pycom03g20320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000216
Physical Location & Seq
Reverse (-)
20845941 .. 20847332
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20320.2

Sequence Viewer

Length: 1314 bp
ATGTATATTGACGATCTTCCTCATCATCTCTTGGTTGAAGTTCTTGGTCGACTTCCATATAAAATTGTTATTCAGTGCAAGTGCGTGTCCAAGCAATGGCTCTCTGTCATCTCCGATCCATCTTTTATTCACCGCTTTGTACGTCAACAAGGACATACCGATTCAGATACGATATTATTAGATATCAGGACACAGGTCCTTAGGGATGTATTATTATACACCGTGACGTCGATTTCGGACGAGTTCAAAACGCGCGAGATTTCTTTCACTTGGGGACAATGTTCTCTAAGTTTATTCCCTTGGACGCAACGTGTTGTAGCGACGCATAACGATTTAATCTTGTGCTGTCGATTCCGAAGCTTTGAGCTACGCGCGGGGCTCATATGCGACAATCCTTACTATAACCCTACTGAAGATGGCTGCACTACGGTCAAATTTAATACGGAGTACAGGTACAGGGTTGTGCGAATCCTTCGAAAATGTGACGACCTAAGCTCATTCACAGTTGAGATGTCCTCGTCTGAGACTGGTGAATGGAGAGAGATAGTTATATTATGCTCCCCAGGCTCTAAGTTCGTTTGTGTCCATCACCCGTGTATTGCTTTAAATGGAATGTTGTATTGGATGGACCACAATGGCAGGCTTTTCGGGTTTGATCCATTCAACAACATGAACAGCACAATCCGTGCTGCCGCTAAATTTGTTGACGACAACGAATTATGTCGTTTCATTGAATTTGGTGATTCCCCTTACATTATGGACGCGTTATGTTCTTATACTCTAGGGGTGTGTCGAGGGCGTCTGCGGCTGTTCATTCAAGATGATTCCGGCGAAAATTTGTTTATTTGGGAGTGGAAAGAAGTAGAAAAACAGCAGGGCAATATTAATGGAGTCAAACTGATGGAGTGGTGTTTGATAAAAAGGATTTCCTGGGAAGAAATGGTTCCAAAAGATAAACCAGCGATTGCGAAATGGCTAAAGGAAGAGAAGTGGTCTTGGACTAGGGTGCTGGTTGGTGATCCAAACGATGGGGATGTTTTATATCTGCATCGTAAGCATGATATTTTACCATGCAACGTTCGTACAAAAACTTGGCTATGGAGAACGGAGCCGCAAAGTCCCAATAATTACAGATCGTTTCAATTTTCACTTGATGTTGGCCGACACCAATTCCTAGACTACATTATAGTTGATTTATTAACATGTTTTTTGGATGATATTTGTTTTCTAAGTTATCTTAGTAGTTGTTATCTCATCATAATTGAAGAACAAGATAGCTTGTTGAATTATGATGGACTTATTTCATGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

438

Amino Acids

51.17

Weight (kDa)

5.62

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000216)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38860 AT5G38860
fragaria_vesca FvH4_2g24261 FvH4_2g24270 FvH4_3g35490 FvH4_6g03181 FvH4_6g03182 FvH4_6g03221 FvH4_6g03380 FvH4_6g05203 FvH4_6g31981 FvH4_6g32061 FvH4_6g37041 FvH4_6g39371 FvH4_6g45291 FvH4_6g45890 FvH4_6g46240 FvH4_6g46241 FvH4_6g46242 FvH4_6g46260 FvH4_6g46280 FvH4_6g46280 FvH4_6g46280 FvH4_6g46290 FvH4_6g46290 FvH4_6g46380
malus_domestica MD02G1008700.v1.1 MD02G1008900.v1.1 MD04G1180900.v1.1 MD04G1181200.v1.1 MD09G1075900.v1.1 MD09G1201600.v1.1 MD15G1017800.v1.1 MD17G1066600.v1.1
prunus_persica Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509300_v2.0.a1 Prupe.1G509700_v2.0.a1 Prupe.1G510700_v2.0.a1 Prupe.1G510700_v2.0.a1 Prupe.1G512600_v2.0.a1 Prupe.3G059500_v2.0.a1 Prupe.3G059600_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.3G248100_v2.0.a1 Prupe.6G076400_v2.0.a1
pyrus_communis pycom02g00750 pycom03g20320 pycom09g00330 pycom11g08760 pycom17g04880 pycom17g06710 pycom17g19130
