AT1G74360

LRR receptor-like serine threonine-protein kinase At1g74360

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004694
Physical Location & Seq
Forward (+)
27954129 .. 27958114
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74360.1

Sequence Viewer

Length: 3321 bp
ATGACTATGGTGACGCGTGTGATCATGACGGATGATGATTCTCAGTCACTTTGCTTTCTTTGTTTCTTACTCTTCTTTTTCATCACTGCTATAGCAGTGGCTGGAGATTCTCTGGACAGTGATAGAGAAGTCTTGTTAAGCCTAAAATCGTACCTTGAATCACGGAACCCACAGAACCGAGGACTATACACGGAATGGAAAATGGAGAACCAAGATGTTGTGTGTCAATGGCCTGGAATCATATGTACACCTCAGAGAAGTAGAGTTACAGGGATCAATCTAACTGATTCCACCATTTCAGGTCCTCTATTCAAGAACTTCTCCGCTTTAACGGAGCTCACATATCTCGATTTATCAAGGAATACGATCGAAGGGGAGATTCCAGATGACTTGAGCCGTTGTCACAACTTAAAGCATCTGAATCTTTCTCATAATATCCTTGAAGGAGAGCTAAGTTTACCCGGGTTGTCAAATCTTGAGGTTCTTGATCTTTCGTTGAATAGGATTACGGGTGATATTCAGTCTAGCTTCCCATTGTTCTGCAACAGTTTGGTTGTTGCAAATCTGTCGACCAACAACTTTACTGGAAGAATCGATGACATCTTCAACGGGTGTAGGAATCTCAAGTATGTTGACTTCAGTTCCAATAGATTCAGTGGAGAAGTGTGGACTGGTTTTGGGAGGCTGGTCGAGTTTTCAGTTGCTGATAATCATCTCTCCGGGAATATCTCTGCTTCAATGTTCAGGGGGAACTGTACTTTGCAAATGTTGGATTTGTCTGGGAACGCTTTTGGTGGGGAATTTCCAGGCCAAGTTTCGAATTGCCAGAATCTAAATGTACTGAATCTTTGGGGAAACAAGTTCACAGGGAACATTCCAGCTGAGATTGGTTCCATATCCTCTCTCAAAGGTTTGTACTTGGGGAATAATACATTTTCTCGAGACATACCGGAAACTCTCCTGAACTTGACCAACTTGGTGTTTCTGGATTTGAGCAGAAACAAATTTGGAGGAGACATTCAAGAAATCTTTGGGAGATTCACGCAAGTAAAGTATCTAGTCTTGCATGCAAACTCATACGTAGGAGGCATCAATTCTTCCAACATCCTCAAGTTACCCAATCTTTCAAGGCTAGATTTGGGCTACAACAATTTCTCGGGACAGTTACCTACTGAAATTTCTCAGATACAGAGTTTGAAGTTCTTGATTCTTGCTTATAATAACTTCAGTGGCGATATACCACAGGAGTATGGGAACATGCCGGGGCTTCAAGCACTTGATCTCTCCTTTAACAAGCTGACCGGTTCGATACCAGCTTCATTTGGGAAATTGACCTCTCTTTTGTGGCTAATGCTTGCAAACAACTCTCTATCAGGAGAAATCCCTCGAGAGATTGGCAACTGCACAAGCCTTTTGTGGTTTAACGTGGCAAACAACCAGCTCTCTGGTAGATTCCATCCTGAATTGACTAGAATGGGGAGTAATCCTTCTCCAACATTTGAAGTGAATAGGCAAAACAAAGACAAGATAATTGCTGGTTCTGGTGAATGCTTGGCGATGAAGAGATGGATTCCAGCGGAGTTCCCTCCGTTCAACTTTGTATACGCAATTCTAACCAAAAAGAGTTGCAGAAGCCTATGGGACCATGTTCTCAAAGGGTACGGTCTCTTTCCTGTCTGTTCTGCTGGTTCCACGGTACGCACGCTTAAAATTTCAGCCTATCTTCAGCTCAGTGGAAATAAGTTTTCAGGTGAGATTCCTGCAAGCATCTCTCAGATGGACAGGCTGAGCACACTACATTTGGGTTTCAATGAGTTTGAGGGGAAACTGCCTCCAGAGATAGGACAATTGCCTCTTGCATTCCTCAACCTTACTCGAAACAATTTCTCGGGCGAGATTCCTCAAGAAATCGGAAATCTGAAGTGCCTGCAGAATCTTGATCTGTCTTTCAACAATTTCTCTGGAAACTTTCCAACGAGTTTGAATGACTTGAATGAGCTGAGTAAGTTCAACATCTCATATAACCCTTTCATTTCTGGCGCGATACCAACAACAGGACAAGTAGCAACCTTTGACAAAGACTCCTTTCTTGGAAATCCGCTGCTGCGGTTTCCTAGTTTCTTCAACCAATCAGGGAACAACACGAGGAAGATTTCCAACCAAGTCCTAGGAAACAGGCCAAGAACTCTTCTTTTGATTTGGATTTCCTTGGCTCTTGCATTGGCGTTTATTGCATGTTTAGTGGTATCAGGTATTGTACTTATGGTTGTTAAGGCTTCAAGGGAAGCAGAAATCGATCTCTTAGATGGGTCGAAAACCAGACATGACATGACTTCCAGTTCAGGTGGATCATCGCCATGGCTATCAGGAAAAATCAAGGTCATTCGCTTAGACAAATCAACTTTCACATACGCTGACATTCTGAAAGCAACCAGTAACTTCTCTGAGGAGAGAGTGGTAGGTAGGGGAGGCTATGGAACTGTTTACAGAGGCGTATTGCCTGATGGAAGAGAAGTTGCAGTCAAGAAGCTTCAGAGGGAAGGCACAGAAGCAGAGAAAGAGTTCAGAGCTGAAATGGAAGTTCTAAGTGCCAACGCATTTGGAGATTGGGCACATCCGAACCTCGTGAGGCTATATGGATGGTGCCTAGATGGGTCAGAGAAAATCTTGGTGCACGAATACATGGGAGGCGGGAGCTTAGAGGAGCTCATCACAGACAAAACAAAACTTCAATGGAAGAAACGTATTGATATAGCCACAGATGTAGCAAGGGGATTAGTGTTTTTACACCACGAATGCTACCCTTCCATCGTTCACAGAGACGTCAAAGCCAGTAACGTTCTTTTGGATAAACACGGGAATGCGAGAGTGACGGATTTTGGACTAGCAAGACTCTTAAATGTAGGAGATAGCCATGTGAGCACAGTGATTGCAGGCACAATCGGGTATGTAGCTCCCGAGTATGGACAAACTTGGCAAGCCACCACAAGAGGAGATGTTTACAGCTACGGAGTATTGACCATGGAACTCGCGACGGGTAGAAGAGCTGTTGATGGAGGAGAAGAGTGTTTGGTCGAATGGGCAAGACGTGTAATGACAGGTAACATGACAGCCAAAGGTTCACCAATAACCCTGTCAGGGACCAAACCAGGGAACGGAGCTGAGCAAATGACTGAGCTACTAAAGATTGGTGTGAAGTGTACAGCTGATCATCCTCAGGCAAGACCAAACATGAAAGAAGTTCTAGCTATGTTGGTTAAGATATCCGGTAAAGCAGAGCTCTTCAATGGCTTATCTTCACAAGGTTACATAGAAATGTAA

Protein Analysis

1106

Amino Acids

121.89

Weight (kDa)

6.43

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 105 - 145 4.5e-07 Leucine rich repeat
LRR_8 PF13855 277 - 336 4.9e-06 Leucine rich repeat
LRR_8 PF13855 349 - 409 5.5e-06 Leucine rich repeat
LRR_14 PF23598 391 - 477 1e-07 Leucine-rich repeat region
LRR_8 PF13855 421 - 481 6.9e-08 Leucine rich repeat
LRR_14 PF23598 574 - 674 1.8e-08 Leucine-rich repeat region
Pkinase PF00069 815 - 1083 2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 817 - 1084 4.8e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1218
AatII GACGTC 1 cut(s) 2826
AccB1I GGYRCC 1 cut(s) 2643
AccI GTMKAC 2 cut(s) 569, 1602
AccII CGCG 3 cut(s) 16, 2042, 3032
AciI CCGC 5 cut(s) 324, 1577, 2099, 2107, 2691
AclI AACGTT 1 cut(s) 2838
AclWI GGATC 2 cut(s) 281, 2356
AcsI RAATTY 4 cut(s) 800, 1004, 1176, 1710
AcuI CTGAAG 5 cut(s) 622, 1210, 1709, 1940, 2516
AcyI GRCGYC 1 cut(s) 2823
AfaI GTAC 9 cut(s) 152, 247, 757, 840, 917, 1661, 1698, 2259, 3202
AfiI CCNNNNNNNGG 6 cut(s) 1841, 2054, 2963, 3138, 3150, 3155
AflIII ACRYGT 2 cut(s) 14, 3088
AgeI ACCGGT 1 cut(s) 1301
