Rroxscaffold_3G00271380
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004694
Physical Location & Seq
Reverse (-)
63728944 .. 63732128
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271380.1

Sequence Viewer

Length: 3108 bp
ATGGAAGTTCTGCTGAGCCTTAAAGCTTTTCTTCAAGAACAAAACAGAGTAAACCAAGGCCAATACTCAAAATGGAACCAGAGCATTAGCCCCTGTCGTTGGCCTAGAATCAAATGCAATGGTGACAGTAGAGTTACTGGAATCGATCTCTCCGAAAGCAAAATCTCCGGTGAGTTTTTCAGAAACTTCTCTGCCTTGACACAGCTTTCGGACCTGGACTTGTCCGACAACACTCTAGGTGGAGCTATCCCGGAGGACTTGAGTCAATGCAAGAGCCTAAAGTACCTGAATTTGTCACATAACATTCTAGAAGATGAGCTTCCTGGAATTTGCAAGAACTTGGTGGTGGCAAATGTTTCATCAAACAACTTTACTGGTAGGATTGACCAGTACTTTGATGAGTGCTCGAAATTGCAGTACTTGGATTTGAGCACAAACAAATTTAGTGGTGATTTGTGGGTTGGATTCACAAGGCTTACGGAGTTTTCGGCGTCTGAGAATCACCTCAGTGGGGCTATTTTGCCTTCTATTTTCAATGGCAGTTGTAACTTGGTGATTTTGGACCTGTCACAGAACAATTTCTCCGGTGGAGTTCCAAAGGAGATTTCAAATTGTCGGAATTTGGTTTCGTTGAATGTGTGGGGGAACAAATTTACAGGGCAAATTCCATCTGAGATTGGAAGCATTTCGAGTCTGGAGTCTTTGTTCTTTGGCAATAACAGTTTTTCTAGAGTGATCCCGGAAGCCCTTTTGAATTTGAGCAACTTGGAGTTCTTGGATTTGAGCAGGAACAATTTTGGAGGAGATATACAGGATATTTTTGGGAGATTTAAGCAGGTGGAGATTCTTGTGCTGCACTCAAATTCATACACAGGTGGTATAAATACATCTGGAATTCTCCAGCTACCTAATGTTTATCGGTTAGATCTCAGTAACAACAATTTTACAGGTCCTTTACCGGTTGAAATTGCTAAAATGCCAAGCTTGAAGTACTTGTTCCTTGCCCACAATCAATTCAGTGGAACTATACCACCAGAGTATGGAAATTGGCAAAAGCTCCAAGCGCTGGACCTTTCTTTCAACAGACTAACCGGGTCAATCCCCGCCACCTTAGGAAAGTTGAGATCTCTCTTGTGGTTGATGCTTGCCAATAATTCTCTAACAGGTCCGATTCCTAGGGAGTTAGGGAATTGTACTAGTTTGTTGTGGCTGAACCTTTCCAACAACAAGCTCAATGGAACAATCCCATCTGAGTTGAAGAATATTGGGACAAATGCCGGTCCAACATTTGAATCTAACAAAGATATCAAGGATGAGATCGTAGCTGGATCCGGGGAGTGCTTAGCAATGAAGAGATGGATTCCAGCAGACTACCCTCCATTCAGTTTTGTGTACACAATCCTCACAACGAAGAGTTGTCGAAGTATATGGGATCGGCTACTTAAAGGAAATGGCCTTTTCCCCATATGTGTTGCTGGTTCTGCTGTAAGGACACTGCAAATCTCAGGTTATGTACAACTGAGTGGAAACCAGCTTTCAGGTCAGGTCCCTCCAGAGATTGGGGAGATGCGCAATTTTAGTATGATGAATTTGGCTTTCAATCAATTAAGTGGAGAACTCCCTGCCGGTATTGGACAAATGCCACTCGTAGTGTTTAACCTCAGTAAGAACAGTTTCTCGGGCGAAATCCCCATGGAAATTGGGAACAGTACGTGTATGCAGAATCTTGATTTGTCCTACAATAACTTTTCTGGTACATTTCCTGTAAGCTTAAACAGCTTGACTGAGCTAAGCAAGTTCAATATTTCATTCAATCCACTCATCTCTGGTGTTATTCCTTCAAGTGGCCAATTGGCAACGTTTGAAAAGGAGTCCTACCTTGGTGACCCCCTCCTTATTCTTCCGAGTTTTCTCACCGACTCCACGAATAATTCAGCAAACAAGACAACTGGAAACCAAAAAAAGCCTGCAAAGCTTGGTGCTAATTTGGTGTTTCTAGTGCTAGTACTGACTTTCTTGATATGTGGAGTCTTTTCACTTACGATGTGCTTATTTATGAAGAGCCCAGCAGATGAGCCACAAGGGTATCTGCTGCCACACGGCAAATACCGGCATGACCTTGCATCAAGTTCAAACTCTTCATCACCTTGGCTATCGGATACTGTCAAGGTCATTCGACTGGACAAAACAGCTTTCACACATGCTGACATTTTGAAGGCCACAGGCAATTTTTCAGAAGGGAGGATCATAGGGAGGGGAGGGTTTGGAACAGTGTACCAAGGAGTGTTGCCTGATGGGAGAGAAGTAGCAGTGAAGAAGCTTCAAAGAGAAGGACTTGAAGGTGAAAGGGAATTCAGAGCTGAAATGGAAGTTCTTACTGGAAATGGCTTTGGTTGGCCTCACCCAAATCTTGTGCAACTTCATGGTTGGTGTCATGATGGTTCAGAGAAAATATTAGTCTATGAGTACATGGAAGGTGGGAGCTTGGAGGATATTATATGTGACAAAGTAAGACTAAGATGGCGCAGACGGATTGATTTAGCAATTGATGTGGCTCGAGCCTTGATGTTTCTACACCATGAGTGCTTCCCTGCTATCGTGCACCGTGATGTGAAGGCTAGCAATGTGCTGCTAGATAAGGATGGGAAGGCAAGAGTGACAGATTTTGGGCTGGCTAGAATTGTTGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCTGGAACTGTTGGTTATGTTGCACCAGAATATGGGCAGACATGGCAAGCCACAACGAAGGGCGATGTGTACAGTTATGGAGTCCTAGCAATGGAACTGGCAACTGGGAGGAGAGCAGTGGATGGAGGAGAGGAGTGTCTAGTGGAATGGGCCAGAAGGGTGATGGGAAATGGACGGCTAGGATTCAATCGTTCTGTGATGCCGGTTGTGCTTATGGGGTCTGGACTAGTTGATGGGGCAGAAGAGATGTGTGAGTTACTAAGGGTTGGCATAAAGTGCACAGCTGAGGCACCACAGTCTAGACCAAACATGAAGGAAGTTCTAGCTATGCTGATAAAGATAGCCAATATCCAAGGGGATTTTAGTTGTGGTTCTCCTCCTTCATTTTGA

Protein Analysis

1035

Amino Acids

113.59

Weight (kDa)

5.98

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 46 - 101 8.9e-07 Leucine rich repeat
LRR_8 PF13855 206 - 266 1.1e-06 Leucine rich repeat
LRR_8 PF13855 279 - 339 6.5e-08 Leucine rich repeat
LRR_14 PF23598 322 - 410 1.9e-08 Leucine-rich repeat region
LRR_8 PF13855 352 - 411 1.3e-08 Leucine rich repeat
Pkinase PF00069 745 - 1014 9.2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 747 - 1016 1.2e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 826
Acc16I TGCGCA 1 cut(s) 1571
Acc36I ACCTGC 1 cut(s) 826
AccB1I GGYRCC 1 cut(s) 3007
AccB7I CCANNNNNTGG 4 cut(s) 1040, 1066, 1559, 2750
AciI CCGC 1 cut(s) 1104
AclI AACGTT 1 cut(s) 1859
AclWI GGATC 5 cut(s) 730, 1323, 1336, 1440, 2252
AcoI YGGCCR 1 cut(s) 1846
AcyI GRCGYC 1 cut(s) 491
AfeI AGCGCT 1 cut(s) 1065
AfiI CCNNNNNNNGG 8 cut(s) 99, 511, 1040, 1066, 1559, 1844, 2750, 2809
AflIII ACRYGT 1 cut(s) 1712
