MD06G1198600.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004694
Physical Location & Seq
Forward (+)
33258211 .. 33261678
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1198600.v1.1.491

Sequence Viewer

Length: 3270 bp
ATGATCACAGGTACTTGCTCCAACATGTTGCAGATTTCTGAAACAATCTTTGTTGTGGGTGCTTCCGTTGTAGCCGGAGATTCTCTGGACACAGACAGAGAAGTTCTGCTGAGCCTCAAAGCATTTCTGCAGCAAAAGAACCGCGTAAACCAAGGAATATACTCGCAGTGGAACCAGCGCAGCAGCAACCCCTGCGAGTGGCTCGGAATTTCGTGCAGCAATGACAGCAAGAGAGTTAGCCGTGTTGAGCTCTCCGACCAAAAAATCACCGGTGAGATTTTCCGAAACTTCTCTGCCCTTACAGCACTTTCGTACCTCGACCTCTCGAAGAACACAATAAGCGGAGCACTCCTGGAGGACTTGAGTCAATGCCACAGCCTCAGACACCTCAATTTGTCACATAACATTATTGATGGCGAGCTGAACTTGAATGGTTTGAATCAGTTGGAGGTTCTTGATTTGACTGTCAATAGATTTTACGGCGATTTGAAGATGACCTTTCCCGGAGTTTGCAGTAACCTAGTGGTTGCGAATCTTTCAGCGAATAATTTCACCGGAAGGATTGATAACCTGTTTGATGAATGCTACAATTTGCGGTACTTGGATTTGAGCTCAAACTATCTTAGTGGTGAGATATGGAGTGGATTCATCAGGCTTACGGCGTTTTCAATATCCGAAAACTATTTCAGTGGCGCTATTTCGCCTTCTGTTTTCACAAATAATTGTAGCCTGGTAGCTTTGGACTTGTCAGTAAACAGTTTTTCTGGTGCAGTTCCTGCAGAGATTTCGAAATGCCAGAGACTGATCATATTGAATCTTTGGGGGAACCATTTTACGGGGCCAATCCCGCCTGAGATTGGATCAATTTCGGGACTGCAGGGCTTGTTCTTGGGTAACAACAGTTTTTCTAGAGATATTCCTGAATCCCTTTTGGATTTGAGCAGCTTGACATTCTTGGATTTGAGCAGGAACAACTTTCGCGGCAAGATACAAGACATTTTTGGGAGATTCAGACAGGTGAAGTTTATTTTGCTGCACTCAAACGGGTACACCGGTGGGATCCATTCGTCTGGGATTCTCAAGCTACCAAATGTTTCCAGGTTAGACCTCAGCCACAACAATTTTACAGGTCCTCTGCCACTCGAAATTTCTCTGCCGAAGTTGAAGTTATTTGTCCTCGCCTTTAATCAATTCAGCGGAACTATACCACCGGGCTATGGAAACATTTCGACCCTCCAAGCCCTGGACCTTTCCTTCAACAGCCTAACCGGTGCAATTCCTAGCACCCTCGGAAACTTGAGCTCTCTCTTGTGGTTGATGCTTGCCAACAATTCCCTAACCGGTCCAATTCCACACGAATTGGGAAATTGTAGAAGCTTGCTGTGGCTTAATCTCGCGAATAACAAGCTCTCCGGGCCAATCCCATCTCAGCTAACAATGATTGGGAGAAATGTTCAGCCAACATTCGACATGAATAATCGGGACAATGATCAGATCATAGCGGGATCAGGCGAATGCTTAGCGATGAAGAGATGGATTCCTGCAGATTACCCACCATTCAATTTCGTGTACACGATCCTGACTAGGAAGAGTTGTCGAAGCATATGGGACCGGCTGCTTAAAGGAAAAGGCCTATTTCCCATATGTGCAGCTGGCTCTTATGTACGGACACTTCAAATCTCGGGTTACGTTCAACTGAGTGGGAATCAGCTTTCGGGTCAGCTCCCTGCAGATATCGGTACAATGAGCAATTTTAGCATGATAAATTTGGGTGTCAATAGATTCCAAGGCGAACTCCCTGCAAATATTGGTCAGTTGCCACTTGTAGTGTTCAACATCAGCAGGAACAATTTTTCGGGTCAAATTCCAGAGGAGATTGGGAATATTAAGTGCATGCAGAATCTTGATATGTCATATAACAATTTTTCTGGCACGTTTCCTCTGAGCCTGAACGGCTTGAATGAGCTAAGTAAGTTCAACATTTCGTACAATCCGCTCATTTCCGGCATAATTCCTTCGATCGGGCAGTTGGCAACGTTCGAGAAAGAGTCCTATCTCGGTAACCCCCTTCTGCAGCTTCCCGAATTCATCCACAACTCCACGTATCCAGAAAACAAAACGAAAGGGAATCCGAAAAAGCCTACAAAATCTGCTGCACTTATGGTGGTCTTCGGTTTGCTAATTGCTCTCTTAATCTGTGGAGTTTTGTCACTTGTAGCATGCTTATTCGGAAAGAGCCGGCCACAGCCCCCAGCATGCTTGTTGCAGGACATCAAATACCGGCATGACTTGGCCTCGAGCTCTAGCAGTTCATCCCCGTGGCTGTCGGATACTGTTAAGATCATTCGTTTGGACAAAATGGCTTTTACACATGCTGACATTTTGAAAGCTACACGCAATTTTTCGGAAGATAGGATTATTGGGAGTGGAGGGTTTGGGACAGTGTACCAAGGAGTATTGCAGGATGGGAGAGTAGTAGCGGTGAAGAAGCTTCAAAGAGAAGGACTCGAAGGTGAAAGGGAGTTCCGAGCTGAAATGGAGGTTCTTAGTGGGAATGGCATTGGCTGGCCTCACCCAAACCTTGTAACTCTTCACGGCTGGTGCCTTTATGGATCCGAAAAAATACTAGTCTACGAGTACATGGAAGGCGGGACCTTGGAGGATTTGATATCTGATAGAGTGAGACTGACATGGCGAAGGCGAGTTGATGTGGCAGTTGACGTGGCTCGAGCCTTGGTGTTTCTACACCACGAGTGCTTCCCTTCCATCGTGCACCGCGATGTAAAGGCGAGCAATGTGCTGCTAGATAAGGATGGAAAGGCGAGAGTTACAGATTTCGGGCTTGCCAGAATAGTGGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCGGGGACTGTTGGTTATGTCGCTCCCGAATATGGGCAGACATGGCAAGCCACGACGAAAGGAGACGTGTACAGCTACGGAGTTCTGGCAATGGAATTGGCGACCGGAAGGAGAGCGGTGGATGGAGGAGAGGAGTGCCTTGTGGAATGGGCAAGGAGGGTAATGGGAAATGGACGGCAAGGATTCAACCAGTCTGTGATACCGGTTATGCTCATGGGGTCCGGGCTGGCCGATGGGGCGGAGGAGATGTGCGAGCTGCTTAAGGTGGGCATAAAGTGCACGGCGGAGGCACCGCAGTCAAGGCCAAACATGAAGGAGGTTCTAGCTATGCTGCTCAAGATATATAAAGATGAAAACATATATAGTTTACAAAGTAACTAA

Protein Analysis

1090

Amino Acids

119.69

Weight (kDa)

6.8

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 73 1.1e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 95 - 135 7.3e-07 Leucine rich repeat
LRR_8 PF13855 411 - 470 7e-09 Leucine rich repeat
Pkinase PF00069 802 - 1071 3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 804 - 1073 2.1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2601, 3178
AccB7I CCANNNNNTGG 1 cut(s) 1243
AccBSI CCGCTC 2 cut(s) 1996, 3005
AccI GTMKAC 1 cut(s) 2631
AccII CGCG 4 cut(s) 144, 981, 1397, 2778
AclI AACGTT 1 cut(s) 2036
AclWI GGATC 7 cut(s) 868, 1054, 1067, 1513, 1570, 2607, 2620
AcoI YGGCCR 2 cut(s) 2240, 3117
AcsI RAATTY 5 cut(s) 207, 1146, 1765, 1863, 2084
AfiI CCNNNNNNNGG 8 cut(s) 198, 835, 857, 1217, 1243, 2021, 2921, 2922
AflII CTTAAG 1 cut(s) 3149
AflIII ACRYGT 2 cut(s) 24, 2955
AgeI ACCGGT 5 cut(s) 269, 1052, 1268, 1340, 3091
AhlI ACTAGT 1 cut(s) 2626
AjiI CACGTC 2 cut(s) 2722, 2956
AjnI CCWGG 4 cut(s) 351, 729, 1097, 1242
