Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0008845

Physical Location & Seq

Genomic Coordinates Reverse (-)

93550292 .. 93553638

Gene Structure 3347 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00072500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGCTCAAGAAGCTCGGAGGTGGTGGGAAGTCCCAGTCTTCCAAGAGAGGGAAAGAGAGTAAGAGCAATGAGAAAGATCAGAGTAAGAGTACTAGTACTGAGTCGACGTCGTCGGGACCACAGAAGGAGCTGTTGATGCGCGCCATAGTTGCTACGAGTGAAGGTCAACTCAAAGTACAACTGGTGCCTAGACCAGTACCCAAAGCAAACGAGGTACTTGTCAAAGTAGACTACGCCGGGGTCAACGGACCCGACGTTGAAGAGAGCAAGCACGGCAAGTCACCTCAGATCAAGGAACTTGAGGAGGATGGAACTAAAAGCAAGTATTTGGGTCTAGAGTGTTCAGGAACCATCTGTGCTCTTGGGGAGGGGATCTCCAAAAAAGACAAGCAAATCTGGAAAAAAGACACTAAGGTGTGTGCCATTGTTTTTGGCGGTGCCTATGCGGAGTACGTTGCTGTTCCAGTAGGACACCTTCTGAAATTCACAACAAAGATTAACGTCAAGCAAGCTGCTGCTATTCCACAAGCTGCTTGCACCGTCTGGTCCGAATTATTCATGAAGAAAAGGCTAGATGAGGGGCTTGCAGTACAGGAGGAAGAAGGGAGTCGGCTTTCTTCAGGCGAGACAGTTTTGCTCCAATGTATTGAGCCTAAGTTTACAGTCAAGGCTACTTTGTACGATGAGGATAATCTGAAAAGCTATCAGAAGCTATTGGAAGTGCTTGAAGCATTGGCCTTTCCCTTTCTCTTTGATGTTGGGTGCAGTGGCAGTGGATTCGGGTTGGACTTCATTTTGACTAATACTATATACATTCATGGAGGCTGCAGTGGAATTGGCATATTTGCGATTCAGATGGCTAAGTACCAGGGAGCCATTGTATTTGCAACAGCAGGTACCGACGCAAAGATAGCTGAATGTAAACGTCTTGGAGCTGACCTATGCATCAACTATAACAAAAATGACTTTGTTGAAGTGATAAGGGACAGCACTAAAGGCCAAGGCGTTGATGTTATCTTGGATTGCGTTGGGTTTAAGTATTTTCAGAGAAATCTTGAATGCCTGAAGGAAGGTGGGAGACTTGTTCAAATTGGTGGCTGTTGGATGACACAATACGATTCTTATGGAAAAGTACAAAAGCTCTTTTCAAATTTCAAATGCATTGATGTGGCTGCCTTGGTATACAGAACTCCAGAGATGAAGACCTCCATTGTTGAGGAGGTTCAGGGGAAAGTGATGCCTGCAATTGAGAATGGCAACGTGAAACTTCTGATTCACAAGGTTTTTACATTCGAAGAAGCAGCTGAGGCACACAAACTCAAGAAAAGTGGCAATCATATCGGAAAGATATTGCTTCACCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

454

Amino Acids

49.55

Weight (kDa)

8.5

Isoelectric Point (pI)

