Rorug06G0446400
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004694
Physical Location & Seq
Forward (+)
57730464 .. 57737426
6963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0446400.1

Sequence Viewer

Length: 2865 bp
ATGAGCGGCCCCAAGGCCATCAGCCTCGAGGAGATCAAAAACGAGTCCGTCGATCTGGAACGGATTCCAATTGAGGAAGTCTTTGAGCAGTTGAAATGTACAAGGGAAGGTCTTACCTCCGACGAGGGTCAGAACAGGCTCCAAGTCTTTGGACCAAACAAGTTAGAGGAGAAAAAGGAAAACAAACTACTCAAGTTTTTGGGGTTCATGTGGAATCCCTTGTCATGGGTCATGGAAGCTGCTGCTTTGATGGCTATTGTCCTGGCAAATGGTGATGGAAGGCCTCCAGATTGGCAGGATTTTGTCGGAATTATGGTCCTGCTGGTCGTTAACTCTACCATCAGTTTTATTGAGGAAAACAATGCTGGTAATGCGGCTGCTGCACTTATGGCTGGTCTTGCTCCTAAGACTAAGGTCCTCAGAGATGGTCGATGGACAGAGCAAGATGCTTCAATTTTGGTTCCAGGAGACATAATCAGCATTAAGTTGGGAGACATAGTGCCTGCTGATGCTCGCCTTCTTGAGGGTGATCCTCTGAAGATTGACCAGTCTGCCCTAACCGGCGAGTCCCTTGCTGTTACTAAAAATCCTGGAGATGAAGTGTTTTCTGGCTCAACATGTAAACAGGGTGAAATTGAAGCAGTGGTCATTGCGACTGGTGTGCACACCTTCTTTGGTAAAGCTGCACATCTTGTGGACAGCACCAACAATGTTGGGCATTTCCAGCAAGTTCTCACAGCCATTGGGAACTTCTGCATTTGTTCAATTGCTGTGGGAATACTTATTGAGATTGTTGTCATGTACCCAATTCAGAGGCGCAAGTACAGGGATGGGATTGATAATTTGCTGGTTCTCTTGATTGGTGGAATCCCAATTGCTATGCCGACTGTTTTATCTGTCACGATGGCTATAGGTTCTCACAGGCTTTCTCAACAAGGTGCTATTACTAAGAGAATGACTGCCATTGAGGAAATGGCTGGCATGGATGTACTGTGCAGTGATAAGACTGGGACCTTGACCCTGAACAAGCTGACAGTTGATAGAAATCTGATTGAAGTGTTTCCCAAGGGTGTGGAGAAAGAGCATGTTATCCTTCTTGCAGCAAGGGCTTCTAGGACTGAAAATCAGGATGCTATTGATGCTGCCATAGTAAATATGCTTGCTGATCCAAAGGAGGCGCGAGCTGGTGTCAGAGAAATCCATTTCCTTCCATTCAACCCTGTTGACAAGAGGACTGCTTTGACCTACATTGATTCTGATGGGAATTGGCATCGATCTAGCAAAGGTGCCCCTGAGCAGATATTAGACCTATGCAACTGCAAGGAAGATTTCAAGAAAAAGGTTCATGCCGTGATTGATAAATTTGCCGAACGTGGGCTTCGATCTCTTGGGGTTGCAAGACAGCAAATCCCTGAGAAAACAAAAGAGAGTCCAGGAGCACCATGGCAGTTTGTTGGTTTGTTGCCGTTATTTGATCCTCCCAGGCATGACAGTGCAGAAACTATTCGCAGAGCTCTCAATCTTGGTGTGAATGTGAAGATGATCACTGGGGATCAGCTGGCCATTGGCAAGGAAACTGGACGGCGGCTTGGTATGGGAACAAACATGTATCCATCTTCTGCATTGCTCGGCCAAGACAAGGATGCGTCCATTTCTGCTCTCCCCATTGATGAATTGATTGAGAAGGCTGATGGATTTGCTGGAGTATTTCCAGAGCACAAATATGAAATTGTAAGGAGGCTGCAAGAGAGGAAGCACATCTGTGGAATGACTGGTGATGGTGTCAACGATGCTCCTGCTTTGAAAAAAGCAGACATTGGAATTGCTGTAGCTGATGCTACTGATGCTGCTAGAGGTGCTTCAGATATTGTTCTGACTGAACCTGGATTAAGTGTGATAATAAGCGCAGTACTTACAAGCAGGGCCATATTCCAGAGGATGAAGAACTATACTATCTACGCCGTTTCAATTACCATACGTATAGTGTTTGGCTTTATGTTCATCGCTTTGATATGGAAGTTTGACTTTGCACCCTTCATGGTATTGATCATCGCTATACTAAATGATGGAACGATAATGACAATATCCAAGGACCGAGTGAAACCATCTCCAATGCCAGACAGCTGGAAACTGAAAGAAATTTTTGCTACTGGCATTGTTCTTGGAGGTTACATGGCCTTGATGACAGTAGTGTTTTTTTGGTTGATGCGAGATACAGACTTCTTCTCGAAGACGTTTGGTGTGCGATCTTTGAGGTATAGCCCTAGTGAAATGATGGCAGCGTTATACTTACAAGTGAGTATTATTAGCCAAGCCCTTATCTTTGTCACAAGGTCCCGCAGCTTTTCCTTTATGGAACGCCCTGGACTTCTCTTGCTTGGAGCTTTCATCGTTGCTCAATTGGTGGCGACTCTGATAGCGGTCTATGCCAGCTGGGGTTTTGCACGGATAAAGGGATGTGGCTGGGGATGGGCTGGTGTTATCTGGATTTTCAGTGTGGTGACATATATACCTCTTGATTTTCTGAAGTTTGCAATCCGCTACATCCTTAGTGGAAAAGCTTGGGATAACCTTTTGGAGAACAAGACTGCGTTTACTACAAAGAAAGACTATGGAAAAGAGGAGAGAGAAGCACAGTGGGCTACCGCACAAAGGACCCTACATGGTCTTCAACCACCTGAAAGCAGCAATATCTTCAGTGACAAGAGCAGTTACCGGGAACTGTCAGAGATTGCAGAACAAGCTAAGAGAAGGGCTGAGGTTGCAAGGCTTAGGGAGTTGACTACATTGAAGGGTCATGTTGAGTCTGTTGTGAAGCTGAAAGGGCTCGACATTGATAATATCCAACAGCATTATACAGTTTGA

Protein Analysis

954

Amino Acids

105.18

Weight (kDa)

6.74

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 21 - 84 1.2e-14 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 134 - 232 2.4e-30 P-type ATPase actuator domain
Hydrolase PF00702 328 - 605 1.9e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 375 - 496 1.7e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 1286
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 1 cut(s) 1180
AciI CCGC 7 cut(s) 6, 374, 1585, 2338, 2420, 2539, 2646
AclWI GGATC 4 cut(s) 524, 1160, 1469, 1560
AcoI YGGCCR 2 cut(s) 1560, 1630
AcsI RAATTY 2 cut(s) 1361, 2139
AcuI CTGAAG 4 cut(s) 557, 1845, 2546, 2680
AfaI GTAC 5 cut(s) 100, 803, 824, 990, 1911
AfiI CCNNNNNNNGG 5 cut(s) 225, 523, 1374, 1639, 2652
AflIII ACRYGT 2 cut(s) 617, 1605
AjnI CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
AleI CACNNNNGTG 1 cut(s) 1761
Alw21I GWGCWC 4 cut(s) 666, 1441, 1516, 1719
Alw26I GTCTC 2 cut(s) 462, 486
Alw44I GTGCAC 1 cut(s) 662
AlwI GGATC 4 cut(s) 524, 1160, 1469, 1560
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 7 cut(s) 7, 15, 281, 1560, 1630, 1923, 2175
ApaLI GTGCAC 1 cut(s) 662
ApoI RAATTY 2 cut(s) 1361, 2139
Asp700I GAANNNNTTC 3 cut(s) 63, 1059, 1503
AspLEI GCGC 3 cut(s) 819, 1180, 1907
AspS9I GGNCC 9 cut(s) 8, 152, 316, 415, 1011, 1923, 2092, 2334, 2655
AsuC2I CCSGG 1 cut(s) 2717
AsuHPI GGTGA 5 cut(s) 284, 539, 641, 1787, 2512
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 7 cut(s) 152, 316, 415, 1011, 2092, 2334, 2655
