MD14G1206100.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004694
Physical Location & Seq
Forward (+)
29337285 .. 29340725
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1206100.v1.1.491

Sequence Viewer

Length: 3213 bp
ATGATCACAGGTGCTTCCATGGTAGCCGGAGATTCTCTGGACACAGACAGAGAAGTTCTGCTGAGCCTCAAAGCATTTCTTCAGCAAACCAACCGCGTCAACCAAGGAATATACTCGCAGTGGAATCAGAATAGCAGCAACCCGTGCGAGTGGCCAGGAATTTCGTGCAACCATGACAGCAAGAGAGTCAGCGGCGTTCAGCTCTCCAACCAACATATCACCGGTGAGATTTTCCTAAACTTCTCTGCACTGACAGCACTTTCGCACCTCGACCTCTCGAAAAACACAATAACCGGAGCTCTCCCGGAGGACTTGAGTCAATGCCACAGCCTCAAACACCTCAACTTGTCACATAACACTATAGACGGTGGGCTGAACTTGAATGGTTTGAATCAGCTGGAGGTTCTTGATTTGACTGTCAATAGATTTTACGGCGATCTGGGGATGAGCTTTCCCGGAGTTTGCAGTAACCTAGTGGTTGCAAATCTCTCAGCCAATAATTTTAACGGAAGGATTGATCACATGTTTGATGAATGCTTCAATTTGCAGTACTTGGATTTGAGCACGAACTATTTTAGTGGTGAGATATGGAGTGGATTCAGTAGGCTTACGGAGTTTTCAGTATCAGAAAACTATTTCAATGGCACTATTTTGCCTTCTGTTTTTGCGAATAGTTGTAGTCTTGTCGATTTGGACTTGTCCGAAAACAGTTTTTCTGGTGCGGTTCCTGCAGAGATTTCGAAATGCCAGAGGCTGGTCAAATTGGATCTATGGGGGAACAAGTTTACGGGGCCAATCCCGTCTGAGATTGGATCAATTTCGAGTCTGCAGGCCTTGTTCTTGGGCAACAACAGCTTTTCAAGAGATATCCCGGAATCCCTTTTGGATTTGAACAGCTTGACATTCTTGGATTTGAGCAGGAACAATTTTAGCGGAGATATACAAGACATTTTCGGGAGACTCAGACAGGTGAAGTTTCTTGGGCTGCACTCAAACTGGTACACAGGTGGAATCTATTCCTCTGGCATTCTCAAGTTACCAAATGTTACGAGGTTAGACGTCAGCTACAACAATTTTACTGGTCCTCTGCCGCTTGTTATTTCTCAGATGCCGAAGTTGGAGTTTTTAGTCCTGGCTTTCAATCAATTCAACGGAACTATACCACCGGAGTACGGAAACATTCCAACCCTCCAAGCCCTGGACCTTTCCTTCAACAGCCTAACGGGAGCAATTCCAAGCACCCTCGGAAACTTGAGCTCTCTCTTGTGGTTGATGCTTGCGAACAATTTCCTGACTGGTCCAATTCCACACGAATTGGGAAATTGTGGTAGTTTGCTGTGGCTTAATCTCGAGAACAACAAGCTCTCCGGGCCAATCCCATCTCAACTGACAAAAATTGGGAGAAATGTTAAGCAAACATTCGACAAAAATAATCAGGACTATGATCAGATCATAGGGGGATCAGTAGAGTGCTTGGCGATGAAGAGATGGATTCCTGCAGATTACCCACCTTTCAGTTTCGTGTATACGATCCTCACTAGGAAGAGTTGTCAAGGCATATGGGACCGGCTACTTAAGGGAAACGGCCTATTTGAAATATGTGCAGCTGGCTCTTCTGTACGGACACAACAAATCTCGGGTTACATTCAACTGAGTGGGAATCAGCTTTCGGGTCAGCTCCCACCAGATATTGGTACATTGCGCAATTTTAGCATGATACATTTGGGTGTCAATAGATTCCAAGGCGAACTCCCTGCAAATATCGGTCAGTTGCCACTTGTAGTGTTCAACATCAGTGCGAACAATTTTTCGGGTCAAATTCCGGAGGAGATTGGGAATATTAAGTGCATGCGGAATCTTGATATGTCATATAACAATTTTTCTGGCACATTTCCTGTGAGCCTAAACGGCTTGACTGATTTAAGTAAGTTCAACATTTCGTACAATCCGCTCATCTCCGGCATAATTCCTTCGAGTGGGCAGTTAGCAACCTTCGAGAAAGAATCCTATCTCGGTGACCCCCTCCTGCAGCTTCCCAAATTCATCGACAACTCCACGTATCCAGCAAAAAAACCGTACGGGAATCCGAAAAAGCCTAAAAAGTTTGCTGCATATGTTTTGGTCTTGGTTTTGCTAATTGTTGTCTTGGTATGTGGAGTTTTGTCACTTGTAGCGTGCTTATCCGGAAAGAGCCCGCCACAGCCCCCAGGATACTTGTTGGAGGACATCAAATACCGGCATGACTTGGCCTCGAGCTCTAGCAGTTCATCCCCGTGGTTGTCGGATACTGTGAAGATCATTCGTTTGGACAAAACGGCTTTTACACATGCTGACATTTTGAACGCTACACGCAATTTTTCGGAAGATAGGATTATAGGGAGGGGAGGGTTTGGGACAGTGTACCAAGGAGTATTGCCGGATGGGAGAGTAGTAGCGGTGAAGAAGCTTCTAAGAGAAGGACTCGAAGGTGAAAGGGAGTTCCGAGCTGAAATGGAGGTTCTTAGTGGGAATGGCTTTGGCTGGCCTCACCCGAACCTTGTAACTCTTCACGGCTGGTGTCTCTATGGATCCGAAAAAATACTAGTCTACGAGTACATGGAAGGCGGGACCTTGGAGGATTTTATAACTGATAGAGTAGGACTGACATGGCGAAGGCGAGTTGATGTGGCAGTTGACGTGGCTCGAGCCTTGGTGTTTCTACACCACGAGTGCTTCCCTGCCATCGTGCACCGCGATGTGAAGGCAAGCAATGTGCTGCTAGACAAGGATGGAAAGGCGAGAGTTACAGATTTCGGGCTGGCTAGAATAGTCGATGCAGGGAATACTCATGTGAGCACAATGGTGGCGGGGACTGTTGGCTATGTCGCGCCCGAATATGGGCAGACATGGCAAGCCACGACGAAAGGAGATGTGTACAGTTACGGAGTGCTGGCAATGGAGTTGGCAACCGGGAGGAGAGCGGTGGATGGAGGAGAGGAGTGCCTTGTGGAATGGGCAAGAAGGGTAATGGGAAATGGACATCAAGGATTCAACCGGTCTGTGATACCGGTTATGCTTATGGGATCGGGGCTGGTTGATGGGGCGGAGGAGATGTGCGAGCTGCTTAAGGTTGGCATTAAGTGCACGGCCGAGGCACCACAGTCAAGGCCAAACATGAAGGAGGTTCTAGCTATGCTACTCAATATATATAATGTCGAAATGTTAAATATATAG

Protein Analysis

1071

Amino Acids

117.55

Weight (kDa)

5.76

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 14 - 57 1.6e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 80 - 121 2.3e-07 Leucine rich repeat
LRR_8 PF13855 251 - 310 6.2e-07 Leucine rich repeat
LRR_8 PF13855 395 - 455 1e-08 Leucine rich repeat
Pkinase PF00069 787 - 1056 5.7e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 789 - 1058 1.6e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2624
AatII GACGTC 1 cut(s) 1062
Acc16I TGCGCA 1 cut(s) 1703
AccB1I GGYRCC 1 cut(s) 3133
AccB7I CCANNNNNTGG 3 cut(s) 754, 1198, 1691
AccBSI CCGCTC 2 cut(s) 1951, 2960
AccI GTMKAC 2 cut(s) 1526, 2586
AccII CGCG 3 cut(s) 96, 2733, 2867
AccIII TCCGGA 2 cut(s) 1822, 2183
AclWI GGATC 7 cut(s) 774, 820, 1468, 1525, 2562, 2575, 3070
AcoI YGGCCR 2 cut(s) 152, 3126
AcsI RAATTY 3 cut(s) 159, 1818, 2039
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 1059
AfiI CCNNNNNNNGG 7 cut(s) 754, 1172, 1198, 1691, 1976, 2876, 2877
AflII CTTAAG 2 cut(s) 1574, 3104
AflIII ACRYGT 1 cut(s) 522
AgeI ACCGGT 3 cut(s) 221, 3033, 3046
AhlI ACTAGT 1 cut(s) 2581
AjiI CACGTC 1 cut(s) 2677
AjnI CCWGG 4 cut(s) 154, 1131, 1197, 2206
AleI CACNNNNGTG 1 cut(s) 2840
Alw21I GWGCWC 7 cut(s) 301, 566, 1259, 2258, 2730, 2837, 3125
Alw26I GTCTC 2 cut(s) 952, 2564
Alw44I GTGCAC 2 cut(s) 2726, 3121
AlwI GGATC 7 cut(s) 774, 820, 1468, 1525, 2562, 2575, 3070
AlwNI CAGNNNCTG 1 cut(s) 754
Ama87I CYCGRG 4 cut(s) 1349, 1636, 2251, 2682