36.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	69 - 161	2.5e-09	Alcohol dehydrogenase GroES-like domain
ADH_zinc_N	PF00107	278 - 372	4e-28	Zinc-binding dehydrogenase
ADH_zinc_N_2	PF13602	310 - 452	1e-24	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008845)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g02170
rosa_chinensis	RchiOBHm_Chr5g0003051 RchiOBHm_Chr5g0003521
rosa_laevigata	RLG00000031095 RLG00000031097 RLG00000031110
rosa_multiflora	Rmu_co8296325.1_g000001 Rmu_sc0000146.1_g000013 Rmu_sc0004279.1_g000013
rosa_roxburghii	Rroxscaffold_1G00072500
rosa_rugosa	Rorug04G0401800 Rorug04G0402000 Rorug04G0402100
rosa_samantha	Rh5AG024500 Rh5BG027900 Rh5CG027400 Rh5CG033300 Rh5DG027900 Rh6DG004000
rosa_wichuraiana	Rw5G002530 Rw5G002550 Rw5G002620 Rw5G002880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	110
Acc36I	ACCTGC	1 cut(s)	884
Acc65I	GGTACC	1 cut(s)	896
AccB1I	GGYRCC	3 cut(s)	184, 437, 896
AccI	GTMKAC	3 cut(s)	104, 228, 1182
AccII	CGCG	1 cut(s)	141
AciI	CCGC	2 cut(s)	435, 446
AclWI	GGATC	1 cut(s)	380
AcsI	RAATTY	2 cut(s)	482, 1150
AcuI	CTGAAG	2 cut(s)	603, 1085
AcyI	GRCGYC	1 cut(s)	107
AfiI	CCNNNNNNNGG	1 cut(s)	48
AgsI	TTSAA	7 cut(s)	260, 728, 974, 1058, 1088, 1149, 1156
AhlI	ACTAGT	1 cut(s)	92
AjnI	CCWGG	1 cut(s)	867
AleI	CACNNNNGTG	1 cut(s)	413
Alw21I	GWGCWC	1 cut(s)	361
Alw26I	GTCTC	2 cut(s)	620, 1072
AlwI	GGATC	1 cut(s)	380
AoxI	GGCC	2 cut(s)	735, 997
ApeKI	GCWGC	6 cut(s)	512, 515, 530, 825, 1172, 1301
ApoI	RAATTY	2 cut(s)	482, 1150
Asp718I	GGTACC	1 cut(s)	896
AspLEI	GCGC	2 cut(s)	141, 143
AspS9I	GGNCC	3 cut(s)	116, 248, 546
AsuC2I	CCSGG	1 cut(s)	238
AsuHPI	GGTGA	2 cut(s)	273, 1349
AsuII	TTCGAA	1 cut(s)	1293
AvaII	GGWCC	3 cut(s)	116, 248, 546
BanI	GGYRCC	3 cut(s)	184, 437, 896
BarI	GAAGNNNNNNTAC	2 cut(s)	459, 491
BbsI	GAAGAC	2 cut(s)	30, 1208
Bbv12I	GWGCWC	1 cut(s)	361
BbvCI	CCTCAGC	1 cut(s)	1305
BbvI	GCAGC	6 cut(s)	499, 502, 517, 812, 1159, 1313
BccI	CCATC	3 cut(s)	302, 359, 850
BceAI	ACGGC	1 cut(s)	289
BcgI	CGANNNNNNTGC	4 cut(s)	760, 794, 1321, 1355
BciT130I	CCWGG	1 cut(s)	869
BcnI	CCSGG	1 cut(s)	238
BcoDI	GTCTC	2 cut(s)	620, 1072
BcuI	ACTAGT	1 cut(s)	92
BfaI	CTAG	4 cut(s)	93, 189, 335, 572
BfmI	CTRYAG	1 cut(s)	826
