RchiOBHm_Chr5g0013021

CorA-like Mg2+ transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002432
Physical Location & Seq
Forward (+)
8830363 .. 8835389
5027 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29357

Sequence Viewer

Length: 1557 bp
ATGCCTGGAAGTGAACTTTGGACAGACGGGCTTATCTGTGCTTTTGAGTTCATTCGAGGCCGAAAGAAACCAAGTGGTTTGAAATCTGGCTCAACAATCCTAAGCAGACAATATACAGATGTTGGTGACCATGAGAAGCTGCGGGTTCCTTCTAAAGGATTTCAAGAGTCTTCTTCCCCAATACATGATAGGAACAAGTCCCTAAGTGGTGATTACAAATACAGCCCAGCTCATGATGGTGAAAGAGTGGGTGATCATTGGGTGCCAATTGGGTGGGCTAGAATTTCAGAAATCGTCCCAACTGTGCAACCTGATGCCAGTTGGGATTCTCAACAGTTTGGGATTGATAATGAAGATGACTTCACTGTTGCAGACTTAGCAGCTCCATATTGGGAGCGTCCAGTGGGGCCTGTTTGGTGGTGCCATGTCTCTGCAGGTCACCCCTCAGTTGATGATTGGCTCCGTAATGCCCAGTGGCTACATCCTGCCGTCAGTTTAGCTTTAAGAGATGAAAGTCGACTGATTAGTGACAGGATGAAACACCTTTTATATGAGGTCCCAGTTAGAGTTTCTGGGGGGTTGTTATTTGAGCTCTTGGGACAGTCAGTTGGTAATCCATTTGTTGACGAGGATGATATACCCATTGTACTTCGTTCCTGGCAAGCACAAAACTTCCTTGTTACTGCTTTGCATGTGAAAGGGCCTGTATCAAGTATAAATGTGTTGGGCATCACAGAAGTTCAGGAACTTCTTTCGACTGGAGGCTATAATGTACCAAGAACAGTGCATGAAGTCATAGCACATCTAATTAGCCGCCTTACTCGATGGGATGATAGGCTATTCCGCAAGTCCATATTTGGTGCCGCAGATGAGATTGAATTGAAGTTTATGAACAGGAGAAACCATGAAGATATGAATCTCTTTAGCATAATTCTCAACCAAGAAATCAGAAAGTTATCGAGACAGGTCATCAGAGTAAAGTGGTCACTTCATGCAAGAGAGGAGATTTTGTTTGAGCTTCTTGAACATTTGAGAGGAAATACTACAAGAAGCTTGTTGGAGAAAATAAGAAAGAGCACAAGAGAAATGATTGAGGAGCAAGAAGCAGTTCGTGGCCGCTTATTTACAATTCAGGATGTGATGCAGAGCACTGTTCGTGCATGGTTGCAGGACAGAAGCCTAAGAGTGACACATAATCTTGCTGTTTTTGGCGGCTGTGGCCTTGTACTTTCCATCATCACTGGGCTTTTTGGAATCAATGTTGATGGAATGCCTGGAGCAAACAATGCACCATATGCATTCGGCGTATTTTCAGCCGTTCTCTTCTTTATTGGAATCGTGCTAATTCTACTTGGGTTGCTTTACCTTGGGTTGAAACAGCCTATTGCTGAAGAACAAGTTGAAGTTAGAAAGATGGAGCTTCAGGAGTTGGTTCAGATGTTTCAGCACGAGGCAGAGACTCATGCCCTGGTTCGTAAAAATGTGGCTCGGAATAATTTACCCCCAACACTGGGAGATGCATTTGCACGTAACGCAAATTATATCCTCATTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CorA

Protein Analysis

518

Amino Acids

58.64

Weight (kDa)