rosa_chinensis RchiOBHm_Chr2g0123691 RchiOBHm_Chr2g0164671 RchiOBHm_Chr2g0164681 RchiOBHm_Chr2g0164721 RchiOBHm_Chr2g0164741 RchiOBHm_Chr6g0275871 RchiOBHm_Chr6g0276071 RchiOBHm_Chr6g0291931 RchiOBHm_Chr6g0292091 RchiOBHm_Chr6g0292271 RchiOBHm_Chr7g0221261
rosa_laevigata RLG00000002165 RLG00000007725 RLG00000012062 RLG00000012543 RLG00000013427 RLG00000021441 RLG00000021477 RLG00000021478 RLG00000021480 RLG00000021481
rosa_multiflora Rmu_co8220318.1_g000001 Rmu_co8427407.1_g000001 Rmu_sc0000946.1_g000006 Rmu_sc0000946.1_g000007 Rmu_sc0000946.1_g000010 Rmu_sc0000946.1_g000012 Rmu_sc0000946.1_g000013 Rmu_sc0002030.1_g000015 Rmu_sc0002870.1_g000002 Rmu_sc0003200.1_g000004 Rmu_sc0004915.1_g000002 Rmu_sc0004991.1_g000006 Rmu_sc0006928.1_g000020 Rmu_sc0007384.1_g000001 Rmu_sc0030722.1_g000001 Rmu_sc0030723.1_g000001 Rmu_ssc0000076.1_g000019 Rmu_ssc0000076.1_g000025 Rmu_ssc0000318.1_g000001
rosa_roxburghii Rroxscaffold_2G00086340 Rroxscaffold_2G00086360 Rroxscaffold_2G00086370 Rroxscaffold_2G00086380 Rroxscaffold_2G00086830 Rroxscaffold_2G00087380 Rroxscaffold_2G00120420 Rroxscaffold_7G00175260 Rroxscaffold_7G00175600 Rroxscaffold_7G00177970 Rroxscaffold_7G00192830
rosa_rugosa Rorug02G0247400 Rorug02G0506200 Rorug02G0508300 Rorug02G0509700 Rorug05G0378800 Rorug06G0096600 Rorug06G0096700 Rorug06G0221800
rosa_samantha Rh2AG577600 Rh2AG577700 Rh2AG577800 Rh2AG578000 Rh2AG578100 Rh2BG589200 Rh2CG559800 Rh2DG594400 Rh2DG594500 Rh2DG597800 Rh2DG599400 Rh2DG599500 Rh2DG599600 Rh6AG338400 Rh6BG211200 Rh6BG212600 Rh6BG342900 Rh6BG345600 Rh6CG214800 Rh6CG349100 Rh6CG350600 Rh6CG350700 Rh6CG352600 Rh7AG340800
rosa_wichuraiana Rw1G028590 Rw2G047980 Rw2G047990 Rw5G043360 Rw6G018180 Rw6G029200 Rw6G029450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 230
AccB7I CCANNNNNTGG 2 cut(s) 96, 1028
AccI GTMKAC 1 cut(s) 49
AccII CGCG 5 cut(s) 253, 255, 372, 374, 764
AciI CCGC 5 cut(s) 133, 374, 693, 805, 1112
AclI AACGTT 1 cut(s) 1077
AclWI GGATC 3 cut(s) 110, 650, 1013
AcoI YGGCCR 1 cut(s) 1159
AcsI RAATTY 4 cut(s) 434, 698, 734, 835
AcuI CTGAAG 1 cut(s) 432
AcyI GRCGYC 2 cut(s) 227, 799
AfaI GTAC 4 cut(s) 141, 449, 455, 1084
AfiI CCNNNNNNNGG 2 cut(s) 96, 1028
AflIII ACRYGT 3 cut(s) 310, 762, 1202
AgsI TTSAA 8 cut(s) 38, 247, 664, 734, 818, 1142, 1265, 1285
AjnI CCWGG 2 cut(s) 562, 929
AjuI GAANNNNNNNTTGG 2 cut(s) 604, 636
AluBI AGCT 4 cut(s) 360, 367, 495, 1278
AluI AGCT 4 cut(s) 360, 367, 495, 1278
Alw26I GTCTC 1 cut(s) 518
AlwI GGATC 3 cut(s) 110, 650, 1013
AoxI GGCC 1 cut(s) 1159
ApeKI GCWGC 2 cut(s) 420, 689
ApoI RAATTY 4 cut(s) 434, 698, 734, 835
ArsI GACNNNNNNTTYG 2 cut(s) 217, 249
AseI ATTAAT 1 cut(s) 885
Asp700I GAANNNNTTC 1 cut(s) 942
AspLEI GCGC 2 cut(s) 255, 374
AspS9I GGNCC 2 cut(s) 196, 628
AsuHPI GGTGA 5 cut(s) 122, 542, 581, 752, 1028
AsuII TTCGAA 1 cut(s) 475
AvaII GGWCC 2 cut(s) 196, 628