Aor13HI TCCGGA 2 cut(s) 1822, 2183
AoxI GGCC 9 cut(s) 152, 791, 831, 1370, 1585, 2247, 2522, 3126, 3146
ApaLI GTGCAC 2 cut(s) 2726, 3121
ApeKI GCWGC 7 cut(s) 135, 985, 1604, 2029, 2108, 2755, 3100
ApoI RAATTY 3 cut(s) 159, 1818, 2039
ArsI GACNNNNNNTTYG 2 cut(s) 244, 276
AsiGI ACCGGT 3 cut(s) 221, 3033, 3046
Asp700I GAANNNNTTC 2 cut(s) 1015, 1286
AspLEI GCGC 2 cut(s) 1704, 2869
AspS9I GGNCC 7 cut(s) 791, 1082, 1201, 1298, 1370, 1564, 2607
AsuC2I CCSGG 5 cut(s) 305, 456, 872, 1369, 2950
AsuHPI GGTGA 8 cut(s) 211, 236, 593, 982, 2027, 2449, 2480, 2519
AsuII TTCGAA 1 cut(s) 740
AvaI CYCGRG 4 cut(s) 1349, 1636, 2251, 2682
AvaII GGWCC 5 cut(s) 1082, 1201, 1298, 1564, 2607
BaeGI GKGCMC 2 cut(s) 2730, 3125
BaeI ACNNNNGTAYC 2 cut(s) 991, 1024
BalI TGGCCA 1 cut(s) 154
BamHI GGATCC 1 cut(s) 2567
BanI GGYRCC 1 cut(s) 3133
BanII GRGCYC 4 cut(s) 301, 1259, 2195, 2258
BauI CACGAG 1 cut(s) 2705
Bbv12I GWGCWC 7 cut(s) 301, 566, 1259, 2258, 2730, 2837, 3125
BbvI GCAGC 7 cut(s) 147, 972, 1616, 2041, 2095, 2742, 3087
BccI CCATC 7 cut(s) 1387, 1482, 2414, 2729, 2762, 2960, 3071
BceAI ACGGC 6 cut(s) 448, 1600, 1924, 2331, 2566, 3141
BcgI CGANNNNNNTGC 4 cut(s) 720, 754, 1736, 1770
BciT130I CCWGG 4 cut(s) 156, 1133, 1199, 2208
BciVI GTATCC 3 cut(s) 2070, 2204, 2278
BclI TGATCA 3 cut(s) 3, 517, 1444
BcnI CCSGG 5 cut(s) 305, 456, 872, 1369, 2950
BcoDI GTCTC 2 cut(s) 952, 2564
BcuI ACTAGT 1 cut(s) 2581
BfaI CTAG 7 cut(s) 473, 1539, 2259, 2582, 2759, 2802, 3167
BfmI CTRYAG 5 cut(s) 360, 729, 827, 1497, 2027
BfrI CTTAAG 2 cut(s) 1574, 3104
BfuI GTATCC 3 cut(s) 2070, 2204, 2278
BglI GCCNNNNNGGC 1 cut(s) 1908
BisI GCNGC 9 cut(s) 136, 193, 986, 1091, 1605, 2030, 2109, 2756, 3101
BlpI GCTNAGC 1 cut(s) 62
BlsI GCNGC 9 cut(s) 137, 194, 987, 1092, 1606, 2031, 2110, 2757, 3102
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 9 cut(s) 156, 305, 456, 872, 1133, 1199, 1369, 2208, 2950
Bme18I GGWCC 5 cut(s) 1082, 1201, 1298, 1564, 2607
BmeT110I CYCGRG 4 cut(s) 1349, 1636, 2251, 2682
BmgBI CACGTC 1 cut(s) 2677
BmgT120I GGNCC 7 cut(s) 791, 1082, 1201, 1298, 1370, 1564, 2607
BmiI GGNNCC 6 cut(s) 726, 792, 1565, 2569, 2608, 3135
BmrFI CCNGG 9 cut(s) 156, 305, 456, 872, 1133, 1199, 1369, 2208, 2950
BmsI GCATC 3 cut(s) 1098, 1263, 2803
BoxI GACNNNNGTC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 2445, 2477
BpmI CTGGAG 1 cut(s) 419
Bpu1102I GCTNAGC 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 740
BpuEI CTTGAG 3 cut(s) 334, 1016, 1273
BpuMI CCSGG 5 cut(s) 305, 456, 872, 1369, 2950