6.12

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CorA PF01544 314 - 450 9e-12 CorA-like Mg2+ transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 425
AccB1I GGYRCC 3 cut(s) 262, 420, 860
AccI GTMKAC 1 cut(s) 517
AciI CCGC 6 cut(s) 142, 814, 844, 864, 1117, 1212
AcoI YGGCCR 1 cut(s) 1114
AcsI RAATTY 1 cut(s) 282
AcuI CTGAAG 2 cut(s) 1406, 1410
AfaI GTAC 3 cut(s) 648, 774, 1227
AfiI CCNNNNNNNGG 3 cut(s) 155, 1510, 1511
AgsI TTSAA 7 cut(s) 82, 164, 878, 883, 1025, 1375, 1403
AjnI CCWGG 4 cut(s) 4, 656, 1273, 1467
AjuI GAANNNNNNNTTGG 1 cut(s) 33
AluBI AGCT 8 cut(s) 139, 230, 383, 500, 592, 1018, 1053, 1420
AluI AGCT 8 cut(s) 139, 230, 383, 500, 592, 1018, 1053, 1420
Alw21I GWGCWC 3 cut(s) 594, 1079, 1151
Alw26I GTCTC 3 cut(s) 433, 955, 1451
AoxI GGCC 5 cut(s) 58, 407, 701, 1114, 1219
ApeKI GCWGC 2 cut(s) 139, 380
ApoI RAATTY 1 cut(s) 282
Asp700I GAANNNNTTC 1 cut(s) 1107
AspS9I GGNCC 3 cut(s) 407, 556, 701
AsuHPI GGTGA 5 cut(s) 137, 221, 251, 263, 431
AvaII GGWCC 1 cut(s) 556
BanI GGYRCC 3 cut(s) 262, 420, 860
BanII GRGCYC 1 cut(s) 594
BauI CACGAG 1 cut(s) 1448
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 3 cut(s) 594, 1079, 1151
BbvI GCAGC 2 cut(s) 126, 392
BccI CCATC 5 cut(s) 230, 819, 1241, 1259, 1408
BceAI ACGGC 2 cut(s) 473, 1301
BciT130I CCWGG 4 cut(s) 6, 658, 1275, 1469
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 3 cut(s) 433, 955, 1451
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 425
BisI GCNGC 6 cut(s) 140, 381, 814, 864, 1117, 1213
BlsI GCNGC 6 cut(s) 141, 382, 815, 865, 1118, 1214
Bme1390I CCNGG 4 cut(s) 6, 658, 1275, 1469
Bme18I GGWCC 1 cut(s) 556
BmgT120I GGNCC 3 cut(s) 407, 556, 701
BmiI GGNNCC 7 cut(s) 147, 264, 408, 422, 461, 558, 862
BmrFI CCNGG 4 cut(s) 6, 658, 1275, 1469
BmrI ACTGGG 4 cut(s) 466, 554, 1251, 1520
BmsI GCATC 4 cut(s) 304, 738, 1131, 1507
BmuI ACTGGG 4 cut(s) 466, 554, 1251, 1520
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 2 cut(s) 780, 1296
Bpu10I CCTNAGC 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 1529
BsaBI GATNNNNATC 1 cut(s) 915
BsaJI CCNNGG 2 cut(s) 1366, 1467
BsaXI ACNNNNNCTCC 2 cut(s) 995, 1025
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 1510, 1511
Bse1I ACTGG 7 cut(s) 318, 401, 472, 560, 763, 1246, 1515
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 4 cut(s) 6, 658, 1275, 1469
BseDI CCNNGG 2 cut(s) 1366, 1467
BseGI GGATG 5 cut(s) 481, 540, 637, 835, 1141
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 3 cut(s) 155, 1510, 1511
