Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0004694

Physical Location & Seq

Genomic Coordinates Forward (+)

21494915 .. 21498455

Gene Structure 3541 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g17560.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3294 bp

ATGTACTTATCAACTTCGAAAACTGGTTCATGGCGTGCGGTGTGTTCAATATTCTTGGTCTTAGACGTGATCATGATCACAGGTGCTTCCGTTGTAGTCGGAGATTCTCTGGACACAGACAGAGAAGTTCTGCTGAGCCTCAAAGCATTTCTGCAGCAAAAGAACCGCGTAAACCAAGGAATATACTCGCAGTGGAACCAGCGCAGCAGCAACCCCTGCGAGTGGTCCGGAATTTCATGCAGCAATGACAGCAAGAGAGTTAGCCGTGTTGAGCTCAGCGACCAAAAAATCACCGGTGAGATTTTCCGAAACTTCTCTGCCCTTACAGCACTTTCGTACCTCGACCTCTCGAAGAACACAATAAGCGGAGCACTCCCGGAGGACTTGAGTCAATGCCACAGCCTCAGACACCTCAATCTGTCACATAACATTATTGATGGCCAGCTGAACTTGAATGGTTTGAATAAGTTGGAGGTTCTTGATTTGACTGTCAATAGATTTTACGGCAATTTGAAGATGACCTTTCCCGGAGTTTGCAGTAACCTAGTGGTTGCGAATCTTTCAGCGAATAATTTCACCGGAAGGATTGATAGCCTGTTTGATGAATGCTTTAATTTGCAGCACTTGGATTTGAGCTCAAACTATCTTAGTGGTGAGATATGGAGTGGATTCATCAGGCTTACGGCGTTTTCAGTATCCGAAAACTATTTCAGTGGCGCTATTTCGCCTTCTGTTTTCGCAAATAATTGTAGCCTGGTAAAGTTGGACTTGTCAGTAAACAGGTTTTCTGGTGCAGTTCCTGCAGAGATTTCGAAATGCCAGAGACTGATCATATTGAATCTTTGGGGGAACCATTTTACGGGGCCAATCCCGCCTGAGATTGGATCAATTTCGGGACTGCAGGCCTTGTTCTTGGGTAACAACAGTTTTTCTAGAGATATTCCTGAATCCCTTTTGGATTTGACCAGCATGACATTCTTGGATTTGAGCAGGAACGACTTTGGCGGCAAGATACAAGACATTTTTGGGAGATTCAGACAGGTGAAGTTTCTTTTGCTGCACTCAAACGGGTACACCGGTGGTATCCATTCGTCTGGGATTCTCAAGCTACCAAATGTTTCCAGGTTAGACCTCAGCTACAACAATTTTACAGGTCCTCTGCCAGTCGAAATTTCTCTGCCGAAGTTGAAGTTATTTGTCCTCGCCTTTAATCAATTCAACGGAACTATACCACCAGACTATGGAAACATTTCGAGCCTCCAAGCCCTGGACCTTTCCTTCAACAGCCTAACCGGTGCAATTCCTAGCACCCTCGGAAACTTGAGATCTCTCTTGTGGTTGATGCTTGCGAACAATTCCCTAACCGGTCCAATTCCACACGAATTGGGAAATTGTAGTAGCTTGCTGTGGCTTAATCTCGCGAATAACAAGCTCTCCGGGCCAATCCCATCTCAGCTAACAATGATTGGGAGAAATGCTAAGCCAACATTCGACATGAATAATCGGGACAATGATCAGATCGTAGCGGGATCAAGCGAATGCTTAGCGATGAGGAGATGGATTCCTGCAGATTACCCACCATTCAATTTCGTGTACACGATCCTCACTAGGAAGAGTTGTCGAAGCATATGGGACCGGCTGCTTAAAGGAAACGGCCTATTTCCCATATGTGTAGCTGGCTCTTCTGTACGGACGCTTCAAATCTCGGGTTACATTCAACTGAGTGGGAATCAGATTTCGGGTCAGCTCCCTGCAGATATTGGTACAATGCACAGTTTTAGCATGATAAATTTAGGTGTCAATAGATTCCAAGGCGAACTCCCTGCAAATATTGGTCAGTTGCCACTTGTAGTGTTCAACATCAGCAGGAACAATTTTTCGGGTCAAATTCCGGAGGAGATTGGGAATATTAAGTGCATGCAGAATCTTGATATGTCATATAACAATTTTTCTGGCACATTTCCTGTGAGCCTGAACGACTTGAATGAGCTAAGTAATTTCAACATTTCGTACAATCCGCTCATTTCCGGCACAATTCCTTCGACCGGGCAGTTGGCAACGTTCGAGAAAGCGTCCTATCTCGGTAACCCCCTCCTGCAGCTTCCCAAATTCATCGACAACTCCACGTATCCAGAAAACAAAATGAATGGGAATCCAACAAAGCCTACAAAATCTGCTGCATATATGGTGGTCTTCGGTTTGCTAATTGCTCTCTTAATCTGTGGAGTTTTGTCACTTGTAGCATGGTTATTCAGAAAGAGCCGGCCGCAGCCCCCAGGATGTTTGTTGCAGGACATCAAATACCGGCATGACTTGGCCTCGAGCTCTAGCAGTTCATCCCCGTGGCTGTCAGATATTGTTAAGATCATTCGTTTGGACAAAATGGCTTTTACACATGCTGACATTTTGAAAGCTACACGCAATTTTTCGGAAGATAGGATTATAGGGAGTGGAGGGTTTGGGACAGTGTACCAAGGAGTACTGCCGGATGGGAGAGTAGTAGCGGTGAAGAAGCTTCAAAGAGAAGGGCTCGAAGGTGAAAGGGAGTTCCAAGCTGAAATGGAGGTTCTTAGTGGGAATGGCATTGGCTGGCCTCACCCGAACCTTGTAACTCTTCATGGCTGGTGCCTTTATGGATCCGAAAAAATACTAGTCTACGAGTACATGGAAGGCGGGACCTTGGAGGATTTGATATCTGATAGAGTGAGACTGACATGGCGAAGGCGAGTTGATGTGGCAGTTGATGTGGCTCGAGCCTTGATGTTTCTACACCACGAGTGCTTCCCTGCCATCGTGCACCGCGATGTGAAGGCGAGCAATGTGCTGCTAGATAAGGATGGAAAGGCGAGAGTTACAGATTTCGGGCTTGCTAGAATAGTCGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCGGGGACTGTTGGTTATGTCGCTCCCGAATATGGGCAGACATGGCAAGCCACGACGAAAGGAGATGTGTACAGCTACGGAGTTCTGGCAATGGAATTGGCGACCGGAAGGAGAGCGGTGGATGGAGGAGAGGAGTGCCTTGTGGAATGGGCAAGGAGGGTAATGGGAAATGGACGGCAAGGATTCAACCAGTCTGTGATACCGGTTATGCTCATGGGGTCCGGGCTGGCCGATGGGGCGGAGGAGATGTGCGAGCTGCTTAAGGTGGGCATAAAGTGCACGGCGGAGGCACCGCAGTCAAGGCCAAACATGAAGGAGGTTCTAGCTATGCTACTCAAGATATATAAAGATGAAAACATATATAGTTTACAAAGTTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009791 GO:0009825 GO:0009845 GO:0009987 GO:0016020 GO:0016049 GO:0032501 GO:0032502 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0050896 GO:0071944 GO:0090351

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1098

Amino Acids

120.78

Weight (kDa)

6.98

Isoelectric Point (pI)

37.56

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004694)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74360
fragaria_vesca	FvH4_4g04300 FvH4_4g04300 FvH4_5g14410
malus_domestica	MD06G1198600.v1.1 MD14G1206100.v1.1
prunus_persica	Prupe.5G199400_v2.0.a1 Prupe.5G199400_v2.0.a1
pyrus_communis	pycom06g17560
rosa_chinensis	RchiOBHm_Chr4g0394751 RchiOBHm_Chr7g0182251
rosa_laevigata	RLG00000005136 RLG00000009666
rosa_multiflora	Rmu_co8099376.1_g000001 Rmu_sc0000003.1_g000010 Rmu_sc0005356.1_g000009
rosa_roxburghii	Rroxscaffold_3G00271380 Rroxscaffold_5G00339760 Rroxscaffold_5G00339770 Rroxscaffold_5G00339780
rosa_rugosa	Rorug03G0362000.1 Rorug05G0089900 Rorug06G0446400
rosa_samantha	Rh4BG052600 Rh4CG059400 Rh4DG051200 Rh7AG049500 Rh7BG049600 Rh7CG050900 Rh7DG048800
rosa_wichuraiana	Rw7G004060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	2625, 3202
AccB7I	CCANNNNNTGG	2 cut(s)	1241, 1267