BsaAI YACGTR 1 cut(s) 2058
BsaHI GRCGYC 1 cut(s) 1059
BsaWI WCCGGW 7 cut(s) 221, 293, 1165, 1822, 2183, 3033, 3046
BsaXI ACNNNNNCTCC 4 cut(s) 1349, 1379, 3077, 3107
Bsc4I CCNNNNNNNGG 7 cut(s) 754, 1172, 1198, 1691, 1976, 2876, 2877
Bse118I RCCGGY 5 cut(s) 221, 1566, 2235, 3033, 3046
Bse1I ACTGG 3 cut(s) 1001, 1084, 1300
Bse3DI GCAATG 3 cut(s) 1697, 2755, 2940
BseAI TCCGGA 2 cut(s) 1822, 2183
BseBI CCWGG 4 cut(s) 156, 1133, 1199, 2208
BseGI GGATG 5 cut(s) 450, 2267, 2425, 2773, 2971
BseLI CCNNNNNNNGG 7 cut(s) 754, 1172, 1198, 1691, 1976, 2876, 2877
BseMI GCAATG 3 cut(s) 1697, 2755, 2940
BseMII CTCAG 6 cut(s) 53, 504, 795, 976, 1118, 1643
BseNI ACTGG 3 cut(s) 1001, 1084, 1300
BseRI GAGGAG 5 cut(s) 1841, 2968, 2985, 2990, 3101
BseSI GKGCMC 2 cut(s) 2730, 3125
BseX3I CGGCCG 1 cut(s) 3126
BseXI GCAGC 7 cut(s) 147, 972, 1616, 2041, 2095, 2742, 3087
BsgI GTGCAG 3 cut(s) 231, 971, 1623
Bsh1236I CGCG 3 cut(s) 96, 2733, 2867
Bsh1285I CGRYCG 1 cut(s) 3129
BshFI GGCC 9 cut(s) 154, 793, 833, 1372, 1587, 2249, 2524, 3128, 3148
BshNI GGYRCC 1 cut(s) 3133
BshTI ACCGGT 3 cut(s) 221, 3033, 3046
BsiEI CGRYCG 1 cut(s) 3129
BsiHKAI GWGCWC 7 cut(s) 301, 566, 1259, 2258, 2730, 2837, 3125
BsiHKCI CYCGRG 4 cut(s) 1349, 1636, 2251, 2682
BsiWI CGTACG 1 cut(s) 2076
BslFI GGGAC 4 cut(s) 1577, 2407, 2620, 2863
BslI CCNNNNNNNGG 7 cut(s) 754, 1172, 1198, 1691, 1976, 2876, 2877
BsmAI GTCTC 2 cut(s) 952, 2564
BsmFI GGGAC 4 cut(s) 1577, 2407, 2620, 2863
BsmI GAATGC 2 cut(s) 539, 1026
BsnI GGCC 9 cut(s) 154, 793, 833, 1372, 1587, 2249, 2524, 3128, 3148
BsoBI CYCGRG 4 cut(s) 1349, 1636, 2251, 2682
Bsp119I TTCGAA 1 cut(s) 740
Bsp1286I GDGCHC 8 cut(s) 301, 566, 1259, 2195, 2258, 2730, 2837, 3125
Bsp13I TCCGGA 2 cut(s) 1822, 2183
Bsp1407I TGTACA 1 cut(s) 2913
Bsp1720I GCTNAGC 1 cut(s) 62
Bsp19I CCATGG 1 cut(s) 18
BspANI GGCC 9 cut(s) 154, 793, 833, 1372, 1587, 2249, 2524, 3128, 3148
BspCNI CTCAG 6 cut(s) 54, 503, 796, 975, 1117, 1644
BspEI TCCGGA 2 cut(s) 1822, 2183
BspFNI CGCG 3 cut(s) 96, 2733, 2867
BspLI GGNNCC 6 cut(s) 726, 792, 1565, 2569, 2608, 3135
BspMAI CTGCAG 4 cut(s) 733, 831, 1501, 2031
BspPI GGATC 7 cut(s) 774, 820, 1468, 1525, 2562, 2575, 3070
BspQI GCTCTTC 1 cut(s) 1618
BspT104I TTCGAA 1 cut(s) 740
BspT107I GGYRCC 1 cut(s) 3133
BspTI CTTAAG 2 cut(s) 1574, 3104
BsrBI CCGCTC 2 cut(s) 1951, 2960
BsrDI GCAATG 3 cut(s) 1697, 2755, 2940
BsrFI RCCGGY 5 cut(s) 221, 1566, 2235, 3033, 3046
BsrGI TGTACA 1 cut(s) 2913
BsrI ACTGG 3 cut(s) 1001, 1084, 1300
