RLG00000005136
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004694
Physical Location & Seq
Reverse (-)
65301601 .. 65305123
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005136

Sequence Viewer

Length: 3294 bp
ATGTACTCGAACTCGAAAACTCGTTCATGGCGCTCGGTGTTGTGCTCTTCCATGTTCTCACTCATCATCATGGTAACAGCTGAAGTTGTAGCTGGAGATTCTCTAACCACAGACATGGAAGTTCTGCTGAGCCTTAAAGCTTTTCTTCAAGAACAAAACAGAGTAAACCAAGGCCAATACTCAGAATGGAACCAGAGCATTAGCCCCTGTCATTGGCCTAGAATCAAATGCAATGGTGACAGTAGAGTTACTGGAATCGATCTCTCCGAAAGCAAAATCTCCGGTGAGTTTTTCAGAAACTTCTCTGCCTTGACACTGCTTTCGGACCTGGACTTGTCCGATAACACTCTAGGTGGAGCTATCCCGGAGGACTTGAGTCAATGCAAGAGCCTAAAGTACCTGAATTTGTCACATAACATTCTAGAAGGTAAGCTGAACTTGCAGGGGTTGAGTCAATTGGAGGTTCTTGATTTGGCTGTGAATAGGTTTTATGGGGATATGAAGATGAGCTTCCCTGGAATTTGCAAGAACTTGGTGGTGGCAAATGTTTCATCAAACAACTTTACTGGTAGGATTGACCAGTACTTTGATGAGTGCTCGAATTTGAAGTACTTGGATTTGAGCACAAACAAATTTAGTGGTGATGTGTGGGTTGGATTCACAAGGCTCAGGGAGTTTTCGGCATCTGAGAATTACCTCAGTGGGGCTATTTTGCCTTCTATTTTCAATGGCAGCTGTAACTTGGTGATTTTGGACCTGTCACAGAACAATTTCTCTGGTGGAGTTCCAAAGGAGATTTCAAATTGCCGGAATTTGGTTTCGTTGAATGTGTGGGGGAACAAATTTACAGGGCAAATTCCATCTGAGATTGGAAGCATTTCGAGTCTGGAGTCTTTGTTCTTTGGCAATAACAGTTTTTCTAGAGTGATCCCGGAAGCCCTTTTGAATTTGAGCAACTTGGAGTTCTTGGATTTTAGCAGGAACAATTTTAGAGGAGATATACAAGAGATTTTTGGGAGATTTACGCAGGTGAAGATTCTTGTGCTGCACTCAAATTCATACACAGGTGGTATAGCTGAAATTCTCAAGCTACCTAATGTTTATCGGTTAGATCTCAGTAACAACAATTTTACAGGTCCTTTACCGGTTGAAATTGCTAAAATGCCAAGATTGAAGTGCTTGTTCCTTGCCTACAATCAATTCAGTGGAACTATACCACCAGAGTATGGAAATTGGCAACAGCTCCAAGCACTGGACCTTTCTTTCAACAGACTAACCGGGTCAATCCCCGCCACCTTAGGAAAGTTGAGATCTCTCTTGTGGTTGATGCTTGCCAATAATTCTCTAACAGGTCCGATTCCTAGGGAGTTGGGGAACTGTACTAGTTTGTTGTGGCTGAACCTTTCCAACAACAAGCTCAATGGATCAATCCCATCTGAGTTGAAGAATATTGGGACAAATGCAGGTCCAACATTTGAATCTAACAATGATATCAAGTATGAGATCGTAGCTGGATCCGGGGAGTGCTCAGCAATGAAGAGATGGATTCCAGCAGACTACCCTCCATTCAGTTTTGTGTACACAATCCTCACAAGGAAGAGTTGTCGAAGTATATGGGATCGGCTACTTAAAGGAAATGGCCTTTTTCCCATATGTGTTGCTGGTTCTGCTGTAAGGACACTGCAAATCTCAGGTTATGTACAACTGAGTGGAAACCAGCTTTCAGGTCAGGTCCCTCCAGAGATTGGCGAGATGCGCAATTTTAGTATGATGAATTTGGCTTTCAATCAATTAAGTGGAGAACTCCCTGCCGGTATTGGACAAATGCCACTCGTAGTGTTTAACCTCAGTAAGAACAGTTTCTCGGGCGAAATCCCAATGGAAATTGGGAACAGTACGTGTATGCAGAATCTTGATTTGTCCTACAATAACTTTTCTGGTACATTTCCTGTAAGCTTAAACAGCTTGACTGAGCTAAGCAAGTTCAATATTTCATACAATCCACTCATCTCTGGTGTTATTCCTTCAAGTGGCCAATTGGCAACTTTTGAAAAGGAGTCCTACCTTGGTGACCCCCTCCTTATTCTTCCGAGTTTTATCACCAACTCCACGAATAATTCAACAAACAAGACAACTGGAAACCAAAAAAAGCCTGCAAAGCTTGGTGCTAATTTGGTGTTTCTAGTGCTAGTACTGACTTTCTTGATATGTGGAGTCTTTTCACTTACGATGTGCTTATTTATGAAGAGCCCAGCAGATGAGCCACAAGGGTATCTGCTGCCACACAGCAAATACCGGCTTGACCTTGCATCAAGTTCAAACTCTTCATCACCTTGGCTATCGGATACTGTCAAGGTCATTCGACTGGACAAAACAGCTTTCACACATGCTGACATTTTGAAGGCCACAGGAAATTTTTCAGAAGGGCGGATCATAGGGAGGGGAGGGTTTGGAACAGTGTACCAAGGAGTGTTGCCTGATGGGAGAGAAGTAGCAGTGAAGAAGCTTCAAAGAGAAGGACTTGAAGGTGAAAGGGAATTCAGAGCTGAAATGGAAGTTCTTACTGGAAATGGCTTTGGTTGGCCTCACCCAAATCTTGTGCAACTTCATGGCTGGTGTCATGATGGTTCAGAGAAAATATTAGTCTATGAGTACATGGAAGGTGGGAGCTTGGAGGATATTATATGTGACAAAGTAAGACTAAGATGGCGCAGACGGATTGATGTAGCAGTTGATGTGGCTCGAGCCTTGGTGTTTCTACACCATGAGTGCTTCCCTGCTATCGTGCACCGTGATGTGAAGGCTAGCAATGTGCTGCTAGATAAGGATGGGAAGGCAAGAGTGACAGATTTTGGGCTGGCTAGAATTGTTGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCTGGAACTGTTGGTTATGTTGCACCAGAATATGGGCAGACATGGCAAGCCACAACGAAAGGCGATGTGTACAGTTATGGAGTCCTAGCAATGGAACTGGCAACTGGGAGAAGAGCAGTGGATGGAGGAGAGGAGTGTCTAGTGGAATGGGCCAGAAGGGTGATGGGAAATGGACGGCTAGGATTCAATCGTTCTGTGATGCCGGTTGTGCTTATGGGGTCTGGACTAGTTGATGGGGCAGAAGAGATGTGTGAGTTACTAAGGGTTGGCATAAAGTGCACAGCTGAGGCACCACAGTCTAGACCAAATATGAAGGAAGTTCTAGCTATGCTGATAAAGATAGCCAATATCCAAGGGGATTTTAGTTGTGGTTCTCCTCCTTCATTTTGA

Protein Analysis

1098

Amino Acids

120.73

Weight (kDa)

6.27

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 85 - 139 4.7e-06 Leucine rich repeat
LRR_14 PF23598 384 - 471 5.6e-07 Leucine-rich repeat region
LRR_8 PF13855 414 - 473 1.4e-08 Leucine rich repeat
Pkinase PF00069 807 - 1076 7.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 809 - 1078 1.3e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1018
Acc16I TGCGCA 1 cut(s) 1757
Acc36I ACCTGC 2 cut(s) 1018, 1454
AccB1I GGYRCC 1 cut(s) 3193
AccB7I CCANNNNNTGG 4 cut(s) 1226, 1252, 1745, 2936
AciI CCGC 2 cut(s) 1290, 2428
AclWI GGATC 6 cut(s) 922, 1432, 1509, 1522, 1626, 2438
AcoI YGGCCR 1 cut(s) 2032
AcuI CTGAAG 1 cut(s) 102
AfiI CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1226, 1252, 1745, 2030, 2936, 2995
AflIII ACRYGT 1 cut(s) 1898
AgeI ACCGGT 1 cut(s) 1144
AhlI ACTAGT 2 cut(s) 1382, 3130
AjnI CCWGG 2 cut(s) 327, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 781, 813
AleI CACNNNNGTG 1 cut(s) 2900
AloI GAACNNNNNNTCC 2 cut(s) 881, 913
Alw21I GWGCWC 7 cut(s) 47, 599, 626, 1529, 2790, 2897, 3185
Alw44I GTGCAC 2 cut(s) 2786, 3181
AlwI GGATC 6 cut(s) 922, 1432, 1509, 1522, 1626, 2438
Ama87I CYCGRG 2 cut(s) 1864, 2742
AoxI GGCC 7 cut(s) 172, 215, 1639, 2032, 2403, 2582, 3054
ApaLI GTGCAC 2 cut(s) 2786, 3181
ApeKI GCWGC 4 cut(s) 732, 1045, 2278, 2815
ArsI GACNNNNNNTTYG 4 cut(s) 304, 336, 569, 601
AsiGI ACCGGT 1 cut(s) 1144
Asp700I GAANNNNTTC 4 cut(s) 770, 877, 1859, 2416