AccBSI	CCGCTC	2 cut(s)	2020, 3029
AccI	GTMKAC	1 cut(s)	2655
AccII	CGCG	3 cut(s)	168, 1421, 2802
AccIII	TCCGGA	2 cut(s)	227, 1891
AclI	AACGTT	1 cut(s)	2060
AclWI	GGATC	5 cut(s)	892, 1537, 1594, 2631, 2644
AcoI	YGGCCR	3 cut(s)	439, 2264, 3141
AcsI	RAATTY	5 cut(s)	231, 1170, 1789, 1887, 2108
AfiI	CCNNNNNNNGG	8 cut(s)	222, 859, 881, 1241, 1267, 2045, 2945, 2946
AflII	CTTAAG	1 cut(s)	3173
AgeI	ACCGGT	5 cut(s)	293, 1076, 1292, 1364, 3115
AhlI	ACTAGT	1 cut(s)	2650
AjiI	CACGTC	1 cut(s)	67
AjnI	CCWGG	4 cut(s)	753, 1121, 1266, 2275
AleI	CACNNNNGTG	1 cut(s)	2909
Alw21I	GWGCWC	7 cut(s)	276, 373, 638, 2327, 2799, 2906, 3194
Alw26I	GTCTC	2 cut(s)	817, 2701
Alw44I	GTGCAC	2 cut(s)	2795, 3190
AlwI	GGATC	5 cut(s)	892, 1537, 1594, 2631, 2644
AlwNI	CAGNNNCTG	2 cut(s)	800, 826
Ama87I	CYCGRG	3 cut(s)	1705, 2320, 2751
Aor13HI	TCCGGA	2 cut(s)	227, 1891
ApaLI	GTGCAC	2 cut(s)	2795, 3190
ApoI	RAATTY	5 cut(s)	231, 1170, 1789, 1887, 2108
AsiGI	ACCGGT	5 cut(s)	293, 1076, 1292, 1364, 3115
Asp700I	GAANNNNTTC	2 cut(s)	572, 1249
AspLEI	GCGC	2 cut(s)	204, 719
AspS9I	GGNCC	9 cut(s)	225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
AsuC2I	CCSGG	5 cut(s)	377, 528, 1438, 2047, 3136
AsuHPI	GGTGA	8 cut(s)	283, 308, 568, 665, 1054, 2518, 2549, 2588
AsuII	TTCGAA	2 cut(s)	17, 812
AvaI	CYCGRG	3 cut(s)	1705, 2320, 2751
AvaII	GGWCC	7 cut(s)	225, 1154, 1270, 1367, 1633, 2676, 3132
BaeGI	GKGCMC	2 cut(s)	2799, 3194
BaeI	ACNNNNGTAYC	4 cut(s)	320, 353, 1063, 1096
BalI	TGGCCA	1 cut(s)	441
BamHI	GGATCC	1 cut(s)	2636
BanI	GGYRCC	2 cut(s)	2625, 3202
BanII	GRGCYC	4 cut(s)	276, 638, 2327, 2532
BarI	GAAGNNNNNNTAC	2 cut(s)	1680, 1712
BauI	CACGAG	1 cut(s)	2774
BbsI	GAAGAC	1 cut(s)	2185
Bbv12I	GWGCWC	7 cut(s)	276, 373, 638, 2327, 2799, 2906, 3194
BbvCI	CCTCAGC	1 cut(s)	1133
BccI	CCATC	8 cut(s)	431, 1456, 1551, 2483, 2798, 2831, 3029, 3140
BceAI	ACGGC	6 cut(s)	249, 520, 699, 1669, 3104, 3210
BcgI	CGANNNNNNTGC	6 cut(s)	792, 826, 1805, 1839, 2803, 2837
BciT130I	CCWGG	4 cut(s)	755, 1123, 1268, 2277
BciVI	GTATCC	3 cut(s)	706, 1094, 2139
BclI	TGATCA	4 cut(s)	69, 75, 828, 1513
BcnI	CCSGG	5 cut(s)	377, 528, 1438, 2047, 3136
BcoDI	GTCTC	2 cut(s)	817, 2701
BcuI	ACTAGT	1 cut(s)	2650
BfaI	CTAG	9 cut(s)	545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
BfmI	CTRYAG	6 cut(s)	152, 801, 899, 1566, 1752, 2096
BfoI	RGCGCY	1 cut(s)	720
BfrI	CTTAAG	1 cut(s)	3173
BfuI	GTATCC	3 cut(s)	706, 1094, 2139
BglI	GCCNNNNNGGC	1 cut(s)	3149
