTATCAAGTTCCAAAGTTACAGTTTTGCAAGGGAAAGGGGATGATAATACTCATGAATCTCGCCCCCCGCCTTTAGCAAACTTGTTTCCAACTATAGTGGGCTTTGAGTCTGCTGAAGACAAGAAGCACAATCACAGCAACAAGACCAATATTTCTTCAAATGAAAATTCATGCATCCCCGTAGATGTGCAAGAGATATCCACATCTCAGTCCAGCGTTCCTCCGAAGGCTAAGAGTGAAAGTTTCTCCTCCGGAGTATGGGAAAAACCTACAAAAGATGAAGATATTGAATCGGGTAGTGTTAAGTCCTCTTACAGTTTGTCCAATTCAAGCATTGTTGAGGGTAGCCGAACTTCTCCAACATCTTCCGCTTCTGTGACAACAGATTTAGGATTAGGAATTTGCTCTTCTCCTGCTAGTAAGTTGAACTTAAATCTGGATGAAGGACGTCAACATGACATATCAGGTTTCTCGTCTTCTGATGTAGATTTATTCAATGGCAATTCCTCTATTTATACTGCTCAGTCATCATTCTGCTCAAGTCCTGACAAACATGGGCAGTTTGATCCAAGTGATGTCAAGATGCTTTTCAGAGCCCTCTTTGAGAGAGTGGGCTGGCAAACTGAAGCTATAAGTGCTATTAGCCAACGAATAGCTCGTTGCCACTCAAGAAGTGAACATCGGACTGGAGCTAGGCACAGAAGGGGAATGTGGTTCAACTTTGTTGGACCAGATAGGTATGGTAAAAAGAAAATTGCTAGTGTGGTTGCTGAGGTATTATATGGGAGCCAGAAACAATTGATCTGTGTGGATCTGAATTCTCAGGATGGGATGGTGCATTCAGACACAATGTTCGGTTGTCAAGTGTTGAATGGTTATGATGCAAAGTGTAGAGGTAAGACAGTTGTTGATTATGTTGCTGGAGAACTGTGCAAGAAACCATTGTCCGTTGTCCTCCTAGAAAATGTAGACAAGGCTGATGTTGTGGCTCAAAATAGTTTGTCCCAGGCTGTTTTGACCGGTAAATTTTCAGATTCCCATGGAAGACAAGTCAGCACAAGTAATGCAATGTTTATTACAACTACAGAAAAGGACTGCAGCATTCTCACTTCTAAAAAGGCACCTTCCAACTATTCCGAGGAAAGAATCTCACAAGCAAAAGGGTGGCCCTTGCAGATTACAGTTGAATGCGCGCTTGAAGGCATTACTGTAAGCCAAAACTGGAGGACATCCTCCAATACAACTAAAGAAAGCATCCCTCATTTTCTGAACAAAAGGAAGCTGTCTGGTACAGATGAACCTCTAGAGCTAGTCTCAGAGACAAGTAAACGGCCTAACAAAACGTCGACTAGGTATCTGGATCTCAACCTTCCAGCAGAAGAGAATGCAGTGCAACGCCTAGATGATGAGACCTCCGAAAATGACTGCTTCTCTGAAAACTCCAATTCCTGGTTGCAAGAATTCACCAATCAGGTGGATGAAACAGTGGTATTCAAGCCGGTTGATTTTGATGCACGTGCTGAGAAAATGTCAAAGGAGATCAAAAACAGTTTCCACAAGCTTGTTGGTTCAGACTGTTTGCTAGAAATCGACACACAGGTCGTAGAACAACTACTTGCAGCTGCATATTTGTCAGACAAGTACAGGGTGGTGGAAGATTGGGTGGAGCAAGTGTTGAGCAGAGGATTTGCAGAGGTTCAGAAGAGACATAACCTCAATGCTCGTACTACTGTAAAACTTAAACCTTGTGAGGGCATCTGCTTGGAGGAGGAGTCATCACCGGAAAATTACCTCCTACCTAAAATCATACTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1073

Amino Acids

117.76

Weight (kDa)

6.71

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 112 8.9e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 220 - 381 4.3e-16 SMAX1 nucleotide binding domain
AAA_2 PF07724 707 - 842 4e-11 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 970 - 1053 2e-30 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 281, 443, 446, 3002