AspA2I CCTAGG 1 cut(s) 1361
AspLEI GCGC 3 cut(s) 33, 1758, 2712
AspS9I GGNCC 8 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732, 3054
AsuC2I CCSGG 4 cut(s) 365, 932, 1279, 1519
AsuNHI GCTAGC 1 cut(s) 2804
AvaI CYCGRG 2 cut(s) 1864, 2742
AvaII GGWCC 7 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732
AvrII CCTAGG 1 cut(s) 1361
AxyI CCTNAGG 1 cut(s) 1297
BaeGI GKGCMC 2 cut(s) 2790, 3185
BaeI ACNNNNGTAYC 2 cut(s) 2255, 2288
BalI TGGCCA 1 cut(s) 2034
BamHI GGATCC 1 cut(s) 1514
BanI GGYRCC 1 cut(s) 3193
BanII GRGCYC 1 cut(s) 2252
Bbv12I GWGCWC 7 cut(s) 47, 599, 626, 1529, 2790, 2897, 3185
BbvCI CCTCAGC 1 cut(s) 3189
BbvI GCAGC 4 cut(s) 744, 1032, 2265, 2802
BceAI ACGGC 1 cut(s) 3095
BciT130I CCWGG 2 cut(s) 329, 516
BciVI GTATCC 1 cut(s) 2338
BcnI CCSGG 4 cut(s) 365, 932, 1279, 1519
BcuI ACTAGT 2 cut(s) 1382, 3130
BfoI RGCGCY 1 cut(s) 34
BfuAI ACCTGC 2 cut(s) 1018, 1454
BfuI GTATCC 1 cut(s) 2338
BglII AGATCT 2 cut(s) 1111, 1310
BisI GCNGC 4 cut(s) 733, 1046, 2279, 2816
BlnI CCTAGG 1 cut(s) 1361
BlpI GCTNAGC 3 cut(s) 128, 1528, 1976
BlsI GCNGC 4 cut(s) 734, 1047, 2280, 2817
BmcAI AGTACT 3 cut(s) 584, 611, 2193
Bme1390I CCNGG 6 cut(s) 329, 365, 516, 932, 1279, 1519
Bme18I GGWCC 7 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732
BmeT110I CYCGRG 2 cut(s) 1864, 2742
BmgT120I GGNCC 8 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732, 3054
BmiI GGNNCC 4 cut(s) 191, 1516, 1734, 3195
BmrFI CCNGG 6 cut(s) 329, 365, 516, 932, 1279, 1519
BmrI ACTGGG 1 cut(s) 3018
BmsI GCATC 6 cut(s) 692, 1317, 1743, 2318, 2863, 3093
BmtI GCTAGC 1 cut(s) 2808
BmuI ACTGGG 1 cut(s) 3018
BoxI GACNNNNGTC 1 cut(s) 375
BpmI CTGGAG 3 cut(s) 114, 908, 1722
Bpu10I CCTNAGC 2 cut(s) 668, 3189
Bpu1102I GCTNAGC 3 cut(s) 128, 1528, 1976
BpuEI CTTGAG 2 cut(s) 394, 1070
BpuMI CCSGG 4 cut(s) 365, 932, 1279, 1519
Bsa29I ATCGAT 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 1899
BsaJI CCNNGG 9 cut(s) 169, 514, 1361, 1518, 2065, 2333, 2464, 2748, 3256
BsaWI WCCGGW 2 cut(s) 281, 1144
BsaXI ACNNNNNCTCC 2 cut(s) 881, 911
Bsc4I CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1226, 1252, 1745, 2030, 2936, 2995
Bse118I RCCGGY 4 cut(s) 1144, 1811, 2295, 3106
Bse1I ACTGG 9 cut(s) 256, 571, 580, 1257, 2140, 2370, 2569, 3006, 3013
Bse21I CCTNAGG 1 cut(s) 1297
Bse3DI GCAATG 4 cut(s) 238, 1539, 2815, 3000
BseBI CCWGG 2 cut(s) 329, 516
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 9 cut(s) 169, 514, 1361, 1518, 2065, 2333, 2464, 2748, 3256