BglII	AGATCT	1 cut(s)	1325
BlpI	GCTNAGC	4 cut(s)	134, 275, 1479, 1543
BmcAI	AGTACT	1 cut(s)	2481
Bme1390I	CCNGG	9 cut(s)	377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
Bme18I	GGWCC	7 cut(s)	225, 1154, 1270, 1367, 1633, 2676, 3132
BmeT110I	CYCGRG	3 cut(s)	1705, 2320, 2751
BmgBI	CACGTC	1 cut(s)	67
BmgT120I	GGNCC	9 cut(s)	225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
BmiI	GGNNCC	9 cut(s)	197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
BmrFI	CCNGG	9 cut(s)	377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
BmsI	GCATC	2 cut(s)	1332, 2872
BoxI	GACNNNNGTC	1 cut(s)	387
BpiI	GAAGAC	1 cut(s)	2185
BplI	GAGNNNNNCTC	2 cut(s)	2514, 2546
Bpu10I	CCTNAGC	1 cut(s)	1133
Bpu1102I	GCTNAGC	4 cut(s)	134, 275, 1479, 1543
Bpu14I	TTCGAA	2 cut(s)	17, 812
BpuEI	CTTGAG	4 cut(s)	406, 1088, 1342, 3233
BpuMI	CCSGG	5 cut(s)	377, 528, 1438, 2047, 3136
BsaAI	YACGTR	1 cut(s)	2127
BsaBI	GATNNNNATC	1 cut(s)	74
BsaJI	CCNNGG	8 cut(s)	175, 1266, 1312, 1810, 2275, 2342, 2473, 2679
BsaWI	WCCGGW	9 cut(s)	227, 293, 578, 1076, 1292, 1364, 1891, 3017, 3115
BsaXI	ACNNNNNCTCC	4 cut(s)	1418, 1448, 3146, 3176
Bsc4I	CCNNNNNNNGG	8 cut(s)	222, 859, 881, 1241, 1267, 2045, 2945, 2946
Bse118I	RCCGGY	8 cut(s)	293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
Bse1I	ACTGG	3 cut(s)	28, 1163, 3103
Bse3DI	GCAATG	3 cut(s)	250, 2824, 3009
Bse8I	GATNNNNATC	1 cut(s)	74
BseAI	TCCGGA	2 cut(s)	227, 1891
BseBI	CCWGG	4 cut(s)	755, 1123, 1268, 2277
BseDI	CCNNGG	8 cut(s)	175, 1266, 1312, 1810, 2275, 2342, 2473, 2679
BseGI	GGATG	5 cut(s)	2285, 2336, 2494, 2842, 3040
BseJI	GATNNNNATC	1 cut(s)	74
BseLI	CCNNNNNNNGG	8 cut(s)	222, 859, 881, 1241, 1267, 2045, 2945, 2946
BseMI	GCAATG	3 cut(s)	250, 2824, 3009
BseMII	CTCAG	7 cut(s)	125, 289, 418, 867, 1147, 1466, 1712
BseNI	ACTGG	3 cut(s)	28, 1163, 3103
BseRI	GAGGAG	5 cut(s)	1567, 1910, 3054, 3059, 3170
BseSI	GKGCMC	2 cut(s)	2799, 3194
BseX3I	CGGCCG	1 cut(s)	2264
BsgI	GTGCAG	2 cut(s)	813, 1043
Bsh1236I	CGCG	3 cut(s)	168, 1421, 2802
Bsh1285I	CGRYCG	3 cut(s)	2046, 2267, 3018
BshNI	GGYRCC	2 cut(s)	2625, 3202
BshTI	ACCGGT	5 cut(s)	293, 1076, 1292, 1364, 3115
BsiEI	CGRYCG	3 cut(s)	2046, 2267, 3018
BsiHKAI	GWGCWC	7 cut(s)	276, 373, 638, 2327, 2799, 2906, 3194
BsiHKCI	CYCGRG	3 cut(s)	1705, 2320, 2751
BslFI	GGGAC	6 cut(s)	909, 1520, 1646, 2476, 2689, 2932
BslI	CCNNNNNNNGG	8 cut(s)	222, 859, 881, 1241, 1267, 2045, 2945, 2946
BsmAI	GTCTC	2 cut(s)	817, 2701
