Rorug06G0000300

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007486
Physical Location & Seq
Reverse (-)
153377 .. 161289
7913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0000300.1

Sequence Viewer

Length: 1083 bp
ATGCTTCGACCATGGCTACTGCTGTTAGTATTTTGTGTGCCTCTGCTACAACGGGTCAACTCGCACGAAGAGTCGGGTGAATGGAGCTGCGAGTCCGACTCAGACATCCGAATCCAGGCCCAGTTCGGGCCCGGCCTTATCACCCTCGATGGTCACGCCGACGATTGGAAGGACACGGATGGGTCCGAGTTCTCTCTTCTCCCTGCTCTTGACCCAGATGCTGAAACCGAATACACAGGTGGAAAGATGACCGTCAAGGCTTTGCATGATGGCCGTGATGTCTTCTTTATGGTTCAAGTTGATGGGGATTACGCTTACTCAAAAGGTGAGAATACCAAATGCCCATCTGTTGCCCTCATGTTTCAAATTGGCGAAGCTGCAACTTATCATAATATGGGCGGCTGTAAGGAAGGAGTTGACTCTTGCACAAACAAGACTTGCAAATCCCATGAAGTTGACATTATGCATTTCTCTATTGGAAATGCAATTCCTGGACGTCTCTATGGTGGCAACCCAGTGGACAACAGGGATGGAAATGGAGGTGACAGGTTTGGTCATTTGGTTGATCTTTATGGTTGGAACCCACACTGTAGATACCTAGATGGAATTAGCTCTTCAGCAAATGATTCTAGTGCACAAAATGATTGGAAAGGCGCTTGGTGGCACAGTAGCTTGAATGTTCACTCAGGTTTTGTGGAAGAAGATAGTCCTTATGCATCAGATGGTCAAAAGGGTACATACTATTTTGAATTCTCTAGGCCTTTGAGAACTATGGATCGCCTCCAACAGGATGCTCAATTCACAATTGGCGGATCAAGTAAGATGTCAGTTGCATTCTGGTATCCTGTTGATGGAAAACCATGGCATGGCTCTGGACACTATTCTGTTAGCTGTGACTGGGTTCCCTTGGATATATCTATGAAAATCTCTATGCTAACGAAGGTGAAACAAAGTAGTTCAGTCAGTGCTGCTAGTGTTTTTGCCCTTTTGCTATCAGTAATCTCATTATGTGCTTCCATTTTTATCGGGTATTGGGTTGTCAAACCCAAAACTATCCCCTTCACACCCATGGAAAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

360

Amino Acids

39.79

Weight (kDa)