BssAI RCCGGY 5 cut(s) 221, 1566, 2235, 3033, 3046
BssNAI GTATAC 1 cut(s) 1527
BssNI GRCGYC 1 cut(s) 1059
BssSI CACGAG 1 cut(s) 2705
BssT1I CCWWGG 6 cut(s) 18, 103, 1741, 2404, 2610, 2688
Bst1107I GTATAC 1 cut(s) 1527
Bst2BI CACGAG 1 cut(s) 2705
Bst2UI CCWGG 4 cut(s) 156, 1133, 1199, 2208
Bst4CI ACNGT 9 cut(s) 368, 418, 710, 2076, 2290, 2398, 2854, 2918, 3141
Bst6I CTCTTC 4 cut(s) 1478, 1538, 1618, 2550
BstACI GRCGYC 1 cut(s) 1059
BstAFI CTTAAG 2 cut(s) 1574, 3104
BstAPI GCANNNNNTGC 2 cut(s) 144, 3120
BstAUI TGTACA 1 cut(s) 2913
BstBAI YACGTR 1 cut(s) 2058
BstBI TTCGAA 1 cut(s) 740
BstDEI CTNAG 8 cut(s) 62, 490, 804, 962, 1104, 1652, 2450, 2501
BstDSI CCRYGG 2 cut(s) 18, 2273
BstEII GGTNACC 1 cut(s) 2015
BstF5I GGATG 5 cut(s) 450, 2267, 2425, 2773, 2971
BstFNI CGCG 3 cut(s) 96, 2733, 2867
BstHHI GCGC 2 cut(s) 1704, 2869
BstMAI GTCTC 2 cut(s) 952, 2564
BstMCI CGRYCG 1 cut(s) 3129
BstMWI GCNNNNNNNGC 9 cut(s) 144, 254, 728, 852, 1369, 1710, 1908, 2887, 3120
BstNI CCWGG 4 cut(s) 156, 1133, 1199, 2208
BstNSI RCATGY 3 cut(s) 526, 1852, 2330
BstPAI GACNNNNGTC 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 2015
BstSCI CCNGG 9 cut(s) 154, 303, 454, 870, 1131, 1197, 1367, 2206, 2948
BstSFI CTRYAG 5 cut(s) 360, 729, 827, 1497, 2027
BstSLI GKGCMC 2 cut(s) 2730, 3125
BstUI CGCG 3 cut(s) 96, 2733, 2867
BstV1I GCAGC 7 cut(s) 147, 972, 1616, 2041, 2095, 2742, 3087
BstX2I RGATCY 2 cut(s) 766, 2567
BstYI RGATCY 2 cut(s) 766, 2567
BstZ17I GTATAC 1 cut(s) 1527
BstZI CGGCCG 1 cut(s) 3126
BsuI GTATCC 3 cut(s) 2070, 2204, 2278
BsuRI GGCC 9 cut(s) 154, 793, 833, 1372, 1587, 2249, 2524, 3128, 3148
BtgI CCRYGG 2 cut(s) 18, 2273
BtgZI GCGATG 2 cut(s) 1493, 2748
BtrI CACGTC 1 cut(s) 2677
BtsCI GGATG 5 cut(s) 450, 2267, 2425, 2773, 2971
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 4 cut(s) 125, 248, 1801, 2403
CaiI CAGNNNCTG 1 cut(s) 754
CfoI GCGC 2 cut(s) 1704, 2869
Cfr10I RCCGGY 5 cut(s) 221, 1566, 2235, 3033, 3046
Cfr13I GGNCC 7 cut(s) 791, 1082, 1201, 1298, 1370, 1564, 2607
CseI GACGC 1 cut(s) 85
CspAI ACCGGT 3 cut(s) 221, 3033, 3046
CspCI CAANNNNNGTGG 2 cut(s) 1296, 1331
DdeI CTNAG 8 cut(s) 62, 490, 804, 962, 1104, 1652, 2450, 2501
EaeI YGGCCR 2 cut(s) 152, 3126
EagI CGGCCG 1 cut(s) 3126
Eam1104I CTCTTC 4 cut(s) 1478, 1538, 1618, 2550
EarI CTCTTC 4 cut(s) 1478, 1538, 1618, 2550
EciI GGCGGA 1 cut(s) 3098
Ecl136II GAGCTC 3 cut(s) 299, 1257, 2256
EclXI CGGCCG 1 cut(s) 3126
Eco130I CCWWGG 6 cut(s) 18, 103, 1741, 2404, 2610, 2688