BfuAI	ACCTGC	1 cut(s)	884
BisI	GCNGC	6 cut(s)	513, 516, 531, 826, 1173, 1302
BlsI	GCNGC	6 cut(s)	514, 517, 532, 827, 1174, 1303
BmcAI	AGTACT	2 cut(s)	91, 97
Bme1390I	CCNGG	2 cut(s)	238, 869
Bme18I	GGWCC	3 cut(s)	116, 248, 546
BmgT120I	GGNCC	3 cut(s)	116, 248, 546
BmiI	GGNNCC	7 cut(s)	117, 186, 250, 349, 439, 874, 898
BmrFI	CCNGG	2 cut(s)	238, 869
BmrI	ACTGGG	1 cut(s)	28
BmsI	GCATC	3 cut(s)	126, 954, 1227
BmuI	ACTGGG	1 cut(s)	28
BpiI	GAAGAC	2 cut(s)	30, 1208
BplI	GAGNNNNNCTC	2 cut(s)	359, 391
BpmI	CTGGAG	1 cut(s)	1176
Bpu10I	CCTNAGC	1 cut(s)	1305
Bpu14I	TTCGAA	1 cut(s)	1293
BpuEI	CTTGAG	2 cut(s)	320, 1304
BpuMI	CCSGG	1 cut(s)	238
BsaHI	GRCGYC	1 cut(s)	107
BsaJI	CCNNGG	4 cut(s)	237, 868, 1000, 1176
BsaXI	ACNNNNNCTCC	2 cut(s)	864, 894
Bsc4I	CCNNNNNNNGG	1 cut(s)	48
Bse1I	ACTGG	4 cut(s)	34, 186, 194, 464
Bse3DI	GCAATG	1 cut(s)	73
BseBI	CCWGG	1 cut(s)	869
BseDI	CCNNGG	4 cut(s)	237, 868, 1000, 1176
BseGI	GGATG	2 cut(s)	313, 1110
BseLI	CCNNNNNNNGG	1 cut(s)	48
BseMI	GCAATG	1 cut(s)	73
BseMII	CTCAG	3 cut(s)	90, 299, 1296
BseNI	ACTGG	4 cut(s)	34, 186, 194, 464
BsePI	GCGCGC	1 cut(s)	139
BseRI	GAGGAG	2 cut(s)	317, 1232
BseXI	GCAGC	6 cut(s)	499, 502, 517, 812, 1159, 1313
BsgI	GTGCAG	1 cut(s)	784
Bsh1236I	CGCG	1 cut(s)	141
BshFI	GGCC	2 cut(s)	737, 999
BshNI	GGYRCC	3 cut(s)	184, 437, 896
BsiHKAI	GWGCWC	1 cut(s)	361
BsiSI	CCGG	1 cut(s)	237
BslFI	GGGAC	3 cut(s)	16, 129, 998
BslI	CCNNNNNNNGG	1 cut(s)	48
BsmAI	GTCTC	2 cut(s)	620, 1072
BsmFI	GGGAC	3 cut(s)	16, 129, 998
BsmI	GAATGC	1 cut(s)	1064
BsnI	GGCC	2 cut(s)	737, 999
Bsp119I	TTCGAA	1 cut(s)	1293
Bsp1286I	GDGCHC	1 cut(s)	361
Bsp143I	GATC	3 cut(s)	76, 288, 372
BspACI	CCGC	2 cut(s)	435, 446
BspANI	GGCC	2 cut(s)	737, 999
BspCNI	CTCAG	3 cut(s)	91, 298, 1297
BspFNI	CGCG	1 cut(s)	141
BspHI	TCATGA	1 cut(s)	558
BspLI	GGNNCC	7 cut(s)	117, 186, 250, 349, 439, 874, 898
BspMAI	CTGCAG	1 cut(s)	830
BspMI	ACCTGC	1 cut(s)	884
BspPI	GGATC	1 cut(s)	380
BspT104I	TTCGAA	1 cut(s)	1293
BspT107I	GGYRCC	3 cut(s)	184, 437, 896
BsrDI	GCAATG	1 cut(s)	73
BsrI	ACTGG	4 cut(s)	34, 186, 194, 464
BssECI	CCNNGG	4 cut(s)	237, 868, 1000, 1176
BssHII	GCGCGC	1 cut(s)	139
BssMI	GATC	3 cut(s)	76, 288, 372
BssNAI	GTATAC	1 cut(s)	1183
BssNI	GRCGYC	1 cut(s)	107