AatII GACGTC 1 cut(s) 1855
Acc36I ACCTGC 1 cut(s) 1301
AccB1I GGYRCC 3 cut(s) 1207, 1248, 2524
AccB7I CCANNNNNTGG 2 cut(s) 1193, 2853
AccBSI CCGCTC 1 cut(s) 540
AccI GTMKAC 2 cut(s) 2373, 2750
AccIII TCCGGA 3 cut(s) 477, 586, 1655
AclWI GGATC 5 cut(s) 832, 1381, 1964, 2223, 2772
AcoI YGGCCR 2 cut(s) 21, 300
AcsI RAATTY 6 cut(s) 862, 1570, 1803, 2221, 2429, 2864
AcuI CTGAAG 4 cut(s) 975, 1287, 1539, 2049
AcvI CACGTG 1 cut(s) 2921
AcyI GRCGYC 1 cut(s) 1852
AfaI GTAC 3 cut(s) 2695, 3047, 3130
AflIII ACRYGT 1 cut(s) 942
AgeI ACCGGT 1 cut(s) 2423
AjnI CCWGG 6 cut(s) 23, 318, 543, 910, 2409, 2852
Alw21I GWGCWC 3 cut(s) 225, 411, 485
Alw26I GTCTC 9 cut(s) 241, 256, 289, 325, 927, 2717, 2722, 2807, 3103
AlwI GGATC 5 cut(s) 832, 1381, 1964, 2223, 2772
AlwNI CAGNNNCTG 1 cut(s) 418
Ama87I CYCGRG 2 cut(s) 592, 634
Aor13HI TCCGGA 3 cut(s) 477, 586, 1655
ApeKI GCWGC 8 cut(s) 337, 373, 376, 379, 1117, 2502, 3023, 3026
ApoI RAATTY 6 cut(s) 862, 1570, 1803, 2221, 2429, 2864
ArsI GACNNNNNNTTYG 2 cut(s) 2240, 2272
AsiGI ACCGGT 1 cut(s) 2423
AsuC2I CCSGG 6 cut(s) 443, 593, 594, 600, 606, 790
AsuHPI GGTGA 6 cut(s) 512, 980, 988, 1010, 2860, 3174
AsuNHI GCTAGC 2 cut(s) 1052, 1325
AvaI CYCGRG 2 cut(s) 592, 634
AvaII GGWCC 8 cut(s) 315, 589, 596, 602, 908, 1062, 1161, 2132
BanI GGYRCC 3 cut(s) 1207, 1248, 2524
BanII GRGCYC 4 cut(s) 225, 411, 485, 2002
BarI GAAGNNNNNNTAC 2 cut(s) 1741, 1773
BbrPI CACGTG 1 cut(s) 2921
BbsI GAAGAC 7 cut(s) 451, 963, 1033, 1391, 1527, 1872, 2455
Bbv12I GWGCWC 3 cut(s) 225, 411, 485
BbvCI CCTCAGC 2 cut(s) 872, 2175
BbvI GCAGC 8 cut(s) 349, 385, 388, 391, 1129, 2514, 3013, 3035
BccI CCATC 6 cut(s) 314, 758, 1165, 1342, 2225, 2230
BceAI ACGGC 6 cut(s) 36, 350, 367, 594, 1065, 2750
BciT130I CCWGG 6 cut(s) 25, 320, 545, 912, 2411, 2854
BcnI CCSGG 6 cut(s) 443, 593, 594, 600, 606, 790
BcoDI GTCTC 9 cut(s) 241, 256, 289, 325, 927, 2717, 2722, 2807, 3103
BfmI CTRYAG 3 cut(s) 1497, 2487, 2500
BfoI RGCGCY 2 cut(s) 218, 1047
BfuAI ACCTGC 1 cut(s) 1301
BglI GCCNNNNNGGC 1 cut(s) 90
Bme18I GGWCC 8 cut(s) 315, 589, 596, 602, 908, 1062, 1161, 2132
BmeT110I CYCGRG 2 cut(s) 592, 634
BmrI ACTGGG 1 cut(s) 1117
BmsI GCATC 9 cut(s) 559, 718, 1332, 1587, 1977, 2275, 2667, 2905, 3168