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 7 cut(s) 318, 401, 472, 560, 763, 1246, 1515
BseRI GAGGAG 2 cut(s) 1016, 1109
BseXI GCAGC 2 cut(s) 126, 392
BseYI CCCAGC 1 cut(s) 226
BshFI GGCC 5 cut(s) 60, 409, 703, 1116, 1221
BshNI GGYRCC 3 cut(s) 262, 420, 860
BsiHKAI GWGCWC 3 cut(s) 594, 1079, 1151
BslFI GGGAC 4 cut(s) 184, 281, 542, 612
BslI CCNNNNNNNGG 3 cut(s) 155, 1510, 1511
BsmAI GTCTC 3 cut(s) 433, 955, 1451
BsmFI GGGAC 4 cut(s) 184, 281, 542, 612
BsmI GAATGC 2 cut(s) 1275, 1298
BsnI GGCC 5 cut(s) 60, 409, 703, 1116, 1221
Bsp1286I GDGCHC 3 cut(s) 594, 1079, 1151
Bsp143I GATC 1 cut(s) 253
BspACI CCGC 6 cut(s) 142, 814, 844, 864, 1117, 1212
BspANI GGCC 5 cut(s) 60, 409, 703, 1116, 1221
BspCNI CTCAG 1 cut(s) 458
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 7 cut(s) 147, 264, 408, 422, 461, 558, 862
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 425
BspT107I GGYRCC 3 cut(s) 262, 420, 860
BsrI ACTGG 7 cut(s) 318, 401, 472, 560, 763, 1246, 1515
BssECI CCNNGG 2 cut(s) 1366, 1467
BssMI GATC 1 cut(s) 253
BssSI CACGAG 1 cut(s) 1448
BssT1I CCWWGG 1 cut(s) 1366
Bst2BI CACGAG 1 cut(s) 1448
Bst2UI CCWGG 4 cut(s) 6, 658, 1275, 1469
Bst4CI ACNGT 6 cut(s) 304, 336, 367, 603, 784, 1153
Bst6I CTCTTC 1 cut(s) 1328
BstAPI GCANNNNNTGC 2 cut(s) 1286, 1295
BstBAI YACGTR 1 cut(s) 1529
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 5 cut(s) 101, 203, 376, 445, 1181
BstEII GGTNACC 2 cut(s) 125, 437
BstENI CCTNNNNNAGG 1 cut(s) 153
BstF5I GGATG 5 cut(s) 481, 540, 637, 835, 1141
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 3 cut(s) 433, 955, 1451
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 5 cut(s) 377, 1218, 1286, 1295, 1532
BstNI CCWGG 4 cut(s) 6, 658, 1275, 1469
BstNSI RCATGY 1 cut(s) 695
BstPI GGTNACC 2 cut(s) 125, 437
BstSCI CCNGG 4 cut(s) 4, 656, 1273, 1467
BstSFI CTRYAG 1 cut(s) 432
BstV1I GCAGC 2 cut(s) 126, 392
BstV2I GAAGAC 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 273
BsuRI GGCC 5 cut(s) 60, 409, 703, 1116, 1221
BtsCI GGATG 5 cut(s) 481, 540, 637, 835, 1141
BtsIMutI CAGTG 7 cut(s) 363, 408, 479, 789, 1149, 1239, 1508
BveI ACCTGC 1 cut(s) 425
Cac8I GCNNGC 1 cut(s) 663
CciI TCATGA 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 407, 556, 701
CseI GACGC 1 cut(s) 386
Csp6I GTAC 3 cut(s) 647, 773, 1226
CviQI GTAC 3 cut(s) 647, 773, 1226
DdeI CTNAG 5 cut(s) 101, 203, 376, 445, 1181
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 1114
Eam1104I CTCTTC 1 cut(s) 1328
EarI CTCTTC 1 cut(s) 1328
Ecl136II GAGCTC 1 cut(s) 592
Eco130I CCWWGG 1 cut(s) 1366
