Rorug02G0508300

sodium-coupled neutral amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000216
Physical Location & Seq
Forward (+)
63955221 .. 63965278
10058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0508300.1

Sequence Viewer

Length: 3732 bp
ATGTATCTGAATTGCAGGGTGTCTGGTTTTAATGGCAGACTACCTGCTAATTTCAAGTTAAACAAGGCAATGGAGCCCTTGGATGGGCCTAATTGTGTCAGTTATTCGAGAAGAAAGCTTCTACTTCTTGGGATTTTAGGTTTTATTACAGTAGTGTGGTTTTTCCTGGGTTTCAATGAGGGACTTCTGGGGATGAAAGAGAAGACTCCAGAGATGTGTGGAGAGAAAGCTCGAATCTTGCAGCAGCATTTCAATGTCAGCAAGAACCAGCTTCTGGCTTTAGCTTCCTTGTTCTCCGAATCAGATCAGATAGCATCCCTTGAATGTAACAAAGAAACAGGACATGGGATGCTTTTAACTGATGGCATTTCCTGTGCTTTGAAGGCAGTATGTTCAGATGAGAAAGAATTTCAAAAGCATCATAAATGGGTGGCAGAATATGTTGAAGCCAAGGACCAATGCCTGGTTCAAGATGAAAACATCCCCAGGAGGCTTGACCTATCAGTGCTACAGGAAAAGTCCTTGGCACACATTCCGCAGTCTACAGTATCTGCTAATAGAATTTGTCAAAAGGACAATTTGGGATCAGGGGCAAAGGTAGAGTGCACAAAAGTGGATTCTCAGATTTTATGTTGTTTGATAAAGTGTTGTTCGTGGGTTTTTGTTGGAATGATTTTGTTCTACCAATTGTCTGGTTTTTCTTTGAAATTATGGAGGAACCAGAAGCAGAAGCTGGTTCATGAGTGCCCATGCCAACAGAAATTGGTTCAAGAGCAGCCATTGGGTCGTAAGAAGCAGCCAGAGAAACAGCAACAAGTCGTCCACAGTCCTCCTAAGGTTGCTTGCACGTGGAGGAAGAAACTCCTAATCGTATTTGTCTTATTTGGAGTTATTACGTCAATTTGGTTATTTTTCCACTTGAATGAAAGAGATTTTGTGTGGAGAGAAGAAACACTTTCCAACATGTGTGATGAACGAGCCCGGATGTTGCAAGATCAGTTCAATGTGAGCCTGAACCATGTTCATGCTTTAGCTATTCTCGTCTCCACCTTTCACCATGGAAAGCATCCTTCTGCCATTGATCAGAAAACATTTGGTGAATATACAGAGAGAACAGCTTTTGAGAGACCACTTACTAGTGGTGTTGCTTATGCTTTAAAAGTTACTCATGCTGAGAGGGAACAATTTGAGAAGGAACATGGATGGACAATAAAGAAAATGGAAACTGAGGACCAGACTCTTGTCCAAGATTTTCTTCCAGAAAGCTTGGATCCTGCCCCTATTCAAGATGAATATGCGCCTGTGATATTTTCTCAAGAAACTGTCTCCCATATTGTCTCGATTGACATGATGTCGGGAAAGGATGACCGCGAGAACATCTTGCGAGCAAGGTCAACAGGAAAGGGAGTACTGACTTCCCCTTTTAAGCTATTGAAATCCAATCACCTGGGAGTTGTTCTTACATTTGCTGTGTATCATATGGACTTGCCTCCAGATGCCACATCAGAGCAACGAGTTCAAGCGACTGTGGGGTATTTAGGTGCATCTTACGATGTCCCATCACTGGTGGAGAAGCTCCTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATGCATCTTCTCTGATCAACATGTATGGAACTGATGTTATTGATACTGGGCTGTTGCACATTAGCAGCCTTGATTTTGGTGATCCACAACGCAAACATGAAATGCACTGCAGGTTTAAGCACAAACCTCCTTTTCCTTGGACAGCAGTAAGTGCGGCAGTGGGAGTCCTAGTCATTACCTTGCTAGTTGGTCATATTTTTCATGCGGCCATAAGCCGAATAGCAAAGGTAGAGGCAGACTATTGTGAGATGGAACAACTCAAAGTTCGTGCAGAAGCTGCAGATGTGGCCAAATCACAGTTCCTTGCGACTGTTTCCCATGAAATCCGGACACCAATGAATGGTGTTTTAGGTATGCTGCAAATGCTGATGGACACAAATCTTGATGCCAACCAACAGGATTATGCTGAGACTGCGCATGCTAGTGGAAGAGATCTCATATCGCTGATAAATGAGGTTCTTGATCAGGCCAAAATAGAATCAGGCCGGCTGGAACTTGAAACTGTACCTTTTGATCTTCGTTCGGTGCTTGATAATGTTCTATCACTTTTCTCAGGAAAAACTAATGAGAAAGGGATTGAGTTGGCTGTCTATGTCTCCAATATGGTCCCTGAAGTTGTCATCGGAGACCCAGGACGCCTTCGGCAGATTATTACAAACCTTGTTGGGAATTCAATCAAGTTCACCCATGACAGAGGACATATATTTGTCTCGGTGCATCTGGCGGATGAAGTGAAGGGTCCACCTGATTTTATGGATGAAGTTCTAAGACAAGGCTTGAATTTAGTTGGAGACCTCTCAAACAAAACTTATAATACATTGAGTGGCTTTCCTGTGGTTGATAGATGGAAAAGTTGGGAACGTTTTAAAACATTAAGCAGCACAACAATGGAGGAACCTGATATGATAAAATTAATTGTAACAGTTGAGGATACAGGTGTAGGAATTCCACTAGATGCACAAAGTCGCATCTTCACGCCTTTTATGCAGGCCGATAGTTCCACTTCTAGAACTTATGGTGGTACTGGAATAGGATTGAGCATTAGCAAACGTTTGGTCGATCTCATGTGTGGGGAGATAGGGTTTGTGAGTGAACCTGGCATTGGCAGTACCTTTTCATTTACTGGGTCGTTTCTGAGAGGGAAGACAAGTTCTCTAGATATGAAGTGGCCTCAGTATGAACCAGCTGTCTCAGAATTTCGAGGACTGAGAGCACTGGTAATAGATAAGAGAATCACCCGAACTGAAGTCACAAGATATCATATGCAGAGGTTGGGAATATCGGCGGATATTGCTTCCAGTCTTGAATCAGCATGCTCTTATCTATCTAGTACGAGCAAGACAAGCCTATCCACATGTTTGGCCATGGTTCTTATCGACAAAGATGTTTGGGATAAAGAAACTGGTGTTATGTTCCATCAGTCAGTTAAAGAGCACAGGCAAAACAGCAGCGTGGAGTCTCCCGTGAACTTTCCAAAGATATTTCTCCTGGCTACCTCCATTAGCTCTACTGAACGTAATGAACTAAAATCTTCTGGTCTTGTAGATAATGTATTGATGAAGCCTCTTCGGTTAAGTGTCTTAATTGCCTGCTTCCAAGAGGCCCTTGTAAGCGGTAAAACGAGGCTTGTGAACATAAAAAAACCAACTCTTGGAAAGTTACTACAAGGCAGGAAGATTTTGGTAGTGGATGATAATGCAGTCAACAGAAGAGTCGCAGAAGGTGCTCTAAAGAAATATGGAGCAATTGTCACCTGTGTTGATAGTGGAAAGACTGCTTTAGACATGCTTAAGCCTCCACACAACTTTGACGCTTGCTTTATGGACCTCCAAATGCCAGAAATGGACGGATTTGAAGCAACTCGTCGAATTCGCTGTCTGGAGAGTGAGGTTAATGAGAAAATTGCATCTGGGGAAGCACCAATCGAGATGTTTGGCAATGTGGAGAATTGGCACACACCAATATTAGCTATGACGGCTGATGTGATTCAGGCTTCAAATGAAGAATGCATGAATTGTGGGATGGATGGCTATGTGTCAAAGCCATTTGAAGAAGAACAATTATATACAGCAGTAGCTAAATTCTTCGAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04724 ko04727 ko04974 map04724 map04727 map04974
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

1243

Amino Acids

138.88

Weight (kDa)

6.2

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 359 - 553 1.5e-34 CHASE domain
HisKA PF00512 641 - 706 9.3e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 848 - 919 2.4e-17 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 942 - 1080 2.4e-37 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 1104 - 1236 1.4e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000216)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38860 AT5G38860
fragaria_vesca FvH4_2g24261 FvH4_2g24270 FvH4_3g35490 FvH4_6g03181 FvH4_6g03182 FvH4_6g03221 FvH4_6g03380 FvH4_6g05203 FvH4_6g31981 FvH4_6g32061 FvH4_6g37041 FvH4_6g39371 FvH4_6g45291 FvH4_6g45890 FvH4_6g46240 FvH4_6g46241 FvH4_6g46242 FvH4_6g46260 FvH4_6g46280 FvH4_6g46280 FvH4_6g46280 FvH4_6g46290 FvH4_6g46290 FvH4_6g46380
malus_domestica MD02G1008700.v1.1 MD02G1008900.v1.1 MD04G1180900.v1.1 MD04G1181200.v1.1 MD09G1075900.v1.1 MD09G1201600.v1.1 MD15G1017800.v1.1 MD17G1066600.v1.1
