RchiOBHm_Chr6g0275231

WPP domain-interacting tail-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002372
Physical Location & Seq
Reverse (-)
36927963 .. 36931533
3571 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24692

Sequence Viewer

Length: 1185 bp
ATGATATATCGCGTAAGAAAACGGAGCAGGGTGTATGGTAGCCCTAGCCCTAAAGGCCGCTATGGTGGAGGCCGTGGTAGGGTTCTTCCGAGGAGTCTTTTGGGTTGCAATCTTTGCCATGACTGCCGGTTTCAGAACCATTTGAATAGTTGCACAGTTAAAATGGATTATGATGCTACTCATGAAGCAAGTGATTTTGGCAACGATGTTAATATTGACGTCCCAGTAGTAGAATCAAATAGAGTTGATAGTCTTGAAAAGGTTTCATTTGATGGCATGGTTATAGAAGAATTACAAAATCTGGGCGAGGTTATAACTGGAGTTGAGTTGGATCTAGCATGTGCTTATGAGAAGTTGCTTAATTTAATTGGTTTTATGATGCATGTGGCAACCAAGGAGAGTGACTTTGATGCCTTTATATCCGATGAAGAGCAGACAATAGTTGGTTCTGTCGAGAAGGCTCTAGAGTTCACTCTCTTGTCTGGAATATTAGACTCTGAGGTAGGTGAATTGGATAAGGTCATAGCTTTACTTCAAATGGAAATTACGGCTGCGCAAGAGCTATTATCTTTATATACATACTTAGGAGAAACATTTACAGTAATAGAAGAAAAGCTGCAGGATTCTGAGCAGTCTTTGAAGCAGTTGCAAGAACAAATCTCTGAAATCAGGATGCAATCTCTGAAGTTCCAGAGGACCCTTTCATGCTTCGATGGAGAAGAAAATTGGAATAACAACAAAGGAGTACTGGGCCATTTAGAAGATGATATATTTTCAAGCAAGAATGTACTAATGAAAATGCAAACTGTTAAACATCAGAGACATATTTTGAGGATGCTAGATAAATCTTTGGCAAGAGAAATGGATCTTGAAAAGAAATTGACTGAATCAAAACAAATTGAAGAGGATCTAAAACTGAGACTACTCTCTTCAGAACAAGAGGCATATTGCATGGAGGATGAAATCAGTGAAATGAGAAATGTTGTTACAGATCTGAAAGAAAAAGAATCAAAAGTTGAAGATAGGGCTCGGAATGCAGAAGCCAAATATAAATTATTAGCAGAGACCAACACAGAACTTAATGAAGAGCTCAGACTTCTTAAGACACGGTTAACAGAATTGACATGCGGTTGCAACATGCCGTGGCAACTGTTGAGGCTAGGTAGGAGAAACAAAACATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0012505 GO:0016020 GO:0031090 GO:0031965 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

45.11

Weight (kDa)

5.03

Isoelectric Point (pI)