BsmFI	GGGAC	6 cut(s)	909, 1520, 1646, 2476, 2689, 2932
BsmI	GAATGC	2 cut(s)	611, 1544
BsoBI	CYCGRG	3 cut(s)	1705, 2320, 2751
Bsp119I	TTCGAA	2 cut(s)	17, 812
Bsp1286I	GDGCHC	8 cut(s)	276, 373, 638, 2327, 2532, 2799, 2906, 3194
Bsp13I	TCCGGA	2 cut(s)	227, 1891
Bsp1407I	TGTACA	2 cut(s)	1593, 2982
Bsp1720I	GCTNAGC	4 cut(s)	134, 275, 1479, 1543
Bsp68I	TCGCGA	1 cut(s)	1421
BspCNI	CTCAG	7 cut(s)	126, 288, 417, 868, 1146, 1465, 1713
BspEI	TCCGGA	2 cut(s)	227, 1891
BspFNI	CGCG	3 cut(s)	168, 1421, 2802
BspHI	TCATGA	1 cut(s)	72
BspLI	GGNNCC	9 cut(s)	197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
BspMAI	CTGCAG	6 cut(s)	156, 805, 903, 1570, 1756, 2100
BspPI	GGATC	5 cut(s)	892, 1537, 1594, 2631, 2644
BspQI	GCTCTTC	1 cut(s)	1687
BspT104I	TTCGAA	2 cut(s)	17, 812
BspT107I	GGYRCC	2 cut(s)	2625, 3202
BspTI	CTTAAG	1 cut(s)	3173
BsrBI	CCGCTC	2 cut(s)	2020, 3029
BsrDI	GCAATG	3 cut(s)	250, 2824, 3009
BsrFI	RCCGGY	8 cut(s)	293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
BsrGI	TGTACA	2 cut(s)	1593, 2982
BsrI	ACTGG	3 cut(s)	28, 1163, 3103
BssAI	RCCGGY	8 cut(s)	293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
BssECI	CCNNGG	8 cut(s)	175, 1266, 1312, 1810, 2275, 2342, 2473, 2679
BssSI	CACGAG	1 cut(s)	2774
BssT1I	CCWWGG	4 cut(s)	175, 1810, 2473, 2679
Bst2BI	CACGAG	1 cut(s)	2774
Bst2UI	CCWGG	4 cut(s)	755, 1123, 1268, 2277
Bst4CI	ACNGT	5 cut(s)	490, 926, 1775, 2467, 2923
Bst6I	CTCTTC	3 cut(s)	1607, 1687, 2619
BstAFI	CTTAAG	1 cut(s)	3173
BstAPI	GCANNNNNTGC	3 cut(s)	216, 800, 3189
BstAUI	TGTACA	2 cut(s)	1593, 2982
BstBAI	YACGTR	1 cut(s)	2127
BstBI	TTCGAA	2 cut(s)	17, 812
BstDSI	CCRYGG	1 cut(s)	2342
BstEII	GGTNACC	1 cut(s)	2084
BstF5I	GGATG	5 cut(s)	2285, 2336, 2494, 2842, 3040
BstFNI	CGCG	3 cut(s)	168, 1421, 2802
BstH2I	RGCGCY	1 cut(s)	720
BstHHI	GCGC	2 cut(s)	204, 719
BstMAI	GTCTC	2 cut(s)	817, 2701
BstMCI	CGRYCG	3 cut(s)	2046, 2267, 3018
BstNI	CCWGG	4 cut(s)	755, 1123, 1268, 2277
BstNSI	RCATGY	2 cut(s)	1921, 2399
BstPAI	GACNNNNGTC	1 cut(s)	387
BstPI	GGTNACC	1 cut(s)	2084
BstSCI	CCNGG	9 cut(s)	375, 526, 753, 1121, 1266, 1436, 2045, 2275, 3134
BstSFI	CTRYAG	6 cut(s)	152, 801, 899, 1566, 1752, 2096
BstSLI	GKGCMC	2 cut(s)	2799, 3194
BstUI	CGCG	3 cut(s)	168, 1421, 2802
BstV2I	GAAGAC	1 cut(s)	2185
BstX2I	RGATCY	2 cut(s)	1325, 2636
BstXI	CCANNNNNNTGG	1 cut(s)	1094
BstYI	RGATCY	2 cut(s)	1325, 2636
BstZI	CGGCCG	1 cut(s)	2264
BsuI	GTATCC	3 cut(s)	706, 1094, 2139
BtgI	CCRYGG	1 cut(s)	2342
BtgZI	GCGATG	2 cut(s)	1562, 2817
