FvH4_5g14410
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004694
Physical Location & Seq
Reverse (-)
8158456 .. 8162464
4009 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14410.t1

Sequence Viewer

Length: 3306 bp
ATGTACAGCAACTCGAAAACTCATTCATGGCGCTCAGTGTGGTGTTGTTCTTCCATATTCTCACTCATCATCATGATCACAGCTGAAGTTGTAGCTGGAGATTCTCTAAACACAGACATAGAAGTTCTGCTGAGCCTTAAAGCTTTTCTTCAAGAACATAACAAAGTAAACCAAGGCAGATACTCAGAATGGAACCAGAGCAGCATTAGCCCCTGTGATTGGCCTAGAGTTAAATGCAATGGTGACAGAAGAGTTACCGGAATATATCTCACTGACAGCAAAATCACAGGTCAGATGTTCGGAAACTTCTCGGCTTTGACACAGCTTTCGGAGCTGGACTTATCGGATAACACTCTCGGTGGAGCTATCCCGGAGGACTTGAGTCAATGCCAGAGCCTCAAGTACTTGAATTTGTCACATAACATTCTTGAAGGTGAGCTGAGATTGCAGGGGTTGAGTCAATTGGAGGTTCTTGATTTGACTGTGAATAGGCTTTATGGGGATATGAAGATGACCTTCCCTGGAATTTGCAAGAACTTGGTGGTGGCAAATGTGTCCTCCAATAACTTCACTGGTAGAATTGACCAGTACTTTGATGAGTGCTATAATTTGCAGTATTTGGATCTGAGCTCAAACAATTTCAGTGGTGATGTGTGGGTTGGATTCACAAGGCTTAGGGAGTTTTCTGCGTCTGAGAATGATCTGAGTGGGGCTATTTTGCCTTCTATTTTCAATGGCACTTGTAGCTTGGTGAGTTTAGACCTGTCAGTAAACAATTTTTCTGGTGACGTTCCAAAGGAGATATCAAATTGCCGGAATTTGGTTACATTGAATCTGTGGGGAAACAAATTTACAGGGCTGATTCCATCTGAGATTGGAAGTCTCTGGAGTCTGGAGACTTTGTTCTTGGGAAATAACAGTTTTTCTAGAGTGATCCCGGAAGCTCTTTTGAATTTGAGCAACTTGGTGTTCTTGGATTTGAGCAGGAACAATTTTGGTGGAGATATACAAGAGATTTTTGGGAGATTTACGCAGGTGAAGTTTCTTGTGCTGCACTCAAACTCATACATAGGTGGTATATATTCATCTGGAATTCTCAAGCTACCTAATATATCTCGGTTGGATCTCAGTCACAACCACTTTACAGGTTCTTTACCAGTTGAAATTGCTGAAATGTCAAGCTTGAAGTACTTGTTCCTCGCCTACAATGAATTCAATGGAACTATACCACCAGAGTATGGAAATTTGACACAGCTCCAAGCACTGGACCTTTCTTTCAACAGCCTAACTGGGTCAATTCCTGCCACCATAGGAAAGTTAAGGTCTCTTTTGTGGTTGATGCTTGCTGATAATTCTCTAACAGGTCCAATTCCTAGGGAGTTAGGGAATTGTACTAGCTTGTTGTGGCTGAACCTTTCCAACAACAAGCTCAATGGAACAATCCCATATGAGTTGATGAATATTGGGACAAATCCTGGTCCAACATTTGAATCAAACAATGAGAACGAGGATCAGATCGTAGCTGGATCCGGGGAGTGCTTAGCAATGAAGAGATGGATTCCAGCAGACTACCCTCCATTCAGTTTTGTGTACACAATCCTAACCAGGAAAAGTTGTCGAAGCATATGGGATCGACTACTTAAAGGAAATGGCCTTTTCCCAATATGTGTAGCTGGTTCTGCAGTAAGGACATTGCAAATCTCAGGTTATGTGCAACTGAGTGGGAACCAGCTTTCAGGTCAGGTCCCTCCAGAGATTGGTGAGATGCACAATTTTAGTATGATCAATTTGGCTTTCAATCAATTAACTGGAGAACTCCCTGCAGGGATTGGACAAATTCCACTTGTAGTGTTTAACCTTACCGATAACAGTTTCTCGGGCGAAATCCCAATGGAAATTGGTAACATCATGTGTATGCAGAATCTTGATTTGTCCTACAATAACTTTTCTGGTACATTTCCTGTAAGCTTAAACAGCTTGCATGAGCTAAGCAAGTTTAATGTCTCATACAATCCACTCATATCTGGTGTTATTCCTTCAAGTGGACAGTTGGCAACGTTTGAAAAGGAGTCCTACCTTGGTGACCCTCTACTTATCCTTCCGAAGTTTCTCACTGACTCCACAGATCACTCAAGAAACAAACCAACTGAAAACCTGAAAAAGCCTGCAAAGATTGCTACTTATTTGGTGTTTTTAGGGCTAGTACTGACATTCTTGATATGTGGAGTCTTTTCACTTATAATGTGCTTACATATGAAGGGGCCAGCAGATGAGCCACAAGGGTATCTGCTGTCACACGGTAAATACCGGCATGACTTTGCATCAAGCTCAAACTCTTCATCACCTTGGCTATCGGATACTGTCAAAGTCATTCGACTGGACAAAACAGCTTTCACACATGCTGACATTTTGAAGGCCACAGGAAACTTTTCAGAAGGGAGGATCATAGGGAGGGGAGGGTTTGGAACTGTGTACCAAGGAGTGTTGCCTGATGGTAGAGAAGTAGCAGTGAAGAAGCTTCAAAGAGAAGGACTTGAAGGCGAAAGGGAATTTCGAGCTGAGATGGAAGTTCTTACTGGAAATGGGTTTGGTTGGCCTCACCCAAATCTTGTGCAACTTCATGGCTGGTGTCATGATGGTTCAGAGAAAATATTAGTCTATGAGTACATGGAAGGTGGGAGCTTGGAGGATATTATATGTGACAAAGTAAGACTAAGATGGCGCAGGCGGATTGATATAGCAGTGGATGTGGCTAGAGCCTTGATGTTTCTACACCACGAGTGCTACCCTGCTATTGTACACCGTGATGTGAAGGCTAGCAATGTGCTGCTAGATAAGGATGGGAAGGCAAGAGTAACAGATTTTGGGCTGGCTAGAATTGTTGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCTGGGACTGTTGGTTATGTTGCACCAGAATATGGGCAGACATGGCAAGCCACAACGAAAGGCGATGTGTACAGTTATGGAGTCCTAGCAATGGAACTGGCAACTGGGAGGAGAGCAGTGGATGGAGGAGAGGAGTGTCTAGTGGAATGGGCCAGAAGGGTGATGGGAAATGGCCGGCTAGGATTCAATCGTTCTGTGATGCCGGTTATGCTTATGGGGTCTGGACTAGTTGATGGGGCTGAAGAGATGTGTGAGTTACTAAGGGTTGGCATAAAGTGCACAGCTGAGGCACCACAGTCCAGACCAAACATGAAGGAAGTTTTAGCTATGCTTATAAAGATAGTCAATCTCCAAGGGGATTTTAGTTATGGTTCTCCTCCTTCATTTTGA

Protein Analysis

1102

Amino Acids

121.56

Weight (kDa)

5.48

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 80 1.2e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 84 - 141 1.6e-07 Leucine rich repeat
LRR_8 PF13855 272 - 332 2.2e-08 Leucine rich repeat
LRR_8 PF13855 346 - 404 3.4e-06 Leucine rich repeat
LRR_14 PF23598 388 - 474 2e-09 Leucine-rich repeat region
LRR_8 PF13855 418 - 477 3.3e-08 Leucine rich repeat
Pkinase PF00069 811 - 1080 2.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 813 - 1081 1.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2240, 3251
AarI CACCTGC 1 cut(s) 1024
Acc36I ACCTGC 1 cut(s) 1024
AccB1I GGYRCC 1 cut(s) 3205
AccB7I CCANNNNNTGG 4 cut(s) 1238, 1264, 1757, 2948
AciI CCGC 1 cut(s) 2728
AclI AACGTT 1 cut(s) 2057
AclWI GGATC 8 cut(s) 630, 928, 1131, 1518, 1521, 1534, 1638, 2450
AcoI YGGCCR 1 cut(s) 3088
AcuI CTGAAG 2 cut(s) 105, 3177
AfiI CCNNNNNNNGG 8 cut(s) 219, 820, 1238, 1264, 1757, 2042, 2948, 3007
AhlI ACTAGT 1 cut(s) 3142
AjnI CCWGG 3 cut(s) 520, 1474, 1604
AleI CACNNNNGTG 1 cut(s) 2912
AloI GAACNNNNNNTCC 2 cut(s) 887, 919