AxyI CCTNAGG 1 cut(s) 200
BanII GRGCYC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 407, 676
BccI CCATC 7 cut(s) 127, 410, 594, 619, 895, 1022, 1286
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 2 cut(s) 564, 931
BcoDI GTCTC 1 cut(s) 518
BfaI CTAG 3 cut(s) 782, 1002, 1175
BisI GCNGC 5 cut(s) 421, 690, 693, 806, 1112
BlsI GCNGC 5 cut(s) 422, 691, 694, 807, 1113
Bme1390I CCNGG 2 cut(s) 564, 931
Bme18I GGWCC 2 cut(s) 196, 628
BmgT120I GGNCC 2 cut(s) 196, 628
BmiI GGNNCC 2 cut(s) 945, 1110
BmrFI CCNGG 2 cut(s) 564, 931
BmsI GCATC 1 cut(s) 1057
BoxI GACNNNNGTC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 500, 532
Bpu10I CCTNAGC 1 cut(s) 491
Bpu14I TTCGAA 1 cut(s) 475
BsaHI GRCGYC 2 cut(s) 227, 799
BsaJI CCNNGG 3 cut(s) 299, 562, 930
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 1028
Bse1I ACTGG 1 cut(s) 532
Bse21I CCTNAGG 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 101
BseBI CCWGG 2 cut(s) 564, 931
BseDI CCNNGG 3 cut(s) 299, 562, 930
BseGI GGATG 4 cut(s) 211, 630, 1039, 1219
BseLI CCNNNNNNNGG 2 cut(s) 96, 1028
BseMI GCAATG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 532
BseXI GCAGC 2 cut(s) 407, 676
BsgI GTGCAG 1 cut(s) 406
Bsh1236I CGCG 5 cut(s) 253, 255, 372, 374, 764
BshFI GGCC 1 cut(s) 1161
BsiSI CCGG 1 cut(s) 828
BslFI GGGAC 2 cut(s) 288, 1104
BslI CCNNNNNNNGG 2 cut(s) 96, 1028
BsmAI GTCTC 1 cut(s) 518
BsmFI GGGAC 2 cut(s) 288, 1104
BsnI GGCC 1 cut(s) 1161
Bsp119I TTCGAA 1 cut(s) 475
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 5 cut(s) 13, 115, 655, 1018, 1133
BspACI CCGC 5 cut(s) 133, 374, 693, 805, 1112
BspANI GGCC 1 cut(s) 1161
BspCNI CTCAG 1 cut(s) 514
BspFNI CGCG 5 cut(s) 253, 255, 372, 374, 764
BspLI GGNNCC 2 cut(s) 945, 1110
BspPI GGATC 3 cut(s) 110, 650, 1013
BspT104I TTCGAA 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 101
BsrI ACTGG 1 cut(s) 532
BssECI CCNNGG 3 cut(s) 299, 562, 930
BssMI GATC 5 cut(s) 13, 115, 655, 1018, 1133
BssNI GRCGYC 2 cut(s) 227, 799
BssT1I CCWWGG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 564, 931
Bst4CI ACNGT 3 cut(s) 223, 430, 505
Bst6I CTCTTC 1 cut(s) 978
BstACI GRCGYC 2 cut(s) 227, 799
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 1 cut(s) 641
BstDEI CTNAG 7 cut(s) 200, 287, 491, 522, 570, 1229, 1238
BstF5I GGATG 4 cut(s) 211, 630, 1039, 1219
BstFNI CGCG 5 cut(s) 253, 255, 372, 374, 764
BstHHI GCGC 2 cut(s) 255, 374
BstKTI GATC 5 cut(s) 16, 118, 658, 1021, 1136
BstMAI GTCTC 1 cut(s) 518
BstMBI GATC 5 cut(s) 13, 115, 655, 1018, 1133
BstMWI GCNNNNNNNGC 3 cut(s) 564, 805, 1054
BstNI CCWGG 2 cut(s) 564, 931
BstNSI RCATGY 1 cut(s) 1206
BstPAI GACNNNNGTC 1 cut(s) 194
BstSCI CCNGG 2 cut(s) 562, 929
BstUI CGCG 5 cut(s) 253, 255, 372, 374, 764
BstV1I GCAGC 2 cut(s) 407, 676
Bsu36I CCTNAGG 1 cut(s) 200