BseGI GGATG 2 cut(s) 2833, 3031
BseLI CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1226, 1252, 1745, 2030, 2936, 2995
BseMI GCAATG 4 cut(s) 238, 1539, 2815, 3000
BseNI ACTGG 9 cut(s) 256, 571, 580, 1257, 2140, 2370, 2569, 3006, 3013
BseRI GAGGAG 4 cut(s) 1008, 3045, 3050, 3270
BseSI GKGCMC 2 cut(s) 2790, 3185
BseXI GCAGC 4 cut(s) 744, 1032, 2265, 2802
BseYI CCCAGC 1 cut(s) 2251
BsgI GTGCAG 1 cut(s) 1031
BshFI GGCC 7 cut(s) 174, 217, 1641, 2034, 2405, 2584, 3056
BshNI GGYRCC 1 cut(s) 3193
BshTI ACCGGT 1 cut(s) 1144
BshVI ATCGAT 1 cut(s) 258
BsiHKAI GWGCWC 7 cut(s) 47, 599, 626, 1529, 2790, 2897, 3185
BsiHKCI CYCGRG 2 cut(s) 1864, 2742
BslFI GGGAC 2 cut(s) 1468, 1718
BslI CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1226, 1252, 1745, 2030, 2936, 2995
BsmFI GGGAC 2 cut(s) 1468, 1718
BsnI GGCC 7 cut(s) 174, 217, 1641, 2034, 2405, 2584, 3056
BsoBI CYCGRG 2 cut(s) 1864, 2742
Bsp1286I GDGCHC 8 cut(s) 47, 599, 626, 1529, 2252, 2790, 2897, 3185
Bsp1407I TGTACA 3 cut(s) 1578, 1699, 2973
Bsp143I GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
Bsp1720I GCTNAGC 3 cut(s) 128, 1528, 1976
BspACI CCGC 2 cut(s) 1290, 2428
BspANI GGCC 7 cut(s) 174, 217, 1641, 2034, 2405, 2584, 3056
BspDI ATCGAT 1 cut(s) 258
BspHI TCATGA 1 cut(s) 2620
BspLI GGNNCC 4 cut(s) 191, 1516, 1734, 3195
BspMI ACCTGC 2 cut(s) 1018, 1454
BspOI GCTAGC 1 cut(s) 2808
BspPI GGATC 6 cut(s) 922, 1432, 1509, 1522, 1626, 2438
BspQI GCTCTTC 3 cut(s) 52, 2240, 3010
BspT107I GGYRCC 1 cut(s) 3193
BsrDI GCAATG 4 cut(s) 238, 1539, 2815, 3000
BsrFI RCCGGY 4 cut(s) 1144, 1811, 2295, 3106
BsrGI TGTACA 3 cut(s) 1578, 1699, 2973
BsrI ACTGG 9 cut(s) 256, 571, 580, 1257, 2140, 2370, 2569, 3006, 3013
BssAI RCCGGY 4 cut(s) 1144, 1811, 2295, 3106
BssECI CCNNGG 9 cut(s) 169, 514, 1361, 1518, 2065, 2333, 2464, 2748, 3256
BssMI GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
BssT1I CCWWGG 7 cut(s) 169, 1361, 2065, 2333, 2464, 2748, 3256
Bst2UI CCWGG 2 cut(s) 329, 516
Bst6I CTCTTC 7 cut(s) 52, 1532, 1592, 2240, 2329, 3010, 3141
BstAPI GCANNNNNTGC 1 cut(s) 3180
BstAUI TGTACA 3 cut(s) 1578, 1699, 2973
BstBAI YACGTR 1 cut(s) 1899
BstC8I GCNNGC 5 cut(s) 1332, 2154, 2806, 2859, 2952
BstEII GGTNACC 1 cut(s) 2069
BstF5I GGATG 2 cut(s) 2833, 3031
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 3 cut(s) 33, 1758, 2712
BstKTI GATC 9 cut(s) 262, 930, 1114, 1313, 1427, 1506, 1517, 1621, 2433
BstMBI GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
BstMWI GCNNNNNNNGC 8 cut(s) 439, 1667, 1755, 1962, 2158, 2947, 3112, 3180