AjiI CACGTC 1 cut(s) 3089
AjnI CCWGG 3 cut(s) 232, 805, 3148
Alw21I GWGCWC 6 cut(s) 339, 1793, 2676, 2709, 2924, 3282
Alw26I GTCTC 4 cut(s) 936, 1008, 1670, 2814
Alw44I GTGCAC 1 cut(s) 2672
AlwI GGATC 2 cut(s) 281, 2356
AlwNI CAGNNNCTG 2 cut(s) 101, 704
Ama87I CYCGRG 6 cut(s) 461, 939, 1156, 1386, 1888, 2957
AoxI GGCC 3 cut(s) 230, 808, 2177
ApaLI GTGCAC 1 cut(s) 2672
ApeKI GCWGC 2 cut(s) 2101, 2104
ApoI RAATTY 4 cut(s) 800, 1004, 1176, 1710
ArsI GACNNNNNNTTYG 2 cut(s) 1324, 1356
AsiGI ACCGGT 1 cut(s) 1301
Asp700I GAANNNNTTC 1 cut(s) 2561
AspA2I CCTAGG 1 cut(s) 2167
AspLEI GCGC 1 cut(s) 2042
AspS9I GGNCC 3 cut(s) 302, 1642, 3141
AsuC2I CCSGG 4 cut(s) 462, 463, 721, 1263
AsuHPI GGTGA 5 cut(s) 22, 524, 1556, 1763, 3114
AsuII TTCGAA 1 cut(s) 818
AvaI CYCGRG 6 cut(s) 461, 939, 1156, 1386, 1888, 2957
AvaII GGWCC 3 cut(s) 302, 1642, 3141
AvrII CCTAGG 1 cut(s) 2167
AxyI CCTNAGG 1 cut(s) 3216
BaeGI GKGCMC 2 cut(s) 2614, 2676
BanI GGYRCC 1 cut(s) 2643
BanII GRGCYC 3 cut(s) 339, 2709, 3282
BarI GAAGNNNNNNTAC 4 cut(s) 250, 282, 620, 652
BauI CACGAG 2 cut(s) 2143, 2624
Bbv12I GWGCWC 6 cut(s) 339, 1793, 2676, 2709, 2924, 3282
BbvI GCAGC 2 cut(s) 2088, 2091
BccI CCATC 9 cut(s) 1464, 1560, 1771, 2300, 2498, 2634, 2645, 2816, 3047
BceAI ACGGC 1 cut(s) 381
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 3 cut(s) 234, 807, 3150
BclI TGATCA 2 cut(s) 21, 3208
BcnI CCSGG 4 cut(s) 462, 463, 721, 1263
BcoDI GTCTC 4 cut(s) 936, 1008, 1670, 2814
BfaI CTAG 9 cut(s) 525, 1058, 1133, 1470, 2115, 2168, 2648, 2885, 3245
BfmI CTRYAG 2 cut(s) 90, 1928
BisI GCNGC 2 cut(s) 2102, 2105
BlnI CCTAGG 1 cut(s) 2167
BlpI GCTNAGC 2 cut(s) 1787, 3162
BlsI GCNGC 2 cut(s) 2103, 2106
Bme1390I CCNGG 7 cut(s) 234, 462, 463, 721, 807, 1263, 3150
Bme18I GGWCC 3 cut(s) 302, 1642, 3141
BmeT110I CYCGRG 6 cut(s) 461, 939, 1156, 1386, 1888, 2957
BmgBI CACGTC 1 cut(s) 3089
BmgT120I GGNCC 3 cut(s) 302, 1642, 3141
BmiI GGNNCC 6 cut(s) 167, 892, 1643, 1690, 2645, 3142
BmrFI CCNGG 7 cut(s) 234, 462, 463, 721, 807, 1263, 3150
BmsI GCATC 3 cut(s) 424, 1098, 1776
BpmI CTGGAG 2 cut(s) 123, 1818
Bpu1102I GCTNAGC 2 cut(s) 1787, 3162
Bpu14I TTCGAA 1 cut(s) 818
BpuEI CTTGAG 5 cut(s) 412, 497, 608, 1094, 1887
BpuMI CCSGG 4 cut(s) 462, 463, 721, 1263
Bsa29I ATCGAT 2 cut(s) 594, 2295
BsaAI YACGTR 1 cut(s) 1081
BsaBI GATNNNNATC 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 2823