BsuRI GGCC 1 cut(s) 1161
BtsCI GGATG 4 cut(s) 211, 630, 1039, 1219
BtsIMutI CAGTG 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 641
CfoI GCGC 2 cut(s) 255, 374
Cfr13I GGNCC 2 cut(s) 196, 628
CseI GACGC 4 cut(s) 313, 331, 770, 788
Csp6I GTAC 4 cut(s) 140, 448, 454, 1083
CviAII CATG 5 cut(s) 670, 1058, 1071, 1203, 1305
CviQI GTAC 4 cut(s) 140, 448, 454, 1083
DdeI CTNAG 7 cut(s) 200, 287, 491, 522, 570, 1229, 1238
DpnI GATC 5 cut(s) 15, 117, 657, 1020, 1135
DpnII GATC 5 cut(s) 13, 115, 655, 1018, 1133
DraI TTTAAA 1 cut(s) 606
EaeI YGGCCR 1 cut(s) 1159
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
Eco130I CCWWGG 1 cut(s) 299
Eco24I GRGCYC 1 cut(s) 381
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 196, 628
Eco57I CTGAAG 1 cut(s) 432
Eco81I CCTNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 2 cut(s) 562, 929
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 5 cut(s) 673, 1061, 1074, 1206, 1308
FaqI GGGAC 2 cut(s) 288, 1104
FatI CATG 5 cut(s) 669, 1057, 1070, 1202, 1304
FauI CCCGC 1 cut(s) 367
FauNDI CATATG 1 cut(s) 383
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 5 cut(s) 421, 690, 693, 806, 1112
FokI GGATG 4 cut(s) 218, 637, 1046, 1226
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 5 cut(s) 421, 690, 693, 806, 1112
FspBI CTAG 3 cut(s) 782, 1002, 1175
GlaI GCGC 2 cut(s) 254, 373
GluI GCNGC 5 cut(s) 421, 690, 693, 806, 1112
HaeIII GGCC 1 cut(s) 1161
HapII CCGG 1 cut(s) 828
HgaI GACGC 4 cut(s) 313, 331, 770, 788
HhaI GCGC 2 cut(s) 255, 374
Hin1I GRCGYC 2 cut(s) 227, 799
Hin1II CATG 5 cut(s) 673, 1061, 1074, 1206, 1308
Hin6I GCGC 2 cut(s) 253, 372
HinP1I GCGC 2 cut(s) 253, 372
HincII GTYRAC 3 cut(s) 50, 146, 706
HindII GTYRAC 3 cut(s) 50, 146, 706
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 6 cut(s) 161, 351, 468, 743, 824, 891
HpaII CCGG 1 cut(s) 828
HphI GGTGA 5 cut(s) 122, 542, 581, 752, 1028
Hpy166II GTNNAC 3 cut(s) 50, 146, 706
Hpy188I TCNGA 5 cut(s) 115, 166, 238, 356, 523
Hpy188III TCNNGA 2 cut(s) 187, 818
Hpy8I GTNNAC 3 cut(s) 50, 146, 706
Hpy99I CGWCG 2 cut(s) 232, 325
HpyAV CCTTC 1 cut(s) 482
HpyCH4III ACNGT 3 cut(s) 223, 430, 505
HpyCH4IV ACGT 4 cut(s) 142, 227, 310, 1077
HpyCH4V TGCA 4 cut(s) 78, 423, 1048, 1074
HpyF10VI GCNNNNNNNGC 3 cut(s) 564, 805, 1054
HpyF3I CTNAG 7 cut(s) 200, 287, 491, 522, 570, 1229, 1238
HpySE526I ACGT 4 cut(s) 142, 227, 310, 1077
Hsp92I GRCGYC 2 cut(s) 227, 799
Hsp92II CATG 5 cut(s) 673, 1061, 1074, 1206, 1308
HspAI GCGC 2 cut(s) 253, 372
Kzo9I GATC 5 cut(s) 13, 115, 655, 1018, 1133
LmnI GCTCC 2 cut(s) 563, 1108
Lsp1109I GCAGC 2 cut(s) 407, 676
LweI GCATC 1 cut(s) 1057
MaeI CTAG 3 cut(s) 782, 1002, 1175
MaeII ACGT 4 cut(s) 142, 227, 310, 1077
MaeIII GTNAC 2 cut(s) 223, 482
MalI GATC 5 cut(s) 15, 117, 657, 1020, 1135
