RLG00000014409

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007486
Physical Location & Seq
Reverse (-)
52836378 .. 52837203
826 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014409

Sequence Viewer

Length: 675 bp
ATGGAAACTGAGTACTGCCTGAAGATTTTGGAGAAATTGAAGAATCTCAAGTCCTCTGATGAGATCCATGATAATTCTGCTGAAAGCTTGCACCAAAAGATCAAAGGACTTGTAGGTGCGGATTTCTTACCCGATTTAGCAGACATTGTTTCCGGTATTGCAGAAATTGCTTCTGATGTTGCAGATGCTGCTTCTAATACAGCTGATGCTGCTACTCATCCACATATTGAGTCAGTACAAGCAACATCCTTAGAATGTATTAATAATCATGAGACTGCTTCAAAGTATGCTGTCAGGGCCGGGTTTACAGCAGTTGCTGCAGTACCTTTAAACTTAGGGCATTCATTAGTGATGTCTCATTATGATAATAAACAAAAGGATTTGGACAACAAGAAGAAGGACATGGACGCAATACGTATAGAAAGTGAGTCATCCATTACGGAGTTGGGCAACATTAAGTTTCGCATTAATCAGGTGGTAAACGGGATTGATTCACTACTGGCCAAAACCAACACTGCAGTTAAAATGAGTGACATAGATTTCTTAATGAAGGATATGAAGCAAAAGCTGGAGATGTTCATGAAGAAAATTAACGATTTGGATGCAGAAGCAGTGAAATGCAGCGCTGAAACATTGAAGGCAAGGGATGACGTGCGCAGCAGAATCAGACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

225

Amino Acids

24.67

Weight (kDa)

