Rh5DG027900

quinone oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008845
Physical Location & Seq
Reverse (-)
2000255 .. 2011272
11018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG027900.1

Sequence Viewer

Length: 651 bp
ATGCTCAAGAAGCTCGGAGGTGGTGGGAAGTCCCAGTCTTCCAAGAGAGGGAAAGAGAGTAAGAGCAATGAGAAAGATCAGAGTAAGAGTACTAGTACTGAGTCGACGTCGTCGGGACCACGGAAGGAGCTGTTGATGCGCGCCATAGTTGCTACGAGTGAAGGTCAACTCAAAGTACAACTGGTGCCTAGACCAGTACCCAAAGCAAACGAGGTACTTGTCAAAGTAGGCTACGCCGGGGTCAACGGACCCGACGTTGACGTTGAAGAGAGCAAGCACGGCAAGTCACCTCAGATCAAGGAACTTGAGGAGGATGGAACTAAAAGCAAGTATTTGGGTCTAGAGTGTTCAGGAACCATCAGTGCTCTTGGGGAGGGGATCTCCAAAAAAGACAAGCAAATCTGGAAAAAAGACACTAAGGTGTGTGCCATTGTTTTTGGCGGTGCCTATGCGGAGTACGTTGCTGTTCCAGTAGGACACCTTCTGAAATTCACAACAAAGATTACCGTCAAGCAAGCTGCTGCTATTCCACAAGCCAGGGGCGGACCTGTTATCAGGCCCGACGGGGTCCGGACCCCCCCTACCTGTTTCACTTTGCTCTTAATTATGCTTGAGCTTTATAATGAAAACAGCAAAAGTTGGGTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

216

Amino Acids

23.2

Weight (kDa)