AgeI ACCGGT 1 cut(s) 958
AhlI ACTAGT 2 cut(s) 1196, 2944
AjnI CCWGG 2 cut(s) 213, 322
AjuI GAANNNNNNNTTGG 2 cut(s) 589, 621
AleI CACNNNNGTG 2 cut(s) 507, 2714
AloI GAACNNNNNNTCC 4 cut(s) 566, 598, 689, 721
Alw21I GWGCWC 5 cut(s) 407, 434, 2604, 2711, 2999
Alw44I GTGCAC 2 cut(s) 2600, 2995
AlwI GGATC 5 cut(s) 730, 1323, 1336, 1440, 2252
Ama87I CYCGRG 2 cut(s) 1678, 2556
Aor51HI AGCGCT 1 cut(s) 1065
AoxI GGCC 7 cut(s) 58, 101, 1453, 1846, 2217, 2396, 2868
ApaLI GTGCAC 2 cut(s) 2600, 2995
ApeKI GCWGC 3 cut(s) 853, 2092, 2629
ArsI GACNNNNNNTTYG 4 cut(s) 190, 222, 377, 409
AsiGI ACCGGT 1 cut(s) 958
Asp700I GAANNNNTTC 3 cut(s) 578, 685, 1673
AspA2I CCTAGG 1 cut(s) 1175
AspLEI GCGC 3 cut(s) 1066, 1572, 2526
AspS9I GGNCC 8 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546, 2868
AsuC2I CCSGG 4 cut(s) 251, 740, 1093, 1333
AsuNHI GCTAGC 1 cut(s) 2618
AvaI CYCGRG 2 cut(s) 1678, 2556
AvaII GGWCC 7 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546
AvrII CCTAGG 1 cut(s) 1175
AxyI CCTNAGG 1 cut(s) 1111
BaeGI GKGCMC 2 cut(s) 2604, 2999
BaeI ACNNNNGTAYC 2 cut(s) 2069, 2102
BalI TGGCCA 1 cut(s) 1848
BamHI GGATCC 1 cut(s) 1328
BanI GGYRCC 1 cut(s) 3007
BanII GRGCYC 1 cut(s) 2066
Bbv12I GWGCWC 5 cut(s) 407, 434, 2604, 2711, 2999
BbvCI CCTCAGC 1 cut(s) 3003
BbvI GCAGC 3 cut(s) 840, 2079, 2616
BceAI ACGGC 2 cut(s) 2116, 2909
BciT130I CCWGG 2 cut(s) 215, 324
BciVI GTATCC 1 cut(s) 2152
BcnI CCSGG 4 cut(s) 251, 740, 1093, 1333
BcuI ACTAGT 2 cut(s) 1196, 2944
BfoI RGCGCY 1 cut(s) 1067
BfuAI ACCTGC 1 cut(s) 826
BfuI GTATCC 1 cut(s) 2152
BglII AGATCT 2 cut(s) 925, 1124
BisI GCNGC 3 cut(s) 854, 2093, 2630
BlnI CCTAGG 1 cut(s) 1175
BlpI GCTNAGC 3 cut(s) 14, 1342, 1790
BlsI GCNGC 3 cut(s) 855, 2094, 2631
BmcAI AGTACT 4 cut(s) 392, 419, 992, 2007
Bme1390I CCNGG 6 cut(s) 215, 251, 324, 740, 1093, 1333
Bme18I GGWCC 7 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546
BmeT110I CYCGRG 2 cut(s) 1678, 2556
BmgT120I GGNCC 8 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546, 2868
BmiI GGNNCC 4 cut(s) 77, 1330, 1548, 3009
BmrFI CCNGG 6 cut(s) 215, 251, 324, 740, 1093, 1333
BmrI ACTGGG 1 cut(s) 2832
BmsI GCATC 5 cut(s) 1131, 1557, 2132, 2677, 2907
