RchiOBHm_Chr5g0003521

quinone oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008845
Physical Location & Seq
Forward (+)
2211494 .. 2216327
4834 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28483

Sequence Viewer

Length: 1197 bp
ATGCTCCGGAAACTCGGATGTGGTGGGAGGTCAAGCACTGAGTCGTCGTTGTCGTCATCTGGCCCAAAGAAGGAGCTGTTGATGCGCGCACTAGTTGCTACAAGTGAAGGTGGCGAAGATGAGGTGCTCCAAGTACAACTTGTGCCCAGACCAGTACCTAAAGCAAAAGAGGTACTGATCAAAGTAGCCTATGCCGGGGTCAACCGAGCGGACGTGGAAGAGACCAACCTCGGTGAGCCAACTCTTAACGAACTTGAGAAGGCCGGACTTAAAGGAAAATATATGGGAGTAGAATGTTCAGGAACAGTATGTGCTCATGGGGAGATGTCCAAAAAAGACAAGCAAACCTGGAAAATAGACACTAAGGTGTGTGCGATTCTTGTCGGTGGTGCCTATGCGGAGTACGTCGCTGCTCCAATAGGACACCTTCTGAAATTCACAACGAAGATTCCTGTCGAGCTAGCTGCTGCTATTCCACAAGATGCTTGCACCGTCTGGTCGGAATTGTTTATGAAGAAAAGGCTATACGAGGGGTCTGCACCGGAGGAGGAGGGAGAGGGGAGTCGGCTATCTTCCGGAGAAACAGTTTTGCTATTGGAAGTGCTTGAAGCATTGGGCTTTCCCTTTCTCTTTGATGTTGGTGGAGTGGCAGTGGATGCGGGCTGCAGCGGAATTGGCATATTTGCGATTCAGATGGCTAAGTACCAGGGAGCAACTGCATTTGCAACAGCAGGTACTAACGCAAAGATAGCTGAATGTAGACGTCTTGGAGCTGACCTATGCATCAACTATAACAAAAATGACTTTGTTGAAGTGATAAAGGACCGGACTAAAGGCCAAGGCGTTGATGTCATATTGGATTGTGTTGGGTGCAATGGTTTGCATAGAAACCTTGAATGCTTGAAGAAAGGTGGGAGACTTGTTCATATTTGTGACAATGGCCGTTGGATGAAAAAAATCGATGCTTATACAGAACTTTCAAATTACAAACTCATTGATGTGGCTTCCTTGTTATACAGAACTCCAGAGATGAAGACCGTCATTGTTAAGGAAGTTCAGGAGAAAGTGATGCCTGCAATTGAGAATGGCGACGTGAAACTTGTGATTCACAACCAGGTTTTTACATTCGAAGAAGCAGCCGAGGCGCACAAACTCAAGAAAAGCAATCTAATCGGAAAAATATTGCTTCACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

398

Amino Acids

43.13

Weight (kDa)

