RchiOBHm_Chr5g0010591

Uncharacterised protein family UPF0052

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013634
Physical Location & Seq
Reverse (-)
6994007 .. 6996307
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29132

Sequence Viewer

Length: 414 bp
ATGAAATTTCTCATCCAACCCAGGGATATATGTCACTTGTCAATAAGCGAAACTTGGTATCGAGAACTTGATTGTTTTGGTGTCAGTGCTGTGGGGGCATGGTCCTCTGACCTCTGTGGTGGGAAGCTCTTCAGCTCCTGGCCTCCTGCACTCTCTTCGAGGATAAAGAGGATGTTCTACATGTCAAATGAGGGCCAAAACTTGCTTCTTGAGGTCTTCCCTTCTCCAAACGCAGCTGTGATGGATCAGCTACGTAATGTGGATTGCATTGTTTATGCCATGGGTTCCCTCTTTACTTCTGTTTGTCCATCACTGGTATTAATTGGCATTGGGGAGATTATATCTTCAAGGTCCTGCCCCAAGGTACCTAGTCATCACACTTTTAATTTACACATGTTACAAGATGCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0052

Protein Analysis

137

Amino Acids

15.23

Weight (kDa)

6.39

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CofD PF01933 53 - 122 3.2e-09 2-phospho-L-lactate transferase CofD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 364
AclWI GGATC 1 cut(s) 252
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 1 cut(s) 115
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 1 cut(s) 22
AflIII ACRYGT 2 cut(s) 180, 393
AgsI TTSAA 1 cut(s) 348
AjnI CCWGG 2 cut(s) 20, 137
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 4 cut(s) 127, 135, 236, 250
AluI AGCT 4 cut(s) 127, 135, 236, 250
AlwI GGATC 1 cut(s) 252
AlwNI CAGNNNCTG 1 cut(s) 138
AoxI GGCC 2 cut(s) 140, 193
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 5
AseI ATTAAT 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 128
Asp718I GGTACC 1 cut(s) 364
AspS9I GGNCC 3 cut(s) 102, 193, 351
AvaII GGWCC 2 cut(s) 102, 351
BanI GGYRCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 208
BbvI GCAGC 1 cut(s) 245
BccI CCATC 2 cut(s) 235, 316
BcgI CGANNNNNNTGC 2 cut(s) 138, 172
BciT130I CCWGG 2 cut(s) 22, 139
BfaI CTAG 1 cut(s) 369
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 2 cut(s) 22, 139
Bme18I GGWCC 2 cut(s) 102, 351
BmgT120I GGNCC 3 cut(s) 102, 193, 351
BmiI GGNNCC 2 cut(s) 286, 366
BmrFI CCNGG 2 cut(s) 22, 139
BmsI GCATC 1 cut(s) 394
BpiI GAAGAC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 254
BsaJI CCNNGG 4 cut(s) 20, 21, 279, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 318
BseBI CCWGG 2 cut(s) 22, 139
BseDI CCNNGG 4 cut(s) 20, 21, 279, 360
BseGI GGATG 2 cut(s) 12, 177
BseLI CCNNNNNNNGG 1 cut(s) 22
BseNI ACTGG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 2 cut(s) 142, 195
BshNI GGYRCC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 22
BsnI GGCC 2 cut(s) 142, 195
Bsp143I GATC 1 cut(s) 244
Bsp19I CCATGG 1 cut(s) 279
BspANI GGCC 2 cut(s) 142, 195
BspLI GGNNCC 2 cut(s) 286, 366
BspPI GGATC 1 cut(s) 252
BspQI GCTCTTC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 364
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 4 cut(s) 20, 21, 279, 360
BssMI GATC 1 cut(s) 244
BssT1I CCWWGG 2 cut(s) 279, 360
Bst2UI CCWGG 2 cut(s) 22, 139
Bst6I CTCTTC 2 cut(s) 134, 160
BstBAI YACGTR 1 cut(s) 254
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 2 cut(s) 12, 177
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstNI CCWGG 2 cut(s) 22, 139
BstNSI RCATGY 2 cut(s) 184, 397
BstSCI CCNGG 2 cut(s) 20, 137
BstSNI TACGTA 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 245
BstV2I GAAGAC 1 cut(s) 208
BsuRI GGCC 2 cut(s) 142, 195
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 2 cut(s) 12, 177