BmtI GCTAGC 2 cut(s) 1056, 1329
BmuI ACTGGG 1 cut(s) 1117
BoxI GACNNNNGTC 1 cut(s) 594
BpiI GAAGAC 7 cut(s) 451, 963, 1033, 1391, 1527, 1872, 2455
BplI GAGNNNNNCTC 2 cut(s) 2702, 2734
BpmI CTGGAG 7 cut(s) 188, 239, 353, 395, 2112, 2346, 2647
Bpu10I CCTNAGC 3 cut(s) 230, 872, 2175
BpuEI CTTGAG 2 cut(s) 1927, 2056
BpuMI CCSGG 6 cut(s) 443, 593, 594, 600, 606, 790
BsaAI YACGTR 1 cut(s) 2921
BsaHI GRCGYC 1 cut(s) 1852
BsaI GGTCTC 1 cut(s) 2807
BsaWI WCCGGW 5 cut(s) 477, 586, 1655, 2423, 3184
Bse118I RCCGGY 6 cut(s) 10, 470, 615, 775, 2423, 2902
Bse1I ACTGG 4 cut(s) 205, 1112, 2095, 2630
Bse3DI GCAATG 4 cut(s) 35, 841, 1279, 2478
BseAI TCCGGA 3 cut(s) 477, 586, 1655
BseBI CCWGG 6 cut(s) 25, 320, 545, 912, 2411, 2854
BseMI GCAATG 4 cut(s) 35, 841, 1279, 2478
BseNI ACTGG 4 cut(s) 205, 1112, 2095, 2630
BsePI GCGCGC 5 cut(s) 80, 168, 170, 172, 2595
BseXI GCAGC 8 cut(s) 349, 385, 388, 391, 1129, 2514, 3013, 3035
BseYI CCCAGC 1 cut(s) 261
Bsh1285I CGRYCG 4 cut(s) 247, 447, 810, 1095
BshNI GGYRCC 3 cut(s) 1207, 1248, 2524
BshTI ACCGGT 1 cut(s) 2423
BsiEI CGRYCG 4 cut(s) 247, 447, 810, 1095
BsiHKAI GWGCWC 3 cut(s) 225, 411, 485
BsiHKCI CYCGRG 2 cut(s) 592, 634
BslFI GGGAC 4 cut(s) 301, 921, 1075, 2392
BsmAI GTCTC 9 cut(s) 241, 256, 289, 325, 927, 2717, 2722, 2807, 3103
BsmBI CGTCTC 4 cut(s) 241, 256, 289, 325
BsmFI GGGAC 4 cut(s) 301, 921, 1075, 2392
BsmI GAATGC 4 cut(s) 2242, 2505, 2597, 2794
Bso31I GGTCTC 1 cut(s) 2807
BsoBI CYCGRG 2 cut(s) 592, 634
Bsp1286I GDGCHC 4 cut(s) 225, 411, 485, 2002
Bsp13I TCCGGA 3 cut(s) 477, 586, 1655
Bsp143I GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
Bsp19I CCATGG 1 cut(s) 2443
BspEI TCCGGA 3 cut(s) 477, 586, 1655
BspHI TCATGA 1 cut(s) 1456
BspMAI CTGCAG 1 cut(s) 2504
BspMI ACCTGC 1 cut(s) 1301
BspOI GCTAGC 2 cut(s) 1056, 1329
BspPI GGATC 5 cut(s) 832, 1381, 1964, 2223, 2772
BspQI GCTCTTC 1 cut(s) 1816
BspT107I GGYRCC 3 cut(s) 1207, 1248, 2524
BspTNI GGTCTC 1 cut(s) 2807
BsrBI CCGCTC 1 cut(s) 540
BsrDI GCAATG 4 cut(s) 35, 841, 1279, 2478
BsrFI RCCGGY 6 cut(s) 10, 470, 615, 775, 2423, 2902
BsrI ACTGG 4 cut(s) 205, 1112, 2095, 2630
BssAI RCCGGY 6 cut(s) 10, 470, 615, 775, 2423, 2902
BssHII GCGCGC 5 cut(s) 80, 168, 170, 172, 2595