BsaI GGTCTC 1 cut(s) 1670
BsaJI CCNNGG 9 cut(s) 178, 461, 1262, 1692, 2167, 2208, 2357, 3021, 3149
BsaWI WCCGGW 3 cut(s) 949, 1301, 3266
BsaXI ACNNNNNCTCC 2 cut(s) 2066, 2096
Bsc4I CCNNNNNNNGG 6 cut(s) 1841, 2054, 2963, 3138, 3150, 3155
Bse118I RCCGGY 1 cut(s) 1301
Bse1I ACTGG 5 cut(s) 589, 676, 2337, 2433, 2832
Bse21I CCTNAGG 1 cut(s) 3216
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 3 cut(s) 234, 807, 3150
BseCI ATCGAT 2 cut(s) 594, 2295
BseDI CCNNGG 9 cut(s) 178, 461, 1262, 1692, 2167, 2208, 2357, 3021, 3149
BseGI GGATG 6 cut(s) 37, 1104, 1456, 2614, 2645, 3211
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 6 cut(s) 1841, 2054, 2963, 3138, 3150, 3155
BseNI ACTGG 5 cut(s) 589, 676, 2337, 2433, 2832
BseRI GAGGAG 5 cut(s) 1026, 2462, 2717, 3006, 3072
BseSI GKGCMC 2 cut(s) 2614, 2676
BseXI GCAGC 2 cut(s) 2088, 2091
BsgI GTGCAG 1 cut(s) 1387
Bsh1236I CGCG 3 cut(s) 16, 2042, 3032
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 3 cut(s) 232, 810, 2179
BshNI GGYRCC 1 cut(s) 2643
BshTI ACCGGT 1 cut(s) 1301
BshVI ATCGAT 2 cut(s) 594, 2295
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 6 cut(s) 339, 1793, 2676, 2709, 2924, 3282
BsiHKCI CYCGRG 6 cut(s) 461, 939, 1156, 1386, 1888, 2957
BsiSI CCGG 6 cut(s) 462, 720, 950, 1262, 1302, 3267
BslFI GGGAC 3 cut(s) 1173, 1655, 3154
BslI CCNNNNNNNGG 6 cut(s) 1841, 2054, 2963, 3138, 3150, 3155
BsmAI GTCTC 4 cut(s) 936, 1008, 1670, 2814
BsmBI CGTCTC 1 cut(s) 2814
BsmFI GGGAC 3 cut(s) 1173, 1655, 3154
BsmI GAATGC 4 cut(s) 1553, 1859, 2801, 2866
BsnI GGCC 3 cut(s) 232, 810, 2179
Bso31I GGTCTC 1 cut(s) 1670
BsoBI CYCGRG 6 cut(s) 461, 939, 1156, 1386, 1888, 2957
Bsp119I TTCGAA 1 cut(s) 818
Bsp1286I GDGCHC 7 cut(s) 339, 1793, 2614, 2676, 2709, 2924, 3282
Bsp1407I TGTACA 2 cut(s) 245, 3200
Bsp143I GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
Bsp1720I GCTNAGC 2 cut(s) 1787, 3162
Bsp19I CCATGG 2 cut(s) 2357, 3021
Bsp68I TCGCGA 1 cut(s) 3032
BspACI CCGC 5 cut(s) 324, 1577, 2099, 2107, 2691
BspANI GGCC 3 cut(s) 232, 810, 2179
BspDI ATCGAT 2 cut(s) 594, 2295
BspFNI CGCG 3 cut(s) 16, 2042, 3032
BspHI TCATGA 1 cut(s) 24
BspLI GGNNCC 6 cut(s) 167, 892, 1643, 1690, 2645, 3142
BspMAI CTGCAG 1 cut(s) 1932
BspPI GGATC 2 cut(s) 281, 2356
BspQI GCTCTTC 2 cut(s) 3037, 3287
BspT104I TTCGAA 1 cut(s) 818
BspT107I GGYRCC 1 cut(s) 2643
BspTNI GGTCTC 1 cut(s) 1670