5.08

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EB_dh PF09459 54 - 295 3.8e-39 Ethylbenzene dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 866
AciI CCGC 2 cut(s) 399, 810
AclWI GGATC 2 cut(s) 783, 820
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 1 cut(s) 749
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 1 cut(s) 736
AfiI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 787, 851, 866
AgsI TTSAA 4 cut(s) 296, 365, 676, 749
AjnI CCWGG 2 cut(s) 114, 490
AluBI AGCT 5 cut(s) 87, 377, 612, 672, 891
AluI AGCT 5 cut(s) 87, 377, 612, 672, 891
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 1 cut(s) 503
Alw44I GTGCAC 1 cut(s) 633
AlwI GGATC 2 cut(s) 783, 820
AlwNI CAGNNNCTG 1 cut(s) 221
AoxI GGCC 5 cut(s) 117, 128, 133, 271, 758
ApaI GGGCCC 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 3 cut(s) 87, 377, 968
ApoI RAATTY 1 cut(s) 749
AspLEI GCGC 1 cut(s) 656
AspS9I GGNCC 4 cut(s) 118, 128, 129, 183
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 5 cut(s) 89, 133, 338, 554, 955
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 2 cut(s) 132, 637
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 3 cut(s) 74, 364, 955
BccI CCATC 9 cut(s) 143, 173, 263, 296, 352, 524, 596, 716, 845
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 2 cut(s) 116, 492
BciVI GTATCC 1 cut(s) 852
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 503
BfaI CTAG 4 cut(s) 599, 630, 756, 972
BfmI CTRYAG 1 cut(s) 589
BfoI RGCGCY 1 cut(s) 657
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 4 cut(s) 88, 378, 400, 969
BlsI GCNGC 4 cut(s) 89, 379, 401, 970
Bme1390I CCNGG 3 cut(s) 116, 132, 492
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 4 cut(s) 118, 128, 129, 183
BmiI GGNNCC 4 cut(s) 130, 184, 581, 903
BmrFI CCNGG 3 cut(s) 116, 132, 492
BmrI ACTGGG 3 cut(s) 115, 509, 907
BmsI GCATC 3 cut(s) 208, 725, 781
BmuI ACTGGG 3 cut(s) 115, 509, 907
BpiI GAAGAC 1 cut(s) 274
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpuMI CCSGG 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 4 cut(s) 11, 860, 906, 1068
Bsc4I CCNNNNNNNGG 6 cut(s) 115, 126, 165, 787, 851, 866
Bse1I ACTGG 3 cut(s) 121, 515, 902
BseBI CCWGG 2 cut(s) 116, 492
BseDI CCNNGG 4 cut(s) 11, 860, 906, 1068
BseGI GGATG 4 cut(s) 105, 184, 535, 796
BseLI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 787, 851, 866
BseMII CTCAG 2 cut(s) 114, 699
BseNI ACTGG 3 cut(s) 121, 515, 902
BseSI GKGCMC 2 cut(s) 132, 637
BseXI GCAGC 3 cut(s) 74, 364, 955
BshFI GGCC 5 cut(s) 119, 130, 135, 273, 760
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 6 cut(s) 115, 126, 165, 787, 851, 866
BsmAI GTCTC 1 cut(s) 503
BsmBI CGTCTC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 833
BsnI GGCC 5 cut(s) 119, 130, 135, 273, 760
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 132, 637
Bsp143I GATC 3 cut(s) 565, 775, 812
Bsp19I CCATGG 3 cut(s) 11, 860, 1068
BspACI CCGC 2 cut(s) 399, 810
BspANI GGCC 5 cut(s) 119, 130, 135, 273, 760
BspCNI CTCAG 2 cut(s) 113, 698
BspLI GGNNCC 4 cut(s) 130, 184, 581, 903
BspPI GGATC 2 cut(s) 783, 820
BspQI GCTCTTC 1 cut(s) 619
BsrI ACTGG 3 cut(s) 121, 515, 902
BssECI CCNNGG 4 cut(s) 11, 860, 906, 1068