BssMI GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
BssNI GRCGYC 1 cut(s) 1852
BssT1I CCWWGG 2 cut(s) 1157, 2443
Bst2UI CCWGG 6 cut(s) 25, 320, 545, 912, 2411, 2854
Bst6I CTCTTC 3 cut(s) 1816, 2778, 3101
BstACI GRCGYC 1 cut(s) 1852
BstBAI YACGTR 1 cut(s) 2921
BstDSI CCRYGG 4 cut(s) 18, 197, 1021, 2443
BstEII GGTNACC 1 cut(s) 976
BstENI CCTNNNNNAGG 1 cut(s) 158
BstH2I RGCGCY 2 cut(s) 218, 1047
BstKTI GATC 9 cut(s) 810, 840, 1131, 1376, 1972, 2208, 2218, 2767, 2946
BstMAI GTCTC 9 cut(s) 241, 256, 289, 325, 927, 2717, 2722, 2807, 3103
BstMBI GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
BstMCI CGRYCG 4 cut(s) 247, 447, 810, 1095
BstNI CCWGG 6 cut(s) 25, 320, 545, 912, 2411, 2854
BstNSI RCATGY 2 cut(s) 946, 1087
BstPAI GACNNNNGTC 1 cut(s) 594
BstPI GGTNACC 1 cut(s) 976
BstSFI CTRYAG 3 cut(s) 1497, 2487, 2500
BstV1I GCAGC 8 cut(s) 349, 385, 388, 391, 1129, 2514, 3013, 3035
BstV2I GAAGAC 7 cut(s) 451, 963, 1033, 1391, 1527, 1872, 2455
BstX2I RGATCY 2 cut(s) 2215, 2764
BstXI CCANNNNNNTGG 2 cut(s) 1171, 2878
BstYI RGATCY 2 cut(s) 2215, 2764
BtgI CCRYGG 4 cut(s) 18, 197, 1021, 2443
BtsI GCAGTG 2 cut(s) 905, 2799
BtsIMutI CAGTG 5 cut(s) 905, 1274, 1311, 2799, 2895
BveI ACCTGC 1 cut(s) 1301
CaiI CAGNNNCTG 1 cut(s) 418
CciI TCATGA 1 cut(s) 1456
Cfr10I RCCGGY 6 cut(s) 10, 470, 615, 775, 2423, 2902
Cfr42I CCGCGG 1 cut(s) 200
Cfr9I CCCGGG 1 cut(s) 592
CseI GACGC 2 cut(s) 223, 238
Csp6I GTAC 3 cut(s) 2694, 3046, 3129
CspAI ACCGGT 1 cut(s) 2423
CspCI CAANNNNNGTGG 2 cut(s) 1482, 1517
CviAII CATG 9 cut(s) 298, 388, 943, 1084, 1457, 1575, 1859, 1958, 2444
CviQI GTAC 3 cut(s) 2694, 3046, 3129
DpnI GATC 9 cut(s) 809, 839, 1130, 1375, 1971, 2207, 2217, 2766, 2945
DpnII GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
DrdI GACNNNNNNGTC 4 cut(s) 281, 443, 446, 3002
DseDI GACNNNNNNGTC 4 cut(s) 281, 443, 446, 3002
EaeI YGGCCR 2 cut(s) 21, 300
Eam1104I CTCTTC 3 cut(s) 1816, 2778, 3101
EarI CTCTTC 3 cut(s) 1816, 2778, 3101
EciI GGCGGA 3 cut(s) 115, 139, 497
Ecl136II GAGCTC 3 cut(s) 223, 409, 483
Eco130I CCWWGG 2 cut(s) 1157, 2443
Eco147I AGGCCT 1 cut(s) 164
Eco24I GRGCYC 4 cut(s) 225, 411, 485, 2002
Eco31I GGTCTC 1 cut(s) 2807
Eco32I GATATC 1 cut(s) 1602