6.68

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 55 - 145 1.3e-07 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 224 - 310 1.2e-23 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 256 - 396 2.5e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 766
Acc36I ACCTGC 1 cut(s) 722
AccB1I GGYRCC 1 cut(s) 389
AccBSI CCGCTC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 760
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 2 cut(s) 6, 575
AciI CCGC 4 cut(s) 209, 398, 659, 669
AcoI YGGCCR 1 cut(s) 940
AcsI RAATTY 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 763
AfaI GTAC 6 cut(s) 135, 156, 174, 404, 704, 736
AfiI CCNNNNNNNGG 2 cut(s) 70, 195
AgsI TTSAA 5 cut(s) 608, 812, 896, 904, 981
AhlI ACTAGT 1 cut(s) 91
AjiI CACGTC 2 cut(s) 214, 1093
AjnI CCWGG 3 cut(s) 347, 705, 1113
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 5 cut(s) 76, 460, 464, 752, 773
AluI AGCT 5 cut(s) 76, 460, 464, 752, 773
Alw21I GWGCWC 2 cut(s) 129, 316
Alw26I GTCTC 2 cut(s) 215, 910
Aor13HI TCCGGA 2 cut(s) 6, 575
AoxI GGCC 4 cut(s) 61, 261, 835, 940
ApeKI GCWGC 6 cut(s) 410, 464, 467, 663, 666, 1136
ApoI RAATTY 1 cut(s) 434
AspLEI GCGC 3 cut(s) 87, 89, 1147
AspS9I GGNCC 2 cut(s) 62, 823
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 245, 1181
AsuII TTCGAA 1 cut(s) 1128
AsuNHI GCTAGC 1 cut(s) 460
AvaII GGWCC 1 cut(s) 823
BaeGI GKGCMC 1 cut(s) 147
BanI GGYRCC 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 1040
Bbv12I GWGCWC 2 cut(s) 129, 316
BbvI GCAGC 6 cut(s) 397, 451, 454, 650, 678, 1148
BccI CCATC 1 cut(s) 688
BceAI ACGGC 1 cut(s) 927
BcgI CGANNNNNNTGC 6 cut(s) 446, 480, 518, 552, 1153, 1187
BciT130I CCWGG 3 cut(s) 349, 707, 1115
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 2 cut(s) 215, 910
BcuI ACTAGT 1 cut(s) 91
BfaI CTAG 2 cut(s) 92, 461
BfmI CTRYAG 1 cut(s) 664
BfuAI ACCTGC 1 cut(s) 722
BisI GCNGC 6 cut(s) 411, 465, 468, 664, 667, 1137
BlsI GCNGC 6 cut(s) 412, 466, 469, 665, 668, 1138
Bme1390I CCNGG 4 cut(s) 196, 349, 707, 1115
Bme18I GGWCC 1 cut(s) 823
BmgBI CACGTC 2 cut(s) 214, 1093
BmgT120I GGNCC 2 cut(s) 62, 823
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 4 cut(s) 196, 349, 707, 1115
BmsI GCATC 6 cut(s) 72, 472, 646, 792, 952, 1059
BmtI GCTAGC 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 1040
BpmI CTGGAG 1 cut(s) 1008
Bpu14I TTCGAA 1 cut(s) 1128
BpuEI CTTGAG 2 cut(s) 275, 1139
BpuMI CCSGG 1 cut(s) 196
Bsa29I ATCGAT 1 cut(s) 960
BsaHI GRCGYC 1 cut(s) 763
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 5 cut(s) 195, 229, 706, 838, 1140
BsaWI WCCGGW 4 cut(s) 6, 541, 575, 825
BsaXI ACNNNNNCTCC 4 cut(s) 570, 600, 636, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 195
