Rroxscaffold_7G00193530

WPP domain-interacting tail-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002372
Physical Location & Seq
Forward (+)
35052106 .. 35055638
3533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193530.1

Sequence Viewer

Length: 1119 bp
ATGGTAGCCCTAGCCCTAAAGGCCGCTATGGTGGAGGCCGTGATTAGTGGGATTTGGATTCGGTTTCAGAACCATTTGAATAGTTGCACAGTTAAAATGGATTATGATGCTCCTCATAAAGCAAGTGATTTTGGCAACGATGTTAATATTGACGTCCCAGTAGTAGAATCAAATAGAGTTGATAGTCTTGAAAGGGTTTCATTTGATGGCACGGTTATAGGAGAATTACAAAATGTGGGCGAGGTTATAACTGGAGTTGAGTTGGATCTAGCATGTGCTTCTGAGAAGTTGCTTAATTTAATTGGTTTTATGATGCATGTGGCAACCAAGGAGAGTGACTTTGATGCCTTTACATCCAAGGAAGAGCAGACAATAGTTGGTTCTGTCGAGAAGGCTCTAGAGTACACTCTCTTATCTGGAATATTAGACTCTGAGGTAGGTGAATTGGATAAGGTCATAGATTTACTTCAAATGGAGATTACGGCTGCTCTAGAGCTACTATCTTTATATACATACTTAGGAGAAACATTTATAGTAATGGAAGAAAAGCTGCAGGATTCTGAGCAATCTTTGAAGCAGTTGCAAGAACAAATCTCTGAAATCAGGATGCAATCTCTGAAGTTCCAGAGGACTCTTTCAGGCTCCGATGGAGAAGAAAATTGGAATGACAACAAAGGAGTACTGGGCCATTTAGAAGATGATATATTTTCAAGCAAGAATGTACTAATGAAAATGCAAACTGTTAAACATCAGCGACATATTTTGAGGATGCTAGAGAAATCTTTGGCAAGAGAAATGGATCTTGAAAAGAAATTGACTGAATCAAAACAAATTGAAGAGGATCTAAAACTGAGACTACTCTGTTCAGAACAAGAGGCATATTGCATGGAGGATGAAATCAGTGAACTGAGAAATGTTATTACAGATCTGAAAGAAAAAGAATCAAAAGTTGAAGATAGGGCTCGGAATGCAGAGGCCAAATATAAATTATTAGCAAAGACCAACACAGAACTTAATGAAGAGCTCAGACTTCTTAAGACACAGTTAACAGAATTGACATGCGGTTGCAACATGCCGTGGCAACTGCTGAAGCTACGTAGGAGAAACAAAACATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0012505 GO:0016020 GO:0031090 GO:0031965 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

372

Amino Acids

42.35

Weight (kDa)

