RchiOBHm_Chr1g0374111

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010964
Physical Location & Seq
Reverse (-)
62041749 .. 62042992
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGCAAAAGAAGGGGCAAGAAAATAGAGAAGGTAAAAATAATAGTCAGAATGCTACTTTTGCTATCCCTAGGTTTTTGCTGCAGCGCGGGAATACTAGCCCTAGTCCGAGTCCACGCCCTAGCTTTGGCTCGACGAAGGGGAGGAAGTCGCCGGTCTCTCCTTCGGCTTGGGCGTTGTCTCCGGGACGGTCTTTGCCATGCACGGTGGGACCGGAGCTGACTGCGGCGGCAGTGGCTTCGCCGAGACCGAGGGTGAAGTCTAGTGGGGGTGGAGTTGGTGGGGTTTTGAAGTATTTTAGGCTTCCGAAGAAAGGGATGACTATACAAGAGGAGGAGTTTCATCAGTTTCGGATTTTGCATAATAGGTTGCTCCAATGGAGATTTGACAATGCTCGAGCTCAAGCTGCCATGGTTGCTTCACACAAAGTTGCAAAGGACAGAATGTTCAGTGTGTGGCTTAGAATTTTCAAAATGAGGAACTACATCCTAGATAAGCAACTACAACTGCAAAAGCTCAAGCATCAAGTGAAGGTGTATCAAATTGTGTACCCTCAAATTTCTCTACTGAACGAATGGGGGAAATTGGAGAGAAAAAATCAAGAATCTGTGAGCAGGATGGTGAGGAAGTTATCCGGAATTTCAAATATAATTCCTTTGGTAAATGATGCTAAGGGAGATGTGGCGTCTATATACGAAGCGATGATCACGGCCATGGATGTCATGGCTGGTATTCAAGCAACGGTCACTAAATTGCTCCCGCAGCTCGAAAAGCTGCTCTACATGGTTACGGAGCTTTTGATCATGCAGAAGCAACAAAGAGAATTAGAGAAATATATTACATCGGTCGTTGGTCTTATGGTAAGTGATATTTGCCGTTGCTGTCAAGCTCCTAAATGTATTGTACATTCATATCATTTTGGTAGAATCTGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

311

Amino Acids

35.06

Weight (kDa)