BsrFI RCCGGY 1 cut(s) 1301
BsrGI TGTACA 2 cut(s) 245, 3200
BsrI ACTGG 5 cut(s) 589, 676, 2337, 2433, 2832
BssAI RCCGGY 1 cut(s) 1301
BssECI CCNNGG 9 cut(s) 178, 461, 1262, 1692, 2167, 2208, 2357, 3021, 3149
BssMI GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
BssNAI GTATAC 1 cut(s) 1603
BssNI GRCGYC 1 cut(s) 2823
BssSI CACGAG 2 cut(s) 2143, 2624
BssT1I CCWWGG 4 cut(s) 2167, 2208, 2357, 3021
Bst1107I GTATAC 1 cut(s) 1603
Bst2BI CACGAG 2 cut(s) 2143, 2624
Bst2UI CCWGG 3 cut(s) 234, 807, 3150
Bst4CI ACNGT 8 cut(s) 119, 548, 755, 1164, 1664, 1696, 2482, 2926
Bst6I CTCTTC 7 cut(s) 77, 1556, 2193, 2503, 3037, 3057, 3287
BstACI GRCGYC 1 cut(s) 2823
BstAUI TGTACA 2 cut(s) 245, 3200
BstBAI YACGTR 1 cut(s) 1081
BstBI TTCGAA 1 cut(s) 818
BstC8I GCNNGC 7 cut(s) 1068, 1356, 1703, 1765, 1928, 2935, 2979
BstDSI CCRYGG 3 cut(s) 1692, 2357, 3021
BstF5I GGATG 6 cut(s) 37, 1104, 1456, 2614, 2645, 3211
BstFNI CGCG 3 cut(s) 16, 2042, 3032
BstHHI GCGC 1 cut(s) 2042
BstKTI GATC 9 cut(s) 24, 276, 369, 490, 1282, 1942, 2299, 2351, 3211
BstMAI GTCTC 4 cut(s) 936, 1008, 1670, 2814
BstMBI GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 2 cut(s) 2231, 2919
BstNI CCWGG 3 cut(s) 234, 807, 3150
BstNSI RCATGY 3 cut(s) 1070, 1261, 2238
BstSCI CCNGG 7 cut(s) 232, 460, 461, 719, 805, 1261, 3148
BstSFI CTRYAG 2 cut(s) 90, 1928
BstSLI GKGCMC 2 cut(s) 2614, 2676
BstSNI TACGTA 1 cut(s) 1081
BstUI CGCG 3 cut(s) 16, 2042, 3032
BstV1I GCAGC 2 cut(s) 2088, 2091
BstXI CCANNNNNNTGG 1 cut(s) 1445
BstZ17I GTATAC 1 cut(s) 1603
Bsu15I ATCGAT 2 cut(s) 594, 2295
Bsu36I CCTNAGG 1 cut(s) 3216
BsuRI GGCC 3 cut(s) 232, 810, 2179
BsuTUI ATCGAT 2 cut(s) 594, 2295
BtgI CCRYGG 3 cut(s) 1692, 2357, 3021
BtgZI GCGATG 2 cut(s) 1571, 2337
BtrI CACGTC 1 cut(s) 3089
BtsCI GGATG 6 cut(s) 37, 1104, 1456, 2614, 2645, 3211
BtsI GCAGTG 2 cut(s) 84, 102
BtsIMutI CAGTG 7 cut(s) 84, 102, 124, 661, 1234, 1738, 2931
BtuMI TCGCGA 1 cut(s) 3032
Cac8I GCNNGC 7 cut(s) 1068, 1356, 1703, 1765, 1928, 2935, 2979
CaiI CAGNNNCTG 2 cut(s) 101, 704
CciI TCATGA 1 cut(s) 24
CfoI GCGC 1 cut(s) 2042
Cfr10I RCCGGY 1 cut(s) 1301
Cfr13I GGNCC 3 cut(s) 302, 1642, 3141
Cfr9I CCCGGG 1 cut(s) 461
ClaI ATCGAT 2 cut(s) 594, 2295
CseI GACGC 1 cut(s) 22
Csp6I GTAC 9 cut(s) 151, 246, 756, 839, 916, 1660, 1697, 2258, 3201
CspAI ACCGGT 1 cut(s) 1301
CviQI GTAC 9 cut(s) 151, 246, 756, 839, 916, 1660, 1697, 2258, 3201
