RchiOBHm_Chr7g0182251
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004694
Physical Location & Seq
Forward (+)
3580484 .. 3583285
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16258

Sequence Viewer

Length: 2802 bp
ATGAAGATGAGCTTCCCTGGAATTTGCAACAACTTGGTGGTGGCAAATGTTTCATCAAACAACTTTACTGGTAGGATTGACCAGTACTTTGATGAGTGCTCGAATTTGAAGTACTTGGATTTGAGCACAAACAAATTTAGTGGTGATGTGTGGGTTGGATTCACAAGGCTTAGGGAGTTTTCGGCGTCTGAGAATTACCTCAGTGGGGCTATTTTGCCTTCTATTTTCAATGGCAGTTGTAACTTGGTGATTTTGGACCTGTCACTGAACAATTTCTCTGGTGGAGTTCCAAAGGAGATTTCAAATTGCCGGAATTTGGTTTCGTTGAATGTGTGGGGGAACAAGTTCACAGGGCAAATTCCATCTGAGATTGGAAGCATTTCGAGTCTGCAGTCTTTGTTGTTTGGCAATAACAGTTTTTCTAGAGTGATCCCGGAAGCCCTTTTGAATTTGAGCAACTTGGTGTTCTTGGATTTGAGCAGGAACAATTTTGGAGGAGATATACAGGATATTTTTGGGAGATTTAAGCAGGTGGAGATTCTTGTGCTGCACTCAAATTCATACACAGGTGGTATAAATACATCTGGAATTCTCCAGCTACCTAATGTTTATCGGTTAGATCTCAGTAACAACAATTTTACCGGTTCTTTACCGGTTGAAATTGCTAAAATGCCAAGCTTGAAGTACTTGTTCCTTGCCCACAATCAATTCAGTGGAACCATACCACCAGAGTATGGAAATTGGCAACAGCTCCAAGCACTGGACCTTTCTTTCAACAGACTAACCGGGTCAATCCCCGCCACCTTAGGAAAGTTGAGATCTCTCTTGTGGTTGATGCTTGCCAATAATTCTCTAACAGGTCCGATTCCTAGGGAGTTGGGGAATTGTACTAGTTTGTTGTGGCTGAACCTTTCCAACAACAAGCTCAATGGAACAATCCCATCTGAGTTGACGAATATTGGGACAAATGCAGGTCCAACATTTGAATCAAACAGTGATATCAAGTATGAGATTGTAGCTGGATCCGGGGAGTGCTTAGCAATGAAGAGATGGATTCCAGCAGACTACCCTCCATTCAGTTTTGTGTACACAATCCTCACAAGGAAGAGTTGTCGAAGTATATGGGATCGGCTACTTAAAGGAAATGGCCTTTTTCCCATATGTGTTGCTGGTTCTGCTGTAAGGACACTGCAAATCTCAGGTTATGTACAACTGAGTGGAAACCAGCTTTCCGGTCAGGTCCCTCCAGAGATTGGCGAGATGCGCAATTTTAGTATGATGAATTTGGCTGTCAATCAATTAAGTGGAGAACTCCCTGCCGGTATTGGACAATTGCCACTCGTAGTGTTTAACCTCAGTAAGAACAGTTTCTCAGGCGAAATCCCAATGGAAATTGGGAACAGTACGTGTATGCAGAATCTTGATTTGTCCTACAATAACTTTTCTGGTACATTTCCTGTAAGCTTAAACAGCTTGACTGAGCTAAGCAAGTTCAATATTTCATACAATCCACTCATCTCTGGTGTTATTCCCTCAAGTGGCCAATTGGCAACGTTTGAAAAGGAGTCCTACCTTGGTGACCCCCTCCTTACTCTTCCGAGTTTTATCACCAACTCCCCGAATAATTCAACAAACAAGACAACTGGAAACCAAAAAAAGCCTGCAAAGCTTGGTGCTAATTTGGTGTTTCTAGTGCTAGTACTGACTTTCTTGATATGTGGAATCTTTTCACTTACGATGTGCTTATTTATGAAGAGCCCAGCAGATGAACCACAAGGGTATCTGCTGCCACACAACAAATACCGGCATGACCTTGCATCAAGTTCAAACTCTTCATCACCTTGGCTATCGGATACTGTCAAGGTCATTCGACTGGACAAAACAGCTTTCACACATGCTGACATTTTGAAGGCCACAGGCAATTTTTCAGAAGGGAGGATCATAGGGAGGGGAGGGTTTGGAACAGTGTACCAAGGAGTGTTGCCTGATGGGAGAGAAGTAGCAGTGAAGAAGCTTCAAAGAGAAGGACTTGAAGGTGAAAGGGAATTCAGAGCTGAAATGGAAGTTCTTACTGGAAATGGCTTTGGTTGGCCTCACCCAAATCTTGTGCAACTTCATGGTTGGTGTCATGATGGTTCAGAGAAAATATTAGTCTATGAGTACATGGAAGGTGGGAGCTTGGAGGATATTATATGTGACAAAGTAAGACTAAGATGGCGCAGACGGATTGATGTAGCAGTTGATGTGGCTCGAGCCTTGGTGTTTCTACACCATGAGTGCTTCCCTCCTATCGTGCACCGTGATGTGAAGGCTAGCAATGTGCTGCTAGATAAGGATGGGAAGGCAAGAGTGACAGATTTTGGGCTGGCTAGAATTGTTGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCTGGAACTGTTGGTTATGTTGCACCAGAATATGGGCAGACATGGCAAGCCACAACGAAAGGCGATGTGTACAGTTATGGAGTCCTAGCAATGGAACTGGCAACTGGGAGGAGAGCAGTGGATGGAGGAGAGGAGTGTCTAGTGGAATGGGCCAGAAGGGTGATGGGAAATGGACGGCTAGGATTCAATCGTTCTGTGATGCCGGTTGTGCTTATGGGGTCTGGACTAGTTGATGGGGCAGAAGAGATGTGTGAGTTACTAAGGGTTGGCATAAAGTGCACAGCTGAGGCACCACAGTCTAGACCAAACATGAAGGAAGTTCTAGCTATGCTGATAAAGATAGCCAATATCCAAGGGGATTTTAGTTGTGGTTCTCCTCCTTCATTTTGA

Protein Analysis

933

Amino Acids

102.11

Weight (kDa)

6.5

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 177 - 237 1.8e-08 Leucine rich repeat
LRR_14 PF23598 219 - 307 1.1e-08 Leucine-rich repeat region
LRR_8 PF13855 250 - 309 1.1e-08 Leucine rich repeat
LRR_14 PF23598 402 - 502 7.9e-06 Leucine-rich repeat region
Pkinase PF00069 643 - 912 6.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 645 - 914 1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 520
Acc16I TGCGCA 1 cut(s) 1265
Acc36I ACCTGC 2 cut(s) 520, 962
AccB1I GGYRCC 1 cut(s) 2701
AccB7I CCANNNNNTGG 4 cut(s) 734, 760, 1253, 2444
AciI CCGC 1 cut(s) 798
AclI AACGTT 1 cut(s) 1553
AclWI GGATC 5 cut(s) 424, 1017, 1030, 1134, 1946
AcoI YGGCCR 1 cut(s) 1540
AcyI GRCGYC 1 cut(s) 185
AfiI CCNNNNNNNGG 8 cut(s) 205, 316, 734, 760, 1253, 1538, 2444, 2503
AflIII ACRYGT 1 cut(s) 1406
AgeI ACCGGT 2 cut(s) 641, 652
AhlI ACTAGT 2 cut(s) 890, 2638
AjnI CCWGG 1 cut(s) 16
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AleI CACNNNNGTG 1 cut(s) 2408
Alw21I GWGCWC 5 cut(s) 101, 128, 2298, 2405, 2693
Alw44I GTGCAC 2 cut(s) 2294, 2689
AlwI GGATC 5 cut(s) 424, 1017, 1030, 1134, 1946
Ama87I CYCGRG 1 cut(s) 2250
AoxI GGCC 5 cut(s) 1147, 1540, 1911, 2090, 2562
ApaLI GTGCAC 2 cut(s) 2294, 2689
ApeKI GCWGC 3 cut(s) 547, 1786, 2323
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AsiGI ACCGGT 2 cut(s) 641, 652
Asp700I GAANNNNTTC 5 cut(s) 272, 344, 379, 1367, 1726
AspA2I CCTAGG 1 cut(s) 869
AspLEI GCGC 2 cut(s) 1266, 2220
AspS9I GGNCC 6 cut(s) 256, 763, 860, 974, 1240, 2562
AsuC2I CCSGG 3 cut(s) 434, 787, 1027
AsuHPI GGTGA 8 cut(s) 155, 259, 1589, 1600, 1830, 2048, 2087, 2584
AsuNHI GCTAGC 1 cut(s) 2312