9.45

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 69 - 163 1.1e-09 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AatII GACGTC 1 cut(s) 110
AccB1I GGYRCC 2 cut(s) 184, 443
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 141
AccIII TCCGGA 1 cut(s) 570
AciI CCGC 3 cut(s) 441, 452, 543
AclWI GGATC 1 cut(s) 386
AcsI RAATTY 1 cut(s) 488
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 6 cut(s) 91, 97, 177, 198, 216, 458
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 1 cut(s) 266
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 1 cut(s) 536
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 4 cut(s) 13, 130, 518, 616
AluI AGCT 4 cut(s) 13, 130, 518, 616
Alw21I GWGCWC 1 cut(s) 367
AlwI GGATC 1 cut(s) 386
Aor13HI TCCGGA 1 cut(s) 570
AoxI GGCC 1 cut(s) 557
ApeKI GCWGC 2 cut(s) 518, 521
ApoI RAATTY 1 cut(s) 488
AspLEI GCGC 2 cut(s) 141, 143
AspS9I GGNCC 6 cut(s) 116, 248, 545, 558, 568, 573
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 1 cut(s) 279
AvaII GGWCC 5 cut(s) 116, 248, 545, 568, 573
BanI GGYRCC 2 cut(s) 184, 443
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 2 cut(s) 505, 508
BccI CCATC 2 cut(s) 308, 365
BceAI ACGGC 1 cut(s) 295
BciT130I CCWGG 1 cut(s) 538
BcnI CCSGG 1 cut(s) 238
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 3 cut(s) 93, 189, 341
BisI GCNGC 2 cut(s) 519, 522
BlsI GCNGC 2 cut(s) 520, 523
BmcAI AGTACT 2 cut(s) 91, 97
Bme1390I CCNGG 2 cut(s) 238, 538
Bme18I GGWCC 5 cut(s) 116, 248, 545, 568, 573
BmgT120I GGNCC 6 cut(s) 116, 248, 545, 558, 568, 573
BmiI GGNNCC 7 cut(s) 117, 186, 250, 355, 445, 569, 575
BmrFI CCNGG 2 cut(s) 238, 538
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 365, 397
BpuEI CTTGAG 2 cut(s) 326, 632
BpuMI CCSGG 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 119, 237, 537
BsaWI WCCGGW 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 4 cut(s) 34, 186, 194, 470
Bse3DI GCAATG 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 570
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 3 cut(s) 119, 237, 537
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 2 cut(s) 90, 305
BseNI ACTGG 4 cut(s) 34, 186, 194, 470
BsePI GCGCGC 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 2 cut(s) 505, 508
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 1 cut(s) 559
BshNI GGYRCC 2 cut(s) 184, 443
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 2 cut(s) 237, 571
BslFI GGGAC 2 cut(s) 16, 129
BslI CCNNNNNNNGG 1 cut(s) 48
BsmFI GGGAC 2 cut(s) 16, 129
BsnI GGCC 1 cut(s) 559
Bsp1286I GDGCHC 1 cut(s) 367
Bsp13I TCCGGA 1 cut(s) 570
Bsp143I GATC 3 cut(s) 76, 294, 378
BspACI CCGC 3 cut(s) 441, 452, 543
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 2 cut(s) 91, 304
BspEI TCCGGA 1 cut(s) 570
BspFNI CGCG 1 cut(s) 141
BspLI GGNNCC 7 cut(s) 117, 186, 250, 355, 445, 569, 575
BspPI GGATC 1 cut(s) 386
BspT107I GGYRCC 2 cut(s) 184, 443
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 4 cut(s) 34, 186, 194, 470
BssECI CCNNGG 3 cut(s) 119, 237, 537
BssHII GCGCGC 1 cut(s) 139
BssMI GATC 3 cut(s) 76, 294, 378
BssNI GRCGYC 1 cut(s) 107
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 261
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 141, 275, 516
BstDEI CTNAG 3 cut(s) 99, 291, 417
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 319
BstFNI CGCG 1 cut(s) 141
BstHHI GCGC 2 cut(s) 141, 143
BstKTI GATC 3 cut(s) 79, 297, 381
BstMBI GATC 3 cut(s) 76, 294, 378
BstMWI GCNNNNNNNGC 4 cut(s) 10, 136, 149, 279
BstNI CCWGG 1 cut(s) 538
BstSCI CCNGG 2 cut(s) 236, 536
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 2 cut(s) 505, 508
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 378
BstYI RGATCY 1 cut(s) 378
BsuRI GGCC 1 cut(s) 559
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 367
Cac8I GCNNGC 3 cut(s) 141, 275, 516
CfoI GCGC 2 cut(s) 141, 143
Cfr13I GGNCC 6 cut(s) 116, 248, 545, 558, 568, 573
Csp6I GTAC 6 cut(s) 90, 96, 176, 197, 215, 457
CviJI RGCY 7 cut(s) 13, 130, 231, 518, 536, 559, 616
CviKI_1 RGCY 7 cut(s) 13, 130, 231, 518, 536, 559, 616
CviQI GTAC 6 cut(s) 90, 96, 176, 197, 215, 457
DdeI CTNAG 3 cut(s) 99, 291, 417
DpnI GATC 3 cut(s) 78, 296, 380
DpnII GATC 3 cut(s) 76, 294, 378