10.14

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 24 - 275 4.9e-67 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 632
AciI CCGC 4 cut(s) 87, 224, 227, 758
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 3 cut(s) 462, 555, 636
AcyI GRCGYC 1 cut(s) 683
AfaI GTAC 2 cut(s) 548, 903
AfiI CCNNNNNNNGG 2 cut(s) 125, 311
AgsI TTSAA 4 cut(s) 289, 469, 642, 734
AloI GAACNNNNNNTCC 2 cut(s) 428, 460
AluBI AGCT 9 cut(s) 123, 217, 398, 404, 514, 763, 772, 793, 887
AluI AGCT 9 cut(s) 123, 217, 398, 404, 514, 763, 772, 793, 887
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 3 cut(s) 160, 183, 238
Ama87I CYCGRG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 632
AoxI GGCC 1 cut(s) 708
ApeKI GCWGC 5 cut(s) 79, 82, 404, 760, 772
ApoI RAATTY 3 cut(s) 462, 555, 636
AspA2I CCTAGG 1 cut(s) 68
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 2 cut(s) 265, 631
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 1 cut(s) 209
AvrII CCTAGG 1 cut(s) 68
BanII GRGCYC 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 5 cut(s) 66, 94, 391, 759, 772
BccI CCATC 1 cut(s) 610
BceAI ACGGC 2 cut(s) 723, 858
BcgI CGANNNNNNTGC 2 cut(s) 219, 253
BclI TGATCA 2 cut(s) 702, 798
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 3 cut(s) 160, 183, 238
BfaI CTAG 6 cut(s) 69, 96, 102, 120, 261, 488
BfmI CTRYAG 1 cut(s) 80
BisI GCNGC 7 cut(s) 80, 83, 225, 228, 405, 761, 773
BlnI CCTAGG 1 cut(s) 68
BlsI GCNGC 7 cut(s) 81, 84, 226, 229, 406, 762, 774
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 393
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 210
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 529, 655
Bpu10I CCTNAGC 1 cut(s) 669
BpuEI CTTGAG 2 cut(s) 384, 500
BpuMI CCSGG 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 683
BsaI GGTCTC 2 cut(s) 160, 238
BsaJI CCNNGG 4 cut(s) 68, 248, 408, 711
BsaWI WCCGGW 2 cut(s) 211, 632
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 311
Bse118I RCCGGY 1 cut(s) 151
BseAI TCCGGA 1 cut(s) 632
BseDI CCNNGG 4 cut(s) 68, 248, 408, 711
BseGI GGATG 4 cut(s) 321, 483, 621, 721
BseLI CCNNNNNNNGG 2 cut(s) 125, 311
BseRI GAGGAG 2 cut(s) 344, 347
BseXI GCAGC 5 cut(s) 66, 94, 391, 759, 772
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 846
BshFI GGCC 1 cut(s) 710
BsiEI CGRYCG 1 cut(s) 846
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 4 cut(s) 152, 182, 212, 633
BslFI GGGAC 2 cut(s) 198, 222
BslI CCNNNNNNNGG 2 cut(s) 125, 311
BsmAI GTCTC 3 cut(s) 160, 183, 238
BsmFI GGGAC 2 cut(s) 198, 222
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 1 cut(s) 710
Bso31I GGTCTC 2 cut(s) 160, 238
BsoBI CYCGRG 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 400
Bsp13I TCCGGA 1 cut(s) 632
Bsp1407I TGTACA 1 cut(s) 901
Bsp143I GATC 2 cut(s) 702, 798
Bsp19I CCATGG 2 cut(s) 408, 711
BspACI CCGC 4 cut(s) 87, 224, 227, 758
BspANI GGCC 1 cut(s) 710
BspEI TCCGGA 1 cut(s) 632
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 210
BspMAI CTGCAG 1 cut(s) 84
BspTNI GGTCTC 2 cut(s) 160, 238
BsrFI RCCGGY 1 cut(s) 151
BsrGI TGTACA 1 cut(s) 901
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 4 cut(s) 68, 248, 408, 711
BssMI GATC 2 cut(s) 702, 798
BssNI GRCGYC 1 cut(s) 683
BssT1I CCWWGG 3 cut(s) 68, 408, 711
Bst4CI ACNGT 3 cut(s) 189, 205, 742
BstACI GRCGYC 1 cut(s) 683
BstAUI TGTACA 1 cut(s) 901
BstDEI CTNAG 2 cut(s) 458, 669
BstDSI CCRYGG 2 cut(s) 408, 711
BstF5I GGATG 4 cut(s) 321, 483, 621, 721
BstFNI CGCG 1 cut(s) 87
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 2 cut(s) 705, 801
BstMAI GTCTC 3 cut(s) 160, 183, 238
BstMBI GATC 2 cut(s) 702, 798
BstMCI CGRYCG 1 cut(s) 846
BstMWI GCNNNNNNNGC 6 cut(s) 59, 233, 404, 413, 760, 769
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 5 cut(s) 66, 94, 391, 759, 772
BsuRI GGCC 1 cut(s) 710
BtgI CCRYGG 2 cut(s) 408, 711
BtgZI GCGATG 1 cut(s) 713
BtsCI GGATG 4 cut(s) 321, 483, 621, 721
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 237, 454
CfoI GCGC 1 cut(s) 87
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 1 cut(s) 672
Csp6I GTAC 2 cut(s) 547, 902
CviAII CATG 6 cut(s) 198, 409, 712, 721, 781, 802
CviQI GTAC 2 cut(s) 547, 902
DdeI CTNAG 2 cut(s) 458, 669
DpnI GATC 2 cut(s) 704, 800
DpnII GATC 2 cut(s) 702, 798
EaeI YGGCCR 1 cut(s) 708
Ecl136II GAGCTC 1 cut(s) 398
Eco130I CCWWGG 3 cut(s) 68, 408, 711
Eco24I GRGCYC 1 cut(s) 400
Eco31I GGTCTC 2 cut(s) 160, 238
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 398