Eco47I GGWCC 8 cut(s) 315, 589, 596, 602, 908, 1062, 1161, 2132
Eco53kI GAGCTC 3 cut(s) 223, 409, 483
Eco57I CTGAAG 4 cut(s) 975, 1287, 1539, 2049
Eco72I CACGTG 1 cut(s) 2921
Eco88I CYCGRG 2 cut(s) 592, 634
Eco91I GGTNACC 1 cut(s) 976
EcoICRI GAGCTC 3 cut(s) 223, 409, 483
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 2 cut(s) 908, 1062
EcoO65I GGTNACC 1 cut(s) 976
EcoRI GAATTC 3 cut(s) 862, 2221, 2864
EcoRII CCWGG 6 cut(s) 23, 318, 543, 910, 2409, 2852
EcoRV GATATC 1 cut(s) 1602
EcoT14I CCWWGG 2 cut(s) 1157, 2443
EcoT22I ATGCAT 1 cut(s) 1580
EcoT38I GRGCYC 4 cut(s) 225, 411, 485, 2002
ErhI CCWWGG 2 cut(s) 1157, 2443
Esp3I CGTCTC 4 cut(s) 241, 256, 289, 325
FaeI CATG 9 cut(s) 301, 391, 946, 1087, 1460, 1578, 1862, 1961, 2447
FalI AAGNNNNNCTT 4 cut(s) 1277, 1309, 1817, 1849
FaqI GGGAC 4 cut(s) 301, 921, 1075, 2392
FatI CATG 9 cut(s) 297, 387, 942, 1083, 1456, 1574, 1858, 1957, 2443
FauI CCCGC 5 cut(s) 185, 285, 545, 1265, 1479
FblI GTMKAC 2 cut(s) 2373, 2750
FriOI GRGCYC 4 cut(s) 225, 411, 485, 2002
GsaI CCCAGC 1 cut(s) 265
GsuI CTGGAG 7 cut(s) 188, 239, 353, 395, 2112, 2346, 2647
HaeII RGCGCY 2 cut(s) 218, 1047
HgaI GACGC 2 cut(s) 223, 238
Hin1I GRCGYC 1 cut(s) 1852
Hin1II CATG 9 cut(s) 301, 391, 946, 1087, 1460, 1578, 1862, 1961, 2447
HincII GTYRAC 4 cut(s) 756, 999, 1856, 2751
HindII GTYRAC 4 cut(s) 756, 999, 1856, 2751
HindIII AAGCTT 3 cut(s) 1073, 1223, 2963
HpaI GTTAAC 1 cut(s) 756
HphI GGTGA 6 cut(s) 512, 980, 988, 1010, 2860, 3174
Hpy166II GTNNAC 8 cut(s) 756, 831, 999, 1856, 2081, 2374, 2732, 2751
Hpy8I GTNNAC 8 cut(s) 756, 831, 999, 1856, 2081, 2374, 2732, 2751
Hpy99I CGWCG 7 cut(s) 65, 80, 275, 440, 615, 1100, 2752
HpyCH4IV ACGT 3 cut(s) 1852, 2747, 2920
HpySE526I ACGT 3 cut(s) 1852, 2747, 2920
Hsp92I GRCGYC 1 cut(s) 1852
Hsp92II CATG 9 cut(s) 301, 391, 946, 1087, 1460, 1578, 1862, 1961, 2447
Kpn2I TCCGGA 3 cut(s) 477, 586, 1655
KroI GCCGGC 1 cut(s) 615
KroNI GCCGGC 1 cut(s) 617
KspAI GTTAAC 1 cut(s) 756
KspI CCGCGG 1 cut(s) 200
Kzo9I GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
LguI GCTCTTC 1 cut(s) 1816
Lsp1109I GCAGC 8 cut(s) 349, 385, 388, 391, 1129, 2514, 3013, 3035
LweI GCATC 9 cut(s) 559, 718, 1332, 1587, 1977, 2275, 2667, 2905, 3168
MaeII ACGT 3 cut(s) 1852, 2747, 2920