4.83

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIT1_2_N PF26581 77 - 213 4.6e-53 WIT1/2, N-terminal helical bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 248
AatII GACGTC 1 cut(s) 156
AciI CCGC 2 cut(s) 24, 1062
AclWI GGATC 3 cut(s) 273, 807, 849
AcuI CTGAAG 2 cut(s) 638, 1109
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 404, 681, 723
AflII CTTAAG 1 cut(s) 1034
AflIII ACRYGT 1 cut(s) 1112
AgsI TTSAA 8 cut(s) 79, 191, 470, 574, 711, 806, 836, 953
AluBI AGCT 4 cut(s) 496, 550, 1024, 1093
AluI AGCT 4 cut(s) 496, 550, 1024, 1093
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 1 cut(s) 847
AlwI GGATC 3 cut(s) 273, 807, 849
AoxI GGCC 4 cut(s) 21, 36, 685, 975
ApeKI GCWGC 2 cut(s) 485, 550
AspS9I GGNCC 1 cut(s) 685
AsuHPI GGTGA 1 cut(s) 452
BanII GRGCYC 2 cut(s) 964, 1026
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 2 cut(s) 472, 537
BccI CCATC 2 cut(s) 200, 641
BceAI ACGGC 3 cut(s) 23, 498, 1060
BcoDI GTCTC 1 cut(s) 847
BfaI CTAG 5 cut(s) 11, 269, 398, 491, 773
BfmI CTRYAG 1 cut(s) 551
BfrI CTTAAG 1 cut(s) 1034
BglI GCCNNNNNGGC 1 cut(s) 20
BglII AGATCT 1 cut(s) 925
BisI GCNGC 3 cut(s) 24, 486, 551
BlsI GCNGC 3 cut(s) 25, 487, 552
BmcAI AGTACT 1 cut(s) 681
BmgT120I GGNCC 1 cut(s) 685
BmiI GGNNCC 1 cut(s) 643
BmrI ACTGGG 2 cut(s) 152, 692
BmsI GCATC 5 cut(s) 97, 303, 334, 597, 759
BmuI ACTGGG 2 cut(s) 152, 692
BpmI CTGGAG 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 1097
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 3 cut(s) 327, 357, 1076
Bse1I ACTGG 3 cut(s) 158, 256, 687
BseDI CCNNGG 3 cut(s) 327, 357, 1076
BseGI GGATG 4 cut(s) 353, 612, 774, 898
BseMII CTCAG 6 cut(s) 273, 423, 552, 842, 899, 1039
BseNI ACTGG 3 cut(s) 158, 256, 687
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 472, 537
BshFI GGCC 4 cut(s) 23, 38, 687, 977
BsiHKAI GWGCWC 1 cut(s) 1026
BslFI GGGAC 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 847
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 4 cut(s) 23, 38, 687, 977
Bsp1286I GDGCHC 2 cut(s) 964, 1026
Bsp143I GATC 4 cut(s) 265, 799, 841, 925
BspACI CCGC 2 cut(s) 24, 1062
BspANI GGCC 4 cut(s) 23, 38, 687, 977
BspCNI CTCAG 6 cut(s) 274, 424, 553, 843, 900, 1038
BspLI GGNNCC 1 cut(s) 643
BspMAI CTGCAG 1 cut(s) 555
BspPI GGATC 3 cut(s) 273, 807, 849
BspQI GCTCTTC 2 cut(s) 357, 1014
BspTI CTTAAG 1 cut(s) 1034
BsrI ACTGG 3 cut(s) 158, 256, 687
BssECI CCNNGG 3 cut(s) 327, 357, 1076
BssMI GATC 4 cut(s) 265, 799, 841, 925
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 2 cut(s) 327, 357
Bst4CI ACNGT 4 cut(s) 91, 214, 742, 1044
Bst6I CTCTTC 3 cut(s) 357, 831, 1014
BstACI GRCGYC 1 cut(s) 153
BstAFI CTTAAG 1 cut(s) 1034
BstBAI YACGTR 1 cut(s) 1097
BstDEI CTNAG 7 cut(s) 282, 432, 517, 561, 851, 908, 1025
BstDSI CCRYGG 1 cut(s) 1076
BstF5I GGATG 4 cut(s) 353, 612, 774, 898