Bse1I ACTGG 1 cut(s) 152
Bse3DI GCAATG 1 cut(s) 880
BseAI TCCGGA 2 cut(s) 6, 575
BseBI CCWGG 3 cut(s) 349, 707, 1115
BseCI ATCGAT 1 cut(s) 960
BseDI CCNNGG 5 cut(s) 195, 229, 706, 838, 1140
BseGI GGATG 3 cut(s) 23, 661, 954
BseLI CCNNNNNNNGG 2 cut(s) 70, 195
BseMI GCAATG 1 cut(s) 880
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 152
BsePI GCGCGC 1 cut(s) 85
BseRI GAGGAG 2 cut(s) 560, 563
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 6 cut(s) 397, 451, 454, 650, 678, 1148
BsgI GTGCAG 1 cut(s) 522
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 4 cut(s) 63, 263, 837, 942
BshNI GGYRCC 1 cut(s) 389
BshVI ATCGAT 1 cut(s) 960
BsiHKAI GWGCWC 2 cut(s) 129, 316
BsiSI CCGG 6 cut(s) 7, 195, 264, 542, 576, 826
BslI CCNNNNNNNGG 2 cut(s) 70, 195
BsmAI GTCTC 2 cut(s) 215, 910
BsmI GAATGC 1 cut(s) 902
BsnI GGCC 4 cut(s) 63, 263, 837, 942
Bso31I GGTCTC 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 1128
Bsp1286I GDGCHC 3 cut(s) 129, 147, 316
Bsp13I TCCGGA 2 cut(s) 6, 575
Bsp143I GATC 1 cut(s) 177
BspACI CCGC 4 cut(s) 209, 398, 659, 669
BspANI GGCC 4 cut(s) 63, 263, 837, 942
BspCNI CTCAG 1 cut(s) 31
BspDI ATCGAT 1 cut(s) 960
BspEI TCCGGA 2 cut(s) 6, 575
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 391
BspMAI CTGCAG 1 cut(s) 668
BspMI ACCTGC 1 cut(s) 722
BspOI GCTAGC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 1128
BspT107I GGYRCC 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 880
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 5 cut(s) 195, 229, 706, 838, 1140
BssHII GCGCGC 1 cut(s) 85
BssMI GATC 1 cut(s) 177
BssNI GRCGYC 1 cut(s) 763
BssT1I CCWWGG 1 cut(s) 838
Bst2UI CCWGG 3 cut(s) 349, 707, 1115
Bst4CI ACNGT 4 cut(s) 307, 493, 586, 1039
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 1 cut(s) 763
BstAPI GCANNNNNTGC 2 cut(s) 95, 656
BstBI TTCGAA 1 cut(s) 1128
BstC8I GCNNGC 5 cut(s) 87, 462, 487, 661, 1074
BstDEI CTNAG 3 cut(s) 39, 363, 699
BstF5I GGATG 3 cut(s) 23, 661, 954
BstFNI CGCG 1 cut(s) 87
BstHHI GCGC 3 cut(s) 87, 89, 1147
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 2 cut(s) 215, 910
BstMBI GATC 1 cut(s) 177
BstMWI GCNNNNNNNGC 6 cut(s) 82, 95, 656, 675, 749, 1142
BstNI CCWGG 3 cut(s) 349, 707, 1115
BstSCI CCNGG 4 cut(s) 194, 347, 705, 1113
BstSFI CTRYAG 1 cut(s) 664
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 6 cut(s) 397, 451, 454, 650, 678, 1148
BstV2I GAAGAC 1 cut(s) 1040
Bsu15I ATCGAT 1 cut(s) 960
BsuRI GGCC 4 cut(s) 63, 263, 837, 942
BsuTUI ATCGAT 1 cut(s) 960
BtrI CACGTC 2 cut(s) 214, 1093
BtsCI GGATG 3 cut(s) 23, 661, 954
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 2 cut(s) 36, 657
BveI ACCTGC 1 cut(s) 722
Cac8I GCNNGC 5 cut(s) 87, 462, 487, 661, 1074
CfoI GCGC 3 cut(s) 87, 89, 1147
