Rw5G008310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013632
Physical Location & Seq
Reverse (-)
8916165 .. 8917310
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G008310.1

Sequence Viewer

Length: 471 bp
ATGAATGCTTCAAGTGTGGTCGTCCAGGACATTGGGCCAAAGATTGCCCTTCAGCAGGTCATGATCAAGACATGTATGGAAGGAAAATGGGAAGGGCACCCTTGGGTTGGTGCAACTATGGGGGAGGACATAGTTCATCTGTGGGGAGAAATGAATGCTTCAAGTGTGGCCGCCCAGGACATTGGGCACGAGATTGCCCTTCTGCAACTGATCAGGAAATGCATGGAAGTGGTCAAGCGGGAGGGGACGACTCAAACAATGACTACATTGTTTACTCATATGGTGGAAGGAGAAGCACTCACTGGGAAGATGACTGCTTCAAGTGTGGGCGCTCCGGACATTGGCAGCAAGAGTGCCCTTCGTCAAATGGTGGAGGTGGAAACGATATTGGCACTGATGGAACGCGCAGAGAAATTGAGGTTAGGTATACAAAGTGATATATACCCAAAAGAGTGCGACACCGGTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

16.99

Weight (kDa)

5.19

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 427
AccII CGCG 1 cut(s) 405
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 2 cut(s) 171, 238
AcoI YGGCCR 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 35
AfiI CCNNNNNNNGG 3 cut(s) 55, 107, 341
AflIII ACRYGT 1 cut(s) 71
AgeI ACCGGT 1 cut(s) 461
AgsI TTSAA 3 cut(s) 12, 162, 321
AjnI CCWGG 2 cut(s) 24, 174
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 2 cut(s) 35, 168
ApeKI GCWGC 1 cut(s) 345
AsiGI ACCGGT 1 cut(s) 461
AspLEI GCGC 2 cut(s) 332, 407
AspS9I GGNCC 1 cut(s) 35
BaeGI GKGCMC 3 cut(s) 99, 189, 358
BaeI ACNNNNGTAYC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 96
BauI CACGAG 1 cut(s) 188
BbvI GCAGC 1 cut(s) 357
BccI CCATC 1 cut(s) 391
BciT130I CCWGG 2 cut(s) 26, 176
BclI TGATCA 2 cut(s) 63, 210
BfoI RGCGCY 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 46
BisI GCNGC 2 cut(s) 171, 346
BlsI GCNGC 2 cut(s) 172, 347
Bme1390I CCNGG 2 cut(s) 26, 176
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 1 cut(s) 98
BmrFI CCNGG 2 cut(s) 26, 176
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BplI GAGNNNNNCTC 2 cut(s) 282, 314
BsaJI CCNNGG 2 cut(s) 101, 174
BsaWI WCCGGW 2 cut(s) 334, 461
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 107, 341
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 1 cut(s) 307
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 2 cut(s) 26, 176
BseDI CCNNGG 2 cut(s) 101, 174
BseLI CCNNNNNNNGG 3 cut(s) 55, 107, 341
BseNI ACTGG 1 cut(s) 307
BseSI GKGCMC 3 cut(s) 99, 189, 358
BseXI GCAGC 1 cut(s) 357
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 2 cut(s) 37, 170
BshNI GGYRCC 1 cut(s) 96
BshTI ACCGGT 1 cut(s) 461
BsiSI CCGG 2 cut(s) 335, 462
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 3 cut(s) 55, 107, 341
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 2 cut(s) 10, 160
BsnI GGCC 2 cut(s) 37, 170
Bsp1286I GDGCHC 3 cut(s) 99, 189, 358
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 2 cut(s) 63, 210
BspACI CCGC 2 cut(s) 171, 238
BspANI GGCC 2 cut(s) 37, 170
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 405
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 98
BspMI ACCTGC 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 1 cut(s) 307
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 2 cut(s) 101, 174
BssMI GATC 2 cut(s) 63, 210
BssNAI GTATAC 1 cut(s) 428
BssSI CACGAG 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 101
Bst1107I GTATAC 1 cut(s) 428
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 2 cut(s) 26, 176
BstENI CCTNNNNNAGG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 405
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 2 cut(s) 332, 407
BstKTI GATC 2 cut(s) 66, 213
BstMBI GATC 2 cut(s) 63, 210