AleI CACNNNNGTG 1 cut(s) 2885
Alw21I GWGCWC 8 cut(s) 252, 349, 614, 1304, 2303, 2775, 2882, 3170
Alw26I GTCTC 3 cut(s) 793, 2677, 2946
Alw44I GTGCAC 2 cut(s) 2771, 3166
AlwI GGATC 7 cut(s) 868, 1054, 1067, 1513, 1570, 2607, 2620
AlwNI CAGNNNCTG 2 cut(s) 776, 802
Ama87I CYCGRG 3 cut(s) 1681, 2296, 2727
AoxI GGCC 8 cut(s) 839, 1415, 1630, 2240, 2292, 2567, 3117, 3191
ApaLI GTGCAC 2 cut(s) 2771, 3166
ApoI RAATTY 5 cut(s) 207, 1146, 1765, 1863, 2084
AsiGI ACCGGT 5 cut(s) 269, 1052, 1268, 1340, 3091
Asp700I GAANNNNTTC 2 cut(s) 548, 1225
AspLEI GCGC 2 cut(s) 180, 695
AspS9I GGNCC 8 cut(s) 839, 1130, 1246, 1343, 1415, 1609, 2652, 3108
AsuC2I CCSGG 4 cut(s) 504, 1212, 1414, 3112
AsuHPI GGTGA 8 cut(s) 259, 284, 544, 641, 1030, 2494, 2525, 2564
AsuII TTCGAA 1 cut(s) 788
AvaI CYCGRG 3 cut(s) 1681, 2296, 2727
AvaII GGWCC 6 cut(s) 1130, 1246, 1343, 1609, 2652, 3108
BaeGI GKGCMC 2 cut(s) 2775, 3170
BaeI ACNNNNGTAYC 4 cut(s) 296, 329, 1039, 1072
BamHI GGATCC 2 cut(s) 1059, 2612
BanI GGYRCC 2 cut(s) 2601, 3178
BanII GRGCYC 4 cut(s) 252, 614, 1304, 2303
BarI GAAGNNNNNNTAC 2 cut(s) 1656, 1688
BauI CACGAG 1 cut(s) 2750
BbsI GAAGAC 1 cut(s) 2161
Bbv12I GWGCWC 8 cut(s) 252, 349, 614, 1304, 2303, 2775, 2882, 3170
BbvCI CCTCAGC 1 cut(s) 1109
BccI CCATC 8 cut(s) 407, 1432, 1527, 2459, 2774, 2807, 3005, 3116
BceAI ACGGC 7 cut(s) 225, 496, 675, 1969, 2611, 3080, 3186
BcgI CGANNNNNNTGC 6 cut(s) 768, 802, 1781, 1815, 2779, 2813
BciT130I CCWGG 4 cut(s) 353, 731, 1099, 1244
BciVI GTATCC 2 cut(s) 2115, 2323
BclI TGATCA 3 cut(s) 3, 804, 1489
BcnI CCSGG 4 cut(s) 504, 1212, 1414, 3112
BcoDI GTCTC 3 cut(s) 793, 2677, 2946
BcuI ACTAGT 1 cut(s) 2626
BfaI CTAG 8 cut(s) 521, 909, 1281, 1584, 2304, 2627, 2804, 3212
BfmI CTRYAG 6 cut(s) 128, 777, 875, 1542, 1728, 2072
BfoI RGCGCY 1 cut(s) 696
BfrI CTTAAG 1 cut(s) 3149
BfuI GTATCC 2 cut(s) 2115, 2323
BglI GCCNNNNNGGC 2 cut(s) 1953, 3125
BlpI GCTNAGC 2 cut(s) 110, 1519
Bme1390I CCNGG 8 cut(s) 353, 504, 731, 1099, 1212, 1244, 1414, 3112
Bme18I GGWCC 6 cut(s) 1130, 1246, 1343, 1609, 2652, 3108
BmeT110I CYCGRG 3 cut(s) 1681, 2296, 2727
BmgBI CACGTC 2 cut(s) 2722, 2956
BmgT120I GGNCC 8 cut(s) 839, 1130, 1246, 1343, 1415, 1609, 2652, 3108
BmrFI CCNGG 8 cut(s) 353, 504, 731, 1099, 1212, 1244, 1414, 3112
BmsI GCATC 2 cut(s) 1308, 2848