DpnI GATC 9 cut(s) 23, 275, 368, 489, 1281, 1941, 2298, 2350, 3210
DpnII GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
Eam1104I CTCTTC 7 cut(s) 77, 1556, 2193, 2503, 3037, 3057, 3287
EarI CTCTTC 7 cut(s) 77, 1556, 2193, 2503, 3037, 3057, 3287
Ecl136II GAGCTC 3 cut(s) 337, 2707, 3280
Eco105I TACGTA 1 cut(s) 1081
Eco130I CCWWGG 4 cut(s) 2167, 2208, 2357, 3021
Eco24I GRGCYC 3 cut(s) 339, 2709, 3282
Eco31I GGTCTC 1 cut(s) 1670
Eco32I GATATC 1 cut(s) 3264
Eco47I GGWCC 3 cut(s) 302, 1642, 3141
Eco53kI GAGCTC 3 cut(s) 337, 2707, 3280
Eco57I CTGAAG 5 cut(s) 622, 1210, 1709, 1940, 2516
Eco81I CCTNAGG 1 cut(s) 3216
Eco88I CYCGRG 6 cut(s) 461, 939, 1156, 1386, 1888, 2957
EcoICRI GAGCTC 3 cut(s) 337, 2707, 3280
EcoO109I RGGNCCY 1 cut(s) 302
EcoRII CCWGG 3 cut(s) 232, 805, 3148
EcoRV GATATC 1 cut(s) 3264
EcoT14I CCWWGG 4 cut(s) 2167, 2208, 2357, 3021
EcoT38I GRGCYC 3 cut(s) 339, 2709, 3282
ErhI CCWWGG 4 cut(s) 2167, 2208, 2357, 3021
Esp3I CGTCTC 1 cut(s) 2814
FaqI GGGAC 3 cut(s) 1173, 1655, 3154
FauI CCCGC 1 cut(s) 2684
FauNDI CATATG 1 cut(s) 242
FbaI TGATCA 2 cut(s) 21, 3208
FblI GTMKAC 2 cut(s) 569, 1602
Fnu4HI GCNGC 2 cut(s) 2102, 2105
FokI GGATG 6 cut(s) 44, 1091, 1443, 2601, 2652, 3198
FriOI GRGCYC 3 cut(s) 339, 2709, 3282
Fsp4HI GCNGC 2 cut(s) 2102, 2105
FspBI CTAG 9 cut(s) 525, 1058, 1133, 1470, 2115, 2168, 2648, 2885, 3245
GlaI GCGC 1 cut(s) 2041
GluI GCNGC 2 cut(s) 2102, 2105
GsuI CTGGAG 2 cut(s) 123, 1818
HaeIII GGCC 3 cut(s) 232, 810, 2179
HapII CCGG 6 cut(s) 462, 720, 950, 1262, 1302, 3267
HgaI GACGC 1 cut(s) 22
HhaI GCGC 1 cut(s) 2042
Hin1I GRCGYC 1 cut(s) 2823
Hin6I GCGC 1 cut(s) 2040
HinP1I GCGC 1 cut(s) 2040
HincII GTYRAC 2 cut(s) 570, 634
HindII GTYRAC 2 cut(s) 570, 634
HindIII AAGCTT 1 cut(s) 2528
HpaII CCGG 6 cut(s) 462, 720, 950, 1262, 1302, 3267
HphI GGTGA 5 cut(s) 22, 524, 1556, 1763, 3114
Hpy99I CGWCG 1 cut(s) 3037
HpyAV CCTTC 5 cut(s) 365, 437, 1497, 2534, 2814
HpyCH4III ACNGT 8 cut(s) 119, 548, 755, 1164, 1664, 1696, 2482, 2926
HpyCH4IV ACGT 6 cut(s) 1080, 1425, 2743, 2823, 2838, 3088
HpyF10VI GCNNNNNNNGC 2 cut(s) 2231, 2919
HpySE526I ACGT 6 cut(s) 1080, 1425, 2743, 2823, 2838, 3088
Hsp92I GRCGYC 1 cut(s) 2823
HspAI GCGC 1 cut(s) 2040
Ksp22I TGATCA 2 cut(s) 21, 3208
Kzo9I GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
LguI GCTCTTC 2 cut(s) 3037, 3287
