Rh5DG078000

Uncharacterised protein family UPF0052

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013634
Physical Location & Seq
Reverse (-)
6847484 .. 6848755
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG078000.1

Sequence Viewer

Length: 336 bp
ATGTTGAGAATGGGGAAGACCTCAAGGGAAGCTCTTCAGCTCCTGGCCTTCTGCACTCTCTTCGAGGATAAGAGGATGTTCTACATGTCAAATGAGGGCCAAAACTTGCTTCTTGAGGTCTTCCCTTCTCCAAACGCAGCTGTGATGGATCAGCTACGTAATGTGGATTGCATTGTTTATGCCATGGGTTCCCTCTTTACTTCTGTTTGTCCATCACTGGTATTAATTGGCATTGGGGAGATTATATCTTCAAGGTCCTGCCCCAAGCCCCGTCACTATATCAATAGCCTATTAGTGCCCAAAGATGGTGAGATACTGTTAATGCTCAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0052

Protein Analysis

111

Amino Acids

12.4

Weight (kDa)

6.54

Isoelectric Point (pI)

67.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CofD PF01933 43 - 88 2.5e-08 2-phospho-L-lactate transferase CofD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 156
AcuI CTGAAG 1 cut(s) 20
AfiI CCNNNNNNNGG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 1 cut(s) 252
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
AluBI AGCT 4 cut(s) 32, 40, 140, 154
AluI AGCT 4 cut(s) 32, 40, 140, 154
AlwI GGATC 1 cut(s) 156
AlwNI CAGNNNCTG 1 cut(s) 43
AoxI GGCC 2 cut(s) 45, 97
ApeKI GCWGC 1 cut(s) 137
AseI ATTAAT 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 97, 255
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 300
BbsI GAAGAC 2 cut(s) 23, 112
BbvI GCAGC 1 cut(s) 149
BccI CCATC 3 cut(s) 139, 220, 299
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 1 cut(s) 44
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 97, 255
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 44
BpiI GAAGAC 2 cut(s) 23, 112
BpuEI CTTGAG 2 cut(s) 7, 134
BsaAI YACGTR 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 183
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 222
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 305
BseNI ACTGG 1 cut(s) 222
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 37
BshFI GGCC 2 cut(s) 47, 99
BslI CCNNNNNNNGG 1 cut(s) 305
BsnI GGCC 2 cut(s) 47, 99
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 1 cut(s) 148
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 2 cut(s) 47, 99
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 1 cut(s) 156
BspQI GCTCTTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 2 cut(s) 39, 65
BstBAI YACGTR 1 cut(s) 158
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 81
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 42
BstSLI GKGCMC 1 cut(s) 300
BstSNI TACGTA 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 149
BstV2I GAAGAC 2 cut(s) 23, 112
BsuRI GGCC 2 cut(s) 47, 99
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 215
CaiI CAGNNNCTG 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 97, 255
CviAII CATG 2 cut(s) 85, 184
CviJI RGCY 8 cut(s) 32, 40, 47, 99, 140, 154, 268, 288
CviKI_1 RGCY 8 cut(s) 32, 40, 47, 99, 140, 154, 268, 288
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 39, 65
EarI CTCTTC 2 cut(s) 39, 65
Eco105I TACGTA 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 255
Eco57I CTGAAG 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 2 cut(s) 88, 187
FaiI YATR 5 cut(s) 86, 180, 185, 245, 279
FatI CATG 2 cut(s) 84, 183
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 1 cut(s) 88
Fsp4HI GCNGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 138
HaeIII GGCC 2 cut(s) 47, 99
Hin1II CATG 2 cut(s) 88, 187
HphI GGTGA 1 cut(s) 320
Hpy188III TCNNGA 1 cut(s) 113
HpyAV CCTTC 2 cut(s) 58, 135
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 2 cut(s) 54, 171
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 2 cut(s) 88, 187
Kzo9I GATC 1 cut(s) 148
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 1 cut(s) 45
LpnPI CCDG 4 cut(s) 29, 56, 203, 271
Lsp1109I GCAGC 1 cut(s) 149
MaeII ACGT 1 cut(s) 157
MaeIII GTNAC 1 cut(s) 272
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 5 cut(s) 26, 28, 52, 112, 240
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 1 cut(s) 225
MnlI CCTC 6 cut(s) 31, 58, 66, 88, 109, 203
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 3 cut(s) 224, 320, 334
MspA1I CMGCKG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
NcoI CCATGG 1 cut(s) 183
NdeII GATC 1 cut(s) 148
NlaIII CATG 2 cut(s) 88, 187
NlaIV GGNNCC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 272
NspI RCATGY 1 cut(s) 88
PciI ACATGT 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 33
PkrI GCNGC 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 158
PpuMI RGGWCCY 1 cut(s) 255
PscI ACATGT 1 cut(s) 84
PshBI ATTAAT 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 2 cut(s) 97, 255
PspPPI RGGWCCY 1 cut(s) 255
PstNI CAGNNNCTG 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 3 cut(s) 224, 320, 334
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 2 cut(s) 97, 255
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 1 cut(s) 300
SetI ASST 8 cut(s) 23, 34, 42, 120, 142, 156, 160, 257
SinI GGWCC 1 cut(s) 255
SmlI CTYRAG 2 cut(s) 22, 113
SmoI CTYRAG 2 cut(s) 22, 113
SnaBI TACGTA 1 cut(s) 158
Sse9I AATT 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 63
TasI AATT 1 cut(s) 225
Tru1I TTAA 3 cut(s) 224, 320, 334
Tru9I TTAA 3 cut(s) 224, 320, 334
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 272
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 1 cut(s) 255
VspI ATTAAT 1 cut(s) 224
XceI RCATGY 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.