Eco147I AGGCCT 1 cut(s) 833
Eco24I GRGCYC 4 cut(s) 301, 1259, 2195, 2258
Eco32I GATATC 1 cut(s) 868
Eco47I GGWCC 5 cut(s) 1082, 1201, 1298, 1564, 2607
Eco52I CGGCCG 1 cut(s) 3126
Eco53kI GAGCTC 3 cut(s) 299, 1257, 2256
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 4 cut(s) 1349, 1636, 2251, 2682
Eco91I GGTNACC 1 cut(s) 2015
EcoICRI GAGCTC 3 cut(s) 299, 1257, 2256
EcoO109I RGGNCCY 1 cut(s) 2607
EcoO65I GGTNACC 1 cut(s) 2015
EcoRII CCWGG 4 cut(s) 154, 1131, 1197, 2206
EcoRV GATATC 1 cut(s) 868
EcoT14I CCWWGG 6 cut(s) 18, 103, 1741, 2404, 2610, 2688
EcoT38I GRGCYC 4 cut(s) 301, 1259, 2195, 2258
ErhI CCWWGG 6 cut(s) 18, 103, 1741, 2404, 2610, 2688
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FaqI GGGAC 4 cut(s) 1577, 2407, 2620, 2863
FauI CCCGC 3 cut(s) 2202, 2597, 2839
FauNDI CATATG 2 cut(s) 1559, 2113
FbaI TGATCA 3 cut(s) 3, 517, 1444
FblI GTMKAC 2 cut(s) 1526, 2586
Fnu4HI GCNGC 9 cut(s) 136, 193, 986, 1091, 1605, 2030, 2109, 2756, 3101
FokI GGATG 5 cut(s) 457, 2254, 2432, 2780, 2978
FriOI GRGCYC 4 cut(s) 301, 1259, 2195, 2258
Fsp4HI GCNGC 9 cut(s) 136, 193, 986, 1091, 1605, 2030, 2109, 2756, 3101
FspBI CTAG 7 cut(s) 473, 1539, 2259, 2582, 2759, 2802, 3167
FspI TGCGCA 1 cut(s) 1703
GlaI GCGC 2 cut(s) 1703, 2868
GluI GCNGC 9 cut(s) 136, 193, 986, 1091, 1605, 2030, 2109, 2756, 3101
GsuI CTGGAG 1 cut(s) 419
HaeIII GGCC 9 cut(s) 154, 793, 833, 1372, 1587, 2249, 2524, 3128, 3148
HgaI GACGC 1 cut(s) 85
HhaI GCGC 2 cut(s) 1704, 2869
Hin1I GRCGYC 1 cut(s) 1059
Hin6I GCGC 2 cut(s) 1702, 2867
HinP1I GCGC 2 cut(s) 1702, 2867
HincII GTYRAC 2 cut(s) 100, 2674
HindII GTYRAC 2 cut(s) 100, 2674
HindIII AAGCTT 1 cut(s) 2444
HphI GGTGA 8 cut(s) 211, 236, 593, 982, 2027, 2449, 2480, 2519
Hpy99I CGWCG 1 cut(s) 2902
HpyCH4III ACNGT 9 cut(s) 368, 418, 710, 2076, 2290, 2398, 2854, 2918, 3141
HpyCH4IV ACGT 3 cut(s) 1059, 2057, 2676
HpyF10VI GCNNNNNNNGC 9 cut(s) 144, 254, 728, 852, 1369, 1710, 1908, 2887, 3120
HpyF3I CTNAG 8 cut(s) 62, 490, 804, 962, 1104, 1652, 2450, 2501
HpySE526I ACGT 3 cut(s) 1059, 2057, 2676
Hsp92I GRCGYC 1 cut(s) 1059
HspAI GCGC 2 cut(s) 1702, 2867
Kpn2I TCCGGA 2 cut(s) 1822, 2183
Ksp22I TGATCA 3 cut(s) 3, 517, 1444
LguI GCTCTTC 1 cut(s) 1618
LmnI GCTCC 3 cut(s) 296, 1226, 1683
Lsp1109I GCAGC 7 cut(s) 147, 972, 1616, 2041, 2095, 2742, 3087
LweI GCATC 3 cut(s) 1098, 1263, 2803
MaeI CTAG 7 cut(s) 473, 1539, 2259, 2582, 2759, 2802, 3167
MaeII ACGT 3 cut(s) 1059, 2057, 2676
MbiI CCGCTC 2 cut(s) 1951, 2960