prunus_persica Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509700_v2.0.a1 Prupe.1G510700_v2.0.a1 Prupe.1G510700_v2.0.a1 Prupe.1G512600_v2.0.a1 Prupe.3G059500_v2.0.a1 Prupe.3G059600_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.6G076400_v2.0.a1
pyrus_communis pycom02g00750 pycom03g20320 pycom09g00330 pycom11g08760 pycom17g04880 pycom17g06710 pycom17g19130
rosa_chinensis RchiOBHm_Chr2g0123691 RchiOBHm_Chr2g0164671 RchiOBHm_Chr2g0164681 RchiOBHm_Chr2g0164721 RchiOBHm_Chr2g0164741 RchiOBHm_Chr6g0275871 RchiOBHm_Chr6g0276071 RchiOBHm_Chr6g0291931 RchiOBHm_Chr6g0292091 RchiOBHm_Chr6g0292271 RchiOBHm_Chr7g0221261
rosa_laevigata RLG00000002165 RLG00000007725 RLG00000012062 RLG00000012543 RLG00000013427 RLG00000021441 RLG00000021477 RLG00000021478 RLG00000021480 RLG00000021481
rosa_multiflora Rmu_co8220318.1_g000001 Rmu_co8427407.1_g000001 Rmu_sc0000946.1_g000006 Rmu_sc0000946.1_g000007 Rmu_sc0000946.1_g000010 Rmu_sc0000946.1_g000012 Rmu_sc0000946.1_g000013 Rmu_sc0002030.1_g000015 Rmu_sc0002870.1_g000002 Rmu_sc0003200.1_g000004 Rmu_sc0004915.1_g000002 Rmu_sc0004991.1_g000006 Rmu_sc0006928.1_g000020 Rmu_sc0007384.1_g000001 Rmu_sc0030722.1_g000001 Rmu_sc0030723.1_g000001 Rmu_ssc0000076.1_g000019 Rmu_ssc0000076.1_g000025 Rmu_ssc0000318.1_g000001
rosa_roxburghii Rroxscaffold_2G00086340 Rroxscaffold_2G00086360 Rroxscaffold_2G00086370 Rroxscaffold_2G00086380 Rroxscaffold_2G00086830 Rroxscaffold_2G00087380 Rroxscaffold_2G00120420 Rroxscaffold_7G00175260 Rroxscaffold_7G00175600 Rroxscaffold_7G00177970 Rroxscaffold_7G00192830
rosa_rugosa Rorug02G0247400 Rorug02G0506200 Rorug02G0508300 Rorug02G0509700 Rorug05G0378800 Rorug06G0096600 Rorug06G0096700 Rorug06G0221800
rosa_samantha Rh2AG577600 Rh2AG577700 Rh2AG577800 Rh2AG578000 Rh2AG578100 Rh2BG589200 Rh2CG559800 Rh2DG594400 Rh2DG594500 Rh2DG597800 Rh2DG599400 Rh2DG599500 Rh2DG599600 Rh6AG338400 Rh6BG211200 Rh6BG212600 Rh6BG342900 Rh6BG345600 Rh6CG214800 Rh6CG349100 Rh6CG350600 Rh6CG350700 Rh6CG352600 Rh7AG340800
rosa_wichuraiana Rw1G028590 Rw2G047980 Rw2G047990 Rw5G043360 Rw6G018180 Rw6G029200 Rw6G029450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2445
Acc16I TGCGCA 1 cut(s) 2049
Acc36I ACCTGC 2 cut(s) 52, 1734
AccB7I CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3284
AccI GTMKAC 1 cut(s) 542
AccII CGCG 1 cut(s) 1371
AccIII TCCGGA 1 cut(s) 1959
AciI CCGC 7 cut(s) 536, 1369, 1787, 1838, 2357, 2918, 3246
AclI AACGTT 2 cut(s) 2494, 2683
AclWI GGATC 4 cut(s) 592, 1265, 1278, 1709
AcoI YGGCCR 3 cut(s) 1839, 1920, 2994
AcsI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2577, 2828, 3501, 3713
AcuI CTGAAG 2 cut(s) 2265, 2898
AcvI CACGTG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 2269