BstNI CCWGG 2 cut(s) 329, 516
BstNSI RCATGY 1 cut(s) 2390
BstPAI GACNNNNGTC 1 cut(s) 375
BstPI GGTNACC 1 cut(s) 2069
BstSCI CCNGG 6 cut(s) 327, 363, 514, 930, 1277, 1517
BstSLI GKGCMC 2 cut(s) 2790, 3185
BstV1I GCAGC 4 cut(s) 744, 1032, 2265, 2802
BstX2I RGATCY 3 cut(s) 1111, 1310, 1514
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 3 cut(s) 1111, 1310, 1514
Bsu15I ATCGAT 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 1297
BsuI GTATCC 1 cut(s) 2338
BsuRI GGCC 7 cut(s) 174, 217, 1641, 2034, 2405, 2584, 3056
BsuTUI ATCGAT 1 cut(s) 258
BtgZI GCGATG 1 cut(s) 2982
BtsCI GGATG 2 cut(s) 2833, 3031
BtsI GCAGTG 4 cut(s) 314, 1679, 2502, 3027
BtsIMutI CAGTG 8 cut(s) 314, 706, 1210, 1250, 1679, 2463, 2502, 3027
BveI ACCTGC 2 cut(s) 1018, 1454
Cac8I GCNNGC 5 cut(s) 1332, 2154, 2806, 2859, 2952
CciI TCATGA 1 cut(s) 2620
CfoI GCGC 3 cut(s) 33, 1758, 2712
Cfr10I RCCGGY 4 cut(s) 1144, 1811, 2295, 3106
Cfr13I GGNCC 8 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732, 3054
ClaI ATCGAT 1 cut(s) 258
CspAI ACCGGT 1 cut(s) 1144
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
DpnI GATC 9 cut(s) 261, 929, 1113, 1312, 1426, 1505, 1516, 1620, 2432
DpnII GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
EaeI YGGCCR 1 cut(s) 2032
Eam1104I CTCTTC 7 cut(s) 52, 1532, 1592, 2240, 2329, 3010, 3141
EarI CTCTTC 7 cut(s) 52, 1532, 1592, 2240, 2329, 3010, 3141
EciI GGCGGA 1 cut(s) 2443
Eco130I CCWWGG 7 cut(s) 169, 1361, 2065, 2333, 2464, 2748, 3256
Eco24I GRGCYC 1 cut(s) 2252
Eco32I GATATC 1 cut(s) 1492
Eco47I GGWCC 7 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732
Eco57I CTGAAG 1 cut(s) 102
Eco81I CCTNAGG 1 cut(s) 1297
Eco88I CYCGRG 2 cut(s) 1864, 2742
Eco91I GGTNACC 1 cut(s) 2069
EcoO109I RGGNCCY 2 cut(s) 1136, 1732
EcoO65I GGTNACC 1 cut(s) 2069
EcoRI GAATTC 1 cut(s) 2537
EcoRII CCWGG 2 cut(s) 327, 514
EcoRV GATATC 1 cut(s) 1492
EcoT14I CCWWGG 7 cut(s) 169, 1361, 2065, 2333, 2464, 2748, 3256
EcoT38I GRGCYC 1 cut(s) 2252
ErhI CCWWGG 7 cut(s) 169, 1361, 2065, 2333, 2464, 2748, 3256
FalI AAGNNNNNCTT 6 cut(s) 129, 161, 422, 454, 494, 526
FaqI GGGAC 2 cut(s) 1468, 1718
FauI CCCGC 1 cut(s) 1297
FauNDI CATATG 1 cut(s) 1652
Fnu4HI GCNGC 4 cut(s) 733, 1046, 2279, 2816
FokI GGATG 2 cut(s) 2840, 3038
FriOI GRGCYC 1 cut(s) 2252
Fsp4HI GCNGC 4 cut(s) 733, 1046, 2279, 2816
FspI TGCGCA 1 cut(s) 1757
GlaI GCGC 3 cut(s) 32, 1757, 2711
GluI GCNGC 4 cut(s) 733, 1046, 2279, 2816
GsaI CCCAGC 1 cut(s) 2255
GsuI CTGGAG 3 cut(s) 114, 908, 1722