BstKTI GATC 4 cut(s) 268, 802, 844, 928
BstMAI GTCTC 1 cut(s) 847
BstMBI GATC 4 cut(s) 265, 799, 841, 925
BstMWI GCNNNNNNNGC 2 cut(s) 20, 968
BstNSI RCATGY 5 cut(s) 276, 320, 1062, 1075, 1116
BstSFI CTRYAG 1 cut(s) 551
BstSNI TACGTA 1 cut(s) 1097
BstV1I GCAGC 2 cut(s) 472, 537
BstX2I RGATCY 4 cut(s) 265, 799, 841, 925
BstYI RGATCY 4 cut(s) 265, 799, 841, 925
BsuRI GGCC 4 cut(s) 23, 38, 687, 977
BtgI CCRYGG 1 cut(s) 1076
BtsCI GGATG 4 cut(s) 353, 612, 774, 898
BtsIMutI CAGTG 1 cut(s) 907
Cfr13I GGNCC 1 cut(s) 685
Csp6I GTAC 3 cut(s) 403, 680, 722
CviAII CATG 6 cut(s) 273, 317, 886, 1059, 1072, 1113
CviQI GTAC 3 cut(s) 403, 680, 722
DdeI CTNAG 7 cut(s) 282, 432, 517, 561, 851, 908, 1025
DpnI GATC 4 cut(s) 267, 801, 843, 927
DpnII GATC 4 cut(s) 265, 799, 841, 925
Eam1104I CTCTTC 3 cut(s) 357, 831, 1014
EarI CTCTTC 3 cut(s) 357, 831, 1014
Ecl136II GAGCTC 1 cut(s) 1024
Eco105I TACGTA 1 cut(s) 1097
Eco130I CCWWGG 2 cut(s) 327, 357
Eco24I GRGCYC 2 cut(s) 964, 1026
Eco53kI GAGCTC 1 cut(s) 1024
Eco57I CTGAAG 2 cut(s) 638, 1109
EcoICRI GAGCTC 1 cut(s) 1024
EcoT14I CCWWGG 2 cut(s) 327, 357
EcoT22I ATGCAT 1 cut(s) 318
EcoT38I GRGCYC 2 cut(s) 964, 1026
ErhI CCWWGG 2 cut(s) 327, 357
FaeI CATG 6 cut(s) 276, 320, 889, 1062, 1075, 1116
FaqI GGGAC 1 cut(s) 140
FatI CATG 6 cut(s) 272, 316, 885, 1058, 1071, 1112
Fnu4HI GCNGC 3 cut(s) 24, 486, 551
FokI GGATG 4 cut(s) 340, 619, 781, 905
FriOI GRGCYC 2 cut(s) 964, 1026
Fsp4HI GCNGC 3 cut(s) 24, 486, 551
FspBI CTAG 5 cut(s) 11, 269, 398, 491, 773
GluI GCNGC 3 cut(s) 24, 486, 551
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 4 cut(s) 23, 38, 687, 977
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 6 cut(s) 276, 320, 889, 1062, 1075, 1116
HincII GTYRAC 1 cut(s) 1047
HindII GTYRAC 1 cut(s) 1047
HinfI GANTC 7 cut(s) 58, 167, 428, 557, 631, 821, 941
HpaI GTTAAC 1 cut(s) 1047
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 3 cut(s) 405, 905, 1047
Hpy188III TCNNGA 8 cut(s) 188, 388, 398, 417, 491, 604, 625, 803
Hpy8I GTNNAC 3 cut(s) 405, 905, 1047
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 4 cut(s) 91, 214, 742, 1044
HpyCH4IV ACGT 2 cut(s) 153, 1096
HpyCH4V TGCA 9 cut(s) 87, 316, 553, 583, 610, 736, 885, 971, 1068
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 968
HpyF3I CTNAG 7 cut(s) 282, 432, 517, 561, 851, 908, 1025
HpySE526I ACGT 2 cut(s) 153, 1096
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 6 cut(s) 276, 320, 889, 1062, 1075, 1116
KspAI GTTAAC 1 cut(s) 1047
Kzo9I GATC 4 cut(s) 265, 799, 841, 925
LguI GCTCTTC 2 cut(s) 357, 1014
LmnI GCTCC 2 cut(s) 115, 647
LpnPI CCDG 8 cut(s) 171, 237, 402, 539, 589, 624, 638, 668
Lsp1109I GCAGC 2 cut(s) 472, 537
LweI GCATC 5 cut(s) 97, 303, 334, 597, 759
MaeI CTAG 5 cut(s) 11, 269, 398, 491, 773
MaeII ACGT 2 cut(s) 153, 1096