BmtI GCTAGC 1 cut(s) 2622
BmuI ACTGGG 1 cut(s) 2832
BoxI GACNNNNGTC 1 cut(s) 261
BpmI CTGGAG 3 cut(s) 716, 884, 1536
Bpu10I CCTNAGC 1 cut(s) 3003
Bpu1102I GCTNAGC 3 cut(s) 14, 1342, 1790
BpuEI CTTGAG 1 cut(s) 280
BpuMI CCSGG 4 cut(s) 251, 740, 1093, 1333
Bsa29I ATCGAT 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 1713
BsaHI GRCGYC 1 cut(s) 491
BsaJI CCNNGG 8 cut(s) 55, 1175, 1332, 1692, 1879, 2147, 2278, 3070
BsaWI WCCGGW 3 cut(s) 167, 584, 958
BsaXI ACNNNNNCTCC 4 cut(s) 566, 596, 689, 719
Bsc4I CCNNNNNNNGG 8 cut(s) 99, 511, 1040, 1066, 1559, 1844, 2750, 2809
Bse118I RCCGGY 5 cut(s) 958, 1277, 1625, 2109, 2920
Bse1I ACTGG 8 cut(s) 142, 379, 388, 1954, 2184, 2383, 2820, 2827
Bse21I CCTNAGG 1 cut(s) 1111
Bse3DI GCAATG 4 cut(s) 124, 1353, 2629, 2814
BseBI CCWGG 2 cut(s) 215, 324
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 8 cut(s) 55, 1175, 1332, 1692, 1879, 2147, 2278, 3070
BseGI GGATG 3 cut(s) 1318, 2647, 2845
BseLI CCNNNNNNNGG 8 cut(s) 99, 511, 1040, 1066, 1559, 1844, 2750, 2809
BseMI GCAATG 4 cut(s) 124, 1353, 2629, 2814
BseNI ACTGG 8 cut(s) 142, 379, 388, 1954, 2184, 2383, 2820, 2827
BseRI GAGGAG 5 cut(s) 816, 2842, 2859, 2864, 3084
BseSI GKGCMC 2 cut(s) 2604, 2999
BseXI GCAGC 3 cut(s) 840, 2079, 2616
BseYI CCCAGC 1 cut(s) 2065
BsgI GTGCAG 1 cut(s) 839
BshFI GGCC 7 cut(s) 60, 103, 1455, 1848, 2219, 2398, 2870
BshNI GGYRCC 1 cut(s) 3007
BshTI ACCGGT 1 cut(s) 958
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 5 cut(s) 407, 434, 2604, 2711, 2999
BsiHKCI CYCGRG 2 cut(s) 1678, 2556
BslFI GGGAC 2 cut(s) 1282, 1532
BslI CCNNNNNNNGG 8 cut(s) 99, 511, 1040, 1066, 1559, 1844, 2750, 2809
BsmFI GGGAC 2 cut(s) 1282, 1532
BsnI GGCC 7 cut(s) 60, 103, 1455, 1848, 2219, 2398, 2870
BsoBI CYCGRG 2 cut(s) 1678, 2556
Bsp1286I GDGCHC 6 cut(s) 407, 434, 2066, 2604, 2711, 2999
Bsp1407I TGTACA 3 cut(s) 1392, 1513, 2787
Bsp143I GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
Bsp1720I GCTNAGC 3 cut(s) 14, 1342, 1790
Bsp19I CCATGG 1 cut(s) 1692
BspACI CCGC 1 cut(s) 1104
BspANI GGCC 7 cut(s) 60, 103, 1455, 1848, 2219, 2398, 2870
BspDI ATCGAT 1 cut(s) 144
BspHI TCATGA 1 cut(s) 2434
BspLI GGNNCC 4 cut(s) 77, 1330, 1548, 3009