BaeGI GKGCMC 2 cut(s) 666, 1291
BalI TGGCCA 1 cut(s) 1562
BanI GGYRCC 1 cut(s) 1286
BanII GRGCYC 2 cut(s) 1516, 2829
BbsI GAAGAC 2 cut(s) 2237, 2660
Bbv12I GWGCWC 4 cut(s) 666, 1441, 1516, 1719
BbvCI CCTCAGC 1 cut(s) 2757
BceAI ACGGC 4 cut(s) 1334, 1450, 1598, 1946
BcgI CGANNNNNNTGC 6 cut(s) 554, 588, 643, 677, 1778, 1812
BciT130I CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
BciVI GTATCC 1 cut(s) 1620
BclI TGATCA 2 cut(s) 1542, 2046
BcnI CCSGG 1 cut(s) 2717
BcoDI GTCTC 2 cut(s) 462, 486
BfaI CTAG 4 cut(s) 1113, 1278, 1851, 2265
BfmI CTRYAG 2 cut(s) 909, 1827
BfuI GTATCC 1 cut(s) 1620
BmcAI AGTACT 1 cut(s) 1911
Bme1390I CCNGG 8 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364, 2717
Bme18I GGWCC 7 cut(s) 152, 316, 415, 1011, 2092, 2334, 2655
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 9 cut(s) 8, 152, 316, 415, 1011, 1923, 2092, 2334, 2655
BmiI GGNNCC 7 cut(s) 10, 140, 462, 1012, 1288, 2336, 2657
BmrFI CCNGG 8 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364, 2717
BmrI ACTGGG 2 cut(s) 1017, 1557
BmuI ACTGGG 2 cut(s) 1017, 1557
BoxI GACNNNNGTC 2 cut(s) 126, 413
BpiI GAAGAC 2 cut(s) 2237, 2660
BpmI CTGGAG 3 cut(s) 270, 612, 1722
Bpu10I CCTNAGC 3 cut(s) 1293, 2757, 2771
BpuEI CTTGAG 2 cut(s) 176, 542
BpuMI CCSGG 1 cut(s) 2717
Bsa29I ATCGAT 1 cut(s) 1273
BsaAI YACGTR 1 cut(s) 1979
BsaBI GATNNNNATC 1 cut(s) 1044
BsaJI CCNNGG 6 cut(s) 12, 1065, 1442, 1481, 2088, 2362
BsaXI ACNNNNNCTCC 4 cut(s) 483, 513, 585, 615
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 523, 1374, 1639, 2652
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 7 cut(s) 547, 661, 1012, 1552, 1582, 1777, 2155
Bse3DI GCAATG 2 cut(s) 648, 1622
Bse8I GATNNNNATC 1 cut(s) 1044
BseBI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
BseCI ATCGAT 1 cut(s) 1273
BseDI CCNNGG 6 cut(s) 12, 1065, 1442, 1481, 2088, 2362
BseGI GGATG 8 cut(s) 835, 991, 1135, 1648, 1944, 2462, 2474, 2544
BseJI GATNNNNATC 1 cut(s) 1044
BseLI CCNNNNNNNGG 5 cut(s) 225, 523, 1374, 1639, 2652
BseMI GCAATG 2 cut(s) 648, 1622
BseMII CTCAG 4 cut(s) 433, 1284, 1404, 2748
BseNI ACTGG 7 cut(s) 547, 661, 1012, 1552, 1582, 1777, 2155
BseRI GAGGAG 3 cut(s) 44, 182, 2636
BseSI GKGCMC 2 cut(s) 666, 1291
BseYI CCCAGC 2 cut(s) 2433, 2463
BsgI GTGCAG 4 cut(s) 366, 669, 1015, 1515
Bsh1236I CGCG 1 cut(s) 1180