BstNI CCWGG 2 cut(s) 26, 176
BstNSI RCATGY 1 cut(s) 75
BstSCI CCNGG 2 cut(s) 24, 174
BstSLI GKGCMC 3 cut(s) 99, 189, 358
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 357
BstXI CCANNNNNNTGG 2 cut(s) 32, 182
BstZ17I GTATAC 1 cut(s) 428
BsuRI GGCC 2 cut(s) 37, 170
BtsIMutI CAGTG 2 cut(s) 300, 392
BveI ACCTGC 1 cut(s) 46
CciI TCATGA 1 cut(s) 60
CfoI GCGC 2 cut(s) 332, 407
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 1 cut(s) 35
CspAI ACCGGT 1 cut(s) 461
CviAII CATG 3 cut(s) 61, 72, 223
CviJI RGCY 2 cut(s) 37, 170
CviKI_1 RGCY 2 cut(s) 37, 170
DpnI GATC 2 cut(s) 65, 212
DpnII GATC 2 cut(s) 63, 210
EaeI YGGCCR 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 53
EcoRII CCWGG 2 cut(s) 24, 174
EcoT14I CCWWGG 1 cut(s) 101
EcoT22I ATGCAT 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 3 cut(s) 64, 75, 226
FaqI GGGAC 1 cut(s) 259
FatI CATG 3 cut(s) 60, 71, 222
FauI CCCGC 1 cut(s) 231
FauNDI CATATG 1 cut(s) 279
FbaI TGATCA 2 cut(s) 63, 210
FblI GTMKAC 1 cut(s) 427
Fnu4HI GCNGC 2 cut(s) 171, 346
Fsp4HI GCNGC 2 cut(s) 171, 346
GlaI GCGC 2 cut(s) 331, 406
GluI GCNGC 2 cut(s) 171, 346
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 2 cut(s) 37, 170
HapII CCGG 2 cut(s) 335, 462
HhaI GCGC 2 cut(s) 332, 407
Hin1II CATG 3 cut(s) 64, 75, 226
Hin6I GCGC 2 cut(s) 330, 405
HinP1I GCGC 2 cut(s) 330, 405
HinfI GANTC 1 cut(s) 250
HpaII CCGG 2 cut(s) 335, 462
Hpy166II GTNNAC 2 cut(s) 273, 428
Hpy188III TCNNGA 4 cut(s) 61, 67, 214, 335
Hpy8I GTNNAC 2 cut(s) 273, 428
HpyAV CCTTC 6 cut(s) 59, 74, 86, 209, 281, 368
HpyCH4V TGCA 3 cut(s) 113, 205, 222
Hsp92II CATG 3 cut(s) 64, 75, 226
HspAI GCGC 2 cut(s) 330, 405
Kpn2I TCCGGA 1 cut(s) 334
Ksp22I TGATCA 2 cut(s) 63, 210
Kzo9I GATC 2 cut(s) 63, 210
LmnI GCTCC 1 cut(s) 337
LpnPI CCDG 8 cut(s) 11, 38, 41, 161, 188, 199, 288, 348
Lsp1109I GCAGC 1 cut(s) 357
MalI GATC 2 cut(s) 65, 212
MboI GATC 2 cut(s) 63, 210
MboII GAAGA 1 cut(s) 319
MhlI GDGCHC 3 cut(s) 99, 189, 358
MluCI AATT 1 cut(s) 413
MlyI GAGTC 1 cut(s) 244
MnlI CCTC 4 cut(s) 118, 235, 367, 411
Mph1103I ATGCAT 1 cut(s) 224
MroI TCCGGA 1 cut(s) 334
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 2 cut(s) 335, 462
MspR9I CCNGG 2 cut(s) 26, 176
Mva1269I GAATGC 2 cut(s) 10, 160
MvaI CCWGG 2 cut(s) 26, 176
MvnI CGCG 1 cut(s) 405
NdeI CATATG 1 cut(s) 279
NdeII GATC 2 cut(s) 63, 210
NlaIII CATG 3 cut(s) 64, 75, 226
NlaIV GGNNCC 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 224
NspI RCATGY 1 cut(s) 75
PagI TCATGA 1 cut(s) 60
PciI ACATGT 1 cut(s) 71
PctI GAATGC 2 cut(s) 10, 160
PfoI TCCNGGA 1 cut(s) 24
PinAI ACCGGT 1 cut(s) 461
PkrI GCNGC 2 cut(s) 172, 347
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PscI ACATGT 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 24, 174
PspGI CCWGG 2 cut(s) 24, 174
PspN4I GGNNCC 1 cut(s) 98
PspPI GGNCC 1 cut(s) 35
RseI CAYNNNNRTG 1 cut(s) 227
SatI GCNGC 2 cut(s) 171, 346
Sau3AI GATC 2 cut(s) 63, 210
Sau96I GGNCC 1 cut(s) 35
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 2 cut(s) 26, 176
SduI GDGCHC 3 cut(s) 99, 189, 358
SetI ASST 4 cut(s) 60, 378, 422, 427
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 1 cut(s) 413
SsiI CCGC 2 cut(s) 171, 238
StyD4I CCNGG 2 cut(s) 24, 174
StyI CCWWGG 1 cut(s) 101
TasI AATT 1 cut(s) 413
TauI GCSGC 1 cut(s) 173
TscAI CASTG 2 cut(s) 307, 399
TseI GCWGC 1 cut(s) 345
TspDTI ATGAA 3 cut(s) 17, 125, 167
TspRI CASTG 2 cut(s) 307, 399
XagI CCTNNNNNAGG 1 cut(s) 53
XceI RCATGY 1 cut(s) 75
XmiI GTMKAC 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.