BtrI	CACGTC	1 cut(s)	67
BtsCI	GGATG	5 cut(s)	2285, 2336, 2494, 2842, 3040
BtsI	GCAGTG	1 cut(s)	197
BtsIMutI	CAGTG	3 cut(s)	197, 718, 2472
BtuMI	TCGCGA	1 cut(s)	1421
CaiI	CAGNNNCTG	2 cut(s)	800, 826
CciI	TCATGA	1 cut(s)	72
CfoI	GCGC	2 cut(s)	204, 719
Cfr10I	RCCGGY	8 cut(s)	293, 1076, 1292, 1364, 1635, 2262, 2304, 3115
Cfr13I	GGNCC	9 cut(s)	225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
CseI	GACGC	2 cut(s)	1702, 2061
CspAI	ACCGGT	5 cut(s)	293, 1076, 1292, 1364, 3115
CspCI	CAANNNNNGTGG	2 cut(s)	1365, 1400
EaeI	YGGCCR	3 cut(s)	439, 2264, 3141
EagI	CGGCCG	1 cut(s)	2264
Eam1104I	CTCTTC	3 cut(s)	1607, 1687, 2619
EarI	CTCTTC	3 cut(s)	1607, 1687, 2619
EciI	GGCGGA	2 cut(s)	3167, 3212
Ecl136II	GAGCTC	3 cut(s)	274, 636, 2325
EclXI	CGGCCG	1 cut(s)	2264
Eco130I	CCWWGG	4 cut(s)	175, 1810, 2473, 2679
Eco147I	AGGCCT	1 cut(s)	905
Eco24I	GRGCYC	4 cut(s)	276, 638, 2327, 2532
Eco32I	GATATC	1 cut(s)	2694
Eco47I	GGWCC	7 cut(s)	225, 1154, 1270, 1367, 1633, 2676, 3132
Eco52I	CGGCCG	1 cut(s)	2264
Eco53kI	GAGCTC	3 cut(s)	274, 636, 2325
Eco88I	CYCGRG	3 cut(s)	1705, 2320, 2751
Eco91I	GGTNACC	1 cut(s)	2084
EcoICRI	GAGCTC	3 cut(s)	274, 636, 2325
EcoO109I	RGGNCCY	2 cut(s)	1154, 2676
EcoO65I	GGTNACC	1 cut(s)	2084
EcoRII	CCWGG	4 cut(s)	753, 1121, 1266, 2275
EcoRV	GATATC	1 cut(s)	2694
EcoT14I	CCWWGG	4 cut(s)	175, 1810, 2473, 2679
EcoT38I	GRGCYC	4 cut(s)	276, 638, 2327, 2532
ErhI	CCWWGG	4 cut(s)	175, 1810, 2473, 2679
FalI	AAGNNNNNCTT	4 cut(s)	506, 538, 752, 784
FaqI	GGGAC	6 cut(s)	909, 1520, 1646, 2476, 2689, 2932
FauI	CCCGC	4 cut(s)	879, 1519, 2666, 2908
FauNDI	CATATG	2 cut(s)	1628, 1667
FbaI	TGATCA	4 cut(s)	69, 75, 828, 1513
FblI	GTMKAC	1 cut(s)	2655
FokI	GGATG	5 cut(s)	2292, 2323, 2501, 2849, 3047
FriOI	GRGCYC	4 cut(s)	276, 638, 2327, 2532
FspBI	CTAG	9 cut(s)	545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
GlaI	GCGC	2 cut(s)	203, 718
HaeII	RGCGCY	1 cut(s)	720
HgaI	GACGC	2 cut(s)	1702, 2061
HhaI	GCGC	2 cut(s)	204, 719
Hin6I	GCGC	2 cut(s)	202, 717
HinP1I	GCGC	2 cut(s)	202, 717
HindIII	AAGCTT	1 cut(s)	2513
HphI	GGTGA	8 cut(s)	283, 308, 568, 665, 1054, 2518, 2549, 2588
Hpy99I	CGWCG	1 cut(s)	2971
HpyCH4III	ACNGT	5 cut(s)	490, 926, 1775, 2467, 2923
HpyCH4IV	ACGT	3 cut(s)	66, 2060, 2126
HpySE526I	ACGT	3 cut(s)	66, 2060, 2126
HspAI	GCGC	2 cut(s)	202, 717
Kpn2I	TCCGGA	2 cut(s)	227, 1891
KroI	GCCGGC	1 cut(s)	2262
KroNI	GCCGGC	1 cut(s)	2264
Ksp22I	TGATCA	4 cut(s)	69, 75, 828, 1513
LguI	GCTCTTC	1 cut(s)	1687