BshFI GGCC 7 cut(s) 9, 17, 283, 1562, 1632, 1925, 2177
BshNI GGYRCC 1 cut(s) 1286
BshVI ATCGAT 1 cut(s) 1273
BsiHKAI GWGCWC 4 cut(s) 666, 1441, 1516, 1719
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 561, 2716
BslFI GGGAC 3 cut(s) 553, 1024, 2320
BslI CCNNNNNNNGG 5 cut(s) 225, 523, 1374, 1639, 2652
BsmAI GTCTC 2 cut(s) 462, 486
BsmFI GGGAC 3 cut(s) 553, 1024, 2320
BsnI GGCC 7 cut(s) 9, 17, 283, 1562, 1632, 1925, 2177
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 6 cut(s) 666, 1291, 1441, 1516, 1719, 2829
Bsp1407I TGTACA 1 cut(s) 98
Bsp19I CCATGG 1 cut(s) 1442
BspACI CCGC 7 cut(s) 6, 374, 1585, 2338, 2420, 2539, 2646
BspANI GGCC 7 cut(s) 9, 17, 283, 1562, 1632, 1925, 2177
BspCNI CTCAG 4 cut(s) 432, 1285, 1405, 2749
BspDI ATCGAT 1 cut(s) 1273
BspFNI CGCG 1 cut(s) 1180
BspLI GGNNCC 7 cut(s) 10, 140, 462, 1012, 1288, 2336, 2657
BspPI GGATC 4 cut(s) 524, 1160, 1469, 1560
BspT107I GGYRCC 1 cut(s) 1286
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 648, 1622
BsrFI RCCGGY 1 cut(s) 560
BsrGI TGTACA 1 cut(s) 98
BsrI ACTGG 7 cut(s) 547, 661, 1012, 1552, 1582, 1777, 2155
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 6 cut(s) 12, 1065, 1442, 1481, 2088, 2362
BssT1I CCWWGG 4 cut(s) 12, 1065, 1442, 2088
Bst2UI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
Bst4CI ACNGT 8 cut(s) 889, 993, 1036, 1493, 2188, 2637, 2724, 2860
BstAUI TGTACA 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 1979
BstC8I GCNNGC 7 cut(s) 504, 514, 979, 1161, 1182, 1560, 2431
BstDSI CCRYGG 1 cut(s) 1442
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 8 cut(s) 835, 991, 1135, 1648, 1944, 2462, 2474, 2544
BstFNI CGCG 1 cut(s) 1180
BstHHI GCGC 3 cut(s) 819, 1180, 1907
BstMAI GTCTC 2 cut(s) 462, 486
BstNI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
BstNSI RCATGY 3 cut(s) 621, 1088, 1609
BstPAI GACNNNNGTC 2 cut(s) 126, 413
BstSCI CCNGG 8 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362, 2715
BstSFI CTRYAG 2 cut(s) 909, 1827
BstSLI GKGCMC 2 cut(s) 666, 1291
BstSNI TACGTA 1 cut(s) 1979
BstUI CGCG 1 cut(s) 1180
BstV2I GAAGAC 2 cut(s) 2237, 2660
BstXI CCANNNNNNTGG 3 cut(s) 149, 1072, 2124
Bsu15I ATCGAT 1 cut(s) 1273
BsuI GTATCC 1 cut(s) 1620
BsuRI GGCC 7 cut(s) 9, 17, 283, 1562, 1632, 1925, 2177
BsuTUI ATCGAT 1 cut(s) 1273
BtgI CCRYGG 1 cut(s) 1442
