Rh6CG112100

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0007486
Physical Location & Seq
Forward (+)
12870570 .. 12871624
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG112100.1

Sequence Viewer

Length: 747 bp
ATGAATGGCCAAGCTGCCTCTGCAATCTTGACATGCGAACAGGATATATCAACTAGCAACAAGAGCTTTAAGTTGGTTGTCAATGAGTACTTGAGCAATGAGCAGCTGCTGGATTTCTGTTCCGCATTGGATAAGTTCGTGAAGTTAGCTCGGGATGGACTTGTAGGTGCGGATTTCTTACCCGATTTAGCAGACATTGTTTCCGGTATTGCAGAAATTGCTTCTGATGTTGCAGATGCTGCTTCTAATACAGCTGATGCTGCTTCATGTGCAGCTGATGCTGCTACTCATCCACATATTGAGTCAGTACAAGCAACATCCTTAGAATGTATTAATAATCATGAGACTGCTTCAAAGTATGCTGTCAGGGTCGGGTTTACAGCAGTTGCTGCAGTACCTTTAAACTTAGGGCATTCATTAGTGATGTCTCATTATGATAATAAACAAAAGGATTTGGACAACAAGAAGAAGGACACGGACGCAATACGTAGAGAAAGTGAGTCATCCATTACGGAGTTGGGCAACATTAAGTTTCGCATTAATCAGGTGGTAAACGAGATTGATTCACTACTGGCCAAAACCAACACTGCAGTTAAAATGAGTGACCTAGATTTCTTAATGAAGGATATGAAGCAAAAGCTGGAGATGTTCATGAAGAAAATTAACGATTTGGATGCAGAAGCAGTGAAATGCAGCGCTGAAACATTGAAGGCAAGGGATGACGTGCGCAACAGAATCAGACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

248

Amino Acids

27.06

Weight (kDa)

