RchiOBHm_Chr0c38g0503091

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0007486
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGGAGAGTCATATAAGAAGCGTAATGAACAGTTGAAATCATTCAAGGATGACCCAGTTGTGGAATCCCTTGCCAACAACGTAAGAATACGTGCTGAGGCTGTCAACAACATACGTAAGAAGGAGGGTTTTTCTATCGCTTTCCTGAAAGAAGTGACGGCATTTGTTTCCTACATGAATGGCCAAGCTGCCTCTGTAATCTTGGCATGCAAACAGGATATATCGACTAGCAACAAGAGCTTTAAGCTGGTTGTCAATGAGTACTTGAACAATAGTGAGCAGCTGCTGGACTTCTGTTCTGCATTGGATAAGTTCGTGAAATCAGCTCGGGATAGTCATTTATATACACAATATGTGGTTCAACAGTGCTTTGACAACAAGATGGAAACTGAGTACTGCCTGAAGATTTTGGAGAAACTGAAGATTCTCAAGTCCTCTGATGATATCCATACTGATTCTGCTGAGAGCTTGCACCAAAAGATCAAAGGTTTGATTGCGCAGTTTGAAAAACTATGCAAGAATTTGGTGCACGAAATTTTAAGATTAATGAAGAGAAGAATCGTGTTGGTACTCAGAAGAAACACACAAGTCTTGCATATAAGAGTGTTTCTACGATTGGTTTGGTATTTGCAGGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

213

Amino Acids

24.78

Weight (kDa)