BtgZI GCGATG 2 cut(s) 1987, 2035
BtsCI GGATG 8 cut(s) 835, 991, 1135, 1648, 1944, 2462, 2474, 2544
BtsI GCAGTG 2 cut(s) 648, 1003
BtsIMutI CAGTG 7 cut(s) 648, 1003, 1498, 1545, 2500, 2642, 2704
Cac8I GCNNGC 7 cut(s) 504, 514, 979, 1161, 1182, 1560, 2431
CfoI GCGC 3 cut(s) 819, 1180, 1907
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 9 cut(s) 8, 152, 316, 415, 1011, 1923, 2092, 2334, 2655
ClaI ATCGAT 1 cut(s) 1273
CseI GACGC 1 cut(s) 1635
Csp6I GTAC 5 cut(s) 99, 802, 823, 989, 1910
CspCI CAANNNNNGTGG 2 cut(s) 753, 788
CviQI GTAC 5 cut(s) 99, 802, 823, 989, 1910
EaeI YGGCCR 2 cut(s) 1560, 1630
Ecl136II GAGCTC 1 cut(s) 1514
Eco105I TACGTA 1 cut(s) 1979
Eco130I CCWWGG 4 cut(s) 12, 1065, 1442, 2088
Eco147I AGGCCT 1 cut(s) 283
Eco24I GRGCYC 2 cut(s) 1516, 2829
Eco47I GGWCC 7 cut(s) 152, 316, 415, 1011, 2092, 2334, 2655
Eco53kI GAGCTC 1 cut(s) 1514
Eco57I CTGAAG 4 cut(s) 557, 1845, 2546, 2680
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 1514
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoO109I RGGNCCY 4 cut(s) 415, 1011, 2334, 2655
EcoRII CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
EcoT14I CCWWGG 4 cut(s) 12, 1065, 1442, 2088
EcoT38I GRGCYC 2 cut(s) 1516, 2829
ErhI CCWWGG 4 cut(s) 12, 1065, 1442, 2088
FalI AAGNNNNNCTT 2 cut(s) 2009, 2041
FaqI GGGAC 3 cut(s) 553, 1024, 2320
FauI CCCGC 1 cut(s) 2345
FbaI TGATCA 2 cut(s) 1542, 2046
FokI GGATG 8 cut(s) 842, 998, 1142, 1655, 1951, 2469, 2481, 2531
FriOI GRGCYC 2 cut(s) 1516, 2829
FspBI CTAG 4 cut(s) 1113, 1278, 1851, 2265
GlaI GCGC 3 cut(s) 818, 1179, 1906
GsaI CCCAGC 2 cut(s) 2437, 2467
GsuI CTGGAG 3 cut(s) 270, 612, 1722
HaeIII GGCC 7 cut(s) 9, 17, 283, 1562, 1632, 1925, 2177
HapII CCGG 2 cut(s) 561, 2716
HgaI GACGC 1 cut(s) 1635
HhaI GCGC 3 cut(s) 819, 1180, 1907
Hin6I GCGC 3 cut(s) 817, 1178, 1905
HinP1I GCGC 3 cut(s) 817, 1178, 1905
HincII GTYRAC 4 cut(s) 331, 1225, 1786, 2781
HindII GTYRAC 4 cut(s) 331, 1225, 1786, 2781
HindIII AAGCTT 1 cut(s) 2559
HinfI GANTC 9 cut(s) 44, 64, 214, 566, 867, 1253, 1429, 2410, 2804
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 2 cut(s) 561, 2716
HphI GGTGA 5 cut(s) 284, 539, 641, 1787, 2512
Hpy166II GTNNAC 8 cut(s) 331, 623, 664, 697, 1225, 1786, 2595, 2781
Hpy8I GTNNAC 8 cut(s) 331, 623, 664, 697, 1225, 1786, 2595, 2781
Hpy99I CGWCG 2 cut(s) 53, 125