BtsIMutI CAGTG 2 cut(s) 91, 311
CaiI CAGNNNCTG 1 cut(s) 138
Cfr13I GGNCC 3 cut(s) 102, 193, 351
Csp6I GTAC 1 cut(s) 365
CviAII CATG 4 cut(s) 99, 181, 280, 394
CviJI RGCY 6 cut(s) 127, 135, 142, 195, 236, 250
CviKI_1 RGCY 6 cut(s) 127, 135, 142, 195, 236, 250
CviQI GTAC 1 cut(s) 365
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 134, 160
EarI CTCTTC 2 cut(s) 134, 160
Eco105I TACGTA 1 cut(s) 254
Eco130I CCWWGG 2 cut(s) 279, 360
Eco47I GGWCC 2 cut(s) 102, 351
Eco57I CTGAAG 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 20, 137
EcoT14I CCWWGG 2 cut(s) 279, 360
ErhI CCWWGG 2 cut(s) 279, 360
FaeI CATG 4 cut(s) 102, 184, 283, 397
FaiI YATR 9 cut(s) 29, 31, 100, 182, 276, 281, 341, 395, 412
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FatI CATG 4 cut(s) 98, 180, 279, 393
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 1 cut(s) 369
GluI GCNGC 1 cut(s) 234
HaeIII GGCC 2 cut(s) 142, 195
Hin1II CATG 4 cut(s) 102, 184, 283, 397
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 2 cut(s) 62, 209
HpyAV CCTTC 1 cut(s) 231
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 3 cut(s) 149, 267, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 4 cut(s) 102, 184, 283, 397
KpnI GGTACC 1 cut(s) 368
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 7 cut(s) 7, 34, 124, 151, 159, 299, 367
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 1 cut(s) 394
MaeI CTAG 1 cut(s) 369
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 2 cut(s) 32, 396
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 4 cut(s) 121, 147, 208, 336
MluCI AATT 3 cut(s) 5, 321, 385
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 8 cut(s) 115, 122, 153, 153, 162, 184, 205, 299
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 2 cut(s) 320, 384
MspA1I CMGCKG 1 cut(s) 236
MspR9I CCNGG 2 cut(s) 22, 139
MvaI CCWGG 2 cut(s) 22, 139
MwoI GCNNNNNNNGC 1 cut(s) 95
NcoI CCATGG 1 cut(s) 279
NdeII GATC 1 cut(s) 244
NlaIII CATG 4 cut(s) 102, 184, 283, 397
NlaIV GGNNCC 2 cut(s) 286, 366
NmuCI GTSAC 1 cut(s) 32
NspI RCATGY 2 cut(s) 184, 397
PasI CCCWGGG 1 cut(s) 21
PciI ACATGT 2 cut(s) 180, 393
PciSI GCTCTTC 1 cut(s) 134
PdmI GAANNNNTTC 1 cut(s) 128
PkrI GCNGC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 351
PscI ACATGT 2 cut(s) 180, 393
PshBI ATTAAT 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 20, 137
PspGI CCWGG 2 cut(s) 20, 137
PspN4I GGNNCC 2 cut(s) 286, 366
PspPI GGNCC 3 cut(s) 102, 193, 351
PspPPI RGGWCCY 1 cut(s) 351
PstNI CAGNNNCTG 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
SapI GCTCTTC 1 cut(s) 134
SaqAI TTAA 2 cut(s) 320, 384
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 3 cut(s) 102, 193, 351
ScrFI CCNGG 2 cut(s) 22, 139
SfaNI GCATC 1 cut(s) 394
SinI GGWCC 2 cut(s) 102, 351
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SnaBI TACGTA 1 cut(s) 254
Sse9I AATT 3 cut(s) 5, 321, 385
SspMI CTAG 1 cut(s) 369
StyD4I CCNGG 2 cut(s) 20, 137
StyI CCWWGG 2 cut(s) 279, 360
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 61, 158
TasI AATT 3 cut(s) 5, 321, 385
Tru1I TTAA 2 cut(s) 320, 384
Tru9I TTAA 2 cut(s) 320, 384
TscAI CASTG 2 cut(s) 91, 318
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 91, 318
VpaK11BI GGWCC 2 cut(s) 102, 351
VspI ATTAAT 1 cut(s) 320
XapI RAATTY 1 cut(s) 5
XceI RCATGY 2 cut(s) 184, 397
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.