9.29

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 41 - 188 1.9e-13 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 498
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 2 cut(s) 520, 534
AcuI CTGAAG 2 cut(s) 422, 440
AfaI GTAC 3 cut(s) 263, 395, 570
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 5 cut(s) 37, 46, 268, 362, 506
AluBI AGCT 6 cut(s) 188, 240, 247, 283, 326, 468
AluI AGCT 6 cut(s) 188, 240, 247, 283, 326, 468
Alw21I GWGCWC 1 cut(s) 531
Alw44I GTGCAC 1 cut(s) 527
AlwNI CAGNNNCTG 1 cut(s) 286
Ama87I CYCGRG 1 cut(s) 327
AoxI GGCC 1 cut(s) 181
ApaLI GTGCAC 1 cut(s) 527
ApeKI GCWGC 3 cut(s) 188, 280, 283
ApoI RAATTY 2 cut(s) 520, 534
AseI ATTAAT 1 cut(s) 545
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 499
AvaI CYCGRG 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 531
BalI TGGCCA 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 531
BbvCI CCTCAGC 1 cut(s) 96
BbvI GCAGC 3 cut(s) 175, 270, 292
BccI CCATC 1 cut(s) 376
BceAI ACGGC 1 cut(s) 174
BfaI CTAG 1 cut(s) 228
BisI GCNGC 3 cut(s) 189, 281, 284
BlsI GCNGC 3 cut(s) 190, 282, 285
BmcAI AGTACT 2 cut(s) 263, 395
BmeT110I CYCGRG 1 cut(s) 327
BmrI ACTGGG 1 cut(s) 50
BmuI ACTGGG 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 413
BsaAI YACGTR 2 cut(s) 92, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 4 cut(s) 87, 381, 453, 586
BseNI ACTGG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 531
BseXI GCAGC 3 cut(s) 175, 270, 292
BshFI GGCC 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 531
BsiHKCI CYCGRG 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 61
BsnI GGCC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 1 cut(s) 480
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 4 cut(s) 88, 382, 454, 585
BsrI ACTGG 1 cut(s) 56
BssMI GATC 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 33, 366
Bst6I CTCTTC 1 cut(s) 545
BstBAI YACGTR 2 cut(s) 92, 116
BstC8I GCNNGC 2 cut(s) 208, 470
BstDEI CTNAG 4 cut(s) 96, 390, 462, 572
BstF5I GGATG 1 cut(s) 55
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 1 cut(s) 483
BstMBI GATC 1 cut(s) 480
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 531
BstSNI TACGTA 1 cut(s) 116
BstV1I GCAGC 3 cut(s) 175, 270, 292
BsuRI GGCC 1 cut(s) 183
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 371
Cac8I GCNNGC 2 cut(s) 208, 470
CaiI CAGNNNCTG 1 cut(s) 286
CfoI GCGC 1 cut(s) 499
Csp6I GTAC 3 cut(s) 262, 394, 569
CviAII CATG 2 cut(s) 175, 207
CviJI RGCY 8 cut(s) 101, 183, 188, 240, 247, 283, 326, 468
CviKI_1 RGCY 8 cut(s) 101, 183, 188, 240, 247, 283, 326, 468
CviQI GTAC 3 cut(s) 262, 394, 569
DdeI CTNAG 4 cut(s) 96, 390, 462, 572
DpnI GATC 1 cut(s) 482
DpnII GATC 1 cut(s) 480
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Eco105I TACGTA 1 cut(s) 116
Eco32I GATATC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 422, 440
Eco88I CYCGRG 1 cut(s) 327
EcoRV GATATC 1 cut(s) 445
FaeI CATG 2 cut(s) 178, 210
FatI CATG 2 cut(s) 174, 206
Fnu4HI GCNGC 3 cut(s) 189, 281, 284
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 3 cut(s) 189, 281, 284
FspBI CTAG 1 cut(s) 228
FspI TGCGCA 1 cut(s) 498
GlaI GCGC 1 cut(s) 498
GluI GCNGC 3 cut(s) 189, 281, 284
HaeIII GGCC 1 cut(s) 183
HhaI GCGC 1 cut(s) 499
Hin1II CATG 2 cut(s) 178, 210
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 5 cut(s) 8, 65, 424, 455, 558
Hpy166II GTNNAC 2 cut(s) 106, 529
Hpy188I TCNGA 2 cut(s) 439, 575
Hpy188III TCNNGA 3 cut(s) 145, 316, 329
Hpy8I GTNNAC 2 cut(s) 106, 529
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 2 cut(s) 33, 366
HpyCH4IV ACGT 3 cut(s) 81, 91, 115
HpyCH4V TGCA 7 cut(s) 210, 302, 472, 516, 529, 595, 631
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 4 cut(s) 96, 390, 462, 572
HpySE526I ACGT 3 cut(s) 81, 91, 115
Hsp92II CATG 2 cut(s) 178, 210
HspAI GCGC 1 cut(s) 497
Kzo9I GATC 1 cut(s) 480
LpnPI CCDG 7 cut(s) 69, 158, 200, 233, 272, 413, 617
Lsp1109I GCAGC 3 cut(s) 175, 270, 292
MaeI CTAG 1 cut(s) 228
MaeII ACGT 3 cut(s) 81, 91, 115
MaeIII GTNAC 1 cut(s) 154
MalI GATC 1 cut(s) 482
MboI GATC 1 cut(s) 480
MboII GAAGA 5 cut(s) 415, 433, 562, 567, 588
MhlI GDGCHC 1 cut(s) 531
MlsI TGGCCA 1 cut(s) 183
MluCI AATT 2 cut(s) 520, 534
MluNI TGGCCA 1 cut(s) 183
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 4 cut(s) 91, 118, 202, 445
Mox20I TGGCCA 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 41
MscI TGGCCA 1 cut(s) 183
MseI TTAA 3 cut(s) 243, 539, 545
Msp20I TGGCCA 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 283
MwoI GCNNNNNNNGC 1 cut(s) 237
NdeII GATC 1 cut(s) 480
NlaIII CATG 2 cut(s) 178, 210
NmuCI GTSAC 1 cut(s) 154
NsbI TGCGCA 1 cut(s) 498
NspI RCATGY 1 cut(s) 210
PaeI GCATGC 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 4 cut(s) 65, 424, 455, 558
PkrI GCNGC 3 cut(s) 190, 282, 285
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 2 cut(s) 92, 116
PshBI ATTAAT 1 cut(s) 545
PstNI CAGNNNCTG 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 3 cut(s) 263, 395, 570
RsaNI GTAC 3 cut(s) 262, 394, 569
SaqAI TTAA 3 cut(s) 243, 539, 545
SatI GCNGC 3 cut(s) 189, 281, 284
Sau3AI GATC 1 cut(s) 480
ScaI AGTACT 2 cut(s) 263, 395
SchI GAGTC 1 cut(s) 17
SduI GDGCHC 1 cut(s) 531
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SnaBI TACGTA 1 cut(s) 116
SphI GCATGC 1 cut(s) 210
Sse9I AATT 2 cut(s) 520, 534
SspMI CTAG 1 cut(s) 228
TaaI ACNGT 2 cut(s) 33, 366
TaiI ACGT 3 cut(s) 84, 94, 118
TaqI TCGA 1 cut(s) 224
TasI AATT 2 cut(s) 520, 534
TatI WGTACW 2 cut(s) 261, 393
TfiI GAWTC 4 cut(s) 65, 424, 455, 558
Tru1I TTAA 3 cut(s) 243, 539, 545
Tru9I TTAA 3 cut(s) 243, 539, 545
TscAI CASTG 1 cut(s) 371
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 3 cut(s) 188, 280, 283
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 3 cut(s) 42, 191, 563
TspRI CASTG 1 cut(s) 371
VneI GTGCAC 1 cut(s) 527
VspI ATTAAT 1 cut(s) 545
XapI RAATTY 2 cut(s) 520, 534
XceI RCATGY 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 228
ZrmI AGTACT 2 cut(s) 263, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.