BssMI GATC 3 cut(s) 565, 775, 812
BssNI GRCGYC 1 cut(s) 496
BssT1I CCWWGG 4 cut(s) 11, 860, 906, 1068
Bst2UI CCWGG 2 cut(s) 116, 492
Bst4CI ACNGT 3 cut(s) 253, 590, 668
Bst6I CTCTTC 3 cut(s) 63, 201, 619
BstACI GRCGYC 1 cut(s) 496
BstDEI CTNAG 2 cut(s) 100, 685
BstDSI CCRYGG 3 cut(s) 11, 860, 1068
BstENI CCTNNNNNAGG 1 cut(s) 785
BstF5I GGATG 4 cut(s) 105, 184, 535, 796
BstH2I RGCGCY 1 cut(s) 657
BstHHI GCGC 1 cut(s) 656
BstKTI GATC 3 cut(s) 568, 778, 815
BstMAI GTCTC 1 cut(s) 503
BstMBI GATC 3 cut(s) 565, 775, 812
BstNI CCWGG 2 cut(s) 116, 492
BstSCI CCNGG 3 cut(s) 114, 130, 490
BstSFI CTRYAG 1 cut(s) 589
BstSLI GKGCMC 2 cut(s) 132, 637
BstV1I GCAGC 3 cut(s) 74, 364, 955
BstV2I GAAGAC 1 cut(s) 274
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 5 cut(s) 119, 130, 135, 273, 760
BtgI CCRYGG 3 cut(s) 11, 860, 1068
BtsCI GGATG 4 cut(s) 105, 184, 535, 796
BtsIMutI CAGTG 3 cut(s) 522, 586, 970
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 1 cut(s) 656
Cfr13I GGNCC 4 cut(s) 118, 128, 129, 183
Csp6I GTAC 1 cut(s) 735
CviAII CATG 7 cut(s) 12, 266, 358, 449, 861, 866, 1069
CviQI GTAC 1 cut(s) 735
DdeI CTNAG 2 cut(s) 100, 685
DpnI GATC 3 cut(s) 567, 777, 814
DpnII GATC 3 cut(s) 565, 775, 812
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 63, 201, 619
EarI CTCTTC 3 cut(s) 63, 201, 619
EciI GGCGGA 1 cut(s) 825
Eco130I CCWWGG 4 cut(s) 11, 860, 906, 1068
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 600
EcoNI CCTNNNNNAGG 1 cut(s) 785
EcoRI GAATTC 1 cut(s) 749
EcoRII CCWGG 2 cut(s) 114, 490
EcoT14I CCWWGG 4 cut(s) 11, 860, 906, 1068
EcoT22I ATGCAT 2 cut(s) 468, 718
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 4 cut(s) 11, 860, 906, 1068
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 7 cut(s) 15, 269, 361, 452, 864, 869, 1072
FatI CATG 7 cut(s) 11, 265, 357, 448, 860, 865, 1068
Fnu4HI GCNGC 4 cut(s) 88, 378, 400, 969
FokI GGATG 4 cut(s) 92, 191, 542, 803
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 4 cut(s) 88, 378, 400, 969
FspBI CTAG 4 cut(s) 599, 630, 756, 972
GlaI GCGC 1 cut(s) 655
GluI GCNGC 4 cut(s) 88, 378, 400, 969
HaeII RGCGCY 1 cut(s) 657
HaeIII GGCC 5 cut(s) 119, 130, 135, 273, 760
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 656
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 7 cut(s) 15, 269, 361, 452, 864, 869, 1072
Hin6I GCGC 1 cut(s) 654
HinP1I GCGC 1 cut(s) 654
HincII GTYRAC 3 cut(s) 58, 418, 457
HindII GTYRAC 3 cut(s) 58, 418, 457
HinfI GANTC 6 cut(s) 71, 92, 98, 111, 419, 626
HpaII CCGG 1 cut(s) 132
HphI GGTGA 5 cut(s) 89, 133, 338, 554, 955
Hpy166II GTNNAC 6 cut(s) 58, 418, 457, 520, 635, 682
Hpy188I TCNGA 5 cut(s) 97, 103, 110, 187, 721
Hpy188III TCNNGA 2 cut(s) 209, 873
Hpy8I GTNNAC 6 cut(s) 58, 418, 457, 520, 635, 682
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 4 cut(s) 163, 404, 934, 1069
HpyCH4III ACNGT 3 cut(s) 253, 590, 668
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 9 cut(s) 265, 380, 426, 441, 466, 485, 635, 716, 833
HpyF3I CTNAG 2 cut(s) 100, 685
HpySE526I ACGT 1 cut(s) 496
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 7 cut(s) 15, 269, 361, 452, 864, 869, 1072
HspAI GCGC 1 cut(s) 654