Alw21I GWGCWC 3 cut(s) 632, 2909, 3197
Alw26I GTCTC 4 cut(s) 887, 890, 1329, 2008
Alw44I GTGCAC 1 cut(s) 3193
AlwI GGATC 8 cut(s) 630, 928, 1131, 1518, 1521, 1534, 1638, 2450
Ama87I CYCGRG 1 cut(s) 1876
AoxI GGCC 7 cut(s) 221, 1651, 2261, 2415, 2594, 3066, 3088
ApaLI GTGCAC 1 cut(s) 3193
ApeKI GCWGC 3 cut(s) 201, 1051, 2827
ArsI GACNNNNNNTTYG 4 cut(s) 310, 342, 575, 607
Asp700I GAANNNNTTC 1 cut(s) 2428
AspA2I CCTAGG 1 cut(s) 1373
AspLEI GCGC 2 cut(s) 33, 2724
AspS9I GGNCC 6 cut(s) 1267, 1364, 1478, 1744, 2261, 3066
AsuC2I CCSGG 3 cut(s) 371, 938, 1531
AsuNHI GCTAGC 1 cut(s) 2816
AvaI CYCGRG 1 cut(s) 1876
AvaII GGWCC 4 cut(s) 1267, 1364, 1478, 1744
AvrII CCTAGG 1 cut(s) 1373
BaeGI GKGCMC 1 cut(s) 3197
BaeI ACNNNNGTAYC 2 cut(s) 2267, 2300
BamHI GGATCC 1 cut(s) 1526
BanI GGYRCC 1 cut(s) 3205
BanII GRGCYC 1 cut(s) 632
BauI CACGAG 1 cut(s) 2777
Bbv12I GWGCWC 3 cut(s) 632, 2909, 3197
BbvCI CCTCAGC 1 cut(s) 3201
BbvI GCAGC 3 cut(s) 213, 1038, 2814
BciT130I CCWGG 3 cut(s) 522, 1476, 1606
BciVI GTATCC 1 cut(s) 2350
BclI TGATCA 2 cut(s) 75, 1782
BcnI CCSGG 3 cut(s) 371, 938, 1531
BcoDI GTCTC 4 cut(s) 887, 890, 1329, 2008
BcuI ACTAGT 1 cut(s) 3142
BfmI CTRYAG 2 cut(s) 1680, 1821
BfoI RGCGCY 1 cut(s) 34
BfuAI ACCTGC 1 cut(s) 1024
BfuI GTATCC 1 cut(s) 2350
BisI GCNGC 3 cut(s) 202, 1052, 2828
BlnI CCTAGG 1 cut(s) 1373
BlpI GCTNAGC 3 cut(s) 131, 1540, 1988
BlsI GCNGC 3 cut(s) 203, 1053, 2829
BmcAI AGTACT 4 cut(s) 404, 590, 1190, 2205
Bme1390I CCNGG 6 cut(s) 371, 522, 938, 1476, 1531, 1606
Bme18I GGWCC 4 cut(s) 1267, 1364, 1478, 1744
BmeT110I CYCGRG 1 cut(s) 1876
BmgT120I GGNCC 6 cut(s) 1267, 1364, 1478, 1744, 2261, 3066
BmiI GGNNCC 6 cut(s) 194, 1528, 1727, 1746, 2262, 3207
BmrFI CCNGG 6 cut(s) 371, 522, 938, 1476, 1531, 1606
BmrI ACTGGG 2 cut(s) 1299, 3030
BmsI GCATC 5 cut(s) 1329, 1755, 2330, 2875, 3105
BmtI GCTAGC 1 cut(s) 2820
BmuI ACTGGG 2 cut(s) 1299, 3030
BoxI GACNNNNGTC 1 cut(s) 381
BpmI CTGGAG 5 cut(s) 117, 907, 914, 1734, 1830
Bpu10I CCTNAGC 2 cut(s) 674, 3201
Bpu1102I GCTNAGC 3 cut(s) 131, 1540, 1988
BpuEI CTTGAG 4 cut(s) 383, 400, 1082, 2116
BpuMI CCSGG 3 cut(s) 371, 938, 1531
BsaI GGTCTC 1 cut(s) 1329
BsaJI CCNNGG 8 cut(s) 172, 520, 1373, 1530, 2077, 2345, 2476, 3268
BsaWI WCCGGW 1 cut(s) 257
BsaXI ACNNNNNCTCC 2 cut(s) 887, 917
Bsc4I CCNNNNNNNGG 8 cut(s) 219, 820, 1238, 1264, 1757, 2042, 2948, 3007