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 7 cut(s) 174, 217, 1641, 2034, 2405, 2584, 3056
HhaI GCGC 3 cut(s) 33, 1758, 2712
Hin6I GCGC 3 cut(s) 31, 1756, 2710
HinP1I GCGC 3 cut(s) 31, 1756, 2710
HindIII AAGCTT 4 cut(s) 138, 1954, 2159, 2504
Hpy166II GTNNAC 7 cut(s) 166, 1579, 1581, 2461, 2788, 2974, 3183
Hpy8I GTNNAC 7 cut(s) 166, 1579, 1581, 2461, 2788, 2974, 3183
HpyCH4IV ACGT 1 cut(s) 1898
HpyF10VI GCNNNNNNNGC 8 cut(s) 439, 1667, 1755, 1962, 2158, 2947, 3112, 3180
HpySE526I ACGT 1 cut(s) 1898
HspAI GCGC 3 cut(s) 31, 1756, 2710
Kzo9I GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
LguI GCTCTTC 3 cut(s) 52, 2240, 3010
LmnI GCTCC 3 cut(s) 356, 1248, 2667
Lsp1109I GCAGC 4 cut(s) 744, 1032, 2265, 2802
LweI GCATC 6 cut(s) 692, 1317, 1743, 2318, 2863, 3093
MaeII ACGT 1 cut(s) 1898
MalI GATC 9 cut(s) 261, 929, 1113, 1312, 1426, 1505, 1516, 1620, 2432
MboI GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
MfeI CAATTG 2 cut(s) 455, 2036
MflI RGATCY 3 cut(s) 1111, 1310, 1514
MhlI GDGCHC 8 cut(s) 47, 599, 626, 1529, 2252, 2790, 2897, 3185
MlsI TGGCCA 1 cut(s) 2034
MluNI TGGCCA 1 cut(s) 2034
MlyI GAGTC 7 cut(s) 385, 460, 892, 899, 2066, 2223, 2994
MmeI TCCRAC 3 cut(s) 634, 1431, 1493
Mox20I TGGCCA 1 cut(s) 2034
MroXI GAANNNNTTC 4 cut(s) 770, 877, 1859, 2416
MscI TGGCCA 1 cut(s) 2034
MseI TTAA 5 cut(s) 135, 1629, 1793, 1842, 1958
MslI CAYNNNNRTG 4 cut(s) 68, 113, 2793, 2900
Msp20I TGGCCA 1 cut(s) 2034
MspA1I CMGCKG 3 cut(s) 80, 735, 3188
MspR9I CCNGG 6 cut(s) 329, 365, 516, 932, 1279, 1519
MunI CAATTG 2 cut(s) 455, 2036
MvaI CCWGG 2 cut(s) 329, 516
MwoI GCNNNNNNNGC 8 cut(s) 439, 1667, 1755, 1962, 2158, 2947, 3112, 3180
NciI CCSGG 4 cut(s) 365, 932, 1279, 1519
NdeI CATATG 1 cut(s) 1652
NdeII GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
NheI GCTAGC 1 cut(s) 2804
NlaIV GGNNCC 4 cut(s) 191, 1516, 1734, 3195
NmuCI GTSAC 6 cut(s) 236, 408, 759, 2069, 2687, 2842
NsbI TGCGCA 1 cut(s) 1757
NspI RCATGY 1 cut(s) 2390
OliI CACNNNNGTG 1 cut(s) 2900
PaeR7I CTCGAG 1 cut(s) 2742
PagI TCATGA 1 cut(s) 2620
PaqCI CACCTGC 1 cut(s) 1018
PciSI GCTCTTC 3 cut(s) 52, 2240, 3010
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PdmI GAANNNNTTC 4 cut(s) 770, 877, 1859, 2416
PflMI CCANNNNNTGG 4 cut(s) 1226, 1252, 1745, 2936
PfoI TCCNGGA 2 cut(s) 363, 930
PinAI ACCGGT 1 cut(s) 1144
PkrI GCNGC 4 cut(s) 734, 1047, 2280, 2817
PleI GAGTC 7 cut(s) 384, 459, 891, 898, 2065, 2222, 2993
PpsI GAGTC 7 cut(s) 384, 459, 891, 898, 2065, 2222, 2993
Ppu21I YACGTR 1 cut(s) 1899
