Rh5AG082600

Uncharacterised protein family UPF0052

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013634
Physical Location & Seq
Reverse (-)
7110519 .. 7112714
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG082600.1

Sequence Viewer

Length: 591 bp
ATGGCACTTGTCAATAAGGGAAGCTCTTCAGCTCCTGCACTCTCTTCGAGGATAAAGAGGGTATTCTACATGTCAAGTGAGGGCCAAAACTTGCTTCATGAGGTCTTCCCTGCTCCAAACCCAGGTGTGATGGATCAGCTACGTAATGTGGATTGCATTGTTTATGCCATGGGTTCTCTCTTTACTTCTGTTTGTCCATCACTGGTATTAATTGGCATTGGGGAGATTATATCTTCAAGGTCCTGCCCCAAGGTACTTTTGTTAAACGGTACCCATGATCGAGAGACAAGTGGCTTCTCAGCTTCTTGCTTTGTAACTGCTATTACAGATGCACTAAATCGAACATATGGAGCCCCTCATAACTGTCTCAAAAACCTTCCCAGTCATTATATCAATACCCTCTTGGTGCCCAAAGATGGTGAGATACCTGTTAATGTTCAATGCTTAGCTGACCAAGGAATTTTTGAAGTGATTGTTGTTGACTCTGTACATGATCCAAAAGTAGGTGTGGTATTTGACCCCAAGTCGTTAATACAAGCCCTTGATGATTTGATAGGCAGACACATGCCTACAAATGTTAAGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0052

Protein Analysis

196

Amino Acids

21.12

Weight (kDa)