BssT1I	CCWWGG	2 cut(s)	1000, 1176
Bst1107I	GTATAC	1 cut(s)	1183
Bst2UI	CCWGG	1 cut(s)	869
Bst4CI	ACNGT	3 cut(s)	541, 631, 664
Bst6I	CTCTTC	1 cut(s)	255
BstACI	GRCGYC	1 cut(s)	107
BstBI	TTCGAA	1 cut(s)	1293
BstC8I	GCNNGC	6 cut(s)	141, 269, 510, 535, 585, 1242
BstDEI	CTNAG	6 cut(s)	99, 285, 411, 654, 861, 1305
BstF5I	GGATG	2 cut(s)	313, 1110
BstFNI	CGCG	1 cut(s)	141
BstHHI	GCGC	2 cut(s)	141, 143
BstKTI	GATC	3 cut(s)	79, 291, 375
BstMAI	GTCTC	2 cut(s)	620, 1072
BstMBI	GATC	3 cut(s)	76, 288, 372
BstMWI	GCNNNNNNNGC	7 cut(s)	10, 136, 149, 273, 911, 996, 1307
BstNI	CCWGG	1 cut(s)	869
BstSCI	CCNGG	2 cut(s)	236, 867
BstSFI	CTRYAG	1 cut(s)	826
BstUI	CGCG	1 cut(s)	141
BstV1I	GCAGC	6 cut(s)	499, 502, 517, 812, 1159, 1313
BstV2I	GAAGAC	2 cut(s)	30, 1208
BstX2I	RGATCY	1 cut(s)	372
BstYI	RGATCY	1 cut(s)	372
BstZ17I	GTATAC	1 cut(s)	1183
BsuRI	GGCC	2 cut(s)	737, 999
BtsCI	GGATG	2 cut(s)	313, 1110
BtsI	GCAGTG	3 cut(s)	772, 778, 835
BtsIMutI	CAGTG	3 cut(s)	772, 778, 835
BveI	ACCTGC	1 cut(s)	884
Cac8I	GCNNGC	6 cut(s)	141, 269, 510, 535, 585, 1242
CciI	TCATGA	1 cut(s)	558
CfoI	GCGC	2 cut(s)	141, 143
Cfr13I	GGNCC	3 cut(s)	116, 248, 546
CseI	GACGC	1 cut(s)	911
CspCI	CAANNNNNGTGG	2 cut(s)	1309, 1344
CviAII	CATG	2 cut(s)	559, 818
DdeI	CTNAG	6 cut(s)	99, 285, 411, 654, 861, 1305
DpnI	GATC	3 cut(s)	78, 290, 374
DpnII	GATC	3 cut(s)	76, 288, 372
Eam1104I	CTCTTC	1 cut(s)	255
EarI	CTCTTC	1 cut(s)	255
Eco130I	CCWWGG	2 cut(s)	1000, 1176
Eco47I	GGWCC	3 cut(s)	116, 248, 546
Eco57I	CTGAAG	2 cut(s)	603, 1085
EcoRII	CCWGG	1 cut(s)	867
EcoT14I	CCWWGG	2 cut(s)	1000, 1176
EcoT22I	ATGCAT	2 cut(s)	947, 1163
ErhI	CCWWGG	2 cut(s)	1000, 1176
FaeI	CATG	2 cut(s)	562, 821
FaqI	GGGAC	3 cut(s)	16, 129, 998
FatI	CATG	2 cut(s)	558, 817
FblI	GTMKAC	3 cut(s)	104, 228, 1182
Fnu4HI	GCNGC	6 cut(s)	513, 516, 531, 826, 1173, 1302
FokI	GGATG	2 cut(s)	320, 1117
Fsp4HI	GCNGC	6 cut(s)	513, 516, 531, 826, 1173, 1302
FspBI	CTAG	4 cut(s)	93, 189, 335, 572
GlaI	GCGC	2 cut(s)	140, 142
GluI	GCNGC	6 cut(s)	513, 516, 531, 826, 1173, 1302
GsuI	CTGGAG	1 cut(s)	1176
HaeIII	GGCC	2 cut(s)	737, 999
HapII	CCGG	1 cut(s)	237
HgaI	GACGC	1 cut(s)	911
HhaI	GCGC	2 cut(s)	141, 143
Hin1I	GRCGYC	1 cut(s)	107
Hin1II	CATG	2 cut(s)	562, 821