MboII GAAGA 8 cut(s) 71, 1495, 1555, 1605, 2305, 2375, 2452, 2537
MflI RGATCY 2 cut(s) 766, 2567
MhlI GDGCHC 8 cut(s) 301, 566, 1259, 2195, 2258, 2730, 2837, 3125
MlsI TGGCCA 1 cut(s) 154
MluNI TGGCCA 1 cut(s) 154
MlyI GAGTC 5 cut(s) 195, 325, 832, 954, 2454
MmeI TCCRAC 5 cut(s) 231, 1098, 1208, 2199, 2262
Mox20I TGGCCA 1 cut(s) 154
MroI TCCGGA 2 cut(s) 1822, 2183
MroXI GAANNNNTTC 2 cut(s) 1015, 1286
MscI TGGCCA 1 cut(s) 154
MseI TTAA 9 cut(s) 504, 1344, 1410, 1575, 1842, 1922, 3105, 3117, 3203
MslI CAYNNNNRTG 4 cut(s) 1725, 2272, 2733, 2840
Msp20I TGGCCA 1 cut(s) 154
MspA1I CMGCKG 3 cut(s) 192, 397, 1607
MspCI CTTAAG 2 cut(s) 1574, 3104
MspR9I CCNGG 9 cut(s) 156, 305, 456, 872, 1133, 1199, 1369, 2208, 2950
Mva1269I GAATGC 2 cut(s) 539, 1026
MvaI CCWGG 4 cut(s) 156, 1133, 1199, 2208
MvnI CGCG 3 cut(s) 96, 2733, 2867
MwoI GCNNNNNNNGC 9 cut(s) 144, 254, 728, 852, 1369, 1710, 1908, 2887, 3120
NciI CCSGG 5 cut(s) 305, 456, 872, 1369, 2950
NcoI CCATGG 1 cut(s) 18
NdeI CATATG 2 cut(s) 1559, 2113
NlaIV GGNNCC 6 cut(s) 726, 792, 1565, 2569, 2608, 3135
NmeAIII GCCGAG 1 cut(s) 3154
NmuCI GTSAC 3 cut(s) 348, 2015, 2163
NsbI TGCGCA 1 cut(s) 1703
NspI RCATGY 3 cut(s) 526, 1852, 2330
NspV TTCGAA 1 cut(s) 740
OliI CACNNNNGTG 1 cut(s) 2840
PaeI GCATGC 1 cut(s) 1852
PaeR7I CTCGAG 3 cut(s) 1349, 2251, 2682
PceI AGGCCT 1 cut(s) 833
PciI ACATGT 1 cut(s) 522
PciSI GCTCTTC 1 cut(s) 1618
PcsI WCGNNNNNNNCGW 1 cut(s) 2730
PctI GAATGC 2 cut(s) 539, 1026
PdmI GAANNNNTTC 2 cut(s) 1015, 1286
Pfl23II CGTACG 1 cut(s) 2076
PflMI CCANNNNNTGG 3 cut(s) 754, 1198, 1691
PfoI TCCNGGA 3 cut(s) 303, 454, 870
PinAI ACCGGT 3 cut(s) 221, 3033, 3046
PkrI GCNGC 9 cut(s) 137, 194, 987, 1092, 1606, 2031, 2110, 2757, 3102
PleI GAGTC 5 cut(s) 194, 324, 831, 954, 2454
PpsI GAGTC 5 cut(s) 194, 324, 831, 954, 2454
Ppu21I YACGTR 1 cut(s) 2058
PpuMI RGGWCCY 1 cut(s) 2607
PscI ACATGT 1 cut(s) 522
PshAI GACNNNNGTC 1 cut(s) 315
PsiI TTATAA 1 cut(s) 2624
Psp124BI GAGCTC 3 cut(s) 301, 1259, 2258
Psp5II RGGWCCY 1 cut(s) 2607
Psp6I CCWGG 4 cut(s) 154, 1131, 1197, 2206
PspEI GGTNACC 1 cut(s) 2015
PspGI CCWGG 4 cut(s) 154, 1131, 1197, 2206
PspLI CGTACG 1 cut(s) 2076
PspN4I GGNNCC 6 cut(s) 726, 792, 1565, 2569, 2608, 3135
PspPI GGNCC 7 cut(s) 791, 1082, 1201, 1298, 1370, 1564, 2607
PspPPI RGGWCCY 1 cut(s) 2607
PspXI VCTCGAGB 2 cut(s) 2251, 2682
PstI CTGCAG 4 cut(s) 733, 831, 1501, 2031
PstNI CAGNNNCTG 1 cut(s) 754
PsuI RGATCY 2 cut(s) 766, 2567
PvuII CAGCTG 2 cut(s) 397, 1607