BoxI GACNNNNGTC 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 2161
BplI GAGNNNNNCTC 2 cut(s) 2490, 2522
BpmI CTGGAG 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 1109
Bpu1102I GCTNAGC 2 cut(s) 110, 1519
Bpu14I TTCGAA 1 cut(s) 788
BpuEI CTTGAG 4 cut(s) 382, 1064, 1318, 3209
BpuMI CCSGG 4 cut(s) 504, 1212, 1414, 3112
BsaAI YACGTR 1 cut(s) 2103
BsaJI CCNNGG 8 cut(s) 151, 1242, 1288, 1786, 2318, 2449, 2655, 2733
BsaWI WCCGGW 7 cut(s) 269, 554, 1052, 1268, 1340, 2993, 3091
BsaXI ACNNNNNCTCC 4 cut(s) 1394, 1424, 3122, 3152
Bsc4I CCNNNNNNNGG 8 cut(s) 198, 835, 857, 1217, 1243, 2021, 2921, 2922
Bse118I RCCGGY 8 cut(s) 269, 1052, 1268, 1340, 1611, 2238, 2280, 3091
Bse1I ACTGG 1 cut(s) 3079
Bse3DI GCAATG 3 cut(s) 226, 2800, 2985
BseBI CCWGG 4 cut(s) 353, 731, 1099, 1244
BseDI CCNNGG 8 cut(s) 151, 1242, 1288, 1786, 2318, 2449, 2655, 2733
BseGI GGATG 6 cut(s) 2088, 2312, 2470, 2818, 2863, 3016
BseLI CCNNNNNNNGG 8 cut(s) 198, 835, 857, 1217, 1243, 2021, 2921, 2922
BseMI GCAATG 3 cut(s) 226, 2800, 2985
BseMII CTCAG 7 cut(s) 101, 394, 843, 1123, 1442, 1688, 1934
BseNI ACTGG 1 cut(s) 3079
BseRI GAGGAG 4 cut(s) 1886, 3030, 3035, 3146
BseSI GKGCMC 2 cut(s) 2775, 3170
BseYI CCCAGC 1 cut(s) 2251
BsgI GTGCAG 5 cut(s) 235, 789, 1019, 1668, 2139
Bsh1236I CGCG 4 cut(s) 144, 981, 1397, 2778
Bsh1285I CGRYCG 2 cut(s) 2022, 2994
BshFI GGCC 8 cut(s) 841, 1417, 1632, 2242, 2294, 2569, 3119, 3193
BshNI GGYRCC 2 cut(s) 2601, 3178
BshTI ACCGGT 5 cut(s) 269, 1052, 1268, 1340, 3091
BsiEI CGRYCG 2 cut(s) 2022, 2994
BsiHKAI GWGCWC 8 cut(s) 252, 349, 614, 1304, 2303, 2775, 2882, 3170
BsiHKCI CYCGRG 3 cut(s) 1681, 2296, 2727
BslFI GGGAC 6 cut(s) 885, 1496, 1622, 2452, 2665, 2908
BslI CCNNNNNNNGG 8 cut(s) 198, 835, 857, 1217, 1243, 2021, 2921, 2922
BsmAI GTCTC 3 cut(s) 793, 2677, 2946
BsmBI CGTCTC 1 cut(s) 2946
BsmFI GGGAC 6 cut(s) 885, 1496, 1622, 2452, 2665, 2908
BsmI GAATGC 2 cut(s) 587, 1520
BsnI GGCC 8 cut(s) 841, 1417, 1632, 2242, 2294, 2569, 3119, 3193
BsoBI CYCGRG 3 cut(s) 1681, 2296, 2727
Bsp119I TTCGAA 1 cut(s) 788
Bsp1286I GDGCHC 8 cut(s) 252, 349, 614, 1304, 2303, 2775, 2882, 3170
Bsp1407I TGTACA 2 cut(s) 1569, 2958
Bsp1720I GCTNAGC 2 cut(s) 110, 1519
Bsp68I TCGCGA 1 cut(s) 1397
BspANI GGCC 8 cut(s) 841, 1417, 1632, 2242, 2294, 2569, 3119, 3193