5.1

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 123 - 246 1.5e-11 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 728
AciI CCGC 2 cut(s) 123, 170
AcoI YGGCCR 2 cut(s) 7, 573
AfaI GTAC 3 cut(s) 89, 309, 396
AfeI AGCGCT 1 cut(s) 697
AgsI TTSAA 2 cut(s) 354, 709
AjiI CACGTC 1 cut(s) 724
AluBI AGCT 7 cut(s) 14, 66, 106, 149, 254, 275, 640
AluI AGCT 7 cut(s) 14, 66, 106, 149, 254, 275, 640
Alw26I GTCTC 2 cut(s) 338, 432
AlwNI CAGNNNCTG 3 cut(s) 109, 239, 389
Ama87I CYCGRG 1 cut(s) 150
Aor51HI AGCGCT 1 cut(s) 697
AoxI GGCC 2 cut(s) 7, 573
ApeKI GCWGC 9 cut(s) 14, 103, 106, 239, 260, 272, 281, 389, 693
AseI ATTAAT 2 cut(s) 333, 540
AspLEI GCGC 2 cut(s) 698, 729
AvaI CYCGRG 1 cut(s) 150
BalI TGGCCA 2 cut(s) 9, 575
BbvI GCAGC 8 cut(s) 93, 115, 226, 247, 268, 284, 376, 705
BccI CCATC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BcoDI GTCTC 2 cut(s) 338, 432
BfaI CTAG 2 cut(s) 54, 608
BfmI CTRYAG 2 cut(s) 390, 588
BfoI RGCGCY 1 cut(s) 699
BisI GCNGC 9 cut(s) 15, 104, 107, 240, 261, 273, 282, 390, 694
BlsI GCNGC 9 cut(s) 16, 105, 108, 241, 262, 274, 283, 391, 695
BmcAI AGTACT 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 150
BmgBI CACGTC 1 cut(s) 724
BmsI GCATC 4 cut(s) 226, 247, 268, 664
BpmI CTGGAG 1 cut(s) 662
BpuEI CTTGAG 1 cut(s) 112
BsaAI YACGTR 1 cut(s) 488
BsaWI WCCGGW 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 576
Bse3DI GCAATG 1 cut(s) 103
BseGI GGATG 6 cut(s) 160, 289, 317, 503, 679, 724
BseMI GCAATG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 576
BseXI GCAGC 8 cut(s) 93, 115, 226, 247, 268, 284, 376, 705
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 2 cut(s) 9, 575
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 204
BsmAI GTCTC 2 cut(s) 338, 432
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 2 cut(s) 9, 575
BsoBI CYCGRG 1 cut(s) 150
BspACI CCGC 2 cut(s) 123, 170
BspANI GGCC 2 cut(s) 9, 575
BspHI TCATGA 2 cut(s) 340, 651
BspMAI CTGCAG 2 cut(s) 394, 592
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 1 cut(s) 576
BstAPI GCANNNNNTGC 4 cut(s) 218, 239, 278, 389
BstBAI YACGTR 1 cut(s) 488
BstDEI CTNAG 2 cut(s) 322, 406
BstF5I GGATG 6 cut(s) 160, 289, 317, 503, 679, 724
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 2 cut(s) 698, 729
BstMAI GTCTC 2 cut(s) 338, 432
BstMWI GCNNNNNNNGC 9 cut(s) 20, 63, 218, 239, 260, 269, 278, 281, 389
BstNSI RCATGY 1 cut(s) 36
BstSFI CTRYAG 2 cut(s) 390, 588
BstSNI TACGTA 1 cut(s) 488
BstV1I GCAGC 8 cut(s) 93, 115, 226, 247, 268, 284, 376, 705
BsuRI GGCC 2 cut(s) 9, 575
BtrI CACGTC 1 cut(s) 724
BtsCI GGATG 6 cut(s) 160, 289, 317, 503, 679, 724
BtsI GCAGTG 2 cut(s) 585, 690
BtsIMutI CAGTG 2 cut(s) 585, 690
CaiI CAGNNNCTG 3 cut(s) 109, 239, 389
CciI TCATGA 2 cut(s) 340, 651
CfoI GCGC 2 cut(s) 698, 729
CseI GACGC 1 cut(s) 488
Csp6I GTAC 3 cut(s) 88, 308, 395
CviAII CATG 4 cut(s) 33, 267, 341, 652
CviJI RGCY 9 cut(s) 9, 14, 66, 106, 149, 254, 275, 575, 640
CviKI_1 RGCY 9 cut(s) 9, 14, 66, 106, 149, 254, 275, 575, 640
CviQI GTAC 3 cut(s) 88, 308, 395
DdeI CTNAG 2 cut(s) 322, 406
DraI TTTAAA 1 cut(s) 402
EaeI YGGCCR 2 cut(s) 7, 573
Eco105I TACGTA 1 cut(s) 488
Eco47III AGCGCT 1 cut(s) 697
Eco88I CYCGRG 1 cut(s) 150
FaeI CATG 4 cut(s) 36, 270, 344, 655
FaiI YATR 9 cut(s) 34, 47, 268, 297, 342, 360, 435, 629, 653
FatI CATG 4 cut(s) 32, 266, 340, 651
Fnu4HI GCNGC 9 cut(s) 15, 104, 107, 240, 261, 273, 282, 390, 694
FokI GGATG 6 cut(s) 167, 276, 304, 490, 686, 731