AfaI GTAC 5 cut(s) 1410, 2139, 2656, 2743, 2965
AfiI CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
AflII CTTAAG 1 cut(s) 3422
AflIII ACRYGT 3 cut(s) 963, 1653, 2987
AhdI GACNNNNNGTC 1 cut(s) 1351
AhlI ACTAGT 1 cut(s) 1136
AjnI CCWGG 7 cut(s) 165, 462, 485, 1446, 2263, 2728, 3120
AjuI GAANNNNNNNTTGG 8 cut(s) 450, 482, 1239, 1271, 1916, 1948, 2718, 2750
AleI CACNNNNGTG 1 cut(s) 611
AloI GAACNNNNNNTCC 2 cut(s) 2767, 2799
Alw21I GWGCWC 4 cut(s) 608, 2848, 3069, 3361
Alw44I GTGCAC 1 cut(s) 604
AlwI GGATC 4 cut(s) 592, 1265, 1278, 1709
AlwNI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
Aor13HI TCCGGA 1 cut(s) 1959
AoxI GGCC 9 cut(s) 86, 1839, 1920, 2100, 2116, 2622, 2801, 2994, 3234
ApaLI GTGCAC 1 cut(s) 604
ApeKI GCWGC 9 cut(s) 241, 244, 775, 796, 1698, 1910, 1990, 2511, 3081
ApoI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2577, 2828, 3501, 3713
ArsI GACNNNNNNTTYG 4 cut(s) 2673, 2705, 3493, 3525
AseI ATTAAT 1 cut(s) 2546
AspLEI GCGC 2 cut(s) 1300, 2050
AspS9I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
AsuC2I CCSGG 1 cut(s) 982
AsuHPI GGTGA 7 cut(s) 1046, 1109, 1436, 1724, 2308, 2860, 3376
AvaII GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 2 cut(s) 608, 749
BaeI ACNNNNGTAYC 2 cut(s) 2556, 2589
BalI TGGCCA 2 cut(s) 1922, 2996
BamHI GGATCC 1 cut(s) 1270
BanII GRGCYC 2 cut(s) 78, 982
BbrPI CACGTG 1 cut(s) 849
BbsI GAAGAC 2 cut(s) 209, 2783
Bbv12I GWGCWC 4 cut(s) 608, 2848, 3069, 3361
BbvI GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2523, 3093
BceAI ACGGC 1 cut(s) 3624
BciT130I CCWGG 7 cut(s) 167, 464, 487, 1448, 2265, 2730, 3122
BciVI GTATCC 1 cut(s) 2557
BclI TGATCA 3 cut(s) 1081, 1647, 2095
BcnI CCSGG 1 cut(s) 982
BcuI ACTAGT 1 cut(s) 1136
BfaI CTAG 8 cut(s) 1137, 1802, 1817, 2055, 2585, 2640, 2789, 2961
BfmI CTRYAG 4 cut(s) 509, 543, 1741, 1911
BfrI CTTAAG 1 cut(s) 3422
BfuAI ACCTGC 2 cut(s) 52, 1734
BfuI GTATCC 1 cut(s) 2557
BglII AGATCT 1 cut(s) 2065
BmcAI AGTACT 1 cut(s) 1410
Bme1390I CCNGG 8 cut(s) 167, 464, 487, 982, 1448, 2265, 2730, 3122
Bme18I GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
BmeRI GACNNNNNGTC 1 cut(s) 1351
BmgT120I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
BmiI GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
BmrFI CCNGG 8 cut(s) 167, 464, 487, 982, 1448, 2265, 2730, 3122
BmrI ACTGGG 2 cut(s) 1689, 2766
BmuI ACTGGG 2 cut(s) 1689, 2766
BoxI GACNNNNGTC 1 cut(s) 1241
BpiI GAAGAC 2 cut(s) 209, 2783
BpmI CTGGAG 3 cut(s) 192, 1476, 3533
BpuEI CTTGAG 1 cut(s) 1299
BpuMI CCSGG 1 cut(s) 982
BsaAI YACGTR 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 2269