MaeIII GTNAC 1 cut(s) 335
MalI GATC 4 cut(s) 267, 801, 843, 927
MboI GATC 4 cut(s) 265, 799, 841, 925
MboII GAAGA 7 cut(s) 374, 554, 665, 707, 848, 965, 1031
MflI RGATCY 4 cut(s) 265, 799, 841, 925
MhlI GDGCHC 2 cut(s) 964, 1026
MluCI AATT 9 cut(s) 224, 295, 300, 443, 658, 812, 831, 986, 1052
MlyI GAGTC 2 cut(s) 422, 625
MmeI TCCRAC 1 cut(s) 243
Mph1103I ATGCAT 1 cut(s) 318
MseI TTAA 8 cut(s) 93, 144, 294, 299, 744, 1014, 1035, 1046
MspCI CTTAAG 1 cut(s) 1034
Mva1269I GAATGC 1 cut(s) 973
MwoI GCNNNNNNNGC 2 cut(s) 20, 968
NdeII GATC 4 cut(s) 265, 799, 841, 925
NlaIII CATG 6 cut(s) 276, 320, 889, 1062, 1075, 1116
NlaIV GGNNCC 1 cut(s) 643
NmuCI GTSAC 1 cut(s) 335
NsiI ATGCAT 1 cut(s) 318
NspI RCATGY 5 cut(s) 276, 320, 1062, 1075, 1116
PciI ACATGT 1 cut(s) 1112
PciSI GCTCTTC 2 cut(s) 357, 1014
PctI GAATGC 1 cut(s) 973
PfeI GAWTC 5 cut(s) 58, 167, 557, 821, 941
PkrI GCNGC 3 cut(s) 25, 487, 552
PleI GAGTC 2 cut(s) 422, 625
PpsI GAGTC 2 cut(s) 422, 625
Ppu21I YACGTR 1 cut(s) 1097
PscI ACATGT 1 cut(s) 1112
PsiI TTATAA 1 cut(s) 248
Psp124BI GAGCTC 1 cut(s) 1026
PspN4I GGNNCC 1 cut(s) 643
PspPI GGNCC 1 cut(s) 685
PstI CTGCAG 1 cut(s) 555
PsuI RGATCY 4 cut(s) 265, 799, 841, 925
RsaI GTAC 3 cut(s) 404, 681, 723
RsaNI GTAC 3 cut(s) 403, 680, 722
SacI GAGCTC 1 cut(s) 1026
SapI GCTCTTC 2 cut(s) 357, 1014
SaqAI TTAA 8 cut(s) 93, 144, 294, 299, 744, 1014, 1035, 1046
SatI GCNGC 3 cut(s) 24, 486, 551
Sau3AI GATC 4 cut(s) 265, 799, 841, 925
Sau96I GGNCC 1 cut(s) 685
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 2 cut(s) 422, 625
SduI GDGCHC 2 cut(s) 964, 1026
SfaNI GCATC 5 cut(s) 97, 303, 334, 597, 759
SfcI CTRYAG 1 cut(s) 551
SmlI CTYRAG 1 cut(s) 1034
SmoI CTYRAG 1 cut(s) 1034
SnaBI TACGTA 1 cut(s) 1097
Sse9I AATT 9 cut(s) 224, 295, 300, 443, 658, 812, 831, 986, 1052
SsiI CCGC 2 cut(s) 24, 1062
SspI AATATT 2 cut(s) 148, 423
SspMI CTAG 5 cut(s) 11, 269, 398, 491, 773
SstI GAGCTC 1 cut(s) 1026
StyI CCWWGG 2 cut(s) 327, 357
TaaI ACNGT 4 cut(s) 91, 214, 742, 1044
TaiI ACGT 2 cut(s) 156, 1099
TaqI TCGA 1 cut(s) 387
TasI AATT 9 cut(s) 224, 295, 300, 443, 658, 812, 831, 986, 1052
TatI WGTACW 3 cut(s) 402, 679, 721
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 5 cut(s) 58, 167, 557, 821, 941
Tru1I TTAA 8 cut(s) 93, 144, 294, 299, 744, 1014, 1035, 1046
Tru9I TTAA 8 cut(s) 93, 144, 294, 299, 744, 1014, 1035, 1046
TscAI CASTG 1 cut(s) 907
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 2 cut(s) 485, 550
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 4 cut(s) 189, 743, 909, 1032
TspRI CASTG 1 cut(s) 907
Vha464I CTTAAG 1 cut(s) 1034
XbaI TCTAGA 2 cut(s) 397, 490
XceI RCATGY 5 cut(s) 276, 320, 1062, 1075, 1116
XspI CTAG 5 cut(s) 11, 269, 398, 491, 773
ZraI GACGTC 1 cut(s) 154
ZrmI AGTACT 1 cut(s) 681
Zsp2I ATGCAT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.