Rroxscaffold_7G00205010

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007486
Physical Location & Seq
Reverse (-)
54486399 .. 54493400
7002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205010.1

Sequence Viewer

Length: 525 bp
ATGGGCCCGGACGTTGCGCAGGGTTCAGCTACGTACCGCAAGGGAATTGAAAAAAACAGGCCTGCTAGTGGTAATAAATGGGTTGAGCGACCCGCGATATCATTGCAAAGCCTAGCCAACAACCTTTCCGGTCAAGAATTTCGGCATCTTTTCAAGCAAAAATGGGAGTCATATAAGAAGAAGCATAAGGAACAGTTGAAATCATTCAAGGATGACCCAGACGTGAAACTCGCCAACAACATAAGAATACGTGCTGAGGCTGCCTATAACGTAAGTAAGAAGGAGGGTTTTTCTCTAGCTTTTCTGAAAGAAGTGACGGCATTTGTTTCCTACATGAATGGCCAAGCTGCCTCTACAATCTTGACATGCGAACAGGATATATCAACTAGCAACAAGAGCTTTAAGTTGGTTGTCAATGAGTACTTGAGCAATAGTGAGCAGCTGCTGGATTTCTGTTCCGCATTGGATAAGTTCGTGAAGTTAGCTCGGGATGGTCATTCATATACACAATATGTGGTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

174

Amino Acids

19.7

Weight (kDa)