59.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIT1_2_N PF26581 99 - 237 3e-53 WIT1/2, N-terminal helical bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AatII GACGTC 1 cut(s) 222
Acc16I TGCGCA 1 cut(s) 555
AccII CGCG 1 cut(s) 12
AciI CCGC 2 cut(s) 58, 1128
AclWI GGATC 3 cut(s) 339, 873, 915
AcuI CTGAAG 2 cut(s) 704, 915
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 747, 789
AfiI CCNNNNNNNGG 1 cut(s) 79
AflII CTTAAG 1 cut(s) 1100
AflIII ACRYGT 1 cut(s) 1178
AgsI TTSAA 8 cut(s) 145, 257, 536, 640, 777, 872, 902, 1019
AluBI AGCT 4 cut(s) 527, 562, 616, 1090
AluI AGCT 4 cut(s) 527, 562, 616, 1090
Alw21I GWGCWC 1 cut(s) 1092
Alw26I GTCTC 3 cut(s) 814, 913, 1058
AlwI GGATC 3 cut(s) 339, 873, 915
AoxI GGCC 3 cut(s) 55, 70, 751
ApeKI GCWGC 2 cut(s) 551, 616
AspLEI GCGC 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 696, 751
AsuHPI GGTGA 1 cut(s) 518
AvaII GGWCC 1 cut(s) 696
BanII GRGCYC 2 cut(s) 1030, 1092
Bbv12I GWGCWC 1 cut(s) 1092
BbvI GCAGC 2 cut(s) 538, 603
BccI CCATC 2 cut(s) 266, 707
BceAI ACGGC 3 cut(s) 57, 564, 1126
BcoDI GTCTC 3 cut(s) 814, 913, 1058
BfaI CTAG 5 cut(s) 45, 335, 464, 839, 1160
BfmI CTRYAG 1 cut(s) 617
BfrI CTTAAG 1 cut(s) 1100
BglI GCCNNNNNGGC 1 cut(s) 54
BglII AGATCT 1 cut(s) 991
BisI GCNGC 3 cut(s) 58, 552, 617
BlsI GCNGC 3 cut(s) 59, 553, 618
BmcAI AGTACT 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 696
BmgT120I GGNCC 2 cut(s) 696, 751
BmiI GGNNCC 1 cut(s) 698
BmrI ACTGGG 2 cut(s) 218, 758
BmsI GCATC 5 cut(s) 163, 369, 400, 663, 825
BmuI ACTGGG 2 cut(s) 218, 758
BpmI CTGGAG 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 1058
BsaJI CCNNGG 4 cut(s) 73, 89, 393, 1142
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse118I RCCGGY 1 cut(s) 126
Bse1I ACTGG 3 cut(s) 224, 322, 753
BseDI CCNNGG 4 cut(s) 73, 89, 393, 1142
BseGI GGATG 3 cut(s) 678, 840, 964
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMII CTCAG 4 cut(s) 489, 618, 908, 1105
BseNI ACTGG 3 cut(s) 224, 322, 753
BseRI GAGGAG 1 cut(s) 106
BseXI GCAGC 2 cut(s) 538, 603
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 57, 72, 753
BsiHKAI GWGCWC 1 cut(s) 1092
BsiSI CCGG 1 cut(s) 127
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 3 cut(s) 814, 913, 1058
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 3 cut(s) 57, 72, 753
Bso31I GGTCTC 1 cut(s) 1058
Bsp1286I GDGCHC 2 cut(s) 1030, 1092
Bsp143I GATC 4 cut(s) 331, 865, 907, 991
BspACI CCGC 2 cut(s) 58, 1128
BspANI GGCC 3 cut(s) 57, 72, 753
BspCNI CTCAG 4 cut(s) 490, 619, 909, 1104
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 698
BspMAI CTGCAG 1 cut(s) 621
BspPI GGATC 3 cut(s) 339, 873, 915
BspQI GCTCTTC 2 cut(s) 423, 1080
BspTI CTTAAG 1 cut(s) 1100
BspTNI GGTCTC 1 cut(s) 1058
BsrFI RCCGGY 1 cut(s) 126
BsrI ACTGG 3 cut(s) 224, 322, 753
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 4 cut(s) 73, 89, 393, 1142
BssMI GATC 4 cut(s) 331, 865, 907, 991
