RchiOBHm_Chr7g0203471

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013734
Physical Location & Seq
Forward (+)
21176286 .. 21177274
989 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18212

Sequence Viewer

Length: 921 bp
ATGTCAATCAGTCTCCAATTGCTAGGAAATAATCTTAATGGCAGCATCCCTTCGAGCCTGGGAAAATGCCATAGGTTGCTAGAATTGAATCTTGCTCAAAATAACCTTGACGGCACAATACCTCAACAACTACTCATTGGCCTTCCCTTATCAATTTATTTGTACTTTCAGGAAAATCGCTTTAGTGGATCCCTGCCTGTGGAGGTTGGAAAGTTGAAGTCTCTACGTGTAATAGACATTCTTGATAACATGTTATCTGGAGAATTTCCCAATAGCCTTGGTGGTTGTGAGAATTTAGAAGCCCTACACTTGCAAGGCAACTTCTTCAAGGGTCCAATTCCTTCATCTATGAGTGCATTGAGAGGGATTCAAGAGTTAGACCTTTCTTGCAACAATTTGTCTGGTGAAATTCCACAGTTTTTAGAGAGCTTTGTCTTCTTGAAGAATCTGAACTTATCTTTCAATCAGTTTTGGGGTGAAGTACATATTAAAAAGCTTCCAACATGCAAGTCTAAAGGAGGAGGATTATCTCATAGAATGAAAGTAATGATCTCTTTAGTATCTGTGTTTGCTGCTATAGGAATAGCTATGGTACTGTCAGTTTTATTTCTTTTTTCATCGAGAACGAAAAGGAAAGAAACCACTTTGAGCAATTTTGGGGACTCCATTTTGCAAGTTTCATTTACTGATCTTTTTAAAGCTACTGATGAGTTCTGTTCAACTAATTTGATTGGTATGCATGGGTGGTTTGAGTCTGTGTATAAAGGAGTTCTAGATGATGGTAGAGTTGTTGCTGTGAAGGTGTTTAACATGATACAACGGGGAGCTTCAAAGAGCTTCATAGCTGAACGTGAGGCACTGCGAAATATCAGGCATCGAAATCTAGTCAAAATTATAAGTGCATGTTCATCGATCTATTGA

Protein Analysis

306

Amino Acids

33.84

Weight (kDa)