Eco24I GRGCYC 1 cut(s) 594
Eco47I GGWCC 1 cut(s) 556
Eco53kI GAGCTC 1 cut(s) 592
Eco57I CTGAAG 2 cut(s) 1406, 1410
Eco91I GGTNACC 2 cut(s) 125, 437
EcoICRI GAGCTC 1 cut(s) 592
EcoNI CCTNNNNNAGG 1 cut(s) 153
EcoO109I RGGNCCY 3 cut(s) 407, 556, 701
EcoO65I GGTNACC 2 cut(s) 125, 437
EcoRII CCWGG 4 cut(s) 4, 656, 1273, 1467
EcoT14I CCWWGG 1 cut(s) 1366
EcoT22I ATGCAT 2 cut(s) 1300, 1522
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 1366
FaqI GGGAC 4 cut(s) 184, 281, 542, 612
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 1294
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 6 cut(s) 140, 381, 814, 864, 1117, 1213
FokI GGATG 5 cut(s) 468, 547, 644, 842, 1148
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 6 cut(s) 140, 381, 814, 864, 1117, 1213
FspBI CTAG 1 cut(s) 279
GluI GCNGC 6 cut(s) 140, 381, 814, 864, 1117, 1213
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 2 cut(s) 780, 1296
HaeIII GGCC 5 cut(s) 60, 409, 703, 1116, 1221
HgaI GACGC 1 cut(s) 386
HincII GTYRAC 2 cut(s) 518, 625
HindII GTYRAC 2 cut(s) 518, 625
HindIII AAGCTT 1 cut(s) 1051
HinfI GANTC 6 cut(s) 167, 326, 916, 1254, 1335, 1459
HphI GGTGA 5 cut(s) 137, 221, 251, 263, 431
Hpy166II GTNNAC 3 cut(s) 14, 518, 625
Hpy188I TCNGA 5 cut(s) 289, 950, 974, 1437, 1491
Hpy188III TCNNGA 7 cut(s) 164, 233, 743, 960, 1022, 1133, 1424
Hpy8I GTNNAC 3 cut(s) 14, 518, 625
HpyAV CCTTC 1 cut(s) 159
HpyCH4III ACNGT 6 cut(s) 304, 336, 367, 603, 784, 1153
HpyCH4IV ACGT 1 cut(s) 1528
HpyF10VI GCNNNNNNNGC 5 cut(s) 377, 1218, 1286, 1295, 1532
HpyF3I CTNAG 5 cut(s) 101, 203, 376, 445, 1181
HpySE526I ACGT 1 cut(s) 1528
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 1 cut(s) 253
LmnI GCTCC 6 cut(s) 388, 394, 465, 1096, 1277, 1417
Lsp1109I GCAGC 2 cut(s) 126, 392
LweI GCATC 4 cut(s) 304, 738, 1131, 1507
MaeI CTAG 1 cut(s) 279
MaeII ACGT 1 cut(s) 1528
MaeIII GTNAC 7 cut(s) 125, 437, 527, 679, 984, 1186, 1529
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 6 cut(s) 162, 165, 365, 920, 1315, 1403
MfeI CAATTG 1 cut(s) 267
MhlI GDGCHC 3 cut(s) 594, 1079, 1151
MluCI AATT 9 cut(s) 267, 282, 807, 878, 930, 1128, 1344, 1495, 1537
MlyI GAGTC 2 cut(s) 176, 1453
MmeI TCCRAC 1 cut(s) 1038
Mph1103I ATGCAT 2 cut(s) 1300, 1522
MroXI GAANNNNTTC 1 cut(s) 1107
MseI TTAA 1 cut(s) 503
MslI CAYNNNNRTG 1 cut(s) 237
MspR9I CCNGG 4 cut(s) 6, 658, 1275, 1469
MunI CAATTG 1 cut(s) 267
Mva1269I GAATGC 2 cut(s) 1275, 1298
MvaI CCWGG 4 cut(s) 6, 658, 1275, 1469
MwoI GCNNNNNNNGC 5 cut(s) 377, 1218, 1286, 1295, 1532
NdeI CATATG 1 cut(s) 1294
