RLG00000036881

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013763
Physical Location & Seq
Forward (+)
86096649 .. 86098682
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036881

Sequence Viewer

Length: 537 bp
ATGGTATACCCTGTTTTTTACAAGGTAGATCCATCAGATGTACAAAACCAACGCGGTAGTTTTGGTCAGGCACTTGCTCAACATGATAACTTGGAGAAGGTGGCGGGATGGAAGACAGCTCTTACAAAAGCAGGAAATCTTTCTGGGAGGCATTTCTCACACAGAGAAGAACGATCATTGAAATATGGAGGCCTACAAAAACTACGAAAGTTTATTCTCTCTAAGACTCTAGGTTTGAAAGAAGTAAAGGGTACAAACAAAGTTAAAGGAATCATGGTTCAGAACGACATACCAGATCAACAAATACCCTTGAATAAGGAAAGCTTCTTAAAGTTAAATGGTCTTCAAGTTCTTATAATTTCTGATGATATATTCTATGGAGATCATGTGGATTACCCTCCCAATGAGTTGAGTCTCTTTGACTGGAAGGATTGTCCATTACAATATTTTCCATCAGATTTTTATCCTAGCAAACTTGTTGTACTCAAGATGGATTTCAGTGAGATATCATCACCATGGAGTGCACTTAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

179

Amino Acids

20.28

Weight (kDa)

