RchiOBHm_Chr5g0053971

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013763
Physical Location & Seq
Forward (+)
56305299 .. 56305919
621 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33120

Sequence Viewer

Length: 327 bp
ATGATGAGAGAGTCCGTGTGGGGATACTTGCCATTCGATACTATAATAGAACGGCTGAGTAAGAGTGGGTCACCACCACCTTCCGTGTGTCTGGTTAAGTTCGAAATTGAATTTAACACCGGCAATGTGTGGTCTAACGAGATTACGGACGAAGGGCCCCCGATGCAAAGCTGCGGAGTCCACGTTGTAAATGATTGCAAAACTGCGGACTCCTGTCCACCGTTTTCCATAAGAGAGCTGAAGATCGATGCTGAAGAGACTGACTCCTACTACGATGCTGAAGAGATTGACTCCTACTACGACGCTGAAGATCGACTAACTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

108

Amino Acids

12.22

Weight (kDa)

4.12

Isoelectric Point (pI)

66.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 174, 206
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 4 cut(s) 260, 273, 300, 327
AgsI TTSAA 1 cut(s) 110
AluBI AGCT 2 cut(s) 171, 238
AluI AGCT 2 cut(s) 171, 238
Alw26I GTCTC 1 cut(s) 251
AoxI GGCC 1 cut(s) 155
ApaI GGGCCC 1 cut(s) 159
ApeKI GCWGC 1 cut(s) 171
ApoI RAATTY 1 cut(s) 110
ArsI GACNNNNNNTTYG 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 155, 156
AsuHPI GGTGA 1 cut(s) 63
AsuII TTCGAA 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 159
BanII GRGCYC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 158
BceAI ACGGC 1 cut(s) 68
BciVI GTATCC 1 cut(s) 17
BcoDI GTCTC 1 cut(s) 251
BfuI GTATCC 1 cut(s) 17
BisI GCNGC 1 cut(s) 172
BlsI GCNGC 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 155, 156
BmiI GGNNCC 2 cut(s) 157, 158
BmsI GCATC 3 cut(s) 153, 238, 265
BoxI GACNNNNGTC 1 cut(s) 213
BplI GAGNNNNNCTC 4 cut(s) 248, 280, 275, 307
Bpu14I TTCGAA 1 cut(s) 102
Bsa29I ATCGAT 1 cut(s) 246
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bse118I RCCGGY 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 130
BseCI ATCGAT 1 cut(s) 246
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 1 cut(s) 158
BshFI GGCC 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 246
BsiSI CCGG 1 cut(s) 120
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 1 cut(s) 157
Bsp119I TTCGAA 1 cut(s) 102
Bsp120I GGGCCC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 243, 310
BspACI CCGC 2 cut(s) 174, 206
BspANI GGCC 1 cut(s) 157
BspCNI CTCAG 1 cut(s) 48
BspDI ATCGAT 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 157, 158
BspT104I TTCGAA 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssMI GATC 2 cut(s) 243, 310
Bst4CI ACNGT 2 cut(s) 222, 322
Bst6I CTCTTC 2 cut(s) 249, 276
BstBI TTCGAA 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 56
BstEII GGTNACC 1 cut(s) 69
BstKTI GATC 2 cut(s) 246, 313
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 2 cut(s) 243, 310
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstPAI GACNNNNGTC 1 cut(s) 213
BstPI GGTNACC 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 158
Bsu15I ATCGAT 1 cut(s) 246
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 157
BsuTUI ATCGAT 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 155, 156
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 1 cut(s) 311
CviJI RGCY 4 cut(s) 55, 157, 171, 238
CviKI_1 RGCY 4 cut(s) 55, 157, 171, 238
DdeI CTNAG 1 cut(s) 56
DpnI GATC 2 cut(s) 245, 312
DpnII GATC 2 cut(s) 243, 310
Eam1104I CTCTTC 2 cut(s) 249, 276
EarI CTCTTC 2 cut(s) 249, 276
Eco24I GRGCYC 1 cut(s) 159
Eco57I CTGAAG 4 cut(s) 260, 273, 300, 327
Eco91I GGTNACC 1 cut(s) 69
EcoO109I RGGNCCY 2 cut(s) 155, 156
EcoO65I GGTNACC 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 159
FaiI YATR 2 cut(s) 44, 230
Fnu4HI GCNGC 1 cut(s) 172
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 172
GluI GCNGC 1 cut(s) 172
HaeIII GGCC 1 cut(s) 157
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 311
HinfI GANTC 5 cut(s) 11, 177, 209, 263, 290
HpaII CCGG 1 cut(s) 120
HphI GGTGA 1 cut(s) 63
Hpy166II GTNNAC 2 cut(s) 181, 218
Hpy8I GTNNAC 2 cut(s) 181, 218
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 90, 146
HpyCH4III ACNGT 2 cut(s) 222, 322
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 2 cut(s) 166, 198
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 183
Kzo9I GATC 2 cut(s) 243, 310
LpnPI CCDG 3 cut(s) 77, 133, 226
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 3 cut(s) 153, 238, 265
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 69
MalI GATC 2 cut(s) 245, 312
MboI GATC 2 cut(s) 243, 310
MboII GAAGA 4 cut(s) 253, 266, 293, 320
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 2 cut(s) 105, 110
MlyI GAGTC 5 cut(s) 20, 186, 203, 257, 284
MseI TTAA 2 cut(s) 96, 114
MspI CCGG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 163
NdeII GATC 2 cut(s) 243, 310
NlaIV GGNNCC 2 cut(s) 157, 158
NmuCI GTSAC 1 cut(s) 69
NspV TTCGAA 1 cut(s) 102
PkrI GCNGC 1 cut(s) 173
PleI GAGTC 5 cut(s) 19, 185, 203, 257, 284
PpsI GAGTC 5 cut(s) 19, 185, 203, 257, 284
PshAI GACNNNNGTC 1 cut(s) 213
PspEI GGTNACC 1 cut(s) 69
PspN4I GGNNCC 2 cut(s) 157, 158
PspOMI GGGCCC 1 cut(s) 155
PspPI GGNCC 2 cut(s) 155, 156
SaqAI TTAA 2 cut(s) 96, 114
SatI GCNGC 1 cut(s) 172
Sau3AI GATC 2 cut(s) 243, 310
Sau96I GGNCC 2 cut(s) 155, 156
SchI GAGTC 5 cut(s) 20, 186, 203, 257, 284
SduI GDGCHC 1 cut(s) 159
SetI ASST 4 cut(s) 82, 173, 186, 240
SfaNI GCATC 3 cut(s) 153, 238, 265
SfuI TTCGAA 1 cut(s) 102
SgeI CNNG 9 cut(s) 28, 40, 97, 104, 132, 151, 172, 194, 225
Sse9I AATT 2 cut(s) 105, 110
SsiI CCGC 2 cut(s) 174, 206
TaaI ACNGT 2 cut(s) 222, 322
TaiI ACGT 1 cut(s) 186
TaqI TCGA 4 cut(s) 36, 102, 246, 313
TasI AATT 2 cut(s) 105, 110
Tru1I TTAA 2 cut(s) 96, 114
Tru9I TTAA 2 cut(s) 96, 114
TseFI GTSAC 1 cut(s) 69
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 1 cut(s) 69
TspGWI ACGGA 3 cut(s) 4, 73, 161
XapI RAATTY 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.