BspCNI CTCAG 7 cut(s) 102, 393, 844, 1122, 1441, 1689, 1935
BspFNI CGCG 4 cut(s) 144, 981, 1397, 2778
BspMAI CTGCAG 6 cut(s) 132, 781, 879, 1546, 1732, 2076
BspPI GGATC 7 cut(s) 868, 1054, 1067, 1513, 1570, 2607, 2620
BspT104I TTCGAA 1 cut(s) 788
BspT107I GGYRCC 2 cut(s) 2601, 3178
BspTI CTTAAG 1 cut(s) 3149
BsrBI CCGCTC 2 cut(s) 1996, 3005
BsrDI GCAATG 3 cut(s) 226, 2800, 2985
BsrFI RCCGGY 8 cut(s) 269, 1052, 1268, 1340, 1611, 2238, 2280, 3091
BsrGI TGTACA 2 cut(s) 1569, 2958
BsrI ACTGG 1 cut(s) 3079
BssAI RCCGGY 8 cut(s) 269, 1052, 1268, 1340, 1611, 2238, 2280, 3091
BssECI CCNNGG 8 cut(s) 151, 1242, 1288, 1786, 2318, 2449, 2655, 2733
BssSI CACGAG 1 cut(s) 2750
BssT1I CCWWGG 5 cut(s) 151, 1786, 2449, 2655, 2733
Bst2BI CACGAG 1 cut(s) 2750
Bst2UI CCWGG 4 cut(s) 353, 731, 1099, 1244
Bst4CI ACNGT 6 cut(s) 466, 758, 902, 2335, 2443, 2899
Bst6I CTCTTC 3 cut(s) 1523, 1583, 2595
BstAFI CTTAAG 1 cut(s) 3149
BstAPI GCANNNNNTGC 3 cut(s) 192, 776, 3165
BstAUI TGTACA 2 cut(s) 1569, 2958
BstBAI YACGTR 1 cut(s) 2103
BstBI TTCGAA 1 cut(s) 788
BstDSI CCRYGG 1 cut(s) 2318
BstEII GGTNACC 1 cut(s) 2060
BstF5I GGATG 6 cut(s) 2088, 2312, 2470, 2818, 2863, 3016
BstFNI CGCG 4 cut(s) 144, 981, 1397, 2778
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 2 cut(s) 180, 695
BstMAI GTCTC 3 cut(s) 793, 2677, 2946
BstMCI CGRYCG 2 cut(s) 2022, 2994
BstNI CCWGG 4 cut(s) 353, 731, 1099, 1244
BstNSI RCATGY 5 cut(s) 28, 1897, 2223, 2259, 2375
BstPAI GACNNNNGTC 1 cut(s) 363
BstPI GGTNACC 1 cut(s) 2060
BstSCI CCNGG 8 cut(s) 351, 502, 729, 1097, 1210, 1242, 1412, 3110
BstSFI CTRYAG 6 cut(s) 128, 777, 875, 1542, 1728, 2072
BstSLI GKGCMC 2 cut(s) 2775, 3170
BstUI CGCG 4 cut(s) 144, 981, 1397, 2778
BstV2I GAAGAC 1 cut(s) 2161
BstX2I RGATCY 2 cut(s) 1059, 2612
BstXI CCANNNNNNTGG 2 cut(s) 1070, 2854
BstYI RGATCY 2 cut(s) 1059, 2612
BsuI GTATCC 2 cut(s) 2115, 2323
BsuRI GGCC 8 cut(s) 841, 1417, 1632, 2242, 2294, 2569, 3119, 3193
BtgI CCRYGG 1 cut(s) 2318
BtgZI GCGATG 2 cut(s) 1538, 2793
BtrI CACGTC 2 cut(s) 2722, 2956
BtsCI GGATG 6 cut(s) 2088, 2312, 2470, 2818, 2863, 3016
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 3 cut(s) 173, 694, 2448
BtuMI TCGCGA 1 cut(s) 1397
CaiI CAGNNNCTG 2 cut(s) 776, 802
CfoI GCGC 2 cut(s) 180, 695
Cfr10I RCCGGY 8 cut(s) 269, 1052, 1268, 1340, 1611, 2238, 2280, 3091