Fsp4HI GCNGC 9 cut(s) 15, 104, 107, 240, 261, 273, 282, 390, 694
FspBI CTAG 2 cut(s) 54, 608
FspI TGCGCA 1 cut(s) 728
GlaI GCGC 2 cut(s) 697, 728
GluI GCNGC 9 cut(s) 15, 104, 107, 240, 261, 273, 282, 390, 694
GsuI CTGGAG 1 cut(s) 662
HaeII RGCGCY 1 cut(s) 699
HaeIII GGCC 2 cut(s) 9, 575
HapII CCGG 1 cut(s) 204
HgaI GACGC 1 cut(s) 488
HhaI GCGC 2 cut(s) 698, 729
Hin1II CATG 4 cut(s) 36, 270, 344, 655
Hin6I GCGC 2 cut(s) 696, 727
HinP1I GCGC 2 cut(s) 696, 727
HinfI GANTC 5 cut(s) 302, 500, 563, 735, 741
HpaII CCGG 1 cut(s) 204
Hpy166II GTNNAC 2 cut(s) 378, 553
Hpy188I TCNGA 2 cut(s) 226, 740
Hpy188III TCNNGA 5 cut(s) 28, 139, 152, 341, 652
Hpy8I GTNNAC 2 cut(s) 378, 553
HpyAV CCTTC 3 cut(s) 463, 616, 703
HpyCH4IV ACGT 2 cut(s) 487, 723
HpyCH4V TGCA 8 cut(s) 23, 212, 233, 272, 392, 590, 677, 693
HpyF10VI GCNNNNNNNGC 9 cut(s) 20, 63, 218, 239, 260, 269, 278, 281, 389
HpyF3I CTNAG 2 cut(s) 322, 406
HpySE526I ACGT 2 cut(s) 487, 723
Hsp92II CATG 4 cut(s) 36, 270, 344, 655
HspAI GCGC 2 cut(s) 696, 727
LpnPI CCDG 7 cut(s) 26, 95, 217, 352, 530, 557, 626
Lsp1109I GCAGC 8 cut(s) 93, 115, 226, 247, 268, 284, 376, 705
LweI GCATC 4 cut(s) 226, 247, 268, 664
MaeI CTAG 2 cut(s) 54, 608
MaeII ACGT 2 cut(s) 487, 723
MaeIII GTNAC 1 cut(s) 602
MboII GAAGA 2 cut(s) 478, 667
MlsI TGGCCA 2 cut(s) 9, 575
MluCI AATT 2 cut(s) 216, 660
MluNI TGGCCA 2 cut(s) 9, 575
MlyI GAGTC 3 cut(s) 311, 509, 735
MnlI CCTC 1 cut(s) 28
Mox20I TGGCCA 2 cut(s) 9, 575
MscI TGGCCA 2 cut(s) 9, 575
MseI TTAA 8 cut(s) 69, 333, 401, 528, 540, 594, 617, 663
Msp20I TGGCCA 2 cut(s) 9, 575
MspA1I CMGCKG 3 cut(s) 106, 254, 275
MspI CCGG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 412
MwoI GCNNNNNNNGC 9 cut(s) 20, 63, 218, 239, 260, 269, 278, 281, 389
NlaIII CATG 4 cut(s) 36, 270, 344, 655
NmuCI GTSAC 1 cut(s) 602
NsbI TGCGCA 1 cut(s) 728
NspI RCATGY 1 cut(s) 36
PagI TCATGA 2 cut(s) 340, 651
PctI GAATGC 1 cut(s) 412
PfeI GAWTC 2 cut(s) 563, 735
PkrI GCNGC 9 cut(s) 16, 105, 108, 241, 262, 274, 283, 391, 695
PleI GAGTC 3 cut(s) 310, 508, 735
PpsI GAGTC 3 cut(s) 310, 508, 735
Ppu21I YACGTR 1 cut(s) 488
PshBI ATTAAT 2 cut(s) 333, 540
PstI CTGCAG 2 cut(s) 394, 592
PstNI CAGNNNCTG 3 cut(s) 109, 239, 389
PvuII CAGCTG 3 cut(s) 106, 254, 275
RsaI GTAC 3 cut(s) 89, 309, 396
RsaNI GTAC 3 cut(s) 88, 308, 395
SaqAI TTAA 8 cut(s) 69, 333, 401, 528, 540, 594, 617, 663
SatI GCNGC 9 cut(s) 15, 104, 107, 240, 261, 273, 282, 390, 694
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 3 cut(s) 311, 509, 735
SfaNI GCATC 4 cut(s) 226, 247, 268, 664
SfcI CTRYAG 2 cut(s) 390, 588
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
SnaBI TACGTA 1 cut(s) 488
Sse9I AATT 2 cut(s) 216, 660
SsiI CCGC 2 cut(s) 123, 170
SspMI CTAG 2 cut(s) 54, 608
TaiI ACGT 2 cut(s) 490, 726
TasI AATT 2 cut(s) 216, 660
TatI WGTACW 2 cut(s) 87, 307
TfiI GAWTC 2 cut(s) 563, 735
Tru1I TTAA 8 cut(s) 69, 333, 401, 528, 540, 594, 617, 663
Tru9I TTAA 8 cut(s) 69, 333, 401, 528, 540, 594, 617, 663
TscAI CASTG 2 cut(s) 592, 690
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 9 cut(s) 14, 103, 106, 239, 260, 272, 281, 389, 693
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 7 cut(s) 17, 255, 405, 635, 640, 644, 668
TspGWI ACGGA 2 cut(s) 491, 527
TspRI CASTG 2 cut(s) 592, 690
VspI ATTAAT 2 cut(s) 333, 540
XceI RCATGY 1 cut(s) 36
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XspI CTAG 2 cut(s) 54, 608
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.