AvaI CYCGRG 1 cut(s) 2250
AvaII GGWCC 5 cut(s) 256, 763, 860, 974, 1240
AvrII CCTAGG 1 cut(s) 869
AxyI CCTNAGG 1 cut(s) 805
BaeGI GKGCMC 2 cut(s) 2298, 2693
BaeI ACNNNNGTAYC 2 cut(s) 1763, 1796
BalI TGGCCA 1 cut(s) 1542
BamHI GGATCC 1 cut(s) 1022
BanI GGYRCC 1 cut(s) 2701
BanII GRGCYC 1 cut(s) 1760
Bbv12I GWGCWC 5 cut(s) 101, 128, 2298, 2405, 2693
BbvCI CCTCAGC 1 cut(s) 2697
BbvI GCAGC 3 cut(s) 534, 1773, 2310
BceAI ACGGC 1 cut(s) 2603
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 1846
BcnI CCSGG 3 cut(s) 434, 787, 1027
BcuI ACTAGT 2 cut(s) 890, 2638
BfmI CTRYAG 1 cut(s) 389
BfuAI ACCTGC 2 cut(s) 520, 962
BfuI GTATCC 1 cut(s) 1846
BglII AGATCT 2 cut(s) 619, 818
BisI GCNGC 3 cut(s) 548, 1787, 2324
BlnI CCTAGG 1 cut(s) 869
BlpI GCTNAGC 2 cut(s) 1036, 1484
BlsI GCNGC 3 cut(s) 549, 1788, 2325
BmcAI AGTACT 4 cut(s) 86, 113, 686, 1701
Bme1390I CCNGG 4 cut(s) 18, 434, 787, 1027
Bme18I GGWCC 5 cut(s) 256, 763, 860, 974, 1240
BmeT110I CYCGRG 1 cut(s) 2250
BmgT120I GGNCC 6 cut(s) 256, 763, 860, 974, 1240, 2562
BmiI GGNNCC 4 cut(s) 718, 1024, 1242, 2703
BmrFI CCNGG 4 cut(s) 18, 434, 787, 1027
BmrI ACTGGG 1 cut(s) 2526
BmsI GCATC 5 cut(s) 825, 1251, 1826, 2371, 2601
BmtI GCTAGC 1 cut(s) 2316
BmuI ACTGGG 1 cut(s) 2526
BpmI CTGGAG 2 cut(s) 578, 1230
Bpu10I CCTNAGC 2 cut(s) 170, 2697
Bpu1102I GCTNAGC 2 cut(s) 1036, 1484
BpuEI CTTGAG 1 cut(s) 1519
BpuMI CCSGG 3 cut(s) 434, 787, 1027
BsaAI YACGTR 1 cut(s) 1407
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 8 cut(s) 16, 869, 1026, 1573, 1841, 1972, 2256, 2764
BsaWI WCCGGW 3 cut(s) 641, 652, 1232
Bsc4I CCNNNNNNNGG 8 cut(s) 205, 316, 734, 760, 1253, 1538, 2444, 2503
Bse118I RCCGGY 5 cut(s) 641, 652, 1319, 1803, 2614
Bse1I ACTGG 8 cut(s) 73, 82, 765, 1648, 1878, 2077, 2514, 2521
Bse21I CCTNAGG 1 cut(s) 805
Bse3DI GCAATG 3 cut(s) 1047, 2323, 2508
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 8 cut(s) 16, 869, 1026, 1573, 1841, 1972, 2256, 2764
BseGI GGATG 2 cut(s) 2341, 2539
BseLI CCNNNNNNNGG 8 cut(s) 205, 316, 734, 760, 1253, 1538, 2444, 2503
BseMI GCAATG 3 cut(s) 1047, 2323, 2508
BseNI ACTGG 8 cut(s) 73, 82, 765, 1648, 1878, 2077, 2514, 2521
BseRI GAGGAG 5 cut(s) 510, 2536, 2553, 2558, 2778
BseSI GKGCMC 2 cut(s) 2298, 2693
BseXI GCAGC 3 cut(s) 534, 1773, 2310
BseYI CCCAGC 1 cut(s) 1759
BsgI GTGCAG 1 cut(s) 533
BshFI GGCC 5 cut(s) 1149, 1542, 1913, 2092, 2564
BshNI GGYRCC 1 cut(s) 2701
