Rh7AG049400
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0015627
Physical Location & Seq
Forward (+)
3419421 .. 3420532
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG049400.1

Sequence Viewer

Length: 1035 bp
ATGTCCGGCGGAGTTGCAGTTCCTTCTGTGAGCGACGGCCAGGCTTTATTTAGACACAACCACTACATCGACTATCGCACCCAGGTAGAGTGGGTTCCAATCCCACAGGGAACTACGAGCCTTCCCATCCATATTTATCTCTTCAAGTCAATCGAAACGGAACTGAGCCAAACCATTACCTTCCTTCACAGCAACTATGATATGCAGTTCATTGAGGTCACTAGGGTTCTTGGAGACATGGGTGTTCCACGGCAGGTGCAGCAAACTATTGTTGCAAAGATATCTGAGTTTCTTGTTCTTGGGAATATGGACCGCACCGCTAATAATGGTTATATTGATGTGACCATAATACACGATGCATCATACATAGATCTAAATATTGTTCCCAGTGATGAGGAAGATGATTCATATAGCTTTGATCAAATGTACATGGATGACTATTTTGATCATGATAGGATTGCAAGGCAAAGAGGTTTTGATTTAATCGTTGAGCCAGATAATGATGATAGCGAGATCGAATGTGGAGATCCTTTGGATGCAATGTTAGCCAGGTTATATTATGATATTAATCATGTGGATGCGGCGGTTATCGACTTATCGCTGATGAACTATAGGGATATTGAGGCGGCTGATCAGTATCATAATCGACCACCTCATGAATATCCCTATAATTCGTCCGCTGATCATGATACTGAGGATGAAGATCATTTTGTGCTGGACGAATTGCTAATGGAAACATATGATAATGGTAATGAGGAGATTGATGGGGCTACATTGGAATCCATAGAAACATATATGGCCATGACCATCCCAGCAAGTAAATCAGCGATAGAGGATTTGAAGAAATTGAGACTCGAGAGTGTGGAAACTGCGAGACATGCTGGGTTATGTAGTATTTGTTTGGAGGACTTTAAAGCCGGAGTTGAAGCTACTAGATTGCCTTGCTTACATTTTTTTCATGGAGATTGCGTTGTTCGGTGGCTGGAGATCAATCATCAATGCCCCTTGTGCCGATTTCCAATGCCTCATGACTGA

Protein Analysis

344

Amino Acids

39.34

Weight (kDa)