Bse118I RCCGGY 3 cut(s) 2307, 3090, 3118
Bse3DI GCAATG 5 cut(s) 244, 1551, 1691, 2827, 3012
BseBI CCWGG 3 cut(s) 522, 1476, 1606
BseDI CCNNGG 8 cut(s) 172, 520, 1373, 1530, 2077, 2345, 2476, 3268
BseGI GGATG 3 cut(s) 2752, 2845, 3043
BseLI CCNNNNNNNGG 8 cut(s) 219, 820, 1238, 1264, 1757, 2042, 2948, 3007
BseMI GCAATG 5 cut(s) 244, 1551, 1691, 2827, 3012
BseRI GAGGAG 4 cut(s) 3040, 3057, 3062, 3282
BseSI GKGCMC 1 cut(s) 3197
BseXI GCAGC 3 cut(s) 213, 1038, 2814
BseYI CCCAGC 1 cut(s) 2918
BsgI GTGCAG 1 cut(s) 1037
BshFI GGCC 7 cut(s) 223, 1653, 2263, 2417, 2596, 3068, 3090
BshNI GGYRCC 1 cut(s) 3205
BsiHKAI GWGCWC 3 cut(s) 632, 2909, 3197
BsiHKCI CYCGRG 1 cut(s) 1876
BsiSI CCGG 8 cut(s) 258, 371, 814, 938, 1530, 2308, 3091, 3119
BslFI GGGAC 3 cut(s) 1480, 1730, 2935
BslI CCNNNNNNNGG 8 cut(s) 219, 820, 1238, 1264, 1757, 2042, 2948, 3007
BsmAI GTCTC 4 cut(s) 887, 890, 1329, 2008
BsmFI GGGAC 3 cut(s) 1480, 1730, 2935
BsnI GGCC 7 cut(s) 223, 1653, 2263, 2417, 2596, 3068, 3090
Bso31I GGTCTC 1 cut(s) 1329
BsoBI CYCGRG 1 cut(s) 1876
Bsp1286I GDGCHC 3 cut(s) 632, 2909, 3197
Bsp1407I TGTACA 4 cut(s) 3, 1590, 2797, 2985
Bsp1720I GCTNAGC 3 cut(s) 131, 1540, 1988
BspACI CCGC 1 cut(s) 2728
BspANI GGCC 7 cut(s) 223, 1653, 2263, 2417, 2596, 3068, 3090
BspHI TCATGA 2 cut(s) 72, 2632
BspLI GGNNCC 6 cut(s) 194, 1528, 1727, 1746, 2262, 3207
BspMAI CTGCAG 2 cut(s) 1684, 1825
BspMI ACCTGC 1 cut(s) 1024
BspOI GCTAGC 1 cut(s) 2820
BspPI GGATC 8 cut(s) 630, 928, 1131, 1518, 1521, 1534, 1638, 2450
BspT107I GGYRCC 1 cut(s) 3205
BspTNI GGTCTC 1 cut(s) 1329
BsrDI GCAATG 5 cut(s) 244, 1551, 1691, 2827, 3012
BsrFI RCCGGY 3 cut(s) 2307, 3090, 3118
BsrGI TGTACA 4 cut(s) 3, 1590, 2797, 2985
BssAI RCCGGY 3 cut(s) 2307, 3090, 3118
BssECI CCNNGG 8 cut(s) 172, 520, 1373, 1530, 2077, 2345, 2476, 3268
BssSI CACGAG 1 cut(s) 2777
BssT1I CCWWGG 6 cut(s) 172, 1373, 2077, 2345, 2476, 3268
Bst2BI CACGAG 1 cut(s) 2777
Bst2UI CCWGG 3 cut(s) 522, 1476, 1606
Bst6I CTCTTC 4 cut(s) 244, 1544, 2341, 3153
BstAPI GCANNNNNTGC 2 cut(s) 2174, 3192
BstAUI TGTACA 4 cut(s) 3, 1590, 2797, 2985
BstC8I GCNNGC 9 cut(s) 1344, 1979, 2166, 2265, 2726, 2818, 2871, 2964, 3092
BstEII GGTNACC 1 cut(s) 2081
BstF5I GGATG 3 cut(s) 2752, 2845, 3043
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 2 cut(s) 33, 2724
BstMAI GTCTC 4 cut(s) 887, 890, 1329, 2008