Cfr13I GGNCC 8 cut(s) 839, 1130, 1246, 1343, 1415, 1609, 2652, 3108
CspAI ACCGGT 5 cut(s) 269, 1052, 1268, 1340, 3091
CspCI CAANNNNNGTGG 2 cut(s) 1341, 1376
EaeI YGGCCR 2 cut(s) 2240, 3117
Eam1104I CTCTTC 3 cut(s) 1523, 1583, 2595
EarI CTCTTC 3 cut(s) 1523, 1583, 2595
EciI GGCGGA 2 cut(s) 3143, 3188
Ecl136II GAGCTC 4 cut(s) 250, 612, 1302, 2301
Eco130I CCWWGG 5 cut(s) 151, 1786, 2449, 2655, 2733
Eco147I AGGCCT 1 cut(s) 1632
Eco24I GRGCYC 4 cut(s) 252, 614, 1304, 2303
Eco32I GATATC 2 cut(s) 1735, 2670
Eco47I GGWCC 6 cut(s) 1130, 1246, 1343, 1609, 2652, 3108
Eco53kI GAGCTC 4 cut(s) 250, 612, 1302, 2301
Eco88I CYCGRG 3 cut(s) 1681, 2296, 2727
Eco91I GGTNACC 1 cut(s) 2060
EcoICRI GAGCTC 4 cut(s) 250, 612, 1302, 2301
EcoO109I RGGNCCY 2 cut(s) 1130, 2652
EcoO65I GGTNACC 1 cut(s) 2060
EcoRI GAATTC 1 cut(s) 2084
EcoRII CCWGG 4 cut(s) 351, 729, 1097, 1242
EcoRV GATATC 2 cut(s) 1735, 2670
EcoT14I CCWWGG 5 cut(s) 151, 1786, 2449, 2655, 2733
EcoT38I GRGCYC 4 cut(s) 252, 614, 1304, 2303
ErhI CCWWGG 5 cut(s) 151, 1786, 2449, 2655, 2733
Esp3I CGTCTC 1 cut(s) 2946
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 6 cut(s) 885, 1496, 1622, 2452, 2665, 2908
FauI CCCGC 4 cut(s) 855, 1495, 2642, 2884
FauNDI CATATG 2 cut(s) 1604, 1643
FbaI TGATCA 3 cut(s) 3, 804, 1489
FblI GTMKAC 1 cut(s) 2631
FokI GGATG 6 cut(s) 2075, 2299, 2477, 2825, 2870, 3023
FriOI GRGCYC 4 cut(s) 252, 614, 1304, 2303
FspBI CTAG 8 cut(s) 521, 909, 1281, 1584, 2304, 2627, 2804, 3212
GlaI GCGC 2 cut(s) 179, 694
GsaI CCCAGC 1 cut(s) 2255
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 1 cut(s) 696
HaeIII GGCC 8 cut(s) 841, 1417, 1632, 2242, 2294, 2569, 3119, 3193
HhaI GCGC 2 cut(s) 180, 695
Hin6I GCGC 2 cut(s) 178, 693
HinP1I GCGC 2 cut(s) 178, 693
HincII GTYRAC 1 cut(s) 2719
HindII GTYRAC 1 cut(s) 2719
HindIII AAGCTT 2 cut(s) 1375, 2489
HphI GGTGA 8 cut(s) 259, 284, 544, 641, 1030, 2494, 2525, 2564
Hpy99I CGWCG 1 cut(s) 2947
HpyCH4III ACNGT 6 cut(s) 466, 758, 902, 2335, 2443, 2899
HpyCH4IV ACGT 6 cut(s) 1689, 1934, 2036, 2102, 2721, 2955
HpySE526I ACGT 6 cut(s) 1689, 1934, 2036, 2102, 2721, 2955
HspAI GCGC 2 cut(s) 178, 693
KroI GCCGGC 1 cut(s) 2238
KroNI GCCGGC 1 cut(s) 2240
Ksp22I TGATCA 3 cut(s) 3, 804, 1489
LmnI GCTCC 4 cut(s) 23, 344, 1728, 2917