MaeIII GTNAC 4 cut(s) 968, 976, 1420, 1780
MalI GATC 9 cut(s) 809, 839, 1130, 1375, 1971, 2207, 2217, 2766, 2945
MauBI CGCGCGCG 1 cut(s) 170
MbiI CCGCTC 1 cut(s) 540
MboI GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
MfeI CAATTG 1 cut(s) 2201
MflI RGATCY 2 cut(s) 2215, 2764
MhlI GDGCHC 4 cut(s) 225, 411, 485, 2002
MlyI GAGTC 7 cut(s) 489, 953, 965, 1023, 1184, 1520, 3185
MmeI TCCRAC 6 cut(s) 644, 1115, 1517, 1787, 2110, 2556
Mph1103I ATGCAT 1 cut(s) 1580
MroI TCCGGA 3 cut(s) 477, 586, 1655
MroNI GCCGGC 1 cut(s) 615
MseI TTAA 7 cut(s) 35, 399, 755, 1232, 1707, 1835, 3144
MspA1I CMGCKG 2 cut(s) 199, 3026
MunI CAATTG 1 cut(s) 2201
Mva1269I GAATGC 4 cut(s) 2242, 2505, 2597, 2794
MvaI CCWGG 6 cut(s) 25, 320, 545, 912, 2411, 2854
NaeI GCCGGC 1 cut(s) 617
NciI CCSGG 6 cut(s) 443, 593, 594, 600, 606, 790
NcoI CCATGG 1 cut(s) 2443
NdeII GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
NgoMIV GCCGGC 1 cut(s) 615
NheI GCTAGC 2 cut(s) 1052, 1325
NlaIII CATG 9 cut(s) 301, 391, 946, 1087, 1460, 1578, 1862, 1961, 2447
NmuCI GTSAC 3 cut(s) 968, 976, 1780
NsiI ATGCAT 1 cut(s) 1580
NspI RCATGY 2 cut(s) 946, 1087
PagI TCATGA 1 cut(s) 1456
PasI CCCWGGG 1 cut(s) 544
PauI GCGCGC 5 cut(s) 80, 168, 170, 172, 2595
PceI AGGCCT 1 cut(s) 164
PciI ACATGT 1 cut(s) 942
PciSI GCTCTTC 1 cut(s) 1816
PcsI WCGNNNNNNNCGW 4 cut(s) 60, 279, 444, 2059
PctI GAATGC 4 cut(s) 2242, 2505, 2597, 2794
PdiI GCCGGC 1 cut(s) 617
PfeI GAWTC 6 cut(s) 421, 1148, 1460, 1694, 2438, 2550
PflFI GACNNNGTC 2 cut(s) 248, 443
PflMI CCANNNNNTGG 2 cut(s) 1193, 2853
PinAI ACCGGT 1 cut(s) 2423
Ple19I CGATCG 1 cut(s) 810
PleI GAGTC 7 cut(s) 489, 953, 965, 1023, 1183, 1519, 3184
PmaCI CACGTG 1 cut(s) 2921
PmlI CACGTG 1 cut(s) 2921
PpsI GAGTC 7 cut(s) 489, 953, 965, 1023, 1183, 1519, 3184
Ppu21I YACGTR 1 cut(s) 2921
PpuMI RGGWCCY 2 cut(s) 908, 1062
PscI ACATGT 1 cut(s) 942
PshAI GACNNNNGTC 1 cut(s) 594
Psp124BI GAGCTC 3 cut(s) 225, 411, 485
Psp5II RGGWCCY 2 cut(s) 908, 1062
Psp6I CCWGG 6 cut(s) 23, 318, 543, 910, 2409, 2852
PspCI CACGTG 1 cut(s) 2921
PspEI GGTNACC 1 cut(s) 976
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 6 cut(s) 23, 318, 543, 910, 2409, 2852
PspPPI RGGWCCY 2 cut(s) 908, 1062
PstI CTGCAG 1 cut(s) 2504