5.53

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 100 - 222 1.9e-11 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 656
AciI CCGC 1 cut(s) 119
AclWI GGATC 1 cut(s) 58
AcoI YGGCCR 1 cut(s) 501
AcuI CTGAAG 1 cut(s) 41
AfaI GTAC 3 cut(s) 14, 237, 324
AfeI AGCGCT 1 cut(s) 625
AgsI TTSAA 3 cut(s) 40, 282, 637
AjiI CACGTC 1 cut(s) 652
AluBI AGCT 3 cut(s) 87, 203, 568
AluI AGCT 3 cut(s) 87, 203, 568
Alw26I GTCTC 2 cut(s) 266, 360
AlwI GGATC 1 cut(s) 58
AlwNI CAGNNNCTG 2 cut(s) 188, 317
Aor51HI AGCGCT 1 cut(s) 625
AoxI GGCC 2 cut(s) 297, 501
ApeKI GCWGC 5 cut(s) 188, 209, 317, 621, 657
AseI ATTAAT 2 cut(s) 261, 468
AspLEI GCGC 2 cut(s) 626, 657
AspS9I GGNCC 1 cut(s) 297
AsuC2I CCSGG 1 cut(s) 301
BalI TGGCCA 1 cut(s) 503
BbvI GCAGC 5 cut(s) 175, 196, 304, 633, 669
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 2 cut(s) 266, 360
BfmI CTRYAG 2 cut(s) 318, 516
BfoI RGCGCY 1 cut(s) 627
BisI GCNGC 5 cut(s) 189, 210, 318, 622, 658
BlsI GCNGC 5 cut(s) 190, 211, 319, 623, 659
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 301
BmgBI CACGTC 1 cut(s) 652
BmgT120I GGNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 3 cut(s) 175, 196, 592
BpmI CTGGAG 1 cut(s) 590
BpuEI CTTGAG 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 301
BsaAI YACGTR 1 cut(s) 416
BsaWI WCCGGW 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 504
BseGI GGATG 5 cut(s) 217, 245, 431, 607, 652
BseNI ACTGG 1 cut(s) 504
BseXI GCAGC 5 cut(s) 175, 196, 304, 633, 669
BshFI GGCC 2 cut(s) 299, 503
BsiSI CCGG 2 cut(s) 153, 300
BsmAI GTCTC 2 cut(s) 266, 360
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 2 cut(s) 299, 503
Bsp143I GATC 2 cut(s) 63, 99
BspACI CCGC 1 cut(s) 119
BspANI GGCC 2 cut(s) 299, 503
BspHI TCATGA 2 cut(s) 268, 579
BspMAI CTGCAG 2 cut(s) 322, 520
BspPI GGATC 1 cut(s) 58
BsrI ACTGG 1 cut(s) 504
BssMI GATC 2 cut(s) 63, 99
BstAPI GCANNNNNTGC 3 cut(s) 167, 188, 317
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 3 cut(s) 9, 250, 334
BstF5I GGATG 5 cut(s) 217, 245, 431, 607, 652
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 2 cut(s) 626, 657
BstKTI GATC 2 cut(s) 66, 102
BstMAI GTCTC 2 cut(s) 266, 360
BstMBI GATC 2 cut(s) 63, 99
BstMWI GCNNNNNNNGC 5 cut(s) 167, 188, 209, 296, 317
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 2 cut(s) 318, 516
BstSNI TACGTA 1 cut(s) 416
BstV1I GCAGC 5 cut(s) 175, 196, 304, 633, 669
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
BsuRI GGCC 2 cut(s) 299, 503
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 5 cut(s) 217, 245, 431, 607, 652
BtsI GCAGTG 2 cut(s) 513, 618
BtsIMutI CAGTG 2 cut(s) 513, 618
Cac8I GCNNGC 1 cut(s) 89
CaiI CAGNNNCTG 2 cut(s) 188, 317
CciI TCATGA 2 cut(s) 268, 579
CfoI GCGC 2 cut(s) 626, 657
Cfr13I GGNCC 1 cut(s) 297
CseI GACGC 1 cut(s) 416
Csp6I GTAC 3 cut(s) 13, 236, 323
CviAII CATG 4 cut(s) 68, 269, 403, 580
CviJI RGCY 5 cut(s) 87, 203, 299, 503, 568
CviKI_1 RGCY 5 cut(s) 87, 203, 299, 503, 568
CviQI GTAC 3 cut(s) 13, 236, 323
DdeI CTNAG 3 cut(s) 9, 250, 334
DpnI GATC 2 cut(s) 65, 101
DpnII GATC 2 cut(s) 63, 99
DraI TTTAAA 1 cut(s) 330
EaeI YGGCCR 1 cut(s) 501
Eco105I TACGTA 1 cut(s) 416
Eco47III AGCGCT 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 41
FaeI CATG 4 cut(s) 71, 272, 406, 583
FatI CATG 4 cut(s) 67, 268, 402, 579
Fnu4HI GCNGC 5 cut(s) 189, 210, 318, 622, 658
FokI GGATG 5 cut(s) 204, 232, 418, 614, 659
Fsp4HI GCNGC 5 cut(s) 189, 210, 318, 622, 658
FspI TGCGCA 1 cut(s) 656
GlaI GCGC 2 cut(s) 625, 656
GluI GCNGC 5 cut(s) 189, 210, 318, 622, 658