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 393
Bst4CI ACNGT 5 cut(s) 157, 601, 808, 1110, 1152
Bst6I CTCTTC 4 cut(s) 423, 897, 934, 1080
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 1100
BstAPI GCANNNNNTGC 1 cut(s) 114
BstDEI CTNAG 5 cut(s) 498, 583, 627, 917, 1091
BstDSI CCRYGG 2 cut(s) 73, 1142
BstF5I GGATG 3 cut(s) 678, 840, 964
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 4 cut(s) 334, 868, 910, 994
BstMAI GTCTC 3 cut(s) 814, 913, 1058
BstMBI GATC 4 cut(s) 331, 865, 907, 991
BstMWI GCNNNNNNNGC 4 cut(s) 54, 114, 123, 1034
BstNSI RCATGY 5 cut(s) 342, 386, 1128, 1141, 1182
BstSFI CTRYAG 1 cut(s) 617
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 538, 603
BstX2I RGATCY 4 cut(s) 331, 865, 907, 991
BstYI RGATCY 4 cut(s) 331, 865, 907, 991
BsuRI GGCC 3 cut(s) 57, 72, 753
BtgI CCRYGG 2 cut(s) 73, 1142
BtsCI GGATG 3 cut(s) 678, 840, 964
BtsIMutI CAGTG 1 cut(s) 973
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 556
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 2 cut(s) 696, 751
Csp6I GTAC 2 cut(s) 746, 788
CviQI GTAC 2 cut(s) 746, 788
DdeI CTNAG 5 cut(s) 498, 583, 627, 917, 1091
DpnI GATC 4 cut(s) 333, 867, 909, 993
DpnII GATC 4 cut(s) 331, 865, 907, 991
Eam1104I CTCTTC 4 cut(s) 423, 897, 934, 1080
EarI CTCTTC 4 cut(s) 423, 897, 934, 1080
Ecl136II GAGCTC 1 cut(s) 1090
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 2 cut(s) 1030, 1092
Eco31I GGTCTC 1 cut(s) 1058
Eco47I GGWCC 1 cut(s) 696
Eco53kI GAGCTC 1 cut(s) 1090
Eco57I CTGAAG 2 cut(s) 704, 915
EcoICRI GAGCTC 1 cut(s) 1090
EcoO109I RGGNCCY 1 cut(s) 696
EcoT14I CCWWGG 1 cut(s) 393
EcoT22I ATGCAT 1 cut(s) 384
EcoT38I GRGCYC 2 cut(s) 1030, 1092
ErhI CCWWGG 1 cut(s) 393
FaqI GGGAC 1 cut(s) 206
Fnu4HI GCNGC 3 cut(s) 58, 552, 617
FokI GGATG 3 cut(s) 685, 847, 971
FriOI GRGCYC 2 cut(s) 1030, 1092
Fsp4HI GCNGC 3 cut(s) 58, 552, 617
FspBI CTAG 5 cut(s) 45, 335, 464, 839, 1160
FspI TGCGCA 1 cut(s) 555
GlaI GCGC 1 cut(s) 555
GluI GCNGC 3 cut(s) 58, 552, 617
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 3 cut(s) 57, 72, 753
HapII CCGG 1 cut(s) 127
HhaI GCGC 1 cut(s) 556
Hin1I GRCGYC 1 cut(s) 219
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HincII GTYRAC 1 cut(s) 1113
HindII GTYRAC 1 cut(s) 1113
HinfI GANTC 6 cut(s) 94, 233, 494, 623, 887, 1007
HpaI GTTAAC 1 cut(s) 1113
HpaII CCGG 1 cut(s) 127
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 2 cut(s) 471, 1113
Hpy188III TCNNGA 8 cut(s) 182, 254, 454, 464, 483, 670, 691, 869
Hpy8I GTNNAC 2 cut(s) 471, 1113
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 5 cut(s) 157, 601, 808, 1110, 1152
HpyCH4IV ACGT 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 114, 123, 1034
HpyF3I CTNAG 5 cut(s) 498, 583, 627, 917, 1091
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 219
HspAI GCGC 1 cut(s) 554
KspAI GTTAAC 1 cut(s) 1113
Kzo9I GATC 4 cut(s) 331, 865, 907, 991
LguI GCTCTTC 2 cut(s) 423, 1080
LmnI GCTCC 1 cut(s) 24