BshTI ACCGGT 2 cut(s) 641, 652
BsiHKAI GWGCWC 5 cut(s) 101, 128, 2298, 2405, 2693
BsiHKCI CYCGRG 1 cut(s) 2250
BslFI GGGAC 2 cut(s) 976, 1226
BslI CCNNNNNNNGG 8 cut(s) 205, 316, 734, 760, 1253, 1538, 2444, 2503
BsmFI GGGAC 2 cut(s) 976, 1226
BsnI GGCC 5 cut(s) 1149, 1542, 1913, 2092, 2564
BsoBI CYCGRG 1 cut(s) 2250
Bsp1286I GDGCHC 6 cut(s) 101, 128, 1760, 2298, 2405, 2693
Bsp1407I TGTACA 3 cut(s) 1086, 1207, 2481
Bsp143I GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
Bsp1720I GCTNAGC 2 cut(s) 1036, 1484
BspACI CCGC 1 cut(s) 798
BspANI GGCC 5 cut(s) 1149, 1542, 1913, 2092, 2564
BspHI TCATGA 1 cut(s) 2128
BspLI GGNNCC 4 cut(s) 718, 1024, 1242, 2703
BspMAI CTGCAG 1 cut(s) 393
BspMI ACCTGC 2 cut(s) 520, 962
BspOI GCTAGC 1 cut(s) 2316
BspPI GGATC 5 cut(s) 424, 1017, 1030, 1134, 1946
BspQI GCTCTTC 1 cut(s) 1748
BspT107I GGYRCC 1 cut(s) 2701
BsrDI GCAATG 3 cut(s) 1047, 2323, 2508
BsrFI RCCGGY 5 cut(s) 641, 652, 1319, 1803, 2614
BsrGI TGTACA 3 cut(s) 1086, 1207, 2481
BsrI ACTGG 8 cut(s) 73, 82, 765, 1648, 1878, 2077, 2514, 2521
BssAI RCCGGY 5 cut(s) 641, 652, 1319, 1803, 2614
BssECI CCNNGG 8 cut(s) 16, 869, 1026, 1573, 1841, 1972, 2256, 2764
BssMI GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 6 cut(s) 869, 1573, 1841, 1972, 2256, 2764
Bst2UI CCWGG 1 cut(s) 18
Bst6I CTCTTC 6 cut(s) 1040, 1100, 1599, 1748, 1837, 2649
BstACI GRCGYC 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 2688
BstAUI TGTACA 3 cut(s) 1086, 1207, 2481
BstBAI YACGTR 1 cut(s) 1407
BstC8I GCNNGC 5 cut(s) 840, 1662, 2314, 2367, 2460
BstEII GGTNACC 1 cut(s) 1577
BstF5I GGATG 2 cut(s) 2341, 2539
BstHHI GCGC 2 cut(s) 1266, 2220
BstKTI GATC 6 cut(s) 432, 622, 821, 1025, 1129, 1941
BstMBI GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
BstMWI GCNNNNNNNGC 7 cut(s) 1175, 1263, 1470, 1666, 2455, 2620, 2688
BstNI CCWGG 1 cut(s) 18
BstNSI RCATGY 1 cut(s) 1898
BstPI GGTNACC 1 cut(s) 1577
BstSCI CCNGG 4 cut(s) 16, 432, 785, 1025
BstSFI CTRYAG 1 cut(s) 389
BstSLI GKGCMC 2 cut(s) 2298, 2693
BstV1I GCAGC 3 cut(s) 534, 1773, 2310
BstX2I RGATCY 3 cut(s) 619, 818, 1022
BstYI RGATCY 3 cut(s) 619, 818, 1022
Bsu36I CCTNAGG 1 cut(s) 805
BsuI GTATCC 1 cut(s) 1846
BsuRI GGCC 5 cut(s) 1149, 1542, 1913, 2092, 2564
BtgZI GCGATG 1 cut(s) 2490
BtsCI GGATG 2 cut(s) 2341, 2539
BtsI GCAGTG 3 cut(s) 1187, 2010, 2535
BtsIMutI CAGTG 9 cut(s) 208, 263, 718, 758, 1000, 1187, 1971, 2010, 2535