BspMI ACCTGC 1 cut(s) 826
BspOI GCTAGC 1 cut(s) 2622
BspPI GGATC 5 cut(s) 730, 1323, 1336, 1440, 2252
BspQI GCTCTTC 1 cut(s) 2054
BspT107I GGYRCC 1 cut(s) 3007
BsrDI GCAATG 4 cut(s) 124, 1353, 2629, 2814
BsrFI RCCGGY 5 cut(s) 958, 1277, 1625, 2109, 2920
BsrGI TGTACA 3 cut(s) 1392, 1513, 2787
BsrI ACTGG 8 cut(s) 142, 379, 388, 1954, 2184, 2383, 2820, 2827
BssAI RCCGGY 5 cut(s) 958, 1277, 1625, 2109, 2920
BssECI CCNNGG 8 cut(s) 55, 1175, 1332, 1692, 1879, 2147, 2278, 3070
BssMI GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
BssNI GRCGYC 1 cut(s) 491
BssT1I CCWWGG 7 cut(s) 55, 1175, 1692, 1879, 2147, 2278, 3070
Bst2UI CCWGG 2 cut(s) 215, 324
Bst6I CTCTTC 5 cut(s) 1346, 1406, 2054, 2143, 2955
BstACI GRCGYC 1 cut(s) 491
BstAPI GCANNNNNTGC 1 cut(s) 2994
BstAUI TGTACA 3 cut(s) 1392, 1513, 2787
BstBAI YACGTR 1 cut(s) 1713
BstC8I GCNNGC 5 cut(s) 1146, 1968, 2620, 2673, 2766
BstDSI CCRYGG 1 cut(s) 1692
BstEII GGTNACC 1 cut(s) 1883
BstF5I GGATG 3 cut(s) 1318, 2647, 2845
BstH2I RGCGCY 1 cut(s) 1067
BstHHI GCGC 3 cut(s) 1066, 1572, 2526
BstKTI GATC 8 cut(s) 148, 738, 928, 1127, 1320, 1331, 1435, 2247
BstMBI GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
BstMWI GCNNNNNNNGC 7 cut(s) 1063, 1481, 1776, 1972, 2761, 2926, 2994
BstNI CCWGG 2 cut(s) 215, 324
BstNSI RCATGY 1 cut(s) 2204
BstPAI GACNNNNGTC 1 cut(s) 261
BstPI GGTNACC 1 cut(s) 1883
BstSCI CCNGG 6 cut(s) 213, 249, 322, 738, 1091, 1331
BstSLI GKGCMC 2 cut(s) 2604, 2999
BstV1I GCAGC 3 cut(s) 840, 2079, 2616
BstX2I RGATCY 3 cut(s) 925, 1124, 1328
BstYI RGATCY 3 cut(s) 925, 1124, 1328
Bsu15I ATCGAT 1 cut(s) 144
Bsu36I CCTNAGG 1 cut(s) 1111
BsuI GTATCC 1 cut(s) 2152
BsuRI GGCC 7 cut(s) 60, 103, 1455, 1848, 2219, 2398, 2870
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 1692
BtgZI GCGATG 1 cut(s) 2796
BtsCI GGATG 3 cut(s) 1318, 2647, 2845
BtsI GCAGTG 3 cut(s) 1493, 2316, 2841
BtsIMutI CAGTG 6 cut(s) 514, 1024, 1493, 2277, 2316, 2841
BveI ACCTGC 1 cut(s) 826
Cac8I GCNNGC 5 cut(s) 1146, 1968, 2620, 2673, 2766
CciI TCATGA 1 cut(s) 2434
CfoI GCGC 3 cut(s) 1066, 1572, 2526
Cfr10I RCCGGY 5 cut(s) 958, 1277, 1625, 2109, 2920