LmnI	GCTCC	3 cut(s)	368, 1752, 2941
LweI	GCATC	2 cut(s)	1332, 2872
MaeI	CTAG	9 cut(s)	545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
MaeII	ACGT	3 cut(s)	66, 2060, 2126
MaeIII	GTNAC	8 cut(s)	420, 539, 917, 1709, 2084, 2232, 2608, 2851
MbiI	CCGCTC	2 cut(s)	2020, 3029
MboII	GAAGA	8 cut(s)	364, 526, 1624, 1674, 2185, 2444, 2521, 2606
MflI	RGATCY	2 cut(s)	1325, 2636
MhlI	GDGCHC	8 cut(s)	276, 373, 638, 2327, 2532, 2799, 2906, 3194
MlsI	TGGCCA	1 cut(s)	441
MluNI	TGGCCA	1 cut(s)	441
MlyI	GAGTC	1 cut(s)	397
MmeI	TCCRAC	4 cut(s)	79, 450, 744, 2180
Mox20I	TGGCCA	1 cut(s)	441
MroI	TCCGGA	2 cut(s)	227, 1891
MroNI	GCCGGC	1 cut(s)	2262
MroXI	GAANNNNTTC	2 cut(s)	572, 1249
MscI	TGGCCA	1 cut(s)	441
MseI	TTAA	9 cut(s)	612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
MslI	CAYNNNNRTG	3 cut(s)	2341, 2802, 2909
Msp20I	TGGCCA	1 cut(s)	441
MspA1I	CMGCKG	1 cut(s)	445
MspCI	CTTAAG	1 cut(s)	3173
MspR9I	CCNGG	9 cut(s)	377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
Mva1269I	GAATGC	2 cut(s)	611, 1544
MvaI	CCWGG	4 cut(s)	755, 1123, 1268, 2277
MvnI	CGCG	3 cut(s)	168, 1421, 2802
NaeI	GCCGGC	1 cut(s)	2264
NciI	CCSGG	5 cut(s)	377, 528, 1438, 2047, 3136
NdeI	CATATG	2 cut(s)	1628, 1667
NgoMIV	GCCGGC	1 cut(s)	2262
NlaIV	GGNNCC	9 cut(s)	197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
NmuCI	GTSAC	2 cut(s)	420, 2232
NruI	TCGCGA	1 cut(s)	1421
NspI	RCATGY	2 cut(s)	1921, 2399
NspV	TTCGAA	2 cut(s)	17, 812
OliI	CACNNNNGTG	1 cut(s)	2909
PaeI	GCATGC	1 cut(s)	1921
PaeR7I	CTCGAG	2 cut(s)	2320, 2751
PagI	TCATGA	1 cut(s)	72
PceI	AGGCCT	1 cut(s)	905
PciSI	GCTCTTC	1 cut(s)	1687
PcsI	WCGNNNNNNNCGW	1 cut(s)	2799
PctI	GAATGC	2 cut(s)	611, 1544
PdiI	GCCGGC	1 cut(s)	2264
PdmI	GAANNNNTTC	2 cut(s)	572, 1249
PflMI	CCANNNNNTGG	2 cut(s)	1241, 1267
PfoI	TCCNGGA	2 cut(s)	375, 526
PinAI	ACCGGT	5 cut(s)	293, 1076, 1292, 1364, 3115
PleI	GAGTC	1 cut(s)	396
PpsI	GAGTC	1 cut(s)	396
Ppu21I	YACGTR	1 cut(s)	2127
PpuMI	RGGWCCY	2 cut(s)	1154, 2676
PshAI	GACNNNNGTC	1 cut(s)	387
Psp124BI	GAGCTC	3 cut(s)	276, 638, 2327
Psp1406I	AACGTT	1 cut(s)	2060
Psp5II	RGGWCCY	2 cut(s)	1154, 2676
Psp6I	CCWGG	4 cut(s)	753, 1121, 1266, 2275
PspEI	GGTNACC	1 cut(s)	2084
PspGI	CCWGG	4 cut(s)	753, 1121, 1266, 2275
PspN4I	GGNNCC	9 cut(s)	197, 851, 864, 1634, 2627, 2638, 2677, 3133, 3204
PspPI	GGNCC	9 cut(s)	225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
PspPPI	RGGWCCY	2 cut(s)	1154, 2676
PspXI	VCTCGAGB	2 cut(s)	2320, 2751