LweI GCATC 2 cut(s) 1308, 2848
MaeI CTAG 8 cut(s) 521, 909, 1281, 1584, 2304, 2627, 2804, 3212
MaeII ACGT 6 cut(s) 1689, 1934, 2036, 2102, 2721, 2955
MaeIII GTNAC 9 cut(s) 396, 515, 893, 1685, 2060, 2208, 2584, 2827, 3263
MbiI CCGCTC 2 cut(s) 1996, 3005
MboII GAAGA 8 cut(s) 340, 502, 1540, 1600, 2161, 2420, 2497, 2582
MflI RGATCY 2 cut(s) 1059, 2612
MhlI GDGCHC 8 cut(s) 252, 349, 614, 1304, 2303, 2775, 2882, 3170
MlyI GAGTC 3 cut(s) 373, 2057, 2499
MmeI TCCRAC 4 cut(s) 45, 279, 426, 2307
MroNI GCCGGC 1 cut(s) 2238
MroXI GAANNNNTTC 2 cut(s) 548, 1225
MseI TTAA 7 cut(s) 1185, 1389, 1620, 1887, 2192, 2337, 3150
MslI CAYNNNNRTG 3 cut(s) 2317, 2778, 2885
MspA1I CMGCKG 2 cut(s) 1197, 1652
MspCI CTTAAG 1 cut(s) 3149
MspR9I CCNGG 8 cut(s) 353, 504, 731, 1099, 1212, 1244, 1414, 3112
Mva1269I GAATGC 2 cut(s) 587, 1520
MvaI CCWGG 4 cut(s) 353, 731, 1099, 1244
MvnI CGCG 4 cut(s) 144, 981, 1397, 2778
NaeI GCCGGC 1 cut(s) 2240
NciI CCSGG 4 cut(s) 504, 1212, 1414, 3112
NdeI CATATG 2 cut(s) 1604, 1643
NgoMIV GCCGGC 1 cut(s) 2238
NmuCI GTSAC 2 cut(s) 396, 2208
NruI TCGCGA 1 cut(s) 1397
NspI RCATGY 5 cut(s) 28, 1897, 2223, 2259, 2375
NspV TTCGAA 1 cut(s) 788
OliI CACNNNNGTG 1 cut(s) 2885
PaeI GCATGC 3 cut(s) 1897, 2223, 2259
PaeR7I CTCGAG 2 cut(s) 2296, 2727
PceI AGGCCT 1 cut(s) 1632
PciI ACATGT 1 cut(s) 24
PcsI WCGNNNNNNNCGW 1 cut(s) 2775
PctI GAATGC 2 cut(s) 587, 1520
PdiI GCCGGC 1 cut(s) 2240
PdmI GAANNNNTTC 2 cut(s) 548, 1225
PflMI CCANNNNNTGG 1 cut(s) 1243
PfoI TCCNGGA 2 cut(s) 351, 502
PinAI ACCGGT 5 cut(s) 269, 1052, 1268, 1340, 3091
Ple19I CGATCG 1 cut(s) 2022
PleI GAGTC 3 cut(s) 372, 2056, 2499
PpsI GAGTC 3 cut(s) 372, 2056, 2499
Ppu21I YACGTR 1 cut(s) 2103
PpuMI RGGWCCY 2 cut(s) 1130, 2652
PscI ACATGT 1 cut(s) 24
PshAI GACNNNNGTC 1 cut(s) 363
Psp124BI GAGCTC 4 cut(s) 252, 614, 1304, 2303
Psp1406I AACGTT 1 cut(s) 2036
Psp5II RGGWCCY 2 cut(s) 1130, 2652
Psp6I CCWGG 4 cut(s) 351, 729, 1097, 1242
PspEI GGTNACC 1 cut(s) 2060
PspFI CCCAGC 1 cut(s) 2251
PspGI CCWGG 4 cut(s) 351, 729, 1097, 1242
PspPI GGNCC 8 cut(s) 839, 1130, 1246, 1343, 1415, 1609, 2652, 3108
PspPPI RGGWCCY 2 cut(s) 1130, 2652
PspXI VCTCGAGB 2 cut(s) 2296, 2727
PstI CTGCAG 6 cut(s) 132, 781, 879, 1546, 1732, 2076
PstNI CAGNNNCTG 2 cut(s) 776, 802
PsuI RGATCY 2 cut(s) 1059, 2612
