RchiOBHm_Chr7g0182241
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0015627
Physical Location & Seq
Forward (+)
3570158 .. 3571075
918 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16257

Sequence Viewer

Length: 918 bp
ATGCAGTTCATTGAGGTCACTAGGGTTCTTGGAGACATGGGTGTTCCACGGCAGGTGCAGCAAACTATTGTTGCAAAGATATCTGAGTTTCTTGTTCTTGGGAATATGGACCGCACCGCTAATAATGGTTATATTGATGTGACCATAATACACAATGCATCATACATAGATCTAAATATTGTTCCCAGTGATGAGGAAGATGATTCATATAGCTTTGATCAAATGTACATGGATGACTATTTTGATCATGATAGGGTTGCAAGGCAAAGAGGTTTTGATTTAATTGTTGAGCCAGATAATGATGATAGCGAGATCGAATGTGGAGATCCTTTGGATGCAATGTTAGCCAGGTTATATTATGATATTAATCATGTGGATGCGGCGGTTATCAGTATCCATGAGTTGCTTCAAAGGAATCTTCCTACGTTCACAGAAACAAATGATGATCATCATATTAAGGATTTTCAGCTGATGAACTATAGGGATATTGAGGCGGCTGATCAGTATCATAATCGACCACCTCATGAATATCCCTATAATTCGTCCGCTGATCATGATACTGAGGATGAAGATCATTTTGTGCTGGACGAATTGCTAATGGAAACATATGATAATGGTAATGAGGAGGTTGATGGGGCTACATTGGAATCCATAGAAACATATATGGCCATGACCATCCCAGCAAGTAAATCAGCGATAGAGGATTTGAAGAAATTGAGACTCGAGAGTGTGGAAACTGCGAGACATGCTGGGTTATGTAGTATTTGTTTGGAGGACTTTAAAGCCGGAGTTGAAACTACTTGCTTGCCTTGCTTACATTTTTTTCATGGAGATTGCGTTGTTCGATGGCTGGGGATCAATCATCAATGCCCCTTGTGCCGATTTCCAATGCCTCATGACGGAACATCAATCTATTAA

Protein Analysis

305

Amino Acids

34.97

Weight (kDa)