4.39

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 296 - 338 1.7e-14 Ring finger domain
zf-RING_11 PF17123 297 - 324 3.1e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 297 - 337 2.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 297 - 337 1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 297 - 338 8.5e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 244
AciI CCGC 7 cut(s) 9, 313, 318, 581, 584, 626, 678
AclWI GGATC 1 cut(s) 521
AcoI YGGCCR 2 cut(s) 37, 798
AfaI GTAC 1 cut(s) 428
AgsI TTSAA 3 cut(s) 145, 841, 926
AjnI CCWGG 3 cut(s) 39, 81, 548
AloI GAACNNNNNNTCC 1 cut(s) 35
AluBI AGCT 2 cut(s) 414, 929
AluI AGCT 2 cut(s) 414, 929
Alw26I GTCTC 3 cut(s) 228, 844, 868
AlwI GGATC 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 2 cut(s) 37, 798
ApeKI GCWGC 1 cut(s) 259
AseI ATTAAT 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 1 cut(s) 310
BalI TGGCCA 1 cut(s) 800
BbvI GCAGC 1 cut(s) 271
BccI CCATC 3 cut(s) 134, 758, 815
BceAI ACGGC 2 cut(s) 52, 266
BciT130I CCWGG 3 cut(s) 41, 83, 550
BclI TGATCA 4 cut(s) 418, 445, 631, 682
BcoDI GTCTC 3 cut(s) 228, 844, 868
BfaI CTAG 2 cut(s) 222, 933
BfmI CTRYAG 1 cut(s) 610
BfuAI ACCTGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 370
BisI GCNGC 3 cut(s) 260, 582, 627
BlsI GCNGC 3 cut(s) 261, 583, 628
Bme1390I CCNGG 3 cut(s) 41, 83, 550
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 3 cut(s) 41, 83, 550
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 4 cut(s) 346, 368, 526, 568
BmuI ACTGGG 1 cut(s) 381
BpmI CTGGAG 1 cut(s) 1004
BsaBI GATNNNNATC 3 cut(s) 567, 636, 702
BsaJI CCNNGG 2 cut(s) 81, 248
BsaXI ACNNNNNCTCC 3 cut(s) 33, 954, 984
Bse1I ACTGG 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 3 cut(s) 567, 636, 702
BseBI CCWGG 3 cut(s) 41, 83, 550
BseDI CCNNGG 2 cut(s) 81, 248
BseGI GGATG 6 cut(s) 126, 439, 541, 583, 703, 807
BseJI GATNNNNATC 3 cut(s) 567, 636, 702
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 3 cut(s) 155, 276, 684
BseNI ACTGG 1 cut(s) 387
BseRI GAGGAG 1 cut(s) 770
BseXI GCAGC 1 cut(s) 271
BseYI CCCAGC 2 cut(s) 811, 881
BsgI GTGCAG 1 cut(s) 278
BshFI GGCC 2 cut(s) 39, 800
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 2 cut(s) 6, 918
BsmAI GTCTC 3 cut(s) 228, 844, 868
BsnI GGCC 2 cut(s) 39, 800
BsoBI CYCGRG 1 cut(s) 854
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
BspACI CCGC 7 cut(s) 9, 313, 318, 581, 584, 626, 678
BspANI GGCC 2 cut(s) 39, 800
BspCNI CTCAG 3 cut(s) 156, 277, 685
BspHI TCATGA 4 cut(s) 448, 655, 685, 1027
BspLI GGNNCC 1 cut(s) 96
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 546
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 1 cut(s) 387
BssECI CCNNGG 2 cut(s) 81, 248
BssMI GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
Bst2UI CCWGG 3 cut(s) 41, 83, 550
Bst6I CTCTTC 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 426
BstDEI CTNAG 3 cut(s) 164, 285, 693
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 6 cut(s) 126, 439, 541, 583, 703, 807
BstKTI GATC 9 cut(s) 373, 421, 448, 516, 529, 634, 685, 706, 990
BstMAI GTCTC 3 cut(s) 228, 844, 868
BstMBI GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
BstMWI GCNNNNNNNGC 4 cut(s) 259, 545, 878, 1008
BstNI CCWGG 3 cut(s) 41, 83, 550
BstNSI RCATGY 1 cut(s) 881
BstSCI CCNGG 3 cut(s) 39, 81, 548
BstSFI CTRYAG 1 cut(s) 610
BstV1I GCAGC 1 cut(s) 271
BstX2I RGATCY 2 cut(s) 370, 526
BstYI RGATCY 2 cut(s) 370, 526
BsuRI GGCC 2 cut(s) 39, 800
BtgI CCRYGG 1 cut(s) 248
BtsCI GGATG 6 cut(s) 126, 439, 541, 583, 703, 807
BtsIMutI CAGTG 1 cut(s) 394
BveI ACCTGC 1 cut(s) 244
CciI TCATGA 4 cut(s) 448, 655, 685, 1027
Cfr13I GGNCC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 427
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 3 cut(s) 164, 285, 693
DpnI GATC 9 cut(s) 372, 420, 447, 515, 528, 633, 684, 705, 989
DpnII GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