LmnI GCTCC 5 cut(s) 334, 2694, 2704, 2959, 3158
Lsp1109I GCAGC 2 cut(s) 2088, 2091
LweI GCATC 3 cut(s) 424, 1098, 1776
MaeI CTAG 9 cut(s) 525, 1058, 1133, 1470, 2115, 2168, 2648, 2885, 3245
MaeII ACGT 6 cut(s) 1080, 1425, 2743, 2823, 2838, 3088
MalI GATC 9 cut(s) 23, 275, 368, 489, 1281, 1941, 2298, 2350, 3210
MboI GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
MfeI CAATTG 1 cut(s) 1847
MhlI GDGCHC 7 cut(s) 339, 1793, 2614, 2676, 2709, 2924, 3282
MluI ACGCGT 1 cut(s) 14
MlyI GAGTC 2 cut(s) 2075, 2886
MmeI TCCRAC 5 cut(s) 750, 1125, 1517, 1997, 2181
MroXI GAANNNNTTC 1 cut(s) 2561
MseI TTAA 9 cut(s) 137, 329, 410, 1290, 1422, 1707, 2271, 2897, 3258
MslI CAYNNNNRTG 3 cut(s) 2356, 2859, 3314
MspA1I CMGCKG 4 cut(s) 881, 1577, 2101, 3206
MspI CCGG 6 cut(s) 462, 720, 950, 1262, 1302, 3267
MspR9I CCNGG 7 cut(s) 234, 462, 463, 721, 807, 1263, 3150
MunI CAATTG 1 cut(s) 1847
Mva1269I GAATGC 4 cut(s) 1553, 1859, 2801, 2866
MvaI CCWGG 3 cut(s) 234, 807, 3150
MvnI CGCG 3 cut(s) 16, 2042, 3032
MwoI GCNNNNNNNGC 2 cut(s) 2231, 2919
NciI CCSGG 4 cut(s) 462, 463, 721, 1263
NcoI CCATGG 2 cut(s) 2357, 3021
NdeI CATATG 1 cut(s) 242
NdeII GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
NlaIV GGNNCC 6 cut(s) 167, 892, 1643, 1690, 2645, 3142
NmuCI GTSAC 4 cut(s) 10, 45, 401, 2869
NruI TCGCGA 1 cut(s) 3032
NspI RCATGY 3 cut(s) 1070, 1261, 2238
NspV TTCGAA 1 cut(s) 818
PaeI GCATGC 1 cut(s) 1070
PaeR7I CTCGAG 2 cut(s) 939, 1386
PagI TCATGA 1 cut(s) 24
PciSI GCTCTTC 2 cut(s) 3037, 3287
PctI GAATGC 4 cut(s) 1553, 1859, 2801, 2866
PdmI GAANNNNTTC 1 cut(s) 2561
PfoI TCCNGGA 1 cut(s) 719
PinAI ACCGGT 1 cut(s) 1301
PkrI GCNGC 2 cut(s) 2103, 2106
Ple19I CGATCG 1 cut(s) 369
PleI GAGTC 2 cut(s) 2075, 2886
PpsI GAGTC 2 cut(s) 2075, 2886
Ppu21I YACGTR 1 cut(s) 1081
PpuMI RGGWCCY 1 cut(s) 302
PsiI TTATAA 1 cut(s) 1218
Psp124BI GAGCTC 3 cut(s) 339, 2709, 3282
Psp1406I AACGTT 1 cut(s) 2838
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 3 cut(s) 232, 805, 3148
PspGI CCWGG 3 cut(s) 232, 805, 3148
PspN4I GGNNCC 6 cut(s) 167, 892, 1643, 1690, 2645, 3142
PspPI GGNCC 3 cut(s) 302, 1642, 3141
PspPPI RGGWCCY 1 cut(s) 302
PstI CTGCAG 1 cut(s) 1932
PstNI CAGNNNCTG 2 cut(s) 101, 704
PvuI CGATCG 1 cut(s) 369
PvuII CAGCTG 2 cut(s) 881, 3206
RruI TCGCGA 1 cut(s) 3032
RsaI GTAC 9 cut(s) 152, 247, 757, 840, 917, 1661, 1698, 2259, 3202
RsaNI GTAC 9 cut(s) 151, 246, 756, 839, 916, 1660, 1697, 2258, 3201