NdeII GATC 1 cut(s) 253
NlaIV GGNNCC 7 cut(s) 147, 264, 408, 422, 461, 558, 862
NmuCI GTSAC 5 cut(s) 125, 437, 527, 984, 1186
NsiI ATGCAT 2 cut(s) 1300, 1522
NspI RCATGY 1 cut(s) 695
PagI TCATGA 1 cut(s) 232
PctI GAATGC 2 cut(s) 1275, 1298
PdmI GAANNNNTTC 1 cut(s) 1107
PfeI GAWTC 4 cut(s) 326, 916, 1254, 1335
PkrI GCNGC 6 cut(s) 141, 382, 815, 865, 1118, 1214
PleI GAGTC 2 cut(s) 175, 1453
PpsI GAGTC 2 cut(s) 175, 1453
Ppu21I YACGTR 1 cut(s) 1529
PpuMI RGGWCCY 1 cut(s) 556
Psp124BI GAGCTC 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 4 cut(s) 4, 656, 1273, 1467
PspEI GGTNACC 2 cut(s) 125, 437
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 4 cut(s) 4, 656, 1273, 1467
PspN4I GGNNCC 7 cut(s) 147, 264, 408, 422, 461, 558, 862
PspPI GGNCC 3 cut(s) 407, 556, 701
PspPPI RGGWCCY 1 cut(s) 556
PstI CTGCAG 1 cut(s) 436
RsaI GTAC 3 cut(s) 648, 774, 1227
RsaNI GTAC 3 cut(s) 647, 773, 1226
RseI CAYNNNNRTG 1 cut(s) 237
SacI GAGCTC 1 cut(s) 594
SalI GTCGAC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 6 cut(s) 140, 381, 814, 864, 1117, 1213
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 3 cut(s) 407, 556, 701
SchI GAGTC 2 cut(s) 176, 1453
ScrFI CCNGG 4 cut(s) 6, 658, 1275, 1469
SduI GDGCHC 3 cut(s) 594, 1079, 1151
SfaNI GCATC 4 cut(s) 304, 738, 1131, 1507
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 237
Sse9I AATT 9 cut(s) 267, 282, 807, 878, 930, 1128, 1344, 1495, 1537
SsiI CCGC 6 cut(s) 142, 814, 844, 864, 1117, 1212
SspMI CTAG 1 cut(s) 279
SstI GAGCTC 1 cut(s) 594
StyD4I CCNGG 4 cut(s) 4, 656, 1273, 1467
StyI CCWWGG 1 cut(s) 1366
TaaI ACNGT 6 cut(s) 304, 336, 367, 603, 784, 1153
TaiI ACGT 1 cut(s) 1531
TaqI TCGA 5 cut(s) 55, 517, 755, 823, 959
TasI AATT 9 cut(s) 267, 282, 807, 878, 930, 1128, 1344, 1495, 1537
TatI WGTACW 2 cut(s) 646, 1225
TauI GCSGC 4 cut(s) 816, 866, 1119, 1215
TfiI GAWTC 4 cut(s) 326, 916, 1254, 1335
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 7 cut(s) 370, 408, 479, 789, 1156, 1246, 1515
TseFI GTSAC 5 cut(s) 125, 437, 527, 984, 1186
TseI GCWGC 2 cut(s) 139, 380
Tsp45I GTSAC 5 cut(s) 125, 437, 527, 984, 1186
TspDTI ATGAA 9 cut(s) 40, 366, 525, 551, 804, 905, 921, 929, 980
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 7 cut(s) 370, 408, 479, 789, 1156, 1246, 1515
VpaK11BI GGWCC 1 cut(s) 556
XagI CCTNNNNNAGG 1 cut(s) 153
XapI RAATTY 1 cut(s) 282
XceI RCATGY 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 1107
XspI CTAG 1 cut(s) 279
Zsp2I ATGCAT 2 cut(s) 1300, 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.