Hin6I	GCGC	2 cut(s)	139, 141
HinP1I	GCGC	2 cut(s)	139, 141
HincII	GTYRAC	3 cut(s)	105, 167, 244
HindII	GTYRAC	3 cut(s)	105, 167, 244
HinfI	GANTC	6 cut(s)	101, 607, 777, 850, 1118, 1273
HpaII	CCGG	1 cut(s)	237
HphI	GGTGA	2 cut(s)	273, 1349
Hpy166II	GTNNAC	7 cut(s)	105, 167, 229, 244, 660, 923, 1183
Hpy188III	TCNNGA	9 cut(s)	7, 114, 335, 345, 397, 559, 1055, 1193, 1321
Hpy8I	GTNNAC	7 cut(s)	105, 167, 229, 244, 660, 923, 1183
Hpy99I	CGWCG	5 cut(s)	109, 112, 115, 257, 905
HpyAV	CCTTC	6 cut(s)	118, 155, 485, 596, 1060, 1064
HpyCH4III	ACNGT	3 cut(s)	541, 631, 664
HpyCH4IV	ACGT	6 cut(s)	107, 255, 453, 501, 925, 1260
HpyCH4V	TGCA	8 cut(s)	537, 587, 765, 828, 887, 945, 1161, 1244
HpyF10VI	GCNNNNNNNGC	7 cut(s)	10, 136, 149, 273, 911, 996, 1307
HpyF3I	CTNAG	6 cut(s)	99, 285, 411, 654, 861, 1305
HpySE526I	ACGT	6 cut(s)	107, 255, 453, 501, 925, 1260
Hsp92I	GRCGYC	1 cut(s)	107
Hsp92II	CATG	2 cut(s)	562, 821
HspAI	GCGC	2 cut(s)	139, 141
KpnI	GGTACC	1 cut(s)	900
Kzo9I	GATC	3 cut(s)	76, 288, 372
LmnI	GCTCC	4 cut(s)	127, 642, 872, 932
Lsp1109I	GCAGC	6 cut(s)	499, 502, 517, 812, 1159, 1313
LweI	GCATC	3 cut(s)	126, 954, 1227
MaeI	CTAG	4 cut(s)	93, 189, 335, 572
MaeII	ACGT	6 cut(s)	107, 255, 453, 501, 925, 1260
MaeIII	GTNAC	1 cut(s)	279
MalI	GATC	3 cut(s)	78, 290, 374
MboI	GATC	3 cut(s)	76, 288, 372
MboII	GAAGA	7 cut(s)	30, 272, 574, 609, 611, 1213, 1307
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	1 cut(s)	372
MhlI	GDGCHC	1 cut(s)	361
MluCI	AATT	6 cut(s)	482, 551, 834, 1089, 1150, 1245
MlyI	GAGTC	2 cut(s)	110, 616
MmeI	TCCRAC	2 cut(s)	765, 1082
Mph1103I	ATGCAT	2 cut(s)	947, 1163
MseI	TTAA	2 cut(s)	498, 1035
MslI	CAYNNNNRTG	2 cut(s)	413, 1166
MspA1I	CMGCKG	1 cut(s)	1304
MspI	CCGG	1 cut(s)	237
MspR9I	CCNGG	2 cut(s)	238, 869
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	1 cut(s)	1064
MvaI	CCWGG	1 cut(s)	869
MvnI	CGCG	1 cut(s)	141
MwoI	GCNNNNNNNGC	7 cut(s)	10, 136, 149, 273, 911, 996, 1307
NciI	CCSGG	1 cut(s)	238
NdeII	GATC	3 cut(s)	76, 288, 372
NlaIII	CATG	2 cut(s)	562, 821
NlaIV	GGNNCC	7 cut(s)	117, 186, 250, 349, 439, 874, 898
NmuCI	GTSAC	1 cut(s)	279
NsiI	ATGCAT	2 cut(s)	947, 1163
NspV	TTCGAA	1 cut(s)	1293
OliI	CACNNNNGTG	1 cut(s)	413
PagI	TCATGA	1 cut(s)	558