BstNI CCWGG 3 cut(s) 522, 1476, 1606
BstNSI RCATGY 1 cut(s) 2402
BstPAI GACNNNNGTC 1 cut(s) 381
BstPI GGTNACC 1 cut(s) 2081
BstSCI CCNGG 6 cut(s) 369, 520, 936, 1474, 1529, 1604
BstSFI CTRYAG 2 cut(s) 1680, 1821
BstSLI GKGCMC 1 cut(s) 3197
BstV1I GCAGC 3 cut(s) 213, 1038, 2814
BstX2I RGATCY 3 cut(s) 622, 1123, 1526
BstYI RGATCY 3 cut(s) 622, 1123, 1526
BsuI GTATCC 1 cut(s) 2350
BsuRI GGCC 7 cut(s) 223, 1653, 2263, 2417, 2596, 3068, 3090
BtgZI GCGATG 1 cut(s) 2994
BtsCI GGATG 3 cut(s) 2752, 2845, 3043
BtsI GCAGTG 3 cut(s) 2514, 2748, 3039
BtsIMutI CAGTG 9 cut(s) 42, 270, 570, 649, 1262, 2112, 2514, 2748, 3039
BveI ACCTGC 1 cut(s) 1024
Cac8I GCNNGC 9 cut(s) 1344, 1979, 2166, 2265, 2726, 2818, 2871, 2964, 3092
CciI TCATGA 2 cut(s) 72, 2632
CfoI GCGC 2 cut(s) 33, 2724
Cfr10I RCCGGY 3 cut(s) 2307, 3090, 3118
Cfr13I GGNCC 6 cut(s) 1267, 1364, 1478, 1744, 2261, 3066
CseI GACGC 1 cut(s) 678
CspCI CAANNNNNGTGG 4 cut(s) 625, 660, 979, 1014
EaeI YGGCCR 1 cut(s) 3088
Eam1104I CTCTTC 4 cut(s) 244, 1544, 2341, 3153
EarI CTCTTC 4 cut(s) 244, 1544, 2341, 3153
EciI GGCGGA 1 cut(s) 2743
Ecl136II GAGCTC 1 cut(s) 630
Eco130I CCWWGG 6 cut(s) 172, 1373, 2077, 2345, 2476, 3268
Eco24I GRGCYC 1 cut(s) 632
Eco31I GGTCTC 1 cut(s) 1329
Eco32I GATATC 1 cut(s) 804
Eco47I GGWCC 4 cut(s) 1267, 1364, 1478, 1744
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 2 cut(s) 105, 3177
Eco88I CYCGRG 1 cut(s) 1876
Eco91I GGTNACC 1 cut(s) 2081
EcoICRI GAGCTC 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 1744
EcoO65I GGTNACC 1 cut(s) 2081
EcoRI GAATTC 2 cut(s) 1092, 1211
EcoRII CCWGG 3 cut(s) 520, 1474, 1604
EcoRV GATATC 1 cut(s) 804
EcoT14I CCWWGG 6 cut(s) 172, 1373, 2077, 2345, 2476, 3268
EcoT38I GRGCYC 1 cut(s) 632
ErhI CCWWGG 6 cut(s) 172, 1373, 2077, 2345, 2476, 3268
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 500, 532
FaqI GGGAC 3 cut(s) 1480, 1730, 2935
FauNDI CATATG 3 cut(s) 1447, 1625, 2253
FbaI TGATCA 2 cut(s) 75, 1782
Fnu4HI GCNGC 3 cut(s) 202, 1052, 2828
FokI GGATG 3 cut(s) 2759, 2852, 3050
FriOI GRGCYC 1 cut(s) 632
Fsp4HI GCNGC 3 cut(s) 202, 1052, 2828
GlaI GCGC 2 cut(s) 32, 2723
GluI GCNGC 3 cut(s) 202, 1052, 2828
GsaI CCCAGC 1 cut(s) 2922
GsuI CTGGAG 5 cut(s) 117, 907, 914, 1734, 1830
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 7 cut(s) 223, 1653, 2263, 2417, 2596, 3068, 3090