8.84

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 59 3.5e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 356
AccI GTMKAC 1 cut(s) 6
AccII CGCG 1 cut(s) 54
AciI CCGC 2 cut(s) 54, 104
AclWI GGATC 1 cut(s) 23
AfaI GTAC 3 cut(s) 42, 253, 483
AflII CTTAAG 1 cut(s) 527
AgsI TTSAA 4 cut(s) 181, 238, 313, 347
AloI GAACNNNNNNTCC 2 cut(s) 261, 293
AluBI AGCT 2 cut(s) 119, 324
AluI AGCT 2 cut(s) 119, 324
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 1 cut(s) 419
Alw44I GTGCAC 1 cut(s) 522
AlwI GGATC 1 cut(s) 23
AoxI GGCC 1 cut(s) 190
ApaLI GTGCAC 1 cut(s) 522
ArsI GACNNNNNNTTYG 2 cut(s) 217, 249
Asp700I GAANNNNTTC 1 cut(s) 139
AsuHPI GGTGA 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 526
BbsI GAAGAC 2 cut(s) 119, 335
Bbv12I GWGCWC 1 cut(s) 526
BccI CCATC 4 cut(s) 40, 102, 460, 484
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 230, 468
BfrI CTTAAG 1 cut(s) 527
BpiI GAAGAC 2 cut(s) 119, 335
BpuEI CTTGAG 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 515
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bse1I ACTGG 1 cut(s) 428
Bse8I GATNNNNATC 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 515
BseGI GGATG 1 cut(s) 113
BseJI GATNNNNATC 1 cut(s) 462
BseNI ACTGG 1 cut(s) 428
BseSI GKGCMC 1 cut(s) 526
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 526
BsmAI GTCTC 1 cut(s) 419
BsnI GGCC 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 526
Bsp1407I TGTACA 1 cut(s) 40
Bsp143I GATC 4 cut(s) 28, 173, 295, 382
Bsp19I CCATGG 1 cut(s) 515
BspACI CCGC 2 cut(s) 54, 104
BspANI GGCC 1 cut(s) 192
BspFNI CGCG 1 cut(s) 54
BspPI GGATC 1 cut(s) 23
BspTI CTTAAG 1 cut(s) 527
BsrGI TGTACA 1 cut(s) 40
BsrI ACTGG 1 cut(s) 428
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 4 cut(s) 28, 173, 295, 382
BssNAI GTATAC 1 cut(s) 7
BssT1I CCWWGG 1 cut(s) 515
Bst1107I GTATAC 1 cut(s) 7
BstAFI CTTAAG 1 cut(s) 527
BstAUI TGTACA 1 cut(s) 40
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 515
BstF5I GGATG 1 cut(s) 113
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 4 cut(s) 31, 176, 298, 385
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 4 cut(s) 28, 173, 295, 382
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 1 cut(s) 54
BstV2I GAAGAC 2 cut(s) 119, 335
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BstZ17I GTATAC 1 cut(s) 7
BsuRI GGCC 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 515
BtsCI GGATG 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 505
Csp6I GTAC 3 cut(s) 41, 252, 482
CviAII CATG 4 cut(s) 83, 274, 386, 516
CviJI RGCY 3 cut(s) 119, 192, 324
CviKI_1 RGCY 3 cut(s) 119, 192, 324
CviQI GTAC 3 cut(s) 41, 252, 482
DdeI CTNAG 1 cut(s) 222
DpnI GATC 4 cut(s) 30, 175, 297, 384
DpnII GATC 4 cut(s) 28, 173, 295, 382
Eco130I CCWWGG 1 cut(s) 515
Eco147I AGGCCT 1 cut(s) 192
Eco32I GATATC 1 cut(s) 507
EcoRV GATATC 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 515
FaeI CATG 4 cut(s) 86, 277, 389, 519
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FatI CATG 4 cut(s) 82, 273, 385, 515
FauI CCCGC 1 cut(s) 97
FblI GTMKAC 1 cut(s) 6
FokI GGATG 1 cut(s) 120
FspBI CTAG 2 cut(s) 230, 468
HaeIII GGCC 1 cut(s) 192
Hin1II CATG 4 cut(s) 86, 277, 389, 519
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 3 cut(s) 226, 270, 412
HphI GGTGA 1 cut(s) 504
Hpy166II GTNNAC 2 cut(s) 7, 524
Hpy188I TCNGA 4 cut(s) 37, 282, 364, 457
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 2 cut(s) 7, 524
HpyAV CCTTC 2 cut(s) 91, 421
HpyCH4V TGCA 1 cut(s) 524
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 4 cut(s) 86, 277, 389, 519
Kzo9I GATC 4 cut(s) 28, 173, 295, 382
LpnPI CCDG 6 cut(s) 24, 53, 117, 129, 306, 409
MaeI CTAG 2 cut(s) 230, 468
MalI GATC 4 cut(s) 30, 175, 297, 384
MboI GATC 4 cut(s) 28, 173, 295, 382
MboII GAAGA 3 cut(s) 124, 179, 335
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 1 cut(s) 357
MlyI GAGTC 2 cut(s) 220, 421
MnlI CCTC 3 cut(s) 141, 182, 408
MroXI GAANNNNTTC 1 cut(s) 139
MseI TTAA 4 cut(s) 264, 329, 335, 528
MslI CAYNNNNRTG 1 cut(s) 514
MspCI CTTAAG 1 cut(s) 527
MvnI CGCG 1 cut(s) 54
NcoI CCATGG 1 cut(s) 515
NdeII GATC 4 cut(s) 28, 173, 295, 382
NlaIII CATG 4 cut(s) 86, 277, 389, 519
PceI AGGCCT 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 270
PleI GAGTC 2 cut(s) 220, 420
PpsI GAGTC 2 cut(s) 220, 420
PsiI TTATAA 1 cut(s) 356
PsuI RGATCY 1 cut(s) 28
RsaI GTAC 3 cut(s) 42, 253, 483
RsaNI GTAC 3 cut(s) 41, 252, 482
RseI CAYNNNNRTG 1 cut(s) 514
SaqAI TTAA 4 cut(s) 264, 329, 335, 528
Sau3AI GATC 4 cut(s) 28, 173, 295, 382
SchI GAGTC 2 cut(s) 220, 421
SduI GDGCHC 1 cut(s) 526
SetI ASST 6 cut(s) 27, 102, 121, 235, 326, 534
SmiMI CAYNNNNRTG 1 cut(s) 514
SmlI CTYRAG 2 cut(s) 485, 527
SmoI CTYRAG 2 cut(s) 485, 527
Sse9I AATT 1 cut(s) 357
SseBI AGGCCT 1 cut(s) 192
SsiI CCGC 2 cut(s) 54, 104
SspI AATATT 1 cut(s) 446
SspMI CTAG 2 cut(s) 230, 468
StuI AGGCCT 1 cut(s) 192
StyI CCWWGG 1 cut(s) 515
TasI AATT 1 cut(s) 357
TatI WGTACW 2 cut(s) 40, 481
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 4 cut(s) 264, 329, 335, 528
Tru9I TTAA 4 cut(s) 264, 329, 335, 528
TscAI CASTG 1 cut(s) 505
TspRI CASTG 1 cut(s) 505
Vha464I CTTAAG 1 cut(s) 527
VneI GTGCAC 1 cut(s) 522
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 1 cut(s) 139
XspI CTAG 2 cut(s) 230, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.