4.36

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 252 - 294 1.8e-13 Ring finger domain
zf-RING_11 PF17123 253 - 280 1.6e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 253 - 293 1.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 253 - 293 2.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AciI CCGC 6 cut(s) 112, 117, 380, 383, 494, 546
AclWI GGATC 2 cut(s) 320, 863
AcoI YGGCCR 1 cut(s) 666
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 899
AgsI TTSAA 3 cut(s) 410, 709, 794
AjnI CCWGG 1 cut(s) 347
AluBI AGCT 2 cut(s) 213, 469
AluI AGCT 2 cut(s) 213, 469
Alw26I GTCTC 3 cut(s) 27, 712, 736
AlwI GGATC 2 cut(s) 320, 863
Ama87I CYCGRG 1 cut(s) 722
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 1 cut(s) 58
AseI ATTAAT 1 cut(s) 366
AspS9I GGNCC 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 722
AvaII GGWCC 1 cut(s) 109
BalI TGGCCA 1 cut(s) 668
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 626, 683, 840
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 1 cut(s) 349
BciVI GTATCC 1 cut(s) 404
BclI TGATCA 5 cut(s) 217, 244, 445, 499, 550
BcoDI GTCTC 3 cut(s) 27, 712, 736
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 43
BfuI GTATCC 1 cut(s) 404
BglII AGATCT 1 cut(s) 169
BisI GCNGC 3 cut(s) 59, 381, 495
BlsI GCNGC 3 cut(s) 60, 382, 496
Bme1390I CCNGG 1 cut(s) 349
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 722
BmgT120I GGNCC 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 349
BmrI ACTGGG 1 cut(s) 180
BmsI GCATC 3 cut(s) 167, 325, 367
BmuI ACTGGG 1 cut(s) 180
BsaBI GATNNNNATC 4 cut(s) 366, 447, 504, 570
BsaJI CCNNGG 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 822, 852
Bsc4I CCNNNNNNNGG 1 cut(s) 899
Bse1I ACTGG 1 cut(s) 186
Bse3DI GCAATG 1 cut(s) 345
Bse8I GATNNNNATC 4 cut(s) 366, 447, 504, 570
BseBI CCWGG 1 cut(s) 349
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 5 cut(s) 238, 340, 382, 571, 675
BseJI GATNNNNATC 4 cut(s) 366, 447, 504, 570
BseLI CCNNNNNNNGG 1 cut(s) 899
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 2 cut(s) 75, 552
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 638
BseXI GCAGC 1 cut(s) 70
BseYI CCCAGC 3 cut(s) 679, 749, 850
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 1 cut(s) 668
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 1 cut(s) 786
BslI CCNNNNNNNGG 1 cut(s) 899
BsmAI GTCTC 3 cut(s) 27, 712, 736
BsnI GGCC 1 cut(s) 668
BsoBI CYCGRG 1 cut(s) 722
Bsp1407I TGTACA 1 cut(s) 225
BspACI CCGC 6 cut(s) 112, 117, 380, 383, 494, 546
BspANI GGCC 1 cut(s) 668
BspCNI CTCAG 2 cut(s) 76, 553
BspHI TCATGA 4 cut(s) 247, 523, 553, 895
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 2 cut(s) 320, 863
BsrDI GCAATG 1 cut(s) 345
BsrGI TGTACA 1 cut(s) 225
BsrI ACTGG 1 cut(s) 186
BssECI CCNNGG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 349
BstAUI TGTACA 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 806
BstDEI CTNAG 2 cut(s) 84, 561
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 5 cut(s) 238, 340, 382, 571, 675
BstMAI GTCTC 3 cut(s) 27, 712, 736
BstMWI GCNNNNNNNGC 5 cut(s) 58, 344, 746, 810, 876
BstNI CCWGG 1 cut(s) 349
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 1 cut(s) 347
BstSFI CTRYAG 1 cut(s) 478
BstV1I GCAGC 1 cut(s) 70
BstX2I RGATCY 2 cut(s) 169, 325
BstYI RGATCY 2 cut(s) 169, 325
BsuI GTATCC 1 cut(s) 404
BsuRI GGCC 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 5 cut(s) 238, 340, 382, 571, 675
BtsIMutI CAGTG 1 cut(s) 193
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 806
CciI TCATGA 4 cut(s) 247, 523, 553, 895
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 226
CviJI RGCY 9 cut(s) 213, 292, 347, 469, 497, 638, 668, 785, 850
CviKI_1 RGCY 9 cut(s) 213, 292, 347, 469, 497, 638, 668, 785, 850
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 2 cut(s) 84, 561
DraI TTTAAA 1 cut(s) 781
EaeI YGGCCR 1 cut(s) 666
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 722
EcoRII CCWGG 1 cut(s) 347
EcoRV GATATC 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 160
FauNDI CATATG 1 cut(s) 607
FbaI TGATCA 5 cut(s) 217, 244, 445, 499, 550
Fnu4HI GCNGC 3 cut(s) 59, 381, 495
FokI GGATG 5 cut(s) 245, 347, 389, 578, 662
Fsp4HI GCNGC 3 cut(s) 59, 381, 495
FspBI CTAG 1 cut(s) 21
GluI GCNGC 3 cut(s) 59, 381, 495
GsaI CCCAGC 3 cut(s) 683, 753, 854
HaeIII GGCC 1 cut(s) 668
HapII CCGG 1 cut(s) 786
HinfI GANTC 4 cut(s) 203, 415, 647, 720
HpaII CCGG 1 cut(s) 786
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 5 cut(s) 248, 524, 554, 724, 896
Hpy8I GTNNAC 1 cut(s) 429
HpyCH4IV ACGT 1 cut(s) 425
HpyCH4V TGCA 6 cut(s) 4, 58, 74, 158, 260, 338
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 344, 746, 810, 876
HpyF3I CTNAG 2 cut(s) 84, 561
HpySE526I ACGT 1 cut(s) 425
Ksp22I TGATCA 5 cut(s) 217, 244, 445, 499, 550
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 3 cut(s) 167, 325, 367
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 2 cut(s) 16, 139
MboII GAAGA 4 cut(s) 209, 410, 581, 721
MflI RGATCY 2 cut(s) 169, 325
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 4 cut(s) 282, 538, 590, 713
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 1 cut(s) 714
Mox20I TGGCCA 1 cut(s) 668
Mph1103I ATGCAT 1 cut(s) 160
MscI TGGCCA 1 cut(s) 668
MseI TTAA 5 cut(s) 281, 366, 456, 780, 916
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 2 cut(s) 469, 548
MspI CCGG 1 cut(s) 786
MspR9I CCNGG 1 cut(s) 349
MvaI CCWGG 1 cut(s) 349
MwoI GCNNNNNNNGC 5 cut(s) 58, 344, 746, 810, 876
NdeI CATATG 1 cut(s) 607
NmuCI GTSAC 2 cut(s) 16, 139
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 1 cut(s) 749
PaeR7I CTCGAG 1 cut(s) 722
PagI TCATGA 4 cut(s) 247, 523, 553, 895
PaqCI CACCTGC 1 cut(s) 43
PfeI GAWTC 3 cut(s) 203, 415, 647
PkrI GCNGC 3 cut(s) 60, 382, 496
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
PshBI ATTAAT 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 347
PspFI CCCAGC 3 cut(s) 679, 749, 850
PspGI CCWGG 1 cut(s) 347
PspPI GGNCC 1 cut(s) 109
PsuI RGATCY 2 cut(s) 169, 325
PvuII CAGCTG 1 cut(s) 469
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 5 cut(s) 281, 366, 456, 780, 916
SatI GCNGC 3 cut(s) 59, 381, 495
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 1 cut(s) 349
SetI ASST 9 cut(s) 18, 57, 215, 274, 353, 428, 471, 523, 630
SfaNI GCATC 3 cut(s) 167, 325, 367
SfcI CTRYAG 1 cut(s) 478
Sfr274I CTCGAG 1 cut(s) 722
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 722
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse9I AATT 4 cut(s) 282, 538, 590, 713
SsiI CCGC 6 cut(s) 112, 117, 380, 383, 494, 546
SspI AATATT 1 cut(s) 178
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 347
TaiI ACGT 1 cut(s) 428
TaqI TCGA 4 cut(s) 315, 514, 723, 844
TasI AATT 4 cut(s) 282, 538, 590, 713
TatI WGTACW 1 cut(s) 225
TauI GCSGC 2 cut(s) 383, 497
TfiI GAWTC 3 cut(s) 203, 415, 647
Tru1I TTAA 5 cut(s) 281, 366, 456, 780, 916
Tru9I TTAA 5 cut(s) 281, 366, 456, 780, 916
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 2 cut(s) 16, 139
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 2 cut(s) 16, 139
TspDTI ATGAA 5 cut(s) 195, 488, 540, 582, 815
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 109
VspI ATTAAT 1 cut(s) 366
XceI RCATGY 1 cut(s) 749
XhoI CTCGAG 1 cut(s) 722
XspI CTAG 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.