BveI ACCTGC 2 cut(s) 520, 962
Cac8I GCNNGC 5 cut(s) 840, 1662, 2314, 2367, 2460
CciI TCATGA 1 cut(s) 2128
CfoI GCGC 2 cut(s) 1266, 2220
Cfr10I RCCGGY 5 cut(s) 641, 652, 1319, 1803, 2614
Cfr13I GGNCC 6 cut(s) 256, 763, 860, 974, 1240, 2562
CseI GACGC 1 cut(s) 174
CspAI ACCGGT 2 cut(s) 641, 652
CspCI CAANNNNNGTGG 2 cut(s) 121, 156
CviAII CATG 9 cut(s) 1808, 1895, 2117, 2129, 2164, 2273, 2396, 2454, 2722
DpnI GATC 6 cut(s) 431, 621, 820, 1024, 1128, 1940
DpnII GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
EaeI YGGCCR 1 cut(s) 1540
Eam1104I CTCTTC 6 cut(s) 1040, 1100, 1599, 1748, 1837, 2649
EarI CTCTTC 6 cut(s) 1040, 1100, 1599, 1748, 1837, 2649
Eco130I CCWWGG 6 cut(s) 869, 1573, 1841, 1972, 2256, 2764
Eco24I GRGCYC 1 cut(s) 1760
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 5 cut(s) 256, 763, 860, 974, 1240
Eco81I CCTNAGG 1 cut(s) 805
Eco88I CYCGRG 1 cut(s) 2250
Eco91I GGTNACC 1 cut(s) 1577
EcoO109I RGGNCCY 1 cut(s) 1240
EcoO65I GGTNACC 1 cut(s) 1577
EcoRI GAATTC 2 cut(s) 588, 2045
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 1 cut(s) 1000
EcoT14I CCWWGG 6 cut(s) 869, 1573, 1841, 1972, 2256, 2764
EcoT38I GRGCYC 1 cut(s) 1760
ErhI CCWWGG 6 cut(s) 869, 1573, 1841, 1972, 2256, 2764
FaeI CATG 9 cut(s) 1811, 1898, 2120, 2132, 2167, 2276, 2399, 2457, 2725
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 2 cut(s) 976, 1226
FatI CATG 9 cut(s) 1807, 1894, 2116, 2128, 2163, 2272, 2395, 2453, 2721
FauI CCCGC 1 cut(s) 805
FauNDI CATATG 1 cut(s) 1160
Fnu4HI GCNGC 3 cut(s) 548, 1787, 2324
FokI GGATG 2 cut(s) 2348, 2546
FriOI GRGCYC 1 cut(s) 1760
Fsp4HI GCNGC 3 cut(s) 548, 1787, 2324
FspI TGCGCA 1 cut(s) 1265
GlaI GCGC 2 cut(s) 1265, 2219
GluI GCNGC 3 cut(s) 548, 1787, 2324
GsaI CCCAGC 1 cut(s) 1763
GsuI CTGGAG 2 cut(s) 578, 1230
HaeIII GGCC 5 cut(s) 1149, 1542, 1913, 2092, 2564
HgaI GACGC 1 cut(s) 174
HhaI GCGC 2 cut(s) 1266, 2220
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 9 cut(s) 1811, 1898, 2120, 2132, 2167, 2276, 2399, 2457, 2725
Hin6I GCGC 2 cut(s) 1264, 2218
HinP1I GCGC 2 cut(s) 1264, 2218
HincII GTYRAC 1 cut(s) 951
HindII GTYRAC 1 cut(s) 951
HindIII AAGCTT 4 cut(s) 676, 1462, 1667, 2012
HphI GGTGA 8 cut(s) 155, 259, 1589, 1600, 1830, 2048, 2087, 2584
Hpy166II GTNNAC 8 cut(s) 348, 951, 1087, 1089, 1969, 2296, 2482, 2691
Hpy188I TCNGA 9 cut(s) 190, 367, 864, 946, 1599, 1852, 1930, 2051, 2140