Cfr13I GGNCC 8 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546, 2868
ClaI ATCGAT 1 cut(s) 144
CseI GACGC 1 cut(s) 480
CspAI ACCGGT 1 cut(s) 958
CspCI CAANNNNNGTGG 4 cut(s) 427, 462, 2532, 2567
DpnI GATC 8 cut(s) 147, 737, 927, 1126, 1319, 1330, 1434, 2246
DpnII GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
EaeI YGGCCR 1 cut(s) 1846
Eam1104I CTCTTC 5 cut(s) 1346, 1406, 2054, 2143, 2955
EarI CTCTTC 5 cut(s) 1346, 1406, 2054, 2143, 2955
Eco130I CCWWGG 7 cut(s) 55, 1175, 1692, 1879, 2147, 2278, 3070
Eco24I GRGCYC 1 cut(s) 2066
Eco32I GATATC 1 cut(s) 1306
Eco47I GGWCC 7 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546
Eco47III AGCGCT 1 cut(s) 1065
Eco81I CCTNAGG 1 cut(s) 1111
Eco88I CYCGRG 2 cut(s) 1678, 2556
Eco91I GGTNACC 1 cut(s) 1883
EcoO109I RGGNCCY 2 cut(s) 950, 1546
EcoO65I GGTNACC 1 cut(s) 1883
EcoRI GAATTC 2 cut(s) 894, 2351
EcoRII CCWGG 2 cut(s) 213, 322
EcoRV GATATC 1 cut(s) 1306
EcoT14I CCWWGG 7 cut(s) 55, 1175, 1692, 1879, 2147, 2278, 3070
EcoT38I GRGCYC 1 cut(s) 2066
ErhI CCWWGG 7 cut(s) 55, 1175, 1692, 1879, 2147, 2278, 3070
FalI AAGNNNNNCTT 4 cut(s) 15, 47, 303, 335
FaqI GGGAC 2 cut(s) 1282, 1532
FauI CCCGC 1 cut(s) 1111
FauNDI CATATG 1 cut(s) 1466
Fnu4HI GCNGC 3 cut(s) 854, 2093, 2630
FokI GGATG 3 cut(s) 1325, 2654, 2852
FriOI GRGCYC 1 cut(s) 2066
Fsp4HI GCNGC 3 cut(s) 854, 2093, 2630
FspI TGCGCA 1 cut(s) 1571
GlaI GCGC 3 cut(s) 1065, 1571, 2525
GluI GCNGC 3 cut(s) 854, 2093, 2630
GsaI CCCAGC 1 cut(s) 2069
GsuI CTGGAG 3 cut(s) 716, 884, 1536
HaeII RGCGCY 1 cut(s) 1067
HaeIII GGCC 7 cut(s) 60, 103, 1455, 1848, 2219, 2398, 2870
HgaI GACGC 1 cut(s) 480
HhaI GCGC 3 cut(s) 1066, 1572, 2526
Hin1I GRCGYC 1 cut(s) 491
Hin6I GCGC 3 cut(s) 1064, 1570, 2524
HinP1I GCGC 3 cut(s) 1064, 1570, 2524
HindIII AAGCTT 5 cut(s) 24, 982, 1768, 1973, 2318
Hpy166II GTNNAC 7 cut(s) 52, 1393, 1395, 2275, 2602, 2788, 2997
Hpy8I GTNNAC 7 cut(s) 52, 1393, 1395, 2275, 2602, 2788, 2997
HpyCH4IV ACGT 2 cut(s) 1712, 1859
HpyF10VI GCNNNNNNNGC 7 cut(s) 1063, 1481, 1776, 1972, 2761, 2926, 2994
HpySE526I ACGT 2 cut(s) 1712, 1859
Hsp92I GRCGYC 1 cut(s) 491
HspAI GCGC 3 cut(s) 1064, 1570, 2524
Kzo9I GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