Eco88I CYCGRG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 398
EcoT14I CCWWGG 3 cut(s) 68, 408, 711
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 3 cut(s) 68, 408, 711
FaeI CATG 6 cut(s) 201, 412, 715, 724, 784, 805
FaqI GGGAC 2 cut(s) 198, 222
FatI CATG 6 cut(s) 197, 408, 711, 720, 780, 801
FauI CCCGC 2 cut(s) 80, 765
FbaI TGATCA 2 cut(s) 702, 798
Fnu4HI GCNGC 7 cut(s) 80, 83, 225, 228, 405, 761, 773
FokI GGATG 4 cut(s) 328, 470, 628, 728
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 7 cut(s) 80, 83, 225, 228, 405, 761, 773
FspBI CTAG 6 cut(s) 69, 96, 102, 120, 261, 488
GlaI GCGC 1 cut(s) 86
GluI GCNGC 7 cut(s) 80, 83, 225, 228, 405, 761, 773
HaeIII GGCC 1 cut(s) 710
HapII CCGG 4 cut(s) 152, 182, 212, 633
HgaI GACGC 1 cut(s) 672
HhaI GCGC 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 683
Hin1II CATG 6 cut(s) 201, 412, 715, 724, 784, 805
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HinfI GANTC 3 cut(s) 109, 602, 924
HpaII CCGG 4 cut(s) 152, 182, 212, 633
HphI GGTGA 2 cut(s) 265, 631
Hpy166II GTNNAC 2 cut(s) 113, 547
Hpy188I TCNGA 4 cut(s) 48, 108, 306, 351
Hpy188III TCNNGA 2 cut(s) 599, 633
Hpy8I GTNNAC 2 cut(s) 113, 547
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 5 cut(s) 4, 23, 130, 171, 523
HpyCH4III ACNGT 3 cut(s) 189, 205, 742
HpyCH4V TGCA 7 cut(s) 4, 82, 201, 358, 431, 508, 805
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 233, 404, 413, 760, 769
HpyF3I CTNAG 2 cut(s) 458, 669
Hsp92I GRCGYC 1 cut(s) 683
Hsp92II CATG 6 cut(s) 201, 412, 715, 724, 784, 805
HspAI GCGC 1 cut(s) 85
Kpn2I TCCGGA 1 cut(s) 632
Ksp22I TGATCA 2 cut(s) 702, 798
Kzo9I GATC 2 cut(s) 702, 798
LmnI GCTCC 5 cut(s) 214, 375, 759, 790, 892
LpnPI CCDG 6 cut(s) 165, 195, 225, 598, 646, 711
Lsp1109I GCAGC 5 cut(s) 66, 94, 391, 759, 772
LweI GCATC 2 cut(s) 529, 655
MaeI CTAG 6 cut(s) 69, 96, 102, 120, 261, 488
MaeIII GTNAC 2 cut(s) 742, 784
MalI GATC 2 cut(s) 704, 800
MboI GATC 2 cut(s) 702, 798
MboII GAAGA 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 8 cut(s) 462, 540, 555, 581, 636, 648, 749, 821
MlyI GAGTC 1 cut(s) 118
MnlI CCTC 7 cut(s) 135, 243, 322, 325, 468, 561, 615
MroI TCCGGA 1 cut(s) 632
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 4 cut(s) 152, 182, 212, 633
MspR9I CCNGG 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 55
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 6 cut(s) 59, 233, 404, 413, 760, 769
NciI CCSGG 1 cut(s) 183
NcoI CCATGG 2 cut(s) 408, 711
NdeII GATC 2 cut(s) 702, 798
NlaIII CATG 6 cut(s) 201, 412, 715, 724, 784, 805
NlaIV GGNNCC 1 cut(s) 210
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 742
PaeR7I CTCGAG 1 cut(s) 393
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 245
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 2 cut(s) 602, 924
PfoI TCCNGGA 1 cut(s) 181
PkrI GCNGC 7 cut(s) 81, 84, 226, 229, 406, 762, 774
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 1 cut(s) 209
PspXI VCTCGAGB 1 cut(s) 393
PstI CTGCAG 1 cut(s) 84
RsaI GTAC 2 cut(s) 548, 903
RsaNI GTAC 2 cut(s) 547, 902
RseI CAYNNNNRTG 1 cut(s) 710
SacI GAGCTC 1 cut(s) 400
SatI GCNGC 7 cut(s) 80, 83, 225, 228, 405, 761, 773
Sau3AI GATC 2 cut(s) 702, 798
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 2 cut(s) 529, 655
SfcI CTRYAG 1 cut(s) 80
Sfr274I CTCGAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 3 cut(s) 393, 399, 515
SmoI CTYRAG 3 cut(s) 393, 399, 515
Sse9I AATT 8 cut(s) 462, 540, 555, 581, 636, 648, 749, 821
SsiI CCGC 4 cut(s) 87, 224, 227, 758
SspMI CTAG 6 cut(s) 69, 96, 102, 120, 261, 488
SstI GAGCTC 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 3 cut(s) 68, 408, 711
TaaI ACNGT 3 cut(s) 189, 205, 742
TaqI TCGA 3 cut(s) 131, 394, 765
TaqII GACCGA 2 cut(s) 262, 832
TasI AATT 8 cut(s) 462, 540, 555, 581, 636, 648, 749, 821
TatI WGTACW 1 cut(s) 901
TauI GCSGC 2 cut(s) 227, 230
TfiI GAWTC 2 cut(s) 602, 924
TscAI CASTG 2 cut(s) 237, 454
TseFI GTSAC 1 cut(s) 742
TseI GCWGC 5 cut(s) 79, 82, 404, 760, 772
Tsp45I GTSAC 1 cut(s) 742
TspDTI ATGAA 2 cut(s) 329, 897
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 2 cut(s) 237, 454
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 3 cut(s) 462, 555, 636
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XhoI CTCGAG 1 cut(s) 393
XmaJI CCTAGG 1 cut(s) 68
XspI CTAG 6 cut(s) 69, 96, 102, 120, 261, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.