Cfr13I GGNCC 2 cut(s) 62, 823
ClaI ATCGAT 1 cut(s) 960
CsiI ACCWGGT 1 cut(s) 1113
Csp6I GTAC 6 cut(s) 134, 155, 173, 403, 703, 735
CviAII CATG 1 cut(s) 317
CviQI GTAC 6 cut(s) 134, 155, 173, 403, 703, 735
DdeI CTNAG 3 cut(s) 39, 363, 699
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 940
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 838
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 823
EcoRII CCWGG 3 cut(s) 347, 705, 1113
EcoT14I CCWWGG 1 cut(s) 838
EcoT22I ATGCAT 1 cut(s) 785
ErhI CCWWGG 1 cut(s) 838
FaeI CATG 1 cut(s) 320
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FatI CATG 1 cut(s) 316
FauI CCCGC 1 cut(s) 652
FbaI TGATCA 1 cut(s) 177
FblI GTMKAC 1 cut(s) 760
Fnu4HI GCNGC 6 cut(s) 411, 465, 468, 664, 667, 1137
FokI GGATG 3 cut(s) 30, 668, 961
Fsp4HI GCNGC 6 cut(s) 411, 465, 468, 664, 667, 1137
FspBI CTAG 2 cut(s) 92, 461
GlaI GCGC 3 cut(s) 86, 88, 1146
GluI GCNGC 6 cut(s) 411, 465, 468, 664, 667, 1137
GsuI CTGGAG 1 cut(s) 1008
HaeIII GGCC 4 cut(s) 63, 263, 837, 942
HapII CCGG 6 cut(s) 7, 195, 264, 542, 576, 826
HhaI GCGC 3 cut(s) 87, 89, 1147
Hin1I GRCGYC 1 cut(s) 763
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 3 cut(s) 85, 87, 1145
HinP1I GCGC 3 cut(s) 85, 87, 1145
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 6 cut(s) 41, 376, 448, 562, 688, 1105
HpaII CCGG 6 cut(s) 7, 195, 264, 542, 576, 826
HphI GGTGA 2 cut(s) 245, 1181
Hpy166II GTNNAC 2 cut(s) 202, 761
Hpy188I TCNGA 5 cut(s) 17, 432, 502, 693, 1175
Hpy188III TCNNGA 6 cut(s) 7, 300, 576, 1025, 1058, 1156
Hpy8I GTNNAC 2 cut(s) 202, 761
Hpy99I CGWCG 3 cut(s) 49, 410, 1094
HpyAV CCTTC 4 cut(s) 64, 101, 253, 437
HpyCH4III ACNGT 4 cut(s) 307, 493, 586, 1039
HpyCH4IV ACGT 4 cut(s) 213, 405, 763, 1092
HpyCH4V TGCA 9 cut(s) 489, 539, 666, 719, 725, 783, 873, 883, 1076
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 95, 656, 675, 749, 1142
HpyF3I CTNAG 3 cut(s) 39, 363, 699
HpySE526I ACGT 4 cut(s) 213, 405, 763, 1092
Hsp92I GRCGYC 1 cut(s) 763
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 3 cut(s) 85, 87, 1145
Kpn2I TCCGGA 2 cut(s) 6, 575
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 6 cut(s) 9, 73, 132, 418, 710, 770
Lsp1109I GCAGC 6 cut(s) 397, 451, 454, 650, 678, 1148
LweI GCATC 6 cut(s) 72, 472, 646, 792, 952, 1059
MabI ACCWGGT 1 cut(s) 1113
MaeI CTAG 2 cut(s) 92, 461
MaeII ACGT 4 cut(s) 213, 405, 763, 1092
MaeIII GTNAC 1 cut(s) 932
MalI GATC 1 cut(s) 179
MbiI CCGCTC 1 cut(s) 209
MboI GATC 1 cut(s) 177
MboII GAAGA 8 cut(s) 128, 230, 457, 526, 564, 916, 1045, 1142
MfeI CAATTG 1 cut(s) 1077
MhlI GDGCHC 3 cut(s) 129, 147, 316
MluCI AATT 5 cut(s) 434, 503, 672, 982, 1077
MlyI GAGTC 2 cut(s) 50, 571
MmeI TCCRAC 2 cut(s) 480, 926