Kzo9I GATC 3 cut(s) 565, 775, 812
LguI GCTCTTC 1 cut(s) 619
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 3 cut(s) 74, 364, 955
LweI GCATC 3 cut(s) 208, 725, 781
MaeI CTAG 4 cut(s) 599, 630, 756, 972
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 3 cut(s) 152, 542, 893
MalI GATC 3 cut(s) 567, 777, 814
MboI GATC 3 cut(s) 565, 775, 812
MboII GAAGA 6 cut(s) 80, 188, 274, 606, 710, 713
MfeI CAATTG 1 cut(s) 804
MhlI GDGCHC 2 cut(s) 132, 637
MluCI AATT 6 cut(s) 366, 486, 606, 749, 797, 804
MlyI GAGTC 4 cut(s) 80, 92, 101, 413
MmeI TCCRAC 3 cut(s) 120, 557, 808
MnlI CCTC 5 cut(s) 51, 155, 365, 533, 791
Mph1103I ATGCAT 2 cut(s) 468, 718
MseI TTAA 1 cut(s) 1081
MslI CAYNNNNRTG 1 cut(s) 1067
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 116, 132, 492
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 833
MvaI CCWGG 2 cut(s) 116, 492
NciI CCSGG 1 cut(s) 132
NcoI CCATGG 3 cut(s) 11, 860, 1068
NdeII GATC 3 cut(s) 565, 775, 812
NlaIII CATG 7 cut(s) 15, 269, 361, 452, 864, 869, 1072
NlaIV GGNNCC 4 cut(s) 130, 184, 581, 903
NmuCI GTSAC 3 cut(s) 152, 542, 893
NsiI ATGCAT 2 cut(s) 468, 718
PceI AGGCCT 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 619
PctI GAATGC 1 cut(s) 833
PfeI GAWTC 2 cut(s) 111, 626
PflMI CCANNNNNTGG 1 cut(s) 866
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 4 cut(s) 89, 379, 401, 970
PleI GAGTC 4 cut(s) 79, 92, 100, 413
PpsI GAGTC 4 cut(s) 79, 92, 100, 413
Psp6I CCWGG 2 cut(s) 114, 490
PspGI CCWGG 2 cut(s) 114, 490
PspN4I GGNNCC 4 cut(s) 130, 184, 581, 903
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 4 cut(s) 118, 128, 129, 183
PstNI CAGNNNCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 736
RsaNI GTAC 1 cut(s) 735
RseI CAYNNNNRTG 1 cut(s) 1067
SapI GCTCTTC 1 cut(s) 619
SaqAI TTAA 1 cut(s) 1081
SatI GCNGC 4 cut(s) 88, 378, 400, 969
Sau3AI GATC 3 cut(s) 565, 775, 812
Sau96I GGNCC 4 cut(s) 118, 128, 129, 183
SchI GAGTC 4 cut(s) 80, 92, 101, 413
ScrFI CCNGG 3 cut(s) 116, 132, 492
SduI GDGCHC 2 cut(s) 132, 637
SfaNI GCATC 3 cut(s) 208, 725, 781
SfcI CTRYAG 1 cut(s) 589
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 1067
Sse9I AATT 6 cut(s) 366, 486, 606, 749, 797, 804
SseBI AGGCCT 1 cut(s) 760
SsiI CCGC 2 cut(s) 399, 810
SspMI CTAG 4 cut(s) 599, 630, 756, 972
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 3 cut(s) 114, 130, 490
StyI CCWWGG 4 cut(s) 11, 860, 906, 1068
TaaI ACNGT 3 cut(s) 253, 590, 668
TaiI ACGT 1 cut(s) 499
TaqI TCGA 2 cut(s) 7, 147
TasI AATT 6 cut(s) 366, 486, 606, 749, 797, 804
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 2 cut(s) 111, 626
Tru1I TTAA 1 cut(s) 1081
Tru9I TTAA 1 cut(s) 1081
TscAI CASTG 3 cut(s) 522, 593, 970
TseFI GTSAC 3 cut(s) 152, 542, 893
TseI GCWGC 3 cut(s) 87, 377, 968
Tsp45I GTSAC 3 cut(s) 152, 542, 893
TspDTI ATGAA 2 cut(s) 465, 935
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 3 cut(s) 522, 593, 970
Van91I CCANNNNNTGG 1 cut(s) 866
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 1 cut(s) 183
XagI CCTNNNNNAGG 1 cut(s) 785
XapI RAATTY 1 cut(s) 749
XspI CTAG 4 cut(s) 599, 630, 756, 972
ZraI GACGTC 1 cut(s) 497
Zsp2I ATGCAT 2 cut(s) 468, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.