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 2 cut(s) 2215, 2764
PsyI GACNNNGTC 2 cut(s) 248, 443
PteI GCGCGC 5 cut(s) 80, 168, 170, 172, 2595
PvuI CGATCG 1 cut(s) 810
PvuII CAGCTG 1 cut(s) 3026
RsaI GTAC 3 cut(s) 2695, 3047, 3130
RsaNI GTAC 3 cut(s) 2694, 3046, 3129
SacI GAGCTC 3 cut(s) 225, 411, 485
SacII CCGCGG 1 cut(s) 200
SalI GTCGAC 1 cut(s) 2749
SapI GCTCTTC 1 cut(s) 1816
SaqAI TTAA 7 cut(s) 35, 399, 755, 1232, 1707, 1835, 3144
Sau3AI GATC 9 cut(s) 807, 837, 1128, 1373, 1969, 2205, 2215, 2764, 2943
SchI GAGTC 7 cut(s) 489, 953, 965, 1023, 1184, 1520, 3185
SduI GDGCHC 4 cut(s) 225, 411, 485, 2002
SfaNI GCATC 9 cut(s) 559, 718, 1332, 1587, 1977, 2275, 2667, 2905, 3168
SfcI CTRYAG 3 cut(s) 1497, 2487, 2500
Sfr303I CCGCGG 1 cut(s) 200
SgrBI CCGCGG 1 cut(s) 200
SinI GGWCC 8 cut(s) 315, 589, 596, 602, 908, 1062, 1161, 2132
SmaI CCCGGG 1 cut(s) 594
SmlI CTYRAG 2 cut(s) 1942, 2071
SmoI CTYRAG 2 cut(s) 1942, 2071
SseBI AGGCCT 1 cut(s) 164
SspI AATATT 2 cut(s) 928, 1555
SstI GAGCTC 3 cut(s) 225, 411, 485
StuI AGGCCT 1 cut(s) 164
StyI CCWWGG 2 cut(s) 1157, 2443
TaiI ACGT 3 cut(s) 1855, 2750, 2923
TatI WGTACW 1 cut(s) 3045
TauI GCSGC 5 cut(s) 50, 188, 199, 202, 305
TfiI GAWTC 6 cut(s) 421, 1148, 1460, 1694, 2438, 2550
Tru1I TTAA 7 cut(s) 35, 399, 755, 1232, 1707, 1835, 3144
Tru9I TTAA 7 cut(s) 35, 399, 755, 1232, 1707, 1835, 3144
TscAI CASTG 5 cut(s) 905, 1274, 1311, 2799, 2895
TseFI GTSAC 3 cut(s) 968, 976, 1780
TseI GCWGC 8 cut(s) 337, 373, 376, 379, 1117, 2502, 3023, 3026
Tsp45I GTSAC 3 cut(s) 968, 976, 1780
TspDTI ATGAA 8 cut(s) 1397, 1473, 1563, 1581, 1698, 1860, 2715, 2898
TspGWI ACGGA 3 cut(s) 64, 663, 2341
TspMI CCCGGG 1 cut(s) 592
TspRI CASTG 5 cut(s) 905, 1274, 1311, 2799, 2895
Tth111I GACNNNGTC 2 cut(s) 248, 443
Van91I CCANNNNNTGG 2 cut(s) 1193, 2853
VpaK11BI GGWCC 8 cut(s) 315, 589, 596, 602, 908, 1062, 1161, 2132
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 6 cut(s) 862, 1570, 1803, 2221, 2429, 2864
XbaI TCTAGA 1 cut(s) 2707
XceI RCATGY 2 cut(s) 946, 1087
XcmI CCANNNNNNNNNTGG 1 cut(s) 2966
XmaI CCCGGG 1 cut(s) 592
XmiI GTMKAC 2 cut(s) 2373, 2750
ZraI GACGTC 1 cut(s) 1853
Zsp2I ATGCAT 1 cut(s) 1580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.