RseI CAYNNNNRTG 4 cut(s) 1725, 2272, 2733, 2840
SacI GAGCTC 3 cut(s) 301, 1259, 2258
SapI GCTCTTC 1 cut(s) 1618
SaqAI TTAA 9 cut(s) 504, 1344, 1410, 1575, 1842, 1922, 3105, 3117, 3203
SatI GCNGC 9 cut(s) 136, 193, 986, 1091, 1605, 2030, 2109, 2756, 3101
Sau96I GGNCC 7 cut(s) 791, 1082, 1201, 1298, 1370, 1564, 2607
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 5 cut(s) 195, 325, 832, 954, 2454
ScrFI CCNGG 9 cut(s) 156, 305, 456, 872, 1133, 1199, 1369, 2208, 2950
SduI GDGCHC 8 cut(s) 301, 566, 1259, 2195, 2258, 2730, 2837, 3125
SfaNI GCATC 3 cut(s) 1098, 1263, 2803
SfcI CTRYAG 5 cut(s) 360, 729, 827, 1497, 2027
Sfr274I CTCGAG 3 cut(s) 1349, 2251, 2682
SfuI TTCGAA 1 cut(s) 740
SgrAI CRCCGGYG 1 cut(s) 221
SinI GGWCC 5 cut(s) 1082, 1201, 1298, 1564, 2607
SlaI CTCGAG 3 cut(s) 1349, 2251, 2682
SmiMI CAYNNNNRTG 4 cut(s) 1725, 2272, 2733, 2840
SmlI CTYRAG 8 cut(s) 313, 1031, 1252, 1349, 1574, 2251, 2682, 3104
SmoI CTYRAG 8 cut(s) 313, 1031, 1252, 1349, 1574, 2251, 2682, 3104
SpeI ACTAGT 1 cut(s) 2581
SphI GCATGC 1 cut(s) 1852
SseBI AGGCCT 1 cut(s) 833
SspI AATATT 1 cut(s) 1840
SspMI CTAG 7 cut(s) 473, 1539, 2259, 2582, 2759, 2802, 3167
SstI GAGCTC 3 cut(s) 301, 1259, 2258
StuI AGGCCT 1 cut(s) 833
StyD4I CCNGG 9 cut(s) 154, 303, 454, 870, 1131, 1197, 1367, 2206, 2948
StyI CCWWGG 6 cut(s) 18, 103, 1741, 2404, 2610, 2688
TaaI ACNGT 9 cut(s) 368, 418, 710, 2076, 2290, 2398, 2854, 2918, 3141
TaiI ACGT 3 cut(s) 1062, 2060, 2679
TaqII GACCGA 1 cut(s) 1754
TatI WGTACW 3 cut(s) 549, 2592, 2913
TauI GCSGC 2 cut(s) 195, 1093
Tru1I TTAA 9 cut(s) 504, 1344, 1410, 1575, 1842, 1922, 3105, 3117, 3203
Tru9I TTAA 9 cut(s) 504, 1344, 1410, 1575, 1842, 1922, 3105, 3117, 3203
TscAI CASTG 4 cut(s) 125, 255, 1801, 2403
TseFI GTSAC 3 cut(s) 348, 2015, 2163
TseI GCWGC 7 cut(s) 135, 985, 1604, 2029, 2108, 2755, 3100
Tsp45I GTSAC 3 cut(s) 348, 2015, 2163
TspDTI ATGAA 5 cut(s) 546, 1496, 2032, 2256, 3170
TspGWI ACGGA 6 cut(s) 522, 626, 1167, 1188, 1636, 2937
TspRI CASTG 4 cut(s) 125, 255, 1801, 2403
Van91I CCANNNNNTGG 3 cut(s) 754, 1198, 1691
Vha464I CTTAAG 2 cut(s) 1574, 3104
VneI GTGCAC 2 cut(s) 2726, 3121
VpaK11BI GGWCC 5 cut(s) 1082, 1201, 1298, 1564, 2607
XapI RAATTY 3 cut(s) 159, 1818, 2039
XceI RCATGY 3 cut(s) 526, 1852, 2330
XhoI CTCGAG 3 cut(s) 1349, 2251, 2682
XmiI GTMKAC 2 cut(s) 1526, 2586
XmnI GAANNNNTTC 2 cut(s) 1015, 1286
XspI CTAG 7 cut(s) 473, 1539, 2259, 2582, 2759, 2802, 3167
ZraI GACGTC 1 cut(s) 1060
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.