Hpy188III TCNNGA 8 cut(s) 423, 585, 1247, 1421, 1711, 2129, 2634, 2712
Hpy8I GTNNAC 8 cut(s) 348, 951, 1087, 1089, 1969, 2296, 2482, 2691
HpyCH4IV ACGT 2 cut(s) 1406, 1553
HpyF10VI GCNNNNNNNGC 7 cut(s) 1175, 1263, 1470, 1666, 2455, 2620, 2688
HpySE526I ACGT 2 cut(s) 1406, 1553
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 9 cut(s) 1811, 1898, 2120, 2132, 2167, 2276, 2399, 2457, 2725
HspAI GCGC 2 cut(s) 1264, 2218
Kzo9I GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
LguI GCTCTTC 1 cut(s) 1748
LmnI GCTCC 2 cut(s) 756, 2175
Lsp1109I GCAGC 3 cut(s) 534, 1773, 2310
LweI GCATC 5 cut(s) 825, 1251, 1826, 2371, 2601
MaeII ACGT 2 cut(s) 1406, 1553
MaeIII GTNAC 7 cut(s) 239, 261, 626, 1577, 2195, 2350, 2667
MalI GATC 6 cut(s) 431, 621, 820, 1024, 1128, 1940
MboI GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
MboII GAAGA 8 cut(s) 16, 1057, 1117, 1586, 1765, 1824, 2020, 2666
MfeI CAATTG 2 cut(s) 1331, 1544
MflI RGATCY 3 cut(s) 619, 818, 1022
MhlI GDGCHC 6 cut(s) 101, 128, 1760, 2298, 2405, 2693
MlsI TGGCCA 1 cut(s) 1542
MluNI TGGCCA 1 cut(s) 1542
MlyI GAGTC 3 cut(s) 394, 1574, 2502
MmeI TCCRAC 3 cut(s) 136, 939, 1001
Mox20I TGGCCA 1 cut(s) 1542
MroXI GAANNNNTTC 5 cut(s) 272, 344, 379, 1367, 1726
MscI TGGCCA 1 cut(s) 1542
MseI TTAA 5 cut(s) 525, 1137, 1301, 1350, 1466
MslI CAYNNNNRTG 2 cut(s) 2301, 2408
Msp20I TGGCCA 1 cut(s) 1542
MspA1I CMGCKG 1 cut(s) 2696
MspR9I CCNGG 4 cut(s) 18, 434, 787, 1027
MunI CAATTG 2 cut(s) 1331, 1544
MvaI CCWGG 1 cut(s) 18
MwoI GCNNNNNNNGC 7 cut(s) 1175, 1263, 1470, 1666, 2455, 2620, 2688
NciI CCSGG 3 cut(s) 434, 787, 1027
NdeI CATATG 1 cut(s) 1160
NdeII GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
NheI GCTAGC 1 cut(s) 2312
NlaIII CATG 9 cut(s) 1811, 1898, 2120, 2132, 2167, 2276, 2399, 2457, 2725
NlaIV GGNNCC 4 cut(s) 718, 1024, 1242, 2703
NmuCI GTSAC 4 cut(s) 261, 1577, 2195, 2350
NsbI TGCGCA 1 cut(s) 1265
NspI RCATGY 1 cut(s) 1898
OliI CACNNNNGTG 1 cut(s) 2408
PaeR7I CTCGAG 1 cut(s) 2250
PagI TCATGA 1 cut(s) 2128
PaqCI CACCTGC 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 1748
PdmI GAANNNNTTC 5 cut(s) 272, 344, 379, 1367, 1726
PfeI GAWTC 8 cut(s) 159, 538, 865, 986, 1054, 1417, 1722, 2595
PflMI CCANNNNNTGG 4 cut(s) 734, 760, 1253, 2444
PfoI TCCNGGA 1 cut(s) 432
PinAI ACCGGT 2 cut(s) 641, 652
PkrI GCNGC 3 cut(s) 549, 1788, 2325
PleI GAGTC 3 cut(s) 393, 1573, 2501
PpsI GAGTC 3 cut(s) 393, 1573, 2501