HpyCH4III ACNGT 8 cut(s) 889, 993, 1036, 1493, 2188, 2637, 2724, 2860
HpyCH4IV ACGT 3 cut(s) 1372, 1978, 2234
HpySE526I ACGT 3 cut(s) 1372, 1978, 2234
HspAI GCGC 3 cut(s) 817, 1178, 1905
Ksp22I TGATCA 2 cut(s) 1542, 2046
KspAI GTTAAC 1 cut(s) 331
LmnI GCTCC 5 cut(s) 144, 406, 1436, 1798, 2381
MaeI CTAG 4 cut(s) 1113, 1278, 1851, 2265
MaeII ACGT 3 cut(s) 1372, 1978, 2234
MaeIII GTNAC 7 cut(s) 577, 898, 2168, 2326, 2500, 2699, 2711
MbiI CCGCTC 1 cut(s) 6
MboII GAAGA 9 cut(s) 550, 1337, 1549, 1608, 1954, 2215, 2242, 2660, 2686
MfeI CAATTG 4 cut(s) 69, 765, 873, 2399
MhlI GDGCHC 6 cut(s) 666, 1291, 1441, 1516, 1719, 2829
MlsI TGGCCA 1 cut(s) 1562
MluNI TGGCCA 1 cut(s) 1562
MlyI GAGTC 5 cut(s) 53, 575, 1438, 2404, 2813
MmeI TCCRAC 2 cut(s) 144, 286
Mox20I TGGCCA 1 cut(s) 1562
MroXI GAANNNNTTC 3 cut(s) 63, 1059, 1503
MscI TGGCCA 1 cut(s) 1562
MseI TTAA 3 cut(s) 330, 483, 1889
MslI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
Msp20I TGGCCA 1 cut(s) 1562
MspA1I CMGCKG 3 cut(s) 1558, 2124, 2433
MspI CCGG 2 cut(s) 561, 2716
MspR9I CCNGG 8 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364, 2717
MunI CAATTG 4 cut(s) 69, 765, 873, 2399
MvaI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
MvnI CGCG 1 cut(s) 1180
NciI CCSGG 1 cut(s) 2717
NcoI CCATGG 1 cut(s) 1442
NlaIV GGNNCC 7 cut(s) 10, 140, 462, 1012, 1288, 2336, 2657
NmeAIII GCCGAG 1 cut(s) 1608
NmuCI GTSAC 4 cut(s) 898, 2326, 2500, 2699
NspI RCATGY 3 cut(s) 621, 1088, 1609
OliI CACNNNNGTG 1 cut(s) 1761
PaeR7I CTCGAG 1 cut(s) 26
PceI AGGCCT 1 cut(s) 283
PciI ACATGT 2 cut(s) 617, 1605
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 1378
PdmI GAANNNNTTC 3 cut(s) 63, 1059, 1503
PfeI GAWTC 4 cut(s) 64, 214, 867, 1253
PfoI TCCNGGA 3 cut(s) 463, 589, 1432
PleI GAGTC 5 cut(s) 52, 574, 1437, 2404, 2812
PpsI GAGTC 5 cut(s) 52, 574, 1437, 2404, 2812
Ppu21I YACGTR 1 cut(s) 1979
PpuMI RGGWCCY 4 cut(s) 415, 1011, 2334, 2655
PscI ACATGT 2 cut(s) 617, 1605
PshAI GACNNNNGTC 2 cut(s) 126, 413
Psp124BI GAGCTC 1 cut(s) 1516
Psp5II RGGWCCY 4 cut(s) 415, 1011, 2334, 2655
Psp6I CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
PspFI CCCAGC 2 cut(s) 2433, 2463
PspGI CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
PspN4I GGNNCC 7 cut(s) 10, 140, 462, 1012, 1288, 2336, 2657