Lsp1109I GCAGC 2 cut(s) 538, 603
LweI GCATC 5 cut(s) 163, 369, 400, 663, 825
MaeI CTAG 5 cut(s) 45, 335, 464, 839, 1160
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 2 cut(s) 401, 985
MalI GATC 4 cut(s) 333, 867, 909, 993
MboI GATC 4 cut(s) 331, 865, 907, 991
MflI RGATCY 4 cut(s) 331, 865, 907, 991
MhlI GDGCHC 2 cut(s) 1030, 1092
MlyI GAGTC 2 cut(s) 103, 488
MmeI TCCRAC 1 cut(s) 309
Mph1103I ATGCAT 1 cut(s) 384
MseI TTAA 8 cut(s) 159, 210, 360, 365, 810, 1080, 1101, 1112
MspCI CTTAAG 1 cut(s) 1100
MspI CCGG 1 cut(s) 127
Mva1269I GAATGC 1 cut(s) 1039
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 4 cut(s) 54, 114, 123, 1034
NdeII GATC 4 cut(s) 331, 865, 907, 991
NlaIV GGNNCC 1 cut(s) 698
NmuCI GTSAC 1 cut(s) 401
NsbI TGCGCA 1 cut(s) 555
NsiI ATGCAT 1 cut(s) 384
NspI RCATGY 5 cut(s) 342, 386, 1128, 1141, 1182
PagI TCATGA 1 cut(s) 181
PciI ACATGT 1 cut(s) 1178
PciSI GCTCTTC 2 cut(s) 423, 1080
PctI GAATGC 1 cut(s) 1039
PfeI GAWTC 4 cut(s) 233, 623, 887, 1007
PkrI GCNGC 3 cut(s) 59, 553, 618
PleI GAGTC 2 cut(s) 102, 488
PpsI GAGTC 2 cut(s) 102, 488
PpuMI RGGWCCY 1 cut(s) 696
PscI ACATGT 1 cut(s) 1178
PsiI TTATAA 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 1092
Psp5II RGGWCCY 1 cut(s) 696
PspN4I GGNNCC 1 cut(s) 698
PspPI GGNCC 2 cut(s) 696, 751
PspPPI RGGWCCY 1 cut(s) 696
PstI CTGCAG 1 cut(s) 621
PsuI RGATCY 4 cut(s) 331, 865, 907, 991
RsaI GTAC 2 cut(s) 747, 789
RsaNI GTAC 2 cut(s) 746, 788
SacI GAGCTC 1 cut(s) 1092
SapI GCTCTTC 2 cut(s) 423, 1080
SaqAI TTAA 8 cut(s) 159, 210, 360, 365, 810, 1080, 1101, 1112
SatI GCNGC 3 cut(s) 58, 552, 617
Sau3AI GATC 4 cut(s) 331, 865, 907, 991
Sau96I GGNCC 2 cut(s) 696, 751
ScaI AGTACT 1 cut(s) 747
SchI GAGTC 2 cut(s) 103, 488
SduI GDGCHC 2 cut(s) 1030, 1092
SfaNI GCATC 5 cut(s) 163, 369, 400, 663, 825
SfcI CTRYAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SsiI CCGC 2 cut(s) 58, 1128
SspI AATATT 2 cut(s) 214, 489
SspMI CTAG 5 cut(s) 45, 335, 464, 839, 1160
SstI GAGCTC 1 cut(s) 1092
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 5 cut(s) 157, 601, 808, 1110, 1152
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 453, 711
TatI WGTACW 2 cut(s) 745, 787
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 4 cut(s) 233, 623, 887, 1007
Tru1I TTAA 8 cut(s) 159, 210, 360, 365, 810, 1080, 1101, 1112
Tru9I TTAA 8 cut(s) 159, 210, 360, 365, 810, 1080, 1101, 1112
TscAI CASTG 1 cut(s) 973
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 2 cut(s) 551, 616
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 7 cut(s) 198, 255, 441, 693, 809, 975, 1098
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 1 cut(s) 973
Vha464I CTTAAG 1 cut(s) 1100
VpaK11BI GGWCC 1 cut(s) 696
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 5 cut(s) 342, 386, 1128, 1141, 1182
XspI CTAG 5 cut(s) 45, 335, 464, 839, 1160
ZraI GACGTC 1 cut(s) 220
ZrmI AGTACT 1 cut(s) 747
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.