BsaI GGTCTC 3 cut(s) 1120, 2253, 2418
BsaWI WCCGGW 1 cut(s) 1959
BsaXI ACNNNNNCTCC 6 cut(s) 1788, 1818, 2699, 2729, 3366, 3396
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
Bse118I RCCGGY 1 cut(s) 2118
Bse1I ACTGG 7 cut(s) 1569, 1684, 2662, 2761, 2853, 2931, 3040
Bse21I CCTNAGG 1 cut(s) 834
Bse3DI GCAATG 2 cut(s) 75, 3577
BseAI TCCGGA 1 cut(s) 1959
BseBI CCWGG 7 cut(s) 167, 464, 487, 1448, 2265, 2730, 3122
BseLI CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
BseMI GCAATG 2 cut(s) 75, 3577
BseMII CTCAG 9 cut(s) 635, 1164, 1218, 2031, 2199, 2759, 2819, 2831, 2838
BseNI ACTGG 7 cut(s) 1569, 1684, 2662, 2761, 2853, 2931, 3040
BseSI GKGCMC 2 cut(s) 608, 749
BseXI GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2523, 3093
BsgI GTGCAG 2 cut(s) 1565, 1923
Bsh1236I CGCG 1 cut(s) 1371
BshFI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
BsiHKAI GWGCWC 4 cut(s) 608, 2848, 3069, 3361
BsiSI CCGG 3 cut(s) 982, 1960, 2119
BslFI GGGAC 3 cut(s) 195, 1541, 2225
BslI CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
BsmBI CGTCTC 1 cut(s) 1048
BsmFI GGGAC 3 cut(s) 195, 1541, 2225
BsmI GAATGC 1 cut(s) 3644
BsnI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
Bso31I GGTCTC 3 cut(s) 1120, 2253, 2418
Bsp1286I GDGCHC 7 cut(s) 78, 608, 749, 982, 2848, 3069, 3361
Bsp13I TCCGGA 1 cut(s) 1959
Bsp19I CCATGG 2 cut(s) 1057, 2997
BspACI CCGC 7 cut(s) 536, 1369, 1787, 1838, 2357, 2918, 3246
BspANI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
BspCNI CTCAG 9 cut(s) 634, 1165, 1219, 2032, 2198, 2760, 2818, 2832, 2837
BspEI TCCGGA 1 cut(s) 1959
BspFNI CGCG 1 cut(s) 1371
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
BspMAI CTGCAG 2 cut(s) 1745, 1915
BspMI ACCTGC 2 cut(s) 52, 1734
BspPI GGATC 4 cut(s) 592, 1265, 1278, 1709
BspTI CTTAAG 1 cut(s) 3422
BspTNI GGTCTC 3 cut(s) 1120, 2253, 2418
BsrDI GCAATG 2 cut(s) 75, 3577
BsrFI RCCGGY 1 cut(s) 2118
BsrI ACTGG 7 cut(s) 1569, 1684, 2662, 2761, 2853, 2931, 3040
BssAI RCCGGY 1 cut(s) 2118
BssNI GRCGYC 1 cut(s) 2269
BssT1I CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2997
Bst2UI CCWGG 7 cut(s) 167, 464, 487, 1448, 2265, 2730, 3122
Bst4CI ACNGT 9 cut(s) 151, 547, 827, 1324, 1528, 1932, 1945, 2137, 2557
Bst6I CTCTTC 3 cut(s) 2056, 3204, 3337
BstACI GRCGYC 1 cut(s) 2269
BstAFI CTTAAG 1 cut(s) 3422
BstAPI GCANNNNNTGC 3 cut(s) 1784, 1910, 3356
BstBAI YACGTR 1 cut(s) 849
BstDSI CCRYGG 2 cut(s) 1057, 2997
BstFNI CGCG 1 cut(s) 1371
BstHHI GCGC 2 cut(s) 1300, 2050
BstNI CCWGG 7 cut(s) 167, 464, 487, 1448, 2265, 2730, 3122
BstNSI RCATGY 6 cut(s) 967, 1657, 2054, 2949, 2991, 3421
BstPAI GACNNNNGTC 1 cut(s) 1241