RseI CAYNNNNRTG 3 cut(s) 2356, 2859, 3314
SacI GAGCTC 3 cut(s) 339, 2709, 3282
SalI GTCGAC 1 cut(s) 568
SapI GCTCTTC 2 cut(s) 3037, 3287
SaqAI TTAA 9 cut(s) 137, 329, 410, 1290, 1422, 1707, 2271, 2897, 3258
SatI GCNGC 2 cut(s) 2102, 2105
Sau3AI GATC 9 cut(s) 21, 273, 366, 487, 1279, 1939, 2296, 2348, 3208
Sau96I GGNCC 3 cut(s) 302, 1642, 3141
SchI GAGTC 2 cut(s) 2075, 2886
ScrFI CCNGG 7 cut(s) 234, 462, 463, 721, 807, 1263, 3150
SduI GDGCHC 7 cut(s) 339, 1793, 2614, 2676, 2709, 2924, 3282
SfaNI GCATC 3 cut(s) 424, 1098, 1776
SfcI CTRYAG 2 cut(s) 90, 1928
Sfr274I CTCGAG 2 cut(s) 939, 1386
SfuI TTCGAA 1 cut(s) 818
SinI GGWCC 3 cut(s) 302, 1642, 3141
SlaI CTCGAG 2 cut(s) 939, 1386
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 3 cut(s) 2356, 2859, 3314
SmlI CTYRAG 7 cut(s) 391, 476, 623, 939, 1109, 1386, 1902
SmoI CTYRAG 7 cut(s) 391, 476, 623, 939, 1109, 1386, 1902
SnaBI TACGTA 1 cut(s) 1081
SphI GCATGC 1 cut(s) 1070
SsiI CCGC 5 cut(s) 324, 1577, 2099, 2107, 2691
SspMI CTAG 9 cut(s) 525, 1058, 1133, 1470, 2115, 2168, 2648, 2885, 3245
SstI GAGCTC 3 cut(s) 339, 2709, 3282
StyD4I CCNGG 7 cut(s) 232, 460, 461, 719, 805, 1261, 3148
StyI CCWWGG 4 cut(s) 2167, 2208, 2357, 3021
TaaI ACNGT 8 cut(s) 119, 548, 755, 1164, 1664, 1696, 2482, 2926
TaiI ACGT 6 cut(s) 1083, 1428, 2746, 2826, 2841, 3091
TatI WGTACW 6 cut(s) 245, 755, 838, 915, 2257, 3200
Tru1I TTAA 9 cut(s) 137, 329, 410, 1290, 1422, 1707, 2271, 2897, 3258
Tru9I TTAA 9 cut(s) 137, 329, 410, 1290, 1422, 1707, 2271, 2897, 3258
TscAI CASTG 7 cut(s) 91, 102, 124, 661, 1234, 1738, 2931
TseFI GTSAC 4 cut(s) 10, 45, 401, 2869
TseI GCWGC 2 cut(s) 2101, 2104
Tsp45I GTSAC 4 cut(s) 10, 45, 401, 2869
TspDTI ATGAA 5 cut(s) 70, 1308, 1574, 2020, 3248
TspGWI ACGGA 8 cut(s) 44, 178, 206, 347, 1578, 2888, 3024, 3171
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 7 cut(s) 91, 102, 124, 661, 1234, 1738, 2931
VneI GTGCAC 1 cut(s) 2672
VpaK11BI GGWCC 3 cut(s) 302, 1642, 3141
XapI RAATTY 4 cut(s) 800, 1004, 1176, 1710
XceI RCATGY 3 cut(s) 1070, 1261, 2238
XhoI CTCGAG 2 cut(s) 939, 1386
XmaI CCCGGG 1 cut(s) 461
XmaJI CCTAGG 1 cut(s) 2167
XmiI GTMKAC 2 cut(s) 569, 1602
XmnI GAANNNNTTC 1 cut(s) 2561
XspI CTAG 9 cut(s) 525, 1058, 1133, 1470, 2115, 2168, 2648, 2885, 3245
ZraI GACGTC 1 cut(s) 2824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.