GsuI CTGGAG 1 cut(s) 590
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 2 cut(s) 299, 503
HapII CCGG 2 cut(s) 153, 300
HgaI GACGC 1 cut(s) 416
HhaI GCGC 2 cut(s) 626, 657
Hin1II CATG 4 cut(s) 71, 272, 406, 583
Hin6I GCGC 2 cut(s) 624, 655
HinP1I GCGC 2 cut(s) 624, 655
HindIII AAGCTT 1 cut(s) 85
HinfI GANTC 6 cut(s) 43, 230, 428, 491, 663, 669
HpaII CCGG 2 cut(s) 153, 300
Hpy166II GTNNAC 2 cut(s) 306, 481
Hpy188I TCNGA 3 cut(s) 58, 175, 668
Hpy188III TCNNGA 2 cut(s) 269, 580
Hpy8I GTNNAC 2 cut(s) 306, 481
HpyAV CCTTC 3 cut(s) 391, 544, 631
HpyCH4IV ACGT 2 cut(s) 415, 651
HpyCH4V TGCA 7 cut(s) 91, 161, 182, 320, 518, 605, 621
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 188, 209, 296, 317
HpyF3I CTNAG 3 cut(s) 9, 250, 334
HpySE526I ACGT 2 cut(s) 415, 651
Hsp92II CATG 4 cut(s) 71, 272, 406, 583
HspAI GCGC 2 cut(s) 624, 655
Kzo9I GATC 2 cut(s) 63, 99
LpnPI CCDG 7 cut(s) 32, 166, 280, 313, 458, 485, 554
Lsp1109I GCAGC 5 cut(s) 175, 196, 304, 633, 669
LweI GCATC 3 cut(s) 175, 196, 592
MaeII ACGT 2 cut(s) 415, 651
MaeIII GTNAC 1 cut(s) 530
MalI GATC 2 cut(s) 65, 101
MboI GATC 2 cut(s) 63, 99
MboII GAAGA 4 cut(s) 34, 52, 406, 595
MflI RGATCY 1 cut(s) 63
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 4 cut(s) 35, 73, 165, 588
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 3 cut(s) 239, 437, 663
MnlI CCTC 1 cut(s) 64
Mox20I TGGCCA 1 cut(s) 503
MscI TGGCCA 1 cut(s) 503
MseI TTAA 7 cut(s) 261, 329, 456, 468, 522, 545, 591
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 2 cut(s) 153, 300
MspR9I CCNGG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 340
MwoI GCNNNNNNNGC 5 cut(s) 167, 188, 209, 296, 317
NciI CCSGG 1 cut(s) 301
NdeII GATC 2 cut(s) 63, 99
NlaIII CATG 4 cut(s) 71, 272, 406, 583
NmuCI GTSAC 1 cut(s) 530
NsbI TGCGCA 1 cut(s) 656
PagI TCATGA 2 cut(s) 268, 579
PctI GAATGC 1 cut(s) 340
PfeI GAWTC 3 cut(s) 43, 491, 663
PkrI GCNGC 5 cut(s) 190, 211, 319, 623, 659
PleI GAGTC 3 cut(s) 238, 436, 663
PpsI GAGTC 3 cut(s) 238, 436, 663
Ppu21I YACGTR 1 cut(s) 416
PshBI ATTAAT 2 cut(s) 261, 468
PspPI GGNCC 1 cut(s) 297
PstI CTGCAG 2 cut(s) 322, 520
PstNI CAGNNNCTG 2 cut(s) 188, 317
PsuI RGATCY 1 cut(s) 63
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 3 cut(s) 14, 237, 324
RsaNI GTAC 3 cut(s) 13, 236, 323
SaqAI TTAA 7 cut(s) 261, 329, 456, 468, 522, 545, 591
SatI GCNGC 5 cut(s) 189, 210, 318, 622, 658
Sau3AI GATC 2 cut(s) 63, 99
Sau96I GGNCC 1 cut(s) 297
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 3 cut(s) 239, 437, 663
ScrFI CCNGG 1 cut(s) 301
SetI ASST 8 cut(s) 89, 118, 205, 328, 418, 477, 570, 654
SfaNI GCATC 3 cut(s) 175, 196, 592
SfcI CTRYAG 2 cut(s) 318, 516
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
SnaBI TACGTA 1 cut(s) 416
Sse9I AATT 4 cut(s) 35, 73, 165, 588
SsiI CCGC 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 299
TaiI ACGT 2 cut(s) 418, 654
TasI AATT 4 cut(s) 35, 73, 165, 588
TatI WGTACW 2 cut(s) 12, 235
TfiI GAWTC 3 cut(s) 43, 491, 663
Tru1I TTAA 7 cut(s) 261, 329, 456, 468, 522, 545, 591
Tru9I TTAA 7 cut(s) 261, 329, 456, 468, 522, 545, 591
TscAI CASTG 2 cut(s) 520, 618
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 5 cut(s) 188, 209, 317, 621, 657
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 5 cut(s) 333, 563, 568, 572, 596
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 2 cut(s) 520, 618
VspI ATTAAT 2 cut(s) 261, 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
ZrmI AGTACT 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.