RchiOBHm_Chr4g0427021

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008906
Physical Location & Seq
Forward (+)
52308445 .. 52309262
818 bp
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UTR
Exon/CDS
Intron
PRQ39598

Sequence Viewer

Length: 603 bp
ATGCTTAATTTAGATGCCAATAAAAAGGAGAAGAAGTATGTCTTTTATAGGACGATTGTCGGTGGTTCGGCTGGTTTCGATCAGTTTCTTCCTCTTGAAGAATTTACTGACGCCTCCAACGGTTATCTAATTGATGACACTTGTGAATTTGGCGCGGAGGTCTTTGTTTCTAAACAAGTAAGAACAGGAAAAGGAGAGTGTCTAGTGTCTATGATCAAAGATGTCACCAAGTGCAAGCGTGCTTGGAGCATTTCCAACTTTTCACAGTTGGCTGATGAACGCTATACCTCAAATCCGTTCTTTTTGACAGACCACAAATATTGCGAATGGAATCTACGACTCTATCCCAATGGAAATAAGTGGGGAAATGGTAGTGGTCATATTTCTGTTTATGTGGAATTGGCCAATTTAAAATCTCTTCCTCCAGGCTCTAAAGTATTGGCAGAGTTTACCATACAAATCCTAGATCAAATCAATGGGAAGCATCATTCTACCAAAGGTAAACATTGGCTGTATCAATTACGAGGGTTTGTCTTTTTATTTCACCAGGTTTACTATATATATACTTCTCAATTTAACTTGTTCTCTCTATACCTGTTTTAG

Protein Analysis

200

Amino Acids

23.09

Weight (kDa)

8.56

Isoelectric Point (pI)

18.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 81 - 170 2.6e-17 MATH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 155
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 2 cut(s) 101, 146
AcyI GRCGYC 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 231
AgsI TTSAA 1 cut(s) 98
AjnI CCWGG 2 cut(s) 424, 546
AoxI GGCC 1 cut(s) 402
ApoI RAATTY 2 cut(s) 101, 146
AspLEI GCGC 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 217, 536
BalI TGGCCA 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 426, 548
BclI TGATCA 1 cut(s) 213
BfaI CTAG 2 cut(s) 203, 464
Bme1390I CCNGG 2 cut(s) 426, 548
BmrFI CCNGG 2 cut(s) 426, 548
BmsI GCATC 2 cut(s) 4, 493
BoxI GACNNNNGTC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 111
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
BseBI CCWGG 2 cut(s) 426, 548
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 404
BsnI GGCC 1 cut(s) 404
Bsp143I GATC 3 cut(s) 79, 213, 466
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 404
BspFNI CGCG 1 cut(s) 155
BssMI GATC 3 cut(s) 79, 213, 466
BssNI GRCGYC 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 426, 548
Bst4CI ACNGT 2 cut(s) 122, 267
Bst6I CTCTTC 1 cut(s) 423
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 236, 240
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 3 cut(s) 82, 216, 469
BstMBI GATC 3 cut(s) 79, 213, 466
BstNI CCWGG 2 cut(s) 426, 548
BstPAI GACNNNNGTC 1 cut(s) 56
BstSCI CCNGG 2 cut(s) 424, 546
BstUI CGCG 1 cut(s) 155
BsuRI GGCC 1 cut(s) 404
Cac8I GCNNGC 2 cut(s) 236, 240
CfoI GCGC 1 cut(s) 155
CseI GACGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 546
CspCI CAANNNNNGTGG 2 cut(s) 302, 337
CviJI RGCY 5 cut(s) 71, 272, 404, 429, 511
CviKI_1 RGCY 5 cut(s) 71, 272, 404, 429, 511
DpnI GATC 3 cut(s) 81, 215, 468
DpnII GATC 3 cut(s) 79, 213, 466
DraI TTTAAA 1 cut(s) 411
DraIII CACNNNGTG 1 cut(s) 231
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 424, 546
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FbaI TGATCA 1 cut(s) 213
FspBI CTAG 2 cut(s) 203, 464
GlaI GCGC 1 cut(s) 154
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 1 cut(s) 404
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 2 cut(s) 331, 339
HphI GGTGA 2 cut(s) 217, 536
Hpy166II GTNNAC 3 cut(s) 450, 503, 553
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 3 cut(s) 450, 503, 553
HpyCH4III ACNGT 2 cut(s) 122, 267
HpyCH4V TGCA 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 3 cut(s) 79, 213, 466
LmnI GCTCC 1 cut(s) 246
LpnPI CCDG 6 cut(s) 57, 171, 411, 438, 533, 560
LweI GCATC 2 cut(s) 4, 493
MabI ACCWGGT 1 cut(s) 546
MaeI CTAG 2 cut(s) 203, 464
MaeIII GTNAC 1 cut(s) 223
MalI GATC 3 cut(s) 81, 215, 468
MboI GATC 3 cut(s) 79, 213, 466
MboII GAAGA 4 cut(s) 43, 80, 110, 410
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 8 cut(s) 7, 101, 129, 146, 398, 406, 518, 572
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 1 cut(s) 333
MmeI TCCRAC 2 cut(s) 141, 279
MnlI CCTC 6 cut(s) 102, 124, 151, 298, 432, 518
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
MseI TTAA 3 cut(s) 6, 410, 576
Msp20I TGGCCA 1 cut(s) 404
MspR9I CCNGG 2 cut(s) 426, 548
MvaI CCWGG 2 cut(s) 426, 548
MvnI CGCG 1 cut(s) 155
NdeII GATC 3 cut(s) 79, 213, 466
NmuCI GTSAC 1 cut(s) 223
PfeI GAWTC 1 cut(s) 331
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PshAI GACNNNNGTC 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 424, 546
PspGI CCWGG 2 cut(s) 424, 546
SaqAI TTAA 3 cut(s) 6, 410, 576
Sau3AI GATC 3 cut(s) 79, 213, 466
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 2 cut(s) 426, 548
SetI ASST 5 cut(s) 162, 290, 502, 552, 597
SexAI ACCWGGT 1 cut(s) 546
SfaNI GCATC 2 cut(s) 4, 493
Sse9I AATT 8 cut(s) 7, 101, 129, 146, 398, 406, 518, 572
SsiI CCGC 1 cut(s) 155
SspI AATATT 1 cut(s) 320
SspMI CTAG 2 cut(s) 203, 464
StyD4I CCNGG 2 cut(s) 424, 546
TaaI ACNGT 2 cut(s) 122, 267
TaqI TCGA 1 cut(s) 78
TasI AATT 8 cut(s) 7, 101, 129, 146, 398, 406, 518, 572
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 3 cut(s) 6, 410, 576
Tru9I TTAA 3 cut(s) 6, 410, 576
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 291
TspGWI ACGGA 1 cut(s) 285
XapI RAATTY 2 cut(s) 101, 146
XspI CTAG 2 cut(s) 203, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.