LguI GCTCTTC 1 cut(s) 2054
LmnI GCTCC 3 cut(s) 242, 1062, 2481
Lsp1109I GCAGC 3 cut(s) 840, 2079, 2616
LweI GCATC 5 cut(s) 1131, 1557, 2132, 2677, 2907
MaeII ACGT 2 cut(s) 1712, 1859
MalI GATC 8 cut(s) 147, 737, 927, 1126, 1319, 1330, 1434, 2246
MboI GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
MfeI CAATTG 2 cut(s) 1850, 2544
MflI RGATCY 3 cut(s) 925, 1124, 1328
MhlI GDGCHC 6 cut(s) 407, 434, 2066, 2604, 2711, 2999
MlsI TGGCCA 1 cut(s) 1848
MluNI TGGCCA 1 cut(s) 1848
MlyI GAGTC 7 cut(s) 271, 700, 707, 1880, 1913, 2037, 2808
MmeI TCCRAC 5 cut(s) 249, 442, 596, 1245, 1307
Mox20I TGGCCA 1 cut(s) 1848
MroXI GAANNNNTTC 3 cut(s) 578, 685, 1673
MscI TGGCCA 1 cut(s) 1848
MseI TTAA 6 cut(s) 21, 831, 1443, 1607, 1656, 1772
MslI CAYNNNNRTG 3 cut(s) 507, 2607, 2714
Msp20I TGGCCA 1 cut(s) 1848
MspA1I CMGCKG 1 cut(s) 3002
MspR9I CCNGG 6 cut(s) 215, 251, 324, 740, 1093, 1333
MunI CAATTG 2 cut(s) 1850, 2544
MvaI CCWGG 2 cut(s) 215, 324
MwoI GCNNNNNNNGC 7 cut(s) 1063, 1481, 1776, 1972, 2761, 2926, 2994
NciI CCSGG 4 cut(s) 251, 740, 1093, 1333
NcoI CCATGG 1 cut(s) 1692
NdeI CATATG 1 cut(s) 1466
NdeII GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
NheI GCTAGC 1 cut(s) 2618
NlaIV GGNNCC 4 cut(s) 77, 1330, 1548, 3009
NmuCI GTSAC 6 cut(s) 122, 294, 567, 1883, 2501, 2656
NsbI TGCGCA 1 cut(s) 1571
NspI RCATGY 1 cut(s) 2204
OliI CACNNNNGTG 2 cut(s) 507, 2714
PaeR7I CTCGAG 1 cut(s) 2556
PagI TCATGA 1 cut(s) 2434
PaqCI CACCTGC 1 cut(s) 826
PciSI GCTCTTC 1 cut(s) 2054
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdmI GAANNNNTTC 3 cut(s) 578, 685, 1673
PflMI CCANNNNNTGG 4 cut(s) 1040, 1066, 1559, 2750
PfoI TCCNGGA 3 cut(s) 249, 322, 738
PinAI ACCGGT 1 cut(s) 958
PkrI GCNGC 3 cut(s) 855, 2094, 2631
PleI GAGTC 7 cut(s) 270, 699, 706, 1879, 1913, 2036, 2807
PpsI GAGTC 7 cut(s) 270, 699, 706, 1879, 1913, 2036, 2807
Ppu21I YACGTR 1 cut(s) 1713
PpuMI RGGWCCY 2 cut(s) 950, 1546
PshAI GACNNNNGTC 1 cut(s) 261
Psp1406I AACGTT 1 cut(s) 1859
Psp5II RGGWCCY 2 cut(s) 950, 1546
Psp6I CCWGG 2 cut(s) 213, 322
PspEI GGTNACC 1 cut(s) 1883
PspFI CCCAGC 1 cut(s) 2065
PspGI CCWGG 2 cut(s) 213, 322
PspN4I GGNNCC 4 cut(s) 77, 1330, 1548, 3009