PstI	CTGCAG	6 cut(s)	156, 805, 903, 1570, 1756, 2100
PstNI	CAGNNNCTG	2 cut(s)	800, 826
PsuI	RGATCY	2 cut(s)	1325, 2636
PvuII	CAGCTG	1 cut(s)	445
RruI	TCGCGA	1 cut(s)	1421
RseI	CAYNNNNRTG	3 cut(s)	2341, 2802, 2909
SacI	GAGCTC	3 cut(s)	276, 638, 2327
SapI	GCTCTTC	1 cut(s)	1687
SaqAI	TTAA	9 cut(s)	612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
Sau96I	GGNCC	9 cut(s)	225, 863, 1154, 1270, 1367, 1439, 1633, 2676, 3132
ScaI	AGTACT	1 cut(s)	2481
SchI	GAGTC	1 cut(s)	397
ScrFI	CCNGG	9 cut(s)	377, 528, 755, 1123, 1268, 1438, 2047, 2277, 3136
SduI	GDGCHC	8 cut(s)	276, 373, 638, 2327, 2532, 2799, 2906, 3194
SfaNI	GCATC	2 cut(s)	1332, 2872
SfcI	CTRYAG	6 cut(s)	152, 801, 899, 1566, 1752, 2096
Sfr274I	CTCGAG	2 cut(s)	2320, 2751
SfuI	TTCGAA	2 cut(s)	17, 812
SgrAI	CRCCGGYG	2 cut(s)	293, 1076
SinI	GGWCC	7 cut(s)	225, 1154, 1270, 1367, 1633, 2676, 3132
SlaI	CTCGAG	2 cut(s)	2320, 2751
SmiMI	CAYNNNNRTG	3 cut(s)	2341, 2802, 2909
SmlI	CTYRAG	7 cut(s)	385, 1103, 1321, 2320, 2751, 3173, 3248
SmoI	CTYRAG	7 cut(s)	385, 1103, 1321, 2320, 2751, 3173, 3248
SpeI	ACTAGT	1 cut(s)	2650
SphI	GCATGC	1 cut(s)	1921
SseBI	AGGCCT	1 cut(s)	905
SspI	AATATT	3 cut(s)	51, 1831, 1909
SspMI	CTAG	9 cut(s)	545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
SstI	GAGCTC	3 cut(s)	276, 638, 2327
StuI	AGGCCT	1 cut(s)	905
StyD4I	CCNGG	9 cut(s)	375, 526, 753, 1121, 1266, 1436, 2045, 2275, 3134
StyI	CCWWGG	4 cut(s)	175, 1810, 2473, 2679
TaaI	ACNGT	5 cut(s)	490, 926, 1775, 2467, 2923
TaiI	ACGT	3 cut(s)	69, 2063, 2129
TatI	WGTACW	5 cut(s)	3, 1593, 2479, 2661, 2982
TauI	GCSGC	2 cut(s)	1008, 2269
Tru1I	TTAA	9 cut(s)	612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
Tru9I	TTAA	9 cut(s)	612, 1209, 1413, 1644, 1911, 2216, 2361, 3174, 3292
TscAI	CASTG	3 cut(s)	197, 718, 2472
TseFI	GTSAC	2 cut(s)	420, 2232
Tsp45I	GTSAC	2 cut(s)	420, 2232
TspGWI	ACGGA	4 cut(s)	79, 1236, 1705, 3006
TspRI	CASTG	3 cut(s)	197, 718, 2472
Van91I	CCANNNNNTGG	2 cut(s)	1241, 1267
Vha464I	CTTAAG	1 cut(s)	3173
VneI	GTGCAC	2 cut(s)	2795, 3190
VpaK11BI	GGWCC	7 cut(s)	225, 1154, 1270, 1367, 1633, 2676, 3132
XapI	RAATTY	5 cut(s)	231, 1170, 1789, 1887, 2108
XbaI	TCTAGA	1 cut(s)	932
XceI	RCATGY	2 cut(s)	1921, 2399
XhoI	CTCGAG	2 cut(s)	2320, 2751
XmiI	GTMKAC	1 cut(s)	2655
XmnI	GAANNNNTTC	2 cut(s)	572, 1249
XspI	CTAG	9 cut(s)	545, 933, 1305, 1608, 2328, 2651, 2828, 2871, 3236
ZrmI	AGTACT	1 cut(s)	2481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.