MboI GATC 5 cut(s) 13, 115, 655, 1018, 1133
MboII GAAGA 5 cut(s) 8, 425, 947, 995, 1277
MhlI GDGCHC 1 cut(s) 381
MluI ACGCGT 1 cut(s) 762
MlyI GAGTC 1 cut(s) 900
MnlI CCTC 3 cut(s) 30, 526, 788
MroXI GAANNNNTTC 1 cut(s) 942
MseI TTAA 6 cut(s) 335, 438, 605, 885, 1199, 1312
MspI CCGG 1 cut(s) 828
MspR9I CCNGG 2 cut(s) 564, 931
MvaI CCWGG 2 cut(s) 564, 931
MvnI CGCG 5 cut(s) 253, 255, 372, 374, 764
MwoI GCNNNNNNNGC 3 cut(s) 564, 805, 1054
NdeI CATATG 1 cut(s) 383
NdeII GATC 5 cut(s) 13, 115, 655, 1018, 1133
NlaIII CATG 5 cut(s) 673, 1061, 1074, 1206, 1308
NlaIV GGNNCC 2 cut(s) 945, 1110
NmuCI GTSAC 2 cut(s) 223, 482
NspI RCATGY 1 cut(s) 1206
NspV TTCGAA 1 cut(s) 475
PciI ACATGT 1 cut(s) 1202
PdmI GAANNNNTTC 1 cut(s) 942
PfeI GAWTC 5 cut(s) 161, 351, 468, 743, 824
PflMI CCANNNNNTGG 2 cut(s) 96, 1028
PkrI GCNGC 5 cut(s) 422, 691, 694, 807, 1113
PleI GAGTC 1 cut(s) 899
PpsI GAGTC 1 cut(s) 899
PpuMI RGGWCCY 1 cut(s) 196
PscI ACATGT 1 cut(s) 1202
PshAI GACNNNNGTC 1 cut(s) 194
PshBI ATTAAT 1 cut(s) 885
Psp1406I AACGTT 1 cut(s) 1077
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 2 cut(s) 562, 929
PspGI CCWGG 2 cut(s) 562, 929
PspN4I GGNNCC 2 cut(s) 945, 1110
PspPI GGNCC 2 cut(s) 196, 628
PspPPI RGGWCCY 1 cut(s) 196
RsaI GTAC 4 cut(s) 141, 449, 455, 1084
RsaNI GTAC 4 cut(s) 140, 448, 454, 1083
SalI GTCGAC 1 cut(s) 48
SaqAI TTAA 6 cut(s) 335, 438, 605, 885, 1199, 1312
SatI GCNGC 5 cut(s) 421, 690, 693, 806, 1112
Sau3AI GATC 5 cut(s) 13, 115, 655, 1018, 1133
Sau96I GGNCC 2 cut(s) 196, 628
SchI GAGTC 1 cut(s) 900
ScrFI CCNGG 2 cut(s) 564, 931
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 1 cut(s) 1057
SfuI TTCGAA 1 cut(s) 475
SinI GGWCC 2 cut(s) 196, 628
SsiI CCGC 5 cut(s) 133, 374, 693, 805, 1112
SspI AATATT 1 cut(s) 883
SspMI CTAG 3 cut(s) 782, 1002, 1175
StyD4I CCNGG 2 cut(s) 562, 929
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 3 cut(s) 223, 430, 505
TaiI ACGT 4 cut(s) 145, 230, 313, 1080
TaqI TCGA 5 cut(s) 49, 230, 349, 475, 793
TatI WGTACW 1 cut(s) 447
TauI GCSGC 3 cut(s) 695, 808, 1114
TfiI GAWTC 5 cut(s) 161, 351, 468, 743, 824
Tru1I TTAA 6 cut(s) 335, 438, 605, 885, 1199, 1312
Tru9I TTAA 6 cut(s) 335, 438, 605, 885, 1199, 1312
TscAI CASTG 1 cut(s) 80
TseFI GTSAC 2 cut(s) 223, 482
TseI GCWGC 2 cut(s) 420, 689
Tsp45I GTSAC 2 cut(s) 223, 482
TspDTI ATGAA 4 cut(s) 686, 718, 802, 1293
TspGWI ACGGA 3 cut(s) 458, 674, 1121
TspRI CASTG 1 cut(s) 80
Van91I CCANNNNNTGG 2 cut(s) 96, 1028
VpaK11BI GGWCC 2 cut(s) 196, 628
VspI ATTAAT 1 cut(s) 885
XapI RAATTY 4 cut(s) 434, 698, 734, 835
XceI RCATGY 1 cut(s) 1206
XmiI GTMKAC 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 942
XspI CTAG 3 cut(s) 782, 1002, 1175
ZraI GACGTC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.