PspPI GGNCC 9 cut(s) 8, 152, 316, 415, 1011, 1923, 2092, 2334, 2655
PspPPI RGGWCCY 4 cut(s) 415, 1011, 2334, 2655
PspXI VCTCGAGB 1 cut(s) 26
PvuII CAGCTG 3 cut(s) 1558, 2124, 2433
RsaI GTAC 5 cut(s) 100, 803, 824, 990, 1911
RsaNI GTAC 5 cut(s) 99, 802, 823, 989, 1910
RseI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
SacI GAGCTC 1 cut(s) 1516
SaqAI TTAA 3 cut(s) 330, 483, 1889
Sau96I GGNCC 9 cut(s) 8, 152, 316, 415, 1011, 1923, 2092, 2334, 2655
ScaI AGTACT 1 cut(s) 1911
SchI GAGTC 5 cut(s) 53, 575, 1438, 2404, 2813
ScrFI CCNGG 8 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364, 2717
SduI GDGCHC 6 cut(s) 666, 1291, 1441, 1516, 1719, 2829
SfcI CTRYAG 2 cut(s) 909, 1827
Sfr274I CTCGAG 1 cut(s) 26
SinI GGWCC 7 cut(s) 152, 316, 415, 1011, 2092, 2334, 2655
SlaI CTCGAG 1 cut(s) 26
SmiMI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
SmlI CTYRAG 3 cut(s) 26, 191, 521
SmoI CTYRAG 3 cut(s) 26, 191, 521
SnaBI TACGTA 1 cut(s) 1979
SseBI AGGCCT 1 cut(s) 283
SsiI CCGC 7 cut(s) 6, 374, 1585, 2338, 2420, 2539, 2646
SspMI CTAG 4 cut(s) 1113, 1278, 1851, 2265
SstI GAGCTC 1 cut(s) 1516
StuI AGGCCT 1 cut(s) 283
StyD4I CCNGG 8 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362, 2715
StyI CCWWGG 4 cut(s) 12, 1065, 1442, 2088
TaaI ACNGT 8 cut(s) 889, 993, 1036, 1493, 2188, 2637, 2724, 2860
TaiI ACGT 3 cut(s) 1375, 1981, 2237
TaqI TCGA 7 cut(s) 27, 51, 430, 1273, 1381, 2228, 2829
TaqII GACCGA 1 cut(s) 2109
TatI WGTACW 4 cut(s) 98, 822, 988, 1909
TauI GCSGC 3 cut(s) 9, 377, 1588
TfiI GAWTC 4 cut(s) 64, 214, 867, 1253
Tru1I TTAA 3 cut(s) 330, 483, 1889
Tru9I TTAA 3 cut(s) 330, 483, 1889
TscAI CASTG 7 cut(s) 648, 1003, 1498, 1552, 2500, 2642, 2704
TseFI GTSAC 4 cut(s) 898, 2326, 2500, 2699
Tsp45I GTSAC 4 cut(s) 898, 2326, 2500, 2699
TspDTI ATGAA 9 cut(s) 196, 612, 1334, 1686, 1740, 1955, 1990, 2026, 2377
TspGWI ACGGA 3 cut(s) 37, 76, 2461
TspRI CASTG 7 cut(s) 648, 1003, 1498, 1552, 2500, 2642, 2704
VneI GTGCAC 1 cut(s) 662
VpaK11BI GGWCC 7 cut(s) 152, 316, 415, 1011, 2092, 2334, 2655
XagI CCTNNNNNAGG 1 cut(s) 521
XapI RAATTY 2 cut(s) 1361, 2139
XceI RCATGY 3 cut(s) 621, 1088, 1609
XcmI CCANNNNNNNNNTGG 2 cut(s) 970, 1440
XhoI CTCGAG 1 cut(s) 26
XmnI GAANNNNTTC 3 cut(s) 63, 1059, 1503
XspI CTAG 4 cut(s) 1113, 1278, 1851, 2265
ZrmI AGTACT 1 cut(s) 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.