BstSCI CCNGG 8 cut(s) 165, 462, 485, 980, 1446, 2263, 2728, 3120
BstSFI CTRYAG 4 cut(s) 509, 543, 1741, 1911
BstSLI GKGCMC 2 cut(s) 608, 749
BstUI CGCG 1 cut(s) 1371
BstV1I GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2523, 3093
BstV2I GAAGAC 2 cut(s) 209, 2783
BstX2I RGATCY 2 cut(s) 1270, 2065
BstXI CCANNNNNNTGG 3 cut(s) 692, 1447, 2992
BstYI RGATCY 2 cut(s) 1270, 2065
Bsu36I CCTNAGG 1 cut(s) 834
BsuI GTATCC 1 cut(s) 2557
BsuRI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
BtgI CCRYGG 2 cut(s) 1057, 2997
BtsI GCAGTG 2 cut(s) 1738, 1797
BtsIMutI CAGTG 5 cut(s) 510, 1562, 1738, 1797, 2846
BveI ACCTGC 2 cut(s) 52, 1734
CaiI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
CciI TCATGA 1 cut(s) 739
CfoI GCGC 2 cut(s) 1300, 2050
Cfr10I RCCGGY 1 cut(s) 2118
Cfr13I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
CseI GACGC 2 cut(s) 2277, 3452
Csp6I GTAC 5 cut(s) 1409, 2138, 2655, 2742, 2964
CviQI GTAC 5 cut(s) 1409, 2138, 2655, 2742, 2964
DraI TTTAAA 2 cut(s) 1158, 2500
DriI GACNNNNNGTC 1 cut(s) 1351
EaeI YGGCCR 3 cut(s) 1839, 1920, 2994
Eam1104I CTCTTC 3 cut(s) 2056, 3204, 3337
Eam1105I GACNNNNNGTC 1 cut(s) 1351
EarI CTCTTC 3 cut(s) 2056, 3204, 3337
EciI GGCGGA 2 cut(s) 2372, 2933
Eco130I CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2997
Eco24I GRGCYC 2 cut(s) 78, 982
Eco31I GGTCTC 3 cut(s) 1120, 2253, 2418
Eco32I GATATC 1 cut(s) 2891
Eco47I GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
Eco57I CTGAAG 2 cut(s) 2265, 2898
Eco72I CACGTG 1 cut(s) 849
Eco81I CCTNAGG 1 cut(s) 834
EcoO109I RGGNCCY 1 cut(s) 3235
EcoRI GAATTC 3 cut(s) 2302, 2577, 3501
EcoRII CCWGG 7 cut(s) 165, 462, 485, 1446, 2263, 2728, 3120
EcoRV GATATC 1 cut(s) 2891
EcoT14I CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2997
EcoT22I ATGCAT 2 cut(s) 1639, 3644
EcoT38I GRGCYC 2 cut(s) 78, 982
ErhI CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2997
Esp3I CGTCTC 1 cut(s) 1048
FalI AAGNNNNNCTT 6 cut(s) 939, 971, 1239, 1271, 3222, 3254
FaqI GGGAC 3 cut(s) 195, 1541, 2225
FauNDI CATATG 2 cut(s) 1479, 2895
FbaI TGATCA 3 cut(s) 1081, 1647, 2095
FblI GTMKAC 1 cut(s) 542
FriOI GRGCYC 2 cut(s) 78, 982
FspBI CTAG 8 cut(s) 1137, 1802, 1817, 2055, 2585, 2640, 2789, 2961
FspI TGCGCA 1 cut(s) 2049
GlaI GCGC 2 cut(s) 1299, 2049
GsuI CTGGAG 3 cut(s) 192, 1476, 3533
HaeIII GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
HapII CCGG 3 cut(s) 982, 1960, 2119
HgaI GACGC 2 cut(s) 2277, 3452
HhaI GCGC 2 cut(s) 1300, 2050
Hin1I GRCGYC 1 cut(s) 2269
Hin6I GCGC 2 cut(s) 1298, 2048
HinP1I GCGC 2 cut(s) 1298, 2048
HincII GTYRAC 2 cut(s) 1395, 3337
HindII GTYRAC 2 cut(s) 1395, 3337
