RchiOBHm_Chr5g0012371

Glycine-rich RNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013632
Physical Location & Seq
Reverse (-)
8315748 .. 8319143
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29295

Sequence Viewer

Length: 330 bp
ATGTATGTAAGGAAAATGGGAAGGGCATCCTCGGGTTGGAGCAACTATGGGGGAGGAGATAGTTCATCTGCGGGGAGAAATGAATGCTTCAAGTGTGGCCGCCCAGGACATTGGGCACGAGATTGCCCTTCTGCAACTGATCAGGAAATGCATGGAAGTGGTCAAGCGGGAGGGGACGACTCAAACAATGATTACATTGTTTACTCATATGGTGGAAGGAGAAGCCCTTACTGGGAAAATGTCTGCTTCAAGTGTGGGTGGCCCGGACATTGGCAGCATGAGTGCCCTTCAGCAAATGGTGGAGGTGGAAACGACGATGACTCTGATTGA

Protein Analysis

109

Amino Acids

11.81

Weight (kDa)

5.43

Isoelectric Point (pI)

62.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 28 - 44 4.2e-09 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 71, 100, 167
AcoI YGGCCR 1 cut(s) 97
AcuI CTGAAG 1 cut(s) 273
AfiI CCNNNNNNNGG 3 cut(s) 36, 232, 270
AgsI TTSAA 2 cut(s) 91, 250
AjnI CCWGG 1 cut(s) 103
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 2 cut(s) 97, 260
ApeKI GCWGC 1 cut(s) 274
AspS9I GGNCC 1 cut(s) 261
AsuC2I CCSGG 1 cut(s) 264
AvaI CYCGRG 1 cut(s) 31
BaeGI GKGCMC 2 cut(s) 118, 287
BauI CACGAG 1 cut(s) 117
BbvI GCAGC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 105
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 1 cut(s) 264
BisI GCNGC 2 cut(s) 100, 275
BlsI GCNGC 2 cut(s) 101, 276
Bme1390I CCNGG 2 cut(s) 105, 264
BmeT110I CYCGRG 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 261
BmrFI CCNGG 2 cut(s) 105, 264
BmrI ACTGGG 1 cut(s) 241
BmsI GCATC 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 241
BpuMI CCSGG 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 30, 103
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 232, 270
Bse1I ACTGG 1 cut(s) 236
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 30, 103
BseGI GGATG 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 36, 232, 270
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 1 cut(s) 69
BseSI GKGCMC 2 cut(s) 118, 287
BseXI GCAGC 1 cut(s) 286
BshFI GGCC 2 cut(s) 99, 262
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 3 cut(s) 36, 232, 270
BsmFI GGGAC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 2 cut(s) 99, 262
BsoBI CYCGRG 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 118, 287
Bsp143I GATC 1 cut(s) 139
BspACI CCGC 3 cut(s) 71, 100, 167
BspANI GGCC 2 cut(s) 99, 262
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 30, 103
BssMI GATC 1 cut(s) 139
BssSI CACGAG 1 cut(s) 117
Bst2BI CACGAG 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 105
BstF5I GGATG 1 cut(s) 26
BstKTI GATC 1 cut(s) 142
BstMBI GATC 1 cut(s) 139
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 103, 262
BstSLI GKGCMC 2 cut(s) 118, 287
BstV1I GCAGC 1 cut(s) 286
BstXI CCANNNNNNTGG 1 cut(s) 111
BsuRI GGCC 2 cut(s) 99, 262
BtsCI GGATG 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 261
CviAII CATG 2 cut(s) 152, 278
CviJI RGCY 3 cut(s) 99, 225, 262
CviKI_1 RGCY 3 cut(s) 99, 225, 262
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 153
FaeI CATG 2 cut(s) 155, 281
FaiI YATR 6 cut(s) 6, 48, 153, 208, 210, 279
FaqI GGGAC 1 cut(s) 188
FatI CATG 2 cut(s) 151, 277
FauI CCCGC 2 cut(s) 64, 160
FauNDI CATATG 1 cut(s) 208
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 100, 275
FokI GGATG 1 cut(s) 13
Fsp4HI GCNGC 2 cut(s) 100, 275
GluI GCNGC 2 cut(s) 100, 275
HaeIII GGCC 2 cut(s) 99, 262
HapII CCGG 1 cut(s) 264
Hin1II CATG 2 cut(s) 155, 281
HinfI GANTC 2 cut(s) 179, 320
HpaII CCGG 1 cut(s) 264
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 4 cut(s) 15, 138, 210, 297
HpyCH4V TGCA 2 cut(s) 134, 151
Hsp92II CATG 2 cut(s) 155, 281
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 1 cut(s) 139
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 5 cut(s) 90, 117, 128, 217, 277
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 35
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MhlI GDGCHC 2 cut(s) 118, 287
MlyI GAGTC 2 cut(s) 173, 314
MmeI TCCRAC 1 cut(s) 17
MnlI CCTC 4 cut(s) 40, 47, 164, 296
Mph1103I ATGCAT 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 156
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 2 cut(s) 105, 264
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 1 cut(s) 105
NciI CCSGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 208
NdeII GATC 1 cut(s) 139
NlaIII CATG 2 cut(s) 155, 281
NsiI ATGCAT 1 cut(s) 153
PctI GAATGC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 101, 276
PleI GAGTC 2 cut(s) 173, 314
PpsI GAGTC 2 cut(s) 173, 314
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspPI GGNCC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 156
SatI GCNGC 2 cut(s) 100, 275
Sau3AI GATC 1 cut(s) 139
Sau96I GGNCC 1 cut(s) 261
SchI GAGTC 2 cut(s) 173, 314
ScrFI CCNGG 2 cut(s) 105, 264
SduI GDGCHC 2 cut(s) 118, 287
SetI ASST 1 cut(s) 307
SfaNI GCATC 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 156
SsiI CCGC 3 cut(s) 71, 100, 167
StyD4I CCNGG 2 cut(s) 103, 262
TauI GCSGC 1 cut(s) 102
TseI GCWGC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 54, 96
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.