HapII CCGG 8 cut(s) 258, 371, 814, 938, 1530, 2308, 3091, 3119
HgaI GACGC 1 cut(s) 678
HhaI GCGC 2 cut(s) 33, 2724
Hin6I GCGC 2 cut(s) 31, 2722
HinP1I GCGC 2 cut(s) 31, 2722
HindIII AAGCTT 4 cut(s) 141, 1180, 1966, 2516
HpaII CCGG 8 cut(s) 258, 371, 814, 938, 1530, 2308, 3091, 3119
Hpy166II GTNNAC 9 cut(s) 169, 772, 1591, 1593, 2045, 2473, 2800, 2986, 3195
Hpy8I GTNNAC 9 cut(s) 169, 772, 1591, 1593, 2045, 2473, 2800, 2986, 3195
HpyCH4IV ACGT 2 cut(s) 789, 2057
HpySE526I ACGT 2 cut(s) 789, 2057
HspAI GCGC 2 cut(s) 31, 2722
KroI GCCGGC 1 cut(s) 3090
KroNI GCCGGC 1 cut(s) 3092
Ksp22I TGATCA 2 cut(s) 75, 1782
LmnI GCTCC 4 cut(s) 331, 362, 1260, 2679
Lsp1109I GCAGC 3 cut(s) 213, 1038, 2814
LweI GCATC 5 cut(s) 1329, 1755, 2330, 2875, 3105
MaeII ACGT 2 cut(s) 789, 2057
MboII GAAGA 8 cut(s) 42, 140, 261, 520, 1561, 2328, 2524, 3170
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 3 cut(s) 622, 1123, 1526
MhlI GDGCHC 3 cut(s) 632, 2909, 3197
MlyI GAGTC 7 cut(s) 391, 466, 898, 2078, 2111, 2235, 3006
MmeI TCCRAC 4 cut(s) 640, 1101, 1443, 1505
MroNI GCCGGC 1 cut(s) 3090
MroXI GAANNNNTTC 1 cut(s) 2428
MseI TTAA 8 cut(s) 138, 231, 1319, 1641, 1805, 1854, 1970, 1998
MslI CAYNNNNRTG 4 cut(s) 71, 1913, 2805, 2912
MspA1I CMGCKG 2 cut(s) 83, 3200
MspI CCGG 8 cut(s) 258, 371, 814, 938, 1530, 2308, 3091, 3119
MspR9I CCNGG 6 cut(s) 371, 522, 938, 1476, 1531, 1606
MunI CAATTG 1 cut(s) 461
MvaI CCWGG 3 cut(s) 522, 1476, 1606
NaeI GCCGGC 1 cut(s) 3092
NciI CCSGG 3 cut(s) 371, 938, 1531
NdeI CATATG 3 cut(s) 1447, 1625, 2253
NgoMIV GCCGGC 1 cut(s) 3090
NheI GCTAGC 1 cut(s) 2816
NlaIV GGNNCC 6 cut(s) 194, 1528, 1727, 1746, 2262, 3207
NmeAIII GCCGAG 1 cut(s) 290
NmuCI GTSAC 7 cut(s) 242, 414, 785, 1130, 2081, 2292, 2699
NspI RCATGY 1 cut(s) 2402
OliI CACNNNNGTG 1 cut(s) 2912
PagI TCATGA 2 cut(s) 72, 2632
PaqCI CACCTGC 1 cut(s) 1024
PdiI GCCGGC 1 cut(s) 3092
PdmI GAANNNNTTC 1 cut(s) 2428
PfeI GAWTC 8 cut(s) 101, 663, 832, 862, 1490, 1558, 1921, 3099
PflMI CCANNNNNTGG 4 cut(s) 1238, 1264, 1757, 2948
PfoI TCCNGGA 2 cut(s) 369, 936
PkrI GCNGC 3 cut(s) 203, 1053, 2829
PleI GAGTC 7 cut(s) 390, 465, 897, 2077, 2111, 2234, 3005
PpsI GAGTC 7 cut(s) 390, 465, 897, 2077, 2111, 2234, 3005
PpuMI RGGWCCY 1 cut(s) 1744
PshAI GACNNNNGTC 1 cut(s) 381
PsiI TTATAA 2 cut(s) 2240, 3251
Psp124BI GAGCTC 1 cut(s) 632