DraI TTTAAA 1 cut(s) 913
EaeI YGGCCR 2 cut(s) 37, 798
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
EciI GGCGGA 1 cut(s) 24
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 854
EcoRII CCWGG 3 cut(s) 39, 81, 548
EcoRV GATATC 1 cut(s) 282
EcoT22I ATGCAT 1 cut(s) 361
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 4 cut(s) 418, 445, 631, 682
Fnu4HI GCNGC 3 cut(s) 260, 582, 627
FokI GGATG 6 cut(s) 113, 446, 548, 590, 710, 794
Fsp4HI GCNGC 3 cut(s) 260, 582, 627
FspBI CTAG 2 cut(s) 222, 933
GluI GCNGC 3 cut(s) 260, 582, 627
GsaI CCCAGC 2 cut(s) 815, 885
GsuI CTGGAG 1 cut(s) 1004
HaeIII GGCC 2 cut(s) 39, 800
HapII CCGG 2 cut(s) 6, 918
HinfI GANTC 3 cut(s) 404, 779, 852
HpaII CCGG 2 cut(s) 6, 918
Hpy188I TCNGA 1 cut(s) 286
Hpy188III TCNNGA 5 cut(s) 449, 656, 686, 856, 1028
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 4 cut(s) 33, 131, 190, 194
HpyCH4V TGCA 7 cut(s) 17, 205, 259, 275, 359, 461, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 259, 545, 878, 1008
HpyF3I CTNAG 3 cut(s) 164, 285, 693
Ksp22I TGATCA 4 cut(s) 418, 445, 631, 682
Kzo9I GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
Lsp1109I GCAGC 1 cut(s) 271
LweI GCATC 4 cut(s) 346, 368, 526, 568
MaeI CTAG 2 cut(s) 222, 933
MaeIII GTNAC 2 cut(s) 217, 340
MalI GATC 9 cut(s) 372, 420, 447, 515, 528, 633, 684, 705, 989
MboI GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
MboII GAAGA 4 cut(s) 133, 410, 713, 853
MflI RGATCY 2 cut(s) 370, 526
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 3 cut(s) 670, 722, 845
MluNI TGGCCA 1 cut(s) 800
MlyI GAGTC 1 cut(s) 846
Mox20I TGGCCA 1 cut(s) 800
Mph1103I ATGCAT 1 cut(s) 361
MscI TGGCCA 1 cut(s) 800
MseI TTAA 3 cut(s) 482, 567, 912
MslI CAYNNNNRTG 1 cut(s) 576
Msp20I TGGCCA 1 cut(s) 800
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 2 cut(s) 6, 918
MspR9I CCNGG 3 cut(s) 41, 83, 550
MvaI CCWGG 3 cut(s) 41, 83, 550
MwoI GCNNNNNNNGC 4 cut(s) 259, 545, 878, 1008
NdeI CATATG 1 cut(s) 739
NdeII GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 217, 340
NsiI ATGCAT 1 cut(s) 361
NspI RCATGY 1 cut(s) 881
PaeR7I CTCGAG 1 cut(s) 854
PagI TCATGA 4 cut(s) 448, 655, 685, 1027
PaqCI CACCTGC 1 cut(s) 244
PfeI GAWTC 2 cut(s) 404, 779
PkrI GCNGC 3 cut(s) 261, 583, 628
PleI GAGTC 1 cut(s) 846
PpsI GAGTC 1 cut(s) 846
PshBI ATTAAT 1 cut(s) 567
Psp6I CCWGG 3 cut(s) 39, 81, 548
PspFI CCCAGC 2 cut(s) 811, 881
PspGI CCWGG 3 cut(s) 39, 81, 548
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 1 cut(s) 310
PsrI GAACNNNNNNTAC 2 cut(s) 78, 110
PsuI RGATCY 2 cut(s) 370, 526
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
RseI CAYNNNNRTG 1 cut(s) 576
SaqAI TTAA 3 cut(s) 482, 567, 912
SatI GCNGC 3 cut(s) 260, 582, 627
Sau3AI GATC 9 cut(s) 370, 418, 445, 513, 526, 631, 682, 703, 987
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 846
ScrFI CCNGG 3 cut(s) 41, 83, 550
SetI ASST 9 cut(s) 87, 182, 219, 258, 416, 475, 554, 655, 931
SfaNI GCATC 4 cut(s) 346, 368, 526, 568
SfcI CTRYAG 1 cut(s) 610
Sfr274I CTCGAG 1 cut(s) 854
SinI GGWCC 1 cut(s) 310
SlaI CTCGAG 1 cut(s) 854
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 3 cut(s) 670, 722, 845
SsiI CCGC 7 cut(s) 9, 313, 318, 581, 584, 626, 678
SspI AATATT 1 cut(s) 379
SspMI CTAG 2 cut(s) 222, 933
StyD4I CCNGG 3 cut(s) 39, 81, 548
TaqI TCGA 6 cut(s) 69, 153, 516, 591, 646, 855
TasI AATT 3 cut(s) 670, 722, 845
TatI WGTACW 1 cut(s) 426
TauI GCSGC 2 cut(s) 584, 629
TfiI GAWTC 2 cut(s) 404, 779
Tru1I TTAA 3 cut(s) 482, 567, 912
Tru9I TTAA 3 cut(s) 482, 567, 912
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 2 cut(s) 217, 340
TseI GCWGC 1 cut(s) 259
Tsp45I GTSAC 2 cut(s) 217, 340
TspDTI ATGAA 6 cut(s) 199, 396, 620, 672, 714, 947
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 310
VspI ATTAAT 1 cut(s) 567
XceI RCATGY 1 cut(s) 881
XhoI CTCGAG 1 cut(s) 854
XspI CTAG 2 cut(s) 222, 933
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.