HindIII AAGCTT 2 cut(s) 116, 1264
HpaII CCGG 3 cut(s) 982, 1960, 2119
HphI GGTGA 7 cut(s) 1046, 1109, 1436, 1724, 2308, 2860, 3376
Hpy99I CGWCG 1 cut(s) 3501
HpyAV CCTTC 6 cut(s) 376, 1080, 1186, 2282, 2362, 3347
HpyCH4III ACNGT 9 cut(s) 151, 547, 827, 1324, 1528, 1932, 1945, 2137, 2557
HpyCH4IV ACGT 5 cut(s) 848, 896, 2494, 2683, 3148
HpySE526I ACGT 5 cut(s) 848, 896, 2494, 2683, 3148
Hsp92I GRCGYC 1 cut(s) 2269
HspAI GCGC 2 cut(s) 1298, 2048
Kpn2I TCCGGA 1 cut(s) 1959
KroI GCCGGC 1 cut(s) 2118
KroNI GCCGGC 1 cut(s) 2120
Ksp22I TGATCA 3 cut(s) 1081, 1647, 2095
LmnI GCTCC 3 cut(s) 73, 1581, 3374
Lsp1109I GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2523, 3093
MaeI CTAG 8 cut(s) 1137, 1802, 1817, 2055, 2585, 2640, 2789, 2961
MaeII ACGT 5 cut(s) 848, 896, 2494, 2683, 3148
MaeIII GTNAC 6 cut(s) 326, 1162, 2551, 2881, 3291, 3382
MfeI CAATTG 3 cut(s) 686, 1605, 3378
MflI RGATCY 2 cut(s) 1270, 2065
MhlI GDGCHC 7 cut(s) 78, 608, 749, 982, 2848, 3069, 3361
MlsI TGGCCA 2 cut(s) 1922, 2996
MluNI TGGCCA 2 cut(s) 1922, 2996
MlyI GAGTC 6 cut(s) 199, 1231, 1806, 3098, 3354, 3731
MmeI TCCRAC 3 cut(s) 646, 984, 2401
Mox20I TGGCCA 2 cut(s) 1922, 2996
Mph1103I ATGCAT 2 cut(s) 1639, 3644
MroI TCCGGA 1 cut(s) 1959
MroNI GCCGGC 1 cut(s) 2118
MscI TGGCCA 2 cut(s) 1922, 2996
MslI CAYNNNNRTG 6 cut(s) 252, 611, 921, 1023, 2055, 2519
Msp20I TGGCCA 2 cut(s) 1922, 2996
MspA1I CMGCKG 1 cut(s) 2819
MspCI CTTAAG 1 cut(s) 3422
MspI CCGG 3 cut(s) 982, 1960, 2119
MspR9I CCNGG 8 cut(s) 167, 464, 487, 982, 1448, 2265, 2730, 3122
MunI CAATTG 3 cut(s) 686, 1605, 3378
Mva1269I GAATGC 1 cut(s) 3644
MvaI CCWGG 7 cut(s) 167, 464, 487, 1448, 2265, 2730, 3122
MvnI CGCG 1 cut(s) 1371
NaeI GCCGGC 1 cut(s) 2120
NciI CCSGG 1 cut(s) 982
NcoI CCATGG 2 cut(s) 1057, 2997
NdeI CATATG 2 cut(s) 1479, 2895
NgoMIV GCCGGC 1 cut(s) 2118
NlaIV GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
NmuCI GTSAC 2 cut(s) 2881, 3382
NsbI TGCGCA 1 cut(s) 2049
NsiI ATGCAT 2 cut(s) 1639, 3644
NspI RCATGY 6 cut(s) 967, 1657, 2054, 2949, 2991, 3421
OliI CACNNNNGTG 1 cut(s) 611
PaeI GCATGC 2 cut(s) 2054, 2949
PagI TCATGA 1 cut(s) 739
PciI ACATGT 3 cut(s) 963, 1653, 2987
PctI GAATGC 1 cut(s) 3644
PdiI GCCGGC 1 cut(s) 2120
PfeI GAWTC 7 cut(s) 234, 299, 617, 2111, 2865, 2939, 3619
PflMI CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3284
PleI GAGTC 6 cut(s) 199, 1231, 1805, 3097, 3353, 3730
PmaCI CACGTG 1 cut(s) 849
PmlI CACGTG 1 cut(s) 849
PpsI GAGTC 6 cut(s) 199, 1231, 1805, 3097, 3353, 3730
Ppu21I YACGTR 1 cut(s) 849
PscI ACATGT 3 cut(s) 963, 1653, 2987
PshAI GACNNNNGTC 1 cut(s) 1241
PshBI ATTAAT 1 cut(s) 2546