PpuMI RGGWCCY 2 cut(s) 1136, 1732
PshAI GACNNNNGTC 1 cut(s) 375
Psp5II RGGWCCY 2 cut(s) 1136, 1732
Psp6I CCWGG 2 cut(s) 327, 514
PspEI GGTNACC 1 cut(s) 2069
PspFI CCCAGC 1 cut(s) 2251
PspGI CCWGG 2 cut(s) 327, 514
PspN4I GGNNCC 4 cut(s) 191, 1516, 1734, 3195
PspPI GGNCC 8 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732, 3054
PspPPI RGGWCCY 2 cut(s) 1136, 1732
PspXI VCTCGAGB 1 cut(s) 2742
PsuI RGATCY 3 cut(s) 1111, 1310, 1514
PvuII CAGCTG 3 cut(s) 80, 735, 3188
RseI CAYNNNNRTG 4 cut(s) 68, 113, 2793, 2900
SapI GCTCTTC 3 cut(s) 52, 2240, 3010
SaqAI TTAA 5 cut(s) 135, 1629, 1793, 1842, 1958
SatI GCNGC 4 cut(s) 733, 1046, 2279, 2816
Sau3AI GATC 9 cut(s) 259, 927, 1111, 1310, 1424, 1503, 1514, 1618, 2430
Sau96I GGNCC 8 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732, 3054
ScaI AGTACT 3 cut(s) 584, 611, 2193
SchI GAGTC 7 cut(s) 385, 460, 892, 899, 2066, 2223, 2994
ScrFI CCNGG 6 cut(s) 329, 365, 516, 932, 1279, 1519
SduI GDGCHC 8 cut(s) 47, 599, 626, 1529, 2252, 2790, 2897, 3185
SfaNI GCATC 6 cut(s) 692, 1317, 1743, 2318, 2863, 3093
Sfr274I CTCGAG 1 cut(s) 2742
SinI GGWCC 7 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732
SlaI CTCGAG 1 cut(s) 2742
SmiMI CAYNNNNRTG 4 cut(s) 68, 113, 2793, 2900
SmlI CTYRAG 3 cut(s) 373, 1085, 2742
SmoI CTYRAG 3 cut(s) 373, 1085, 2742
SpeI ACTAGT 2 cut(s) 1382, 3130
SsiI CCGC 2 cut(s) 1290, 2428
SspI AATATT 3 cut(s) 1450, 1990, 2640
StyD4I CCNGG 6 cut(s) 327, 363, 514, 930, 1277, 1517
StyI CCWWGG 7 cut(s) 169, 1361, 2065, 2333, 2464, 2748, 3256
TaiI ACGT 1 cut(s) 1901
TaqI TCGA 8 cut(s) 8, 14, 258, 599, 881, 1606, 2362, 2743
TatI WGTACW 9 cut(s) 3, 582, 609, 1379, 1578, 1699, 2191, 2652, 2973
Tru1I TTAA 5 cut(s) 135, 1629, 1793, 1842, 1958
Tru9I TTAA 5 cut(s) 135, 1629, 1793, 1842, 1958
TscAI CASTG 8 cut(s) 321, 706, 1210, 1257, 1686, 2463, 2502, 3027
TseFI GTSAC 6 cut(s) 236, 408, 759, 2069, 2687, 2842
TseI GCWGC 4 cut(s) 732, 1045, 2278, 2815
Tsp45I GTSAC 6 cut(s) 236, 408, 759, 2069, 2687, 2842
TspGWI ACGGA 1 cut(s) 2731
TspRI CASTG 8 cut(s) 321, 706, 1210, 1257, 1686, 2463, 2502, 3027
Van91I CCANNNNNTGG 4 cut(s) 1226, 1252, 1745, 2936
VneI GTGCAC 2 cut(s) 2786, 3181
VpaK11BI GGWCC 7 cut(s) 325, 754, 1136, 1255, 1352, 1466, 1732
XbaI TCTAGA 3 cut(s) 421, 920, 3203
XceI RCATGY 1 cut(s) 2390
XcmI CCANNNNNNNNNTGG 1 cut(s) 3064
XhoI CTCGAG 1 cut(s) 2742
XmaJI CCTAGG 1 cut(s) 1361
XmnI GAANNNNTTC 4 cut(s) 770, 877, 1859, 2416
ZrmI AGTACT 3 cut(s) 584, 611, 2193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.