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
EciI GGCGGA 1 cut(s) 558
Eco47I GGWCC 5 cut(s) 116, 248, 545, 568, 573
EcoRII CCWGG 1 cut(s) 536
FaiI YATR 4 cut(s) 146, 450, 608, 621
FaqI GGGAC 2 cut(s) 16, 129
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 2 cut(s) 519, 522
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 2 cut(s) 519, 522
FspBI CTAG 3 cut(s) 93, 189, 341
GlaI GCGC 2 cut(s) 140, 142
GluI GCNGC 2 cut(s) 519, 522
HaeIII GGCC 1 cut(s) 559
HapII CCGG 2 cut(s) 237, 571
HhaI GCGC 2 cut(s) 141, 143
Hin1I GRCGYC 1 cut(s) 107
Hin6I GCGC 2 cut(s) 139, 141
HinP1I GCGC 2 cut(s) 139, 141
HincII GTYRAC 4 cut(s) 105, 167, 244, 259
HindII GTYRAC 4 cut(s) 105, 167, 244, 259
HinfI GANTC 1 cut(s) 101
HpaII CCGG 2 cut(s) 237, 571
HphI GGTGA 1 cut(s) 279
Hpy166II GTNNAC 4 cut(s) 105, 167, 244, 259
Hpy188I TCNGA 4 cut(s) 17, 81, 294, 486
Hpy188III TCNNGA 6 cut(s) 7, 114, 341, 351, 403, 571
Hpy8I GTNNAC 4 cut(s) 105, 167, 244, 259
Hpy99I CGWCG 5 cut(s) 109, 112, 115, 257, 566
HpyAV CCTTC 3 cut(s) 118, 155, 491
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 4 cut(s) 107, 255, 261, 459
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 136, 149, 279
HpyF3I CTNAG 3 cut(s) 99, 291, 417
HpySE526I ACGT 4 cut(s) 107, 255, 261, 459
Hsp92I GRCGYC 1 cut(s) 107
HspAI GCGC 2 cut(s) 139, 141
Kpn2I TCCGGA 1 cut(s) 570
Kzo9I GATC 3 cut(s) 76, 294, 378
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 2 cut(s) 505, 508
LweI GCATC 1 cut(s) 126
MaeI CTAG 3 cut(s) 93, 189, 341
MaeII ACGT 4 cut(s) 107, 255, 261, 459
MaeIII GTNAC 1 cut(s) 285
MalI GATC 3 cut(s) 78, 296, 380
MboI GATC 3 cut(s) 76, 294, 378
MboII GAAGA 2 cut(s) 30, 278
MflI RGATCY 1 cut(s) 378
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 2 cut(s) 488, 603
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 7 cut(s) 11, 41, 205, 300, 301, 304, 367
MroI TCCGGA 1 cut(s) 570
MseI TTAA 1 cut(s) 602
MslI CAYNNNNRTG 1 cut(s) 419
MspI CCGG 2 cut(s) 237, 571
MspR9I CCNGG 2 cut(s) 238, 538
MvaI CCWGG 1 cut(s) 538
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 4 cut(s) 10, 136, 149, 279
NciI CCSGG 1 cut(s) 238
NdeII GATC 3 cut(s) 76, 294, 378
NlaIV GGNNCC 7 cut(s) 117, 186, 250, 355, 445, 569, 575
NmuCI GTSAC 1 cut(s) 285
OliI CACNNNNGTG 1 cut(s) 419
PauI GCGCGC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PflFI GACNNNGTC 2 cut(s) 109, 566
PkrI GCNGC 2 cut(s) 520, 523
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PsiI TTATAA 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 536
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 7 cut(s) 117, 186, 250, 355, 445, 569, 575
PspPI GGNCC 6 cut(s) 116, 248, 545, 558, 568, 573
PsuI RGATCY 1 cut(s) 378
PsyI GACNNNGTC 2 cut(s) 109, 566
PteI GCGCGC 1 cut(s) 139
RsaI GTAC 6 cut(s) 91, 97, 177, 198, 216, 458
RsaNI GTAC 6 cut(s) 90, 96, 176, 197, 215, 457
RseI CAYNNNNRTG 1 cut(s) 419
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 2 cut(s) 519, 522
Sau3AI GATC 3 cut(s) 76, 294, 378
Sau96I GGNCC 6 cut(s) 116, 248, 545, 558, 568, 573
ScaI AGTACT 2 cut(s) 91, 97
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 238, 538
SduI GDGCHC 1 cut(s) 367
SfaNI GCATC 1 cut(s) 126
SinI GGWCC 5 cut(s) 116, 248, 545, 568, 573
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 3 cut(s) 5, 305, 611
SmoI CTYRAG 3 cut(s) 5, 305, 611
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 2 cut(s) 488, 603
SsiI CCGC 3 cut(s) 441, 452, 543
SspMI CTAG 3 cut(s) 93, 189, 341
StyD4I CCNGG 2 cut(s) 236, 536
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 4 cut(s) 110, 258, 264, 462
TaqI TCGA 1 cut(s) 104
TasI AATT 2 cut(s) 488, 603
TatI WGTACW 3 cut(s) 89, 95, 175
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 285
TseI GCWGC 2 cut(s) 518, 521
Tsp45I GTSAC 1 cut(s) 285
TspDTI ATGAA 1 cut(s) 639
TspGWI ACGGA 2 cut(s) 136, 261
TspRI CASTG 1 cut(s) 367
Tth111I GACNNNGTC 2 cut(s) 109, 566
VpaK11BI GGWCC 5 cut(s) 116, 248, 545, 568, 573
XapI RAATTY 1 cut(s) 488
XbaI TCTAGA 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 104
XspI CTAG 3 cut(s) 93, 189, 341
ZraI GACGTC 1 cut(s) 108
ZrmI AGTACT 2 cut(s) 91, 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.