9.34

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 94 - 172 4.3e-10 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 18
AccII CGCG 1 cut(s) 95
AciI CCGC 3 cut(s) 37, 93, 459
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 2 cut(s) 35, 422
AfiI CCNNNNNNNGG 1 cut(s) 68
AgsI TTSAA 5 cut(s) 50, 154, 199, 208, 521
AjiI CACGTC 1 cut(s) 223
AluBI AGCT 6 cut(s) 29, 299, 347, 399, 442, 485
AluI AGCT 6 cut(s) 29, 299, 347, 399, 442, 485
AlwNI CAGNNNCTG 1 cut(s) 445
Ama87I CYCGRG 1 cut(s) 486
AoxI GGCC 3 cut(s) 4, 59, 340
ApaI GGGCCC 1 cut(s) 8
ApeKI GCWGC 4 cut(s) 260, 347, 439, 442
ApoI RAATTY 1 cut(s) 137
Asp700I GAANNNNTTC 1 cut(s) 203
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 4, 5
AsuC2I CCSGG 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 486
BaeGI GKGCMC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 342
BanII GRGCYC 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 4 cut(s) 247, 334, 429, 451
BccI CCATC 1 cut(s) 485
BceAI ACGGC 1 cut(s) 333
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BcnI CCSGG 1 cut(s) 8
BfaI CTAG 4 cut(s) 66, 113, 296, 387
BisI GCNGC 4 cut(s) 261, 348, 440, 443
BlsI GCNGC 4 cut(s) 262, 349, 441, 444
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 8
BmeT110I CYCGRG 1 cut(s) 486
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 4, 5
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 8
BmsI GCATC 1 cut(s) 154
Bpu10I CCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 8
BsaAI YACGTR 2 cut(s) 33, 251
BsaWI WCCGGW 1 cut(s) 128
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 101
BseGI GGATG 2 cut(s) 217, 496
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMI GCAATG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 4 cut(s) 247, 334, 429, 451
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 3 cut(s) 6, 61, 342
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 2 cut(s) 8, 129
BslI CCNNNNNNNGG 1 cut(s) 68
BsnI GGCC 3 cut(s) 6, 61, 342
BsoBI CYCGRG 1 cut(s) 486
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 8
BspACI CCGC 3 cut(s) 37, 93, 459
BspANI GGCC 3 cut(s) 6, 61, 342
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 101
Bst4CI ACNGT 1 cut(s) 195
BstBAI YACGTR 2 cut(s) 33, 251
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 217, 496
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 19
BstMWI GCNNNNNNNGC 2 cut(s) 260, 396
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 8
BstSNI TACGTA 1 cut(s) 33
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 4 cut(s) 247, 334, 429, 451
BsuRI GGCC 3 cut(s) 6, 61, 342
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 217, 496
Cac8I GCNNGC 1 cut(s) 63
CaiI CAGNNNCTG 1 cut(s) 445
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 4, 5
Csp6I GTAC 2 cut(s) 34, 421
CviAII CATG 2 cut(s) 334, 366
CviQI GTAC 2 cut(s) 34, 421
DdeI CTNAG 1 cut(s) 255
EaeI YGGCCR 1 cut(s) 340
Eco105I TACGTA 1 cut(s) 33
Eco147I AGGCCT 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 8
Eco32I GATATC 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 486
EcoRV GATATC 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 2 cut(s) 337, 369
FatI CATG 2 cut(s) 333, 365
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 4 cut(s) 261, 348, 440, 443
FokI GGATG 2 cut(s) 224, 503
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 261, 348, 440, 443
FspBI CTAG 4 cut(s) 66, 113, 296, 387
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 1 cut(s) 18
GluI GCNGC 4 cut(s) 261, 348, 440, 443
HaeIII GGCC 3 cut(s) 6, 61, 342
HapII CCGG 2 cut(s) 8, 129
HhaI GCGC 1 cut(s) 19
Hin1II CATG 2 cut(s) 337, 369
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 1 cut(s) 167
HpaII CCGG 2 cut(s) 8, 129
Hpy188I TCNGA 1 cut(s) 306
Hpy188III TCNNGA 4 cut(s) 134, 361, 475, 488
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 195
HpyCH4IV ACGT 5 cut(s) 12, 32, 222, 250, 270
HpyCH4V TGCA 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 396
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 5 cut(s) 12, 32, 222, 250, 270
Hsp92II CATG 2 cut(s) 337, 369
HspAI GCGC 1 cut(s) 17
LpnPI CCDG 8 cut(s) 5, 21, 43, 75, 142, 231, 359, 431
Lsp1109I GCAGC 4 cut(s) 247, 334, 429, 451
LweI GCATC 1 cut(s) 154
MaeI CTAG 4 cut(s) 66, 113, 296, 387
MaeII ACGT 5 cut(s) 12, 32, 222, 250, 270
MaeIII GTNAC 1 cut(s) 313
MboII GAAGA 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 2 cut(s) 45, 137
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 1 cut(s) 176
MnlI CCTC 3 cut(s) 250, 277, 361
Mox20I TGGCCA 1 cut(s) 342
MroXI GAANNNNTTC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 342
MseI TTAA 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 342
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 2 cut(s) 8, 129
MspR9I CCNGG 1 cut(s) 8
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 260, 396
NciI CCSGG 1 cut(s) 8
NlaIII CATG 2 cut(s) 337, 369
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 313
NsbI TGCGCA 1 cut(s) 18
NspI RCATGY 1 cut(s) 369
PceI AGGCCT 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 203
PkrI GCNGC 4 cut(s) 262, 349, 441, 444
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 2 cut(s) 33, 251
PspN4I GGNNCC 1 cut(s) 6
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 2 cut(s) 4, 5
PstNI CAGNNNCTG 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 442
RsaI GTAC 2 cut(s) 35, 422
RsaNI GTAC 2 cut(s) 34, 421
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 4 cut(s) 261, 348, 440, 443
Sau96I GGNCC 2 cut(s) 4, 5
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 8
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 1 cut(s) 154
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SnaBI TACGTA 1 cut(s) 33
Sse9I AATT 2 cut(s) 45, 137
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 3 cut(s) 37, 93, 459
SspMI CTAG 4 cut(s) 66, 113, 296, 387
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 195
TaiI ACGT 5 cut(s) 15, 35, 225, 253, 273
TasI AATT 2 cut(s) 45, 137
TatI WGTACW 1 cut(s) 420
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 4 cut(s) 260, 347, 439, 442
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 2 cut(s) 350, 489
XapI RAATTY 1 cut(s) 137
XceI RCATGY 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 4 cut(s) 66, 113, 296, 387
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.