Mph1103I ATGCAT 1 cut(s) 785
MroI TCCGGA 2 cut(s) 6, 575
MseI TTAA 3 cut(s) 246, 270, 1047
MslI CAYNNNNRTG 3 cut(s) 365, 930, 998
MspA1I CMGCKG 1 cut(s) 669
MspI CCGG 6 cut(s) 7, 195, 264, 542, 576, 826
MspR9I CCNGG 4 cut(s) 196, 349, 707, 1115
MunI CAATTG 1 cut(s) 1077
Mva1269I GAATGC 1 cut(s) 902
MvaI CCWGG 3 cut(s) 349, 707, 1115
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 6 cut(s) 82, 95, 656, 675, 749, 1142
NciI CCSGG 1 cut(s) 196
NdeII GATC 1 cut(s) 177
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 1 cut(s) 391
NmeAIII GCCGAG 1 cut(s) 1165
NmuCI GTSAC 1 cut(s) 932
NsiI ATGCAT 1 cut(s) 785
NspV TTCGAA 1 cut(s) 1128
OliI CACNNNNGTG 1 cut(s) 365
PauI GCGCGC 1 cut(s) 85
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PctI GAATGC 1 cut(s) 902
PfeI GAWTC 4 cut(s) 376, 448, 688, 1105
PkrI GCNGC 6 cut(s) 412, 466, 469, 665, 668, 1138
PleI GAGTC 2 cut(s) 49, 570
PpsI GAGTC 2 cut(s) 49, 570
Psp6I CCWGG 3 cut(s) 347, 705, 1113
PspGI CCWGG 3 cut(s) 347, 705, 1113
PspN4I GGNNCC 1 cut(s) 391
PspPI GGNCC 2 cut(s) 62, 823
PstI CTGCAG 1 cut(s) 668
PteI GCGCGC 1 cut(s) 85
RsaI GTAC 6 cut(s) 135, 156, 174, 404, 704, 736
RsaNI GTAC 6 cut(s) 134, 155, 173, 403, 703, 735
RseI CAYNNNNRTG 3 cut(s) 365, 930, 998
SaqAI TTAA 3 cut(s) 246, 270, 1047
SatI GCNGC 6 cut(s) 411, 465, 468, 664, 667, 1137
Sau3AI GATC 1 cut(s) 177
Sau96I GGNCC 2 cut(s) 62, 823
SchI GAGTC 2 cut(s) 50, 571
ScrFI CCNGG 4 cut(s) 196, 349, 707, 1115
SduI GDGCHC 3 cut(s) 129, 147, 316
SexAI ACCWGGT 1 cut(s) 1113
SfaNI GCATC 6 cut(s) 72, 472, 646, 792, 952, 1059
SfcI CTRYAG 1 cut(s) 664
SfuI TTCGAA 1 cut(s) 1128
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 3 cut(s) 365, 930, 998
SmlI CTYRAG 2 cut(s) 254, 1154
SmoI CTYRAG 2 cut(s) 254, 1154
SpeI ACTAGT 1 cut(s) 91
Sse9I AATT 5 cut(s) 434, 503, 672, 982, 1077
SsiI CCGC 4 cut(s) 209, 398, 659, 669
SspI AATATT 1 cut(s) 1182
SspMI CTAG 2 cut(s) 92, 461
StyD4I CCNGG 4 cut(s) 194, 347, 705, 1113
StyI CCWWGG 1 cut(s) 838
TaaI ACNGT 4 cut(s) 307, 493, 586, 1039
TaiI ACGT 4 cut(s) 216, 408, 766, 1095
TaqI TCGA 3 cut(s) 456, 960, 1128
TasI AATT 5 cut(s) 434, 503, 672, 982, 1077
TatI WGTACW 1 cut(s) 133
TfiI GAWTC 4 cut(s) 376, 448, 688, 1105
Tru1I TTAA 3 cut(s) 246, 270, 1047
Tru9I TTAA 3 cut(s) 246, 270, 1047
TscAI CASTG 2 cut(s) 43, 657
TseFI GTSAC 1 cut(s) 932
TseI GCWGC 6 cut(s) 410, 464, 467, 663, 666, 1136
Tsp45I GTSAC 1 cut(s) 932
TspDTI ATGAA 4 cut(s) 527, 914, 965, 1046
TspRI CASTG 2 cut(s) 43, 657
VpaK11BI GGWCC 1 cut(s) 823
XapI RAATTY 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 760
XspI CTAG 2 cut(s) 92, 461
ZraI GACGTC 1 cut(s) 764
Zsp2I ATGCAT 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.