6.28

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CofD PF01933 15 - 143 9.4e-16 2-phospho-L-lactate transferase CofD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 269
AccB1I GGYRCC 2 cut(s) 269, 406
AclWI GGATC 2 cut(s) 141, 488
AcsI RAATTY 1 cut(s) 459
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 3 cut(s) 255, 271, 489
AfiI CCNNNNNNNGG 3 cut(s) 122, 416, 503
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 3 cut(s) 237, 440, 467
AhdI GACNNNNNGTC 1 cut(s) 523
AjnI CCWGG 1 cut(s) 121
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 5 cut(s) 24, 32, 139, 302, 449
AluI AGCT 5 cut(s) 24, 32, 139, 302, 449
Alw26I GTCTC 2 cut(s) 278, 371
AlwI GGATC 2 cut(s) 141, 488
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 1 cut(s) 82
ApoI RAATTY 1 cut(s) 459
AseI ATTAAT 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 25
Asp718I GGTACC 1 cut(s) 269
AspS9I GGNCC 2 cut(s) 82, 240
AsuHPI GGTGA 1 cut(s) 431
AvaII GGWCC 1 cut(s) 240
BaeGI GKGCMC 1 cut(s) 411
BaeI ACNNNNGTAYC 2 cut(s) 245, 278
BanI GGYRCC 2 cut(s) 269, 406
BanII GRGCYC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 97
BccI CCATC 3 cut(s) 124, 205, 410
BcgI CGANNNNNNTGC 2 cut(s) 27, 61
BciT130I CCWGG 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 278, 371
BlpI GCTNAGC 1 cut(s) 445
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 240
BmeRI GACNNNNNGTC 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 82, 240
BmiI GGNNCC 3 cut(s) 271, 352, 408
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 375
BmsI GCATC 1 cut(s) 319
BmuI ACTGGG 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 97
Bpu1102I GCTNAGC 1 cut(s) 445
BsaAI YACGTR 1 cut(s) 143
BsaJI CCNNGG 4 cut(s) 121, 168, 249, 454
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 416, 503
Bse1I ACTGG 2 cut(s) 207, 381
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 121, 168, 249, 454
BseLI CCNNNNNNNGG 3 cut(s) 122, 416, 503
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 2 cut(s) 207, 381
BseSI GKGCMC 1 cut(s) 411
BsgI GTGCAG 1 cut(s) 21
BshFI GGCC 1 cut(s) 84
BshNI GGYRCC 2 cut(s) 269, 406
BslI CCNNNNNNNGG 3 cut(s) 122, 416, 503
BsmAI GTCTC 2 cut(s) 278, 371
BsnI GGCC 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 355, 411
Bsp1407I TGTACA 1 cut(s) 487
Bsp143I GATC 3 cut(s) 133, 277, 493
Bsp1720I GCTNAGC 1 cut(s) 445
Bsp19I CCATGG 1 cut(s) 168
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 311
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 3 cut(s) 271, 352, 408
BspPI GGATC 2 cut(s) 141, 488
BspQI GCTCTTC 1 cut(s) 31
BspT107I GGYRCC 2 cut(s) 269, 406
BsrGI TGTACA 1 cut(s) 487
BsrI ACTGG 2 cut(s) 207, 381
BssECI CCNNGG 4 cut(s) 121, 168, 249, 454
BssMI GATC 3 cut(s) 133, 277, 493
BssT1I CCWWGG 3 cut(s) 168, 249, 454
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 269, 365
Bst6I CTCTTC 2 cut(s) 31, 49
BstAUI TGTACA 1 cut(s) 487
BstBAI YACGTR 1 cut(s) 143
BstDEI CTNAG 2 cut(s) 298, 445
BstDSI CCRYGG 1 cut(s) 168
BstKTI GATC 3 cut(s) 136, 280, 496
BstMAI GTCTC 2 cut(s) 278, 371
BstMBI GATC 3 cut(s) 133, 277, 493
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 2 cut(s) 73, 568
BstSCI CCNGG 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 411
BstSNI TACGTA 1 cut(s) 143
BstV2I GAAGAC 1 cut(s) 97
BsuRI GGCC 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 200
CaiI CAGNNNCTG 1 cut(s) 35
CciI TCATGA 1 cut(s) 97
Cfr13I GGNCC 2 cut(s) 82, 240
Csp6I GTAC 3 cut(s) 254, 270, 488
CviAII CATG 6 cut(s) 70, 98, 169, 275, 491, 565
CviJI RGCY 9 cut(s) 24, 32, 84, 139, 294, 302, 353, 449, 539
CviKI_1 RGCY 9 cut(s) 24, 32, 84, 139, 294, 302, 353, 449, 539
CviQI GTAC 3 cut(s) 254, 270, 488
DdeI CTNAG 2 cut(s) 298, 445
DpnI GATC 3 cut(s) 135, 279, 495
DpnII GATC 3 cut(s) 133, 277, 493
DriI GACNNNNNGTC 1 cut(s) 523
Eam1104I CTCTTC 2 cut(s) 31, 49
Eam1105I GACNNNNNGTC 1 cut(s) 523
EarI CTCTTC 2 cut(s) 31, 49
Eco105I TACGTA 1 cut(s) 143
Eco130I CCWWGG 3 cut(s) 168, 249, 454
Eco24I GRGCYC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 3 cut(s) 168, 249, 454
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 3 cut(s) 168, 249, 454
FaeI CATG 6 cut(s) 73, 101, 172, 278, 494, 568
FatI CATG 6 cut(s) 69, 97, 168, 274, 490, 564
FauNDI CATATG 1 cut(s) 346
FriOI GRGCYC 1 cut(s) 355
HaeIII GGCC 1 cut(s) 84
Hin1II CATG 6 cut(s) 73, 101, 172, 278, 494, 568
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HinfI GANTC 1 cut(s) 482
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 481
Hpy188III TCNNGA 2 cut(s) 98, 281
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 1 cut(s) 386
HpyCH4III ACNGT 2 cut(s) 269, 365
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 3 cut(s) 38, 156, 332
HpyF3I CTNAG 2 cut(s) 298, 445
HpySE526I ACGT 1 cut(s) 142
Hsp92II CATG 6 cut(s) 73, 101, 172, 278, 494, 568
KpnI GGTACC 1 cut(s) 273
Kzo9I GATC 3 cut(s) 133, 277, 493
LguI GCTCTTC 1 cut(s) 31
LmnI GCTCC 3 cut(s) 37, 118, 350
LpnPI CCDG 8 cut(s) 48, 108, 123, 135, 188, 256, 394, 441
LweI GCATC 1 cut(s) 319
MaeII ACGT 1 cut(s) 142
MaeIII GTNAC 1 cut(s) 313
MalI GATC 3 cut(s) 135, 279, 495
MboI GATC 3 cut(s) 133, 277, 493
MboII GAAGA 4 cut(s) 18, 36, 97, 225
MhlI GDGCHC 2 cut(s) 355, 411
MluCI AATT 2 cut(s) 210, 459
MlyI GAGTC 1 cut(s) 476
MnlI CCTC 6 cut(s) 42, 51, 73, 94, 366, 410
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 5 cut(s) 209, 263, 432, 530, 579
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 168
NdeI CATATG 1 cut(s) 346
NdeII GATC 3 cut(s) 133, 277, 493
NlaIII CATG 6 cut(s) 73, 101, 172, 278, 494, 568
NlaIV GGNNCC 3 cut(s) 271, 352, 408
NspI RCATGY 2 cut(s) 73, 568
PagI TCATGA 1 cut(s) 97
PciI ACATGT 1 cut(s) 69
PciSI GCTCTTC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 25
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
Ppu21I YACGTR 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 240
PscI ACATGT 1 cut(s) 69
PshBI ATTAAT 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 3 cut(s) 271, 352, 408
PspPI GGNCC 2 cut(s) 82, 240
PspPPI RGGWCCY 1 cut(s) 240
PstNI CAGNNNCTG 1 cut(s) 35
RsaI GTAC 3 cut(s) 255, 271, 489
RsaNI GTAC 3 cut(s) 254, 270, 488
SapI GCTCTTC 1 cut(s) 31
SaqAI TTAA 5 cut(s) 209, 263, 432, 530, 579
Sau3AI GATC 3 cut(s) 133, 277, 493
Sau96I GGNCC 2 cut(s) 82, 240
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 2 cut(s) 355, 411
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 240
SnaBI TACGTA 1 cut(s) 143
Sse9I AATT 2 cut(s) 210, 459
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 3 cut(s) 168, 249, 454
TaaI ACNGT 2 cut(s) 269, 365
TaiI ACGT 1 cut(s) 145
TaqI TCGA 3 cut(s) 47, 280, 340
TasI AATT 2 cut(s) 210, 459
TatI WGTACW 1 cut(s) 487
Tru1I TTAA 5 cut(s) 209, 263, 432, 530, 579
Tru9I TTAA 5 cut(s) 209, 263, 432, 530, 579
TscAI CASTG 1 cut(s) 207
TspDTI ATGAA 1 cut(s) 86
TspRI CASTG 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 240
VspI ATTAAT 1 cut(s) 209
XapI RAATTY 1 cut(s) 459
XceI RCATGY 2 cut(s) 73, 568
XmnI GAANNNNTTC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.