PsiI TTATAA 1 cut(s) 2445
Psp1406I AACGTT 2 cut(s) 2494, 2683
Psp6I CCWGG 7 cut(s) 165, 462, 485, 1446, 2263, 2728, 3120
PspCI CACGTG 1 cut(s) 849
PspGI CCWGG 7 cut(s) 165, 462, 485, 1446, 2263, 2728, 3120
PspN4I GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
PspPI GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
PstI CTGCAG 2 cut(s) 1745, 1915
PstNI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
PsuI RGATCY 2 cut(s) 1270, 2065
PvuII CAGCTG 1 cut(s) 2819
RsaI GTAC 5 cut(s) 1410, 2139, 2656, 2743, 2965
RsaNI GTAC 5 cut(s) 1409, 2138, 2655, 2742, 2964
RseI CAYNNNNRTG 6 cut(s) 252, 611, 921, 1023, 2055, 2519
Sau96I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
ScaI AGTACT 1 cut(s) 1410
SchI GAGTC 6 cut(s) 199, 1231, 1806, 3098, 3354, 3731
ScrFI CCNGG 8 cut(s) 167, 464, 487, 982, 1448, 2265, 2730, 3122
SduI GDGCHC 7 cut(s) 78, 608, 749, 982, 2848, 3069, 3361
SfcI CTRYAG 4 cut(s) 509, 543, 1741, 1911
SinI GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
SmiMI CAYNNNNRTG 6 cut(s) 252, 611, 921, 1023, 2055, 2519
SmlI CTYRAG 2 cut(s) 1314, 3422
SmoI CTYRAG 2 cut(s) 1314, 3422
SpeI ACTAGT 1 cut(s) 1136
SphI GCATGC 2 cut(s) 2054, 2949
SsiI CCGC 7 cut(s) 536, 1369, 1787, 1838, 2357, 2918, 3246
SspI AATATT 1 cut(s) 3597
SspMI CTAG 8 cut(s) 1137, 1802, 1817, 2055, 2585, 2640, 2789, 2961
StyD4I CCNGG 8 cut(s) 165, 462, 485, 980, 1446, 2263, 2728, 3120
StyI CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2997
TaaI ACNGT 9 cut(s) 151, 547, 827, 1324, 1528, 1932, 1945, 2137, 2557
TaiI ACGT 5 cut(s) 851, 899, 2497, 2686, 3151
TaqI TCGA 9 cut(s) 107, 232, 1340, 2691, 2833, 3009, 3499, 3558, 3720
TatI WGTACW 1 cut(s) 1408
TauI GCSGC 2 cut(s) 1790, 1841
TfiI GAWTC 7 cut(s) 234, 299, 617, 2111, 2865, 2939, 3619
TscAI CASTG 5 cut(s) 510, 1569, 1745, 1797, 2853
TseFI GTSAC 2 cut(s) 2881, 3382
TseI GCWGC 9 cut(s) 241, 244, 775, 796, 1698, 1910, 1990, 2511, 3081
Tsp45I GTSAC 2 cut(s) 2881, 3382
TspGWI ACGGA 1 cut(s) 3495
TspRI CASTG 5 cut(s) 510, 1569, 1745, 1797, 2853
Van91I CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3284
Vha464I CTTAAG 1 cut(s) 3422
VneI GTGCAC 1 cut(s) 604
VpaK11BI GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
VspI ATTAAT 1 cut(s) 2546
XapI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2577, 2828, 3501, 3713
XbaI TCTAGA 2 cut(s) 2639, 2788
XceI RCATGY 6 cut(s) 967, 1657, 2054, 2949, 2991, 3421
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 8 cut(s) 1137, 1802, 1817, 2055, 2585, 2640, 2789, 2961
ZrmI AGTACT 1 cut(s) 1410
Zsp2I ATGCAT 2 cut(s) 1639, 3644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.