Psp1406I AACGTT 1 cut(s) 2057
Psp5II RGGWCCY 1 cut(s) 1744
Psp6I CCWGG 3 cut(s) 520, 1474, 1604
PspEI GGTNACC 1 cut(s) 2081
PspFI CCCAGC 1 cut(s) 2918
PspGI CCWGG 3 cut(s) 520, 1474, 1604
PspN4I GGNNCC 6 cut(s) 194, 1528, 1727, 1746, 2262, 3207
PspPI GGNCC 6 cut(s) 1267, 1364, 1478, 1744, 2261, 3066
PspPPI RGGWCCY 1 cut(s) 1744
PstI CTGCAG 2 cut(s) 1684, 1825
PsuI RGATCY 3 cut(s) 622, 1123, 1526
PvuII CAGCTG 2 cut(s) 83, 3200
RseI CAYNNNNRTG 4 cut(s) 71, 1913, 2805, 2912
SacI GAGCTC 1 cut(s) 632
SaqAI TTAA 8 cut(s) 138, 231, 1319, 1641, 1805, 1854, 1970, 1998
SatI GCNGC 3 cut(s) 202, 1052, 2828
Sau96I GGNCC 6 cut(s) 1267, 1364, 1478, 1744, 2261, 3066
SbfI CCTGCAGG 1 cut(s) 1825
ScaI AGTACT 4 cut(s) 404, 590, 1190, 2205
SchI GAGTC 7 cut(s) 391, 466, 898, 2078, 2111, 2235, 3006
ScrFI CCNGG 6 cut(s) 371, 522, 938, 1476, 1531, 1606
SdaI CCTGCAGG 1 cut(s) 1825
SduI GDGCHC 3 cut(s) 632, 2909, 3197
SfaNI GCATC 5 cut(s) 1329, 1755, 2330, 2875, 3105
SfcI CTRYAG 2 cut(s) 1680, 1821
SinI GGWCC 4 cut(s) 1267, 1364, 1478, 1744
SmiMI CAYNNNNRTG 4 cut(s) 71, 1913, 2805, 2912
SmlI CTYRAG 4 cut(s) 379, 398, 1097, 2131
SmoI CTYRAG 4 cut(s) 379, 398, 1097, 2131
SpeI ACTAGT 1 cut(s) 3142
Sse8387I CCTGCAGG 1 cut(s) 1825
SsiI CCGC 1 cut(s) 2728
SspI AATATT 2 cut(s) 1462, 2652
SstI GAGCTC 1 cut(s) 632
StyD4I CCNGG 6 cut(s) 369, 520, 936, 1474, 1529, 1604
StyI CCWWGG 6 cut(s) 172, 1373, 2077, 2345, 2476, 3268
TaiI ACGT 2 cut(s) 792, 2060
TaqI TCGA 5 cut(s) 14, 1618, 1633, 2374, 2554
TfiI GAWTC 8 cut(s) 101, 663, 832, 862, 1490, 1558, 1921, 3099
Tru1I TTAA 8 cut(s) 138, 231, 1319, 1641, 1805, 1854, 1970, 1998
Tru9I TTAA 8 cut(s) 138, 231, 1319, 1641, 1805, 1854, 1970, 1998
TscAI CASTG 9 cut(s) 42, 277, 577, 649, 1269, 2119, 2514, 2748, 3039
TseFI GTSAC 7 cut(s) 242, 414, 785, 1130, 2081, 2292, 2699
TseI GCWGC 3 cut(s) 201, 1051, 2827
Tsp45I GTSAC 7 cut(s) 242, 414, 785, 1130, 2081, 2292, 2699
TspRI CASTG 9 cut(s) 42, 277, 577, 649, 1269, 2119, 2514, 2748, 3039
Van91I CCANNNNNTGG 4 cut(s) 1238, 1264, 1757, 2948
VneI GTGCAC 1 cut(s) 3193
VpaK11BI GGWCC 4 cut(s) 1267, 1364, 1478, 1744
XbaI TCTAGA 1 cut(s) 926
XceI RCATGY 1 cut(s) 2402
XcmI CCANNNNNNNNNTGG 1 cut(s) 3076
XmaJI CCTAGG 1 cut(s) 1373
XmnI GAANNNNTTC 1 cut(s) 2428
ZrmI AGTACT 4 cut(s) 404, 590, 1190, 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.