Ppu21I YACGTR 1 cut(s) 1407
PpuMI RGGWCCY 1 cut(s) 1240
Psp1406I AACGTT 1 cut(s) 1553
Psp5II RGGWCCY 1 cut(s) 1240
Psp6I CCWGG 1 cut(s) 16
PspEI GGTNACC 1 cut(s) 1577
PspFI CCCAGC 1 cut(s) 1759
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 4 cut(s) 718, 1024, 1242, 2703
PspPI GGNCC 6 cut(s) 256, 763, 860, 974, 1240, 2562
PspPPI RGGWCCY 1 cut(s) 1240
PspXI VCTCGAGB 1 cut(s) 2250
PstI CTGCAG 1 cut(s) 393
PsuI RGATCY 3 cut(s) 619, 818, 1022
PvuII CAGCTG 1 cut(s) 2696
RseI CAYNNNNRTG 2 cut(s) 2301, 2408
SapI GCTCTTC 1 cut(s) 1748
SaqAI TTAA 5 cut(s) 525, 1137, 1301, 1350, 1466
SatI GCNGC 3 cut(s) 548, 1787, 2324
Sau3AI GATC 6 cut(s) 429, 619, 818, 1022, 1126, 1938
Sau96I GGNCC 6 cut(s) 256, 763, 860, 974, 1240, 2562
ScaI AGTACT 4 cut(s) 86, 113, 686, 1701
SchI GAGTC 3 cut(s) 394, 1574, 2502
ScrFI CCNGG 4 cut(s) 18, 434, 787, 1027
SduI GDGCHC 6 cut(s) 101, 128, 1760, 2298, 2405, 2693
SfaNI GCATC 5 cut(s) 825, 1251, 1826, 2371, 2601
SfcI CTRYAG 1 cut(s) 389
Sfr274I CTCGAG 1 cut(s) 2250
SinI GGWCC 5 cut(s) 256, 763, 860, 974, 1240
SlaI CTCGAG 1 cut(s) 2250
SmiMI CAYNNNNRTG 2 cut(s) 2301, 2408
SmlI CTYRAG 2 cut(s) 1534, 2250
SmoI CTYRAG 2 cut(s) 1534, 2250
SpeI ACTAGT 2 cut(s) 890, 2638
SsiI CCGC 1 cut(s) 798
SspI AATATT 3 cut(s) 958, 1498, 2148
StyD4I CCNGG 4 cut(s) 16, 432, 785, 1025
StyI CCWWGG 6 cut(s) 869, 1573, 1841, 1972, 2256, 2764
TaiI ACGT 2 cut(s) 1409, 1556
TaqI TCGA 5 cut(s) 101, 383, 1114, 1870, 2251
TatI WGTACW 9 cut(s) 84, 111, 684, 887, 1086, 1207, 1699, 2160, 2481
TfiI GAWTC 8 cut(s) 159, 538, 865, 986, 1054, 1417, 1722, 2595
Tru1I TTAA 5 cut(s) 525, 1137, 1301, 1350, 1466
Tru9I TTAA 5 cut(s) 525, 1137, 1301, 1350, 1466
TscAI CASTG 9 cut(s) 208, 270, 718, 765, 1000, 1194, 1971, 2010, 2535
TseFI GTSAC 4 cut(s) 261, 1577, 2195, 2350
TseI GCWGC 3 cut(s) 547, 1786, 2323
Tsp45I GTSAC 4 cut(s) 261, 1577, 2195, 2350
TspGWI ACGGA 1 cut(s) 2239
TspRI CASTG 9 cut(s) 208, 270, 718, 765, 1000, 1194, 1971, 2010, 2535
Van91I CCANNNNNTGG 4 cut(s) 734, 760, 1253, 2444
VneI GTGCAC 2 cut(s) 2294, 2689
VpaK11BI GGWCC 5 cut(s) 256, 763, 860, 974, 1240
XbaI TCTAGA 2 cut(s) 422, 2711
XceI RCATGY 1 cut(s) 1898
XcmI CCANNNNNNNNNTGG 1 cut(s) 2572
XhoI CTCGAG 1 cut(s) 2250
XmaJI CCTAGG 1 cut(s) 869
XmnI GAANNNNTTC 5 cut(s) 272, 344, 379, 1367, 1726
ZrmI AGTACT 4 cut(s) 86, 113, 686, 1701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.