PauI	GCGCGC	1 cut(s)	139
PcsI	WCGNNNNNNNCGW	1 cut(s)	252
PctI	GAATGC	1 cut(s)	1064
PfeI	GAWTC	4 cut(s)	777, 850, 1118, 1273
PflFI	GACNNNGTC	1 cut(s)	109
PkrI	GCNGC	6 cut(s)	514, 517, 532, 827, 1174, 1303
PleI	GAGTC	2 cut(s)	109, 615
PpsI	GAGTC	2 cut(s)	109, 615
Psp6I	CCWGG	1 cut(s)	867
PspGI	CCWGG	1 cut(s)	867
PspN4I	GGNNCC	7 cut(s)	117, 186, 250, 349, 439, 874, 898
PspPI	GGNCC	3 cut(s)	116, 248, 546
PstI	CTGCAG	1 cut(s)	830
PsuI	RGATCY	1 cut(s)	372
PsyI	GACNNNGTC	1 cut(s)	109
PteI	GCGCGC	1 cut(s)	139
PvuII	CAGCTG	1 cut(s)	1304
RseI	CAYNNNNRTG	2 cut(s)	413, 1166
SalI	GTCGAC	1 cut(s)	103
SaqAI	TTAA	2 cut(s)	498, 1035
SatI	GCNGC	6 cut(s)	513, 516, 531, 826, 1173, 1302
Sau3AI	GATC	3 cut(s)	76, 288, 372
Sau96I	GGNCC	3 cut(s)	116, 248, 546
ScaI	AGTACT	2 cut(s)	91, 97
SchI	GAGTC	2 cut(s)	110, 616
ScrFI	CCNGG	2 cut(s)	238, 869
SduI	GDGCHC	1 cut(s)	361
SfaNI	GCATC	3 cut(s)	126, 954, 1227
SfcI	CTRYAG	1 cut(s)	826
SfuI	TTCGAA	1 cut(s)	1293
SinI	GGWCC	3 cut(s)	116, 248, 546
SmiMI	CAYNNNNRTG	2 cut(s)	413, 1166
SmlI	CTYRAG	3 cut(s)	5, 299, 1319
SmoI	CTYRAG	3 cut(s)	5, 299, 1319
SpeI	ACTAGT	1 cut(s)	92
Sse9I	AATT	6 cut(s)	482, 551, 834, 1089, 1150, 1245
SsiI	CCGC	2 cut(s)	435, 446
SspMI	CTAG	4 cut(s)	93, 189, 335, 572
StyD4I	CCNGG	2 cut(s)	236, 867
StyI	CCWWGG	2 cut(s)	1000, 1176
TaaI	ACNGT	3 cut(s)	541, 631, 664
TaiI	ACGT	6 cut(s)	110, 258, 456, 504, 928, 1263
TaqI	TCGA	2 cut(s)	104, 1293
TasI	AATT	6 cut(s)	482, 551, 834, 1089, 1150, 1245
TatI	WGTACW	5 cut(s)	89, 95, 175, 589, 1132
TfiI	GAWTC	4 cut(s)	777, 850, 1118, 1273
Tru1I	TTAA	2 cut(s)	498, 1035
Tru9I	TTAA	2 cut(s)	498, 1035
TscAI	CASTG	3 cut(s)	772, 778, 835
TseFI	GTSAC	1 cut(s)	279
TseI	GCWGC	6 cut(s)	512, 515, 530, 825, 1172, 1301
Tsp45I	GTSAC	1 cut(s)	279
TspDTI	ATGAA	5 cut(s)	547, 575, 781, 806, 1214
TspGWI	ACGGA	1 cut(s)	261
TspRI	CASTG	3 cut(s)	772, 778, 835
Tth111I	GACNNNGTC	1 cut(s)	109
VpaK11BI	GGWCC	3 cut(s)	116, 248, 546
XapI	RAATTY	2 cut(s)	482, 1150
XbaI	TCTAGA	1 cut(s)	334
XmiI	GTMKAC	3 cut(s)	104, 228, 1182
XspI	CTAG	4 cut(s)	93, 189, 335, 572
ZraI	GACGTC	1 cut(s)	108
ZrmI	AGTACT	2 cut(s)	91, 97
Zsp2I	ATGCAT	2 cut(s)	947, 1163

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.