PspPI GGNCC 8 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546, 2868
PspPPI RGGWCCY 2 cut(s) 950, 1546
PspXI VCTCGAGB 1 cut(s) 2556
PsuI RGATCY 3 cut(s) 925, 1124, 1328
PvuII CAGCTG 1 cut(s) 3002
RseI CAYNNNNRTG 3 cut(s) 507, 2607, 2714
SapI GCTCTTC 1 cut(s) 2054
SaqAI TTAA 6 cut(s) 21, 831, 1443, 1607, 1656, 1772
SatI GCNGC 3 cut(s) 854, 2093, 2630
Sau3AI GATC 8 cut(s) 145, 735, 925, 1124, 1317, 1328, 1432, 2244
Sau96I GGNCC 8 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546, 2868
ScaI AGTACT 4 cut(s) 392, 419, 992, 2007
SchI GAGTC 7 cut(s) 271, 700, 707, 1880, 1913, 2037, 2808
ScrFI CCNGG 6 cut(s) 215, 251, 324, 740, 1093, 1333
SduI GDGCHC 6 cut(s) 407, 434, 2066, 2604, 2711, 2999
SfaNI GCATC 5 cut(s) 1131, 1557, 2132, 2677, 2907
Sfr274I CTCGAG 1 cut(s) 2556
SinI GGWCC 7 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546
SlaI CTCGAG 1 cut(s) 2556
SmiMI CAYNNNNRTG 3 cut(s) 507, 2607, 2714
SmlI CTYRAG 2 cut(s) 259, 2556
SmoI CTYRAG 2 cut(s) 259, 2556
SpeI ACTAGT 2 cut(s) 1196, 2944
SsiI CCGC 1 cut(s) 1104
SspI AATATT 3 cut(s) 1264, 1804, 2454
StyD4I CCNGG 6 cut(s) 213, 249, 322, 738, 1091, 1331
StyI CCWWGG 7 cut(s) 55, 1175, 1692, 1879, 2147, 2278, 3070
TaiI ACGT 2 cut(s) 1715, 1862
TaqI TCGA 6 cut(s) 144, 407, 689, 1420, 2176, 2557
TatI WGTACW 9 cut(s) 390, 417, 990, 1193, 1392, 1513, 2005, 2466, 2787
Tru1I TTAA 6 cut(s) 21, 831, 1443, 1607, 1656, 1772
Tru9I TTAA 6 cut(s) 21, 831, 1443, 1607, 1656, 1772
TscAI CASTG 6 cut(s) 514, 1024, 1500, 2277, 2316, 2841
TseFI GTSAC 6 cut(s) 122, 294, 567, 1883, 2501, 2656
TseI GCWGC 3 cut(s) 853, 2092, 2629
Tsp45I GTSAC 6 cut(s) 122, 294, 567, 1883, 2501, 2656
TspGWI ACGGA 2 cut(s) 494, 2545
TspRI CASTG 6 cut(s) 514, 1024, 1500, 2277, 2316, 2841
Van91I CCANNNNNTGG 4 cut(s) 1040, 1066, 1559, 2750
VneI GTGCAC 2 cut(s) 2600, 2995
VpaK11BI GGWCC 7 cut(s) 211, 562, 950, 1069, 1166, 1280, 1546
XbaI TCTAGA 3 cut(s) 307, 728, 3017
XceI RCATGY 1 cut(s) 2204
XcmI CCANNNNNNNNNTGG 1 cut(s) 2878
XhoI CTCGAG 1 cut(s) 2556
XmaJI CCTAGG 1 cut(s) 1175
XmnI GAANNNNTTC 3 cut(s) 578, 685, 1673
ZrmI AGTACT 4 cut(s) 392, 419, 992, 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.