PvuI CGATCG 1 cut(s) 2022
PvuII CAGCTG 1 cut(s) 1652
RruI TCGCGA 1 cut(s) 1397
RseI CAYNNNNRTG 3 cut(s) 2317, 2778, 2885
SacI GAGCTC 4 cut(s) 252, 614, 1304, 2303
SaqAI TTAA 7 cut(s) 1185, 1389, 1620, 1887, 2192, 2337, 3150
Sau96I GGNCC 8 cut(s) 839, 1130, 1246, 1343, 1415, 1609, 2652, 3108
SchI GAGTC 3 cut(s) 373, 2057, 2499
ScrFI CCNGG 8 cut(s) 353, 504, 731, 1099, 1212, 1244, 1414, 3112
SduI GDGCHC 8 cut(s) 252, 349, 614, 1304, 2303, 2775, 2882, 3170
SfaNI GCATC 2 cut(s) 1308, 2848
SfcI CTRYAG 6 cut(s) 128, 777, 875, 1542, 1728, 2072
Sfr274I CTCGAG 2 cut(s) 2296, 2727
SfuI TTCGAA 1 cut(s) 788
SgrAI CRCCGGYG 2 cut(s) 269, 1052
SinI GGWCC 6 cut(s) 1130, 1246, 1343, 1609, 2652, 3108
SlaI CTCGAG 2 cut(s) 2296, 2727
SmiMI CAYNNNNRTG 3 cut(s) 2317, 2778, 2885
SmlI CTYRAG 7 cut(s) 361, 1079, 1297, 2296, 2727, 3149, 3224
SmoI CTYRAG 7 cut(s) 361, 1079, 1297, 2296, 2727, 3149, 3224
SpeI ACTAGT 1 cut(s) 2626
SphI GCATGC 3 cut(s) 1897, 2223, 2259
SseBI AGGCCT 1 cut(s) 1632
SspI AATATT 2 cut(s) 1807, 1885
SspMI CTAG 8 cut(s) 521, 909, 1281, 1584, 2304, 2627, 2804, 3212
SstI GAGCTC 4 cut(s) 252, 614, 1304, 2303
StuI AGGCCT 1 cut(s) 1632
StyD4I CCNGG 8 cut(s) 351, 502, 729, 1097, 1210, 1242, 1412, 3110
StyI CCWWGG 5 cut(s) 151, 1786, 2449, 2655, 2733
TaaI ACNGT 6 cut(s) 466, 758, 902, 2335, 2443, 2899
TaiI ACGT 6 cut(s) 1692, 1937, 2039, 2105, 2724, 2958
TatI WGTACW 3 cut(s) 1569, 2637, 2958
TauI GCSGC 1 cut(s) 984
Tru1I TTAA 7 cut(s) 1185, 1389, 1620, 1887, 2192, 2337, 3150
Tru9I TTAA 7 cut(s) 1185, 1389, 1620, 1887, 2192, 2337, 3150
TscAI CASTG 3 cut(s) 173, 694, 2448
TseFI GTSAC 2 cut(s) 396, 2208
Tsp45I GTSAC 2 cut(s) 396, 2208
TspDTI ATGAA 8 cut(s) 594, 637, 1487, 1541, 2077, 2301, 3215, 3255
TspGWI ACGGA 3 cut(s) 55, 1681, 2982
TspRI CASTG 3 cut(s) 173, 694, 2448
Van91I CCANNNNNTGG 1 cut(s) 1243
Vha464I CTTAAG 1 cut(s) 3149
VneI GTGCAC 2 cut(s) 2771, 3166
VpaK11BI GGWCC 6 cut(s) 1130, 1246, 1343, 1609, 2652, 3108
XapI RAATTY 5 cut(s) 207, 1146, 1765, 1863, 2084
XbaI TCTAGA 1 cut(s) 908
XceI RCATGY 5 cut(s) 28, 1897, 2223, 2259, 2375
XhoI CTCGAG 2 cut(s) 2296, 2727
XmiI GTMKAC 1 cut(s) 2631
XmnI GAANNNNTTC 2 cut(s) 548, 1225
XspI CTAG 8 cut(s) 521, 909, 1281, 1584, 2304, 2627, 2804, 3212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.