9.01

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 22 - 166 7e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
AclWI GGATC 2 cut(s) 183, 196
AcsI RAATTY 3 cut(s) 263, 292, 408
AfaI GTAC 3 cut(s) 164, 483, 594
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 2 cut(s) 226, 249
AgsI TTSAA 8 cut(s) 88, 217, 328, 371, 442, 463, 720, 831
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 7 cut(s) 429, 496, 587, 701, 827, 837, 845
AluI AGCT 7 cut(s) 429, 496, 587, 701, 827, 837, 845
Alw26I GTCTC 2 cut(s) 17, 225
AlwI GGATC 2 cut(s) 183, 196
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 42, 572
ApoI RAATTY 3 cut(s) 263, 292, 408
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 416, 488
AvaII GGWCC 1 cut(s) 332
BamHI GGATCC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 427
BbvI GCAGC 2 cut(s) 54, 559
BccI CCATC 1 cut(s) 773
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 1 cut(s) 891
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 2 cut(s) 17, 225
BfaI CTAG 4 cut(s) 23, 80, 773, 884
BfmI CTRYAG 1 cut(s) 576
BisI GCNGC 2 cut(s) 43, 573
BlsI GCNGC 2 cut(s) 44, 574
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 190, 333
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 2 cut(s) 54, 883
BpiI GAAGAC 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 279
Bsa29I ATCGAT 1 cut(s) 911
BsaAI YACGTR 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 58, 277
Bsc4I CCNNNNNNNGG 1 cut(s) 199
BseBI CCWGG 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 911
BseDI CCNNGG 2 cut(s) 58, 277
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 534
BseXI GCAGC 2 cut(s) 54, 559
BshFI GGCC 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 911
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 17, 225
BsmFI GGGAC 1 cut(s) 674
BsnI GGCC 1 cut(s) 141
Bsp143I GATC 4 cut(s) 188, 549, 688, 912
BspANI GGCC 1 cut(s) 141
BspDI ATCGAT 1 cut(s) 911
BspLI GGNNCC 2 cut(s) 190, 333
BspPI GGATC 2 cut(s) 183, 196
BssECI CCNNGG 2 cut(s) 58, 277
BssMI GATC 4 cut(s) 188, 549, 688, 912
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 2 cut(s) 417, 597
BstBAI YACGTR 1 cut(s) 227
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 4 cut(s) 191, 552, 691, 915
BstMAI GTCTC 2 cut(s) 17, 225
BstMBI GATC 4 cut(s) 188, 549, 688, 912
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 3 cut(s) 253, 507, 906
BstSCI CCNGG 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 576
BstV1I GCAGC 2 cut(s) 54, 559
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 911
BsuRI GGCC 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 911
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 857
BtsIMutI CAGTG 1 cut(s) 857
Cfr13I GGNCC 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 911
Csp6I GTAC 3 cut(s) 163, 482, 593
CviAII CATG 5 cut(s) 250, 504, 740, 811, 903
CviQI GTAC 3 cut(s) 163, 482, 593
DpnI GATC 4 cut(s) 190, 551, 690, 914
DpnII GATC 4 cut(s) 188, 549, 688, 912
DraI TTTAAA 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 277
EcoT22I ATGCAT 1 cut(s) 741
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 5 cut(s) 253, 507, 743, 814, 906
FaqI GGGAC 1 cut(s) 674
FatI CATG 5 cut(s) 249, 503, 739, 810, 902
Fnu4HI GCNGC 2 cut(s) 43, 573
FokI GGATG 1 cut(s) 32
Fsp4HI GCNGC 2 cut(s) 43, 573
FspBI CTAG 4 cut(s) 23, 80, 773, 884
GluI GCNGC 2 cut(s) 43, 573
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 1 cut(s) 141
Hin1II CATG 5 cut(s) 253, 507, 743, 814, 906
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 5 cut(s) 88, 367, 445, 662, 752
HphI GGTGA 2 cut(s) 416, 488
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 7 cut(s) 170, 242, 258, 371, 439, 621, 773
HpyAV CCTTC 4 cut(s) 60, 152, 351, 793
HpyCH4III ACNGT 2 cut(s) 417, 597
HpyCH4IV ACGT 2 cut(s) 226, 850
HpyCH4V TGCA 7 cut(s) 313, 356, 390, 507, 673, 739, 902
HpySE526I ACGT 2 cut(s) 226, 850
Hsp92II CATG 5 cut(s) 253, 507, 743, 814, 906
Kzo9I GATC 4 cut(s) 188, 549, 688, 912
LmnI GCTCC 1 cut(s) 824
LpnPI CCDG 8 cut(s) 44, 71, 155, 206, 210, 243, 387, 856
Lsp1109I GCAGC 2 cut(s) 54, 559
LweI GCATC 2 cut(s) 54, 883
MaeI CTAG 4 cut(s) 23, 80, 773, 884
MaeII ACGT 2 cut(s) 226, 850
MalI GATC 4 cut(s) 190, 551, 690, 914
MboI GATC 4 cut(s) 188, 549, 688, 912
MboII GAAGA 3 cut(s) 316, 427, 454
MfeI CAATTG 1 cut(s) 17
MflI RGATCY 1 cut(s) 188
MlyI GAGTC 2 cut(s) 656, 761
MmeI TCCRAC 2 cut(s) 187, 524
MnlI CCTC 6 cut(s) 132, 196, 356, 512, 515, 847
Mph1103I ATGCAT 1 cut(s) 741
MseI TTAA 4 cut(s) 36, 489, 696, 807
MspR9I CCNGG 1 cut(s) 59
MunI CAATTG 1 cut(s) 17
MvaI CCWGG 1 cut(s) 59
NdeII GATC 4 cut(s) 188, 549, 688, 912
NlaIII CATG 5 cut(s) 253, 507, 743, 814, 906
NlaIV GGNNCC 2 cut(s) 190, 333
NsiI ATGCAT 1 cut(s) 741
NspI RCATGY 3 cut(s) 253, 507, 906
PciI ACATGT 1 cut(s) 249
PfeI GAWTC 3 cut(s) 88, 367, 445
PkrI GCNGC 2 cut(s) 44, 574
PleI GAGTC 2 cut(s) 656, 760
PpsI GAGTC 2 cut(s) 656, 760
Ppu21I YACGTR 1 cut(s) 227
PscI ACATGT 1 cut(s) 249
PsiI TTATAA 1 cut(s) 896
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 190, 333
PspPI GGNCC 1 cut(s) 332
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 3 cut(s) 164, 483, 594
RsaNI GTAC 3 cut(s) 163, 482, 593
SaqAI TTAA 4 cut(s) 36, 489, 696, 807
SatI GCNGC 2 cut(s) 43, 573
Sau3AI GATC 4 cut(s) 188, 549, 688, 912
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 2 cut(s) 656, 761
ScrFI CCNGG 1 cut(s) 59
SfaNI GCATC 2 cut(s) 54, 883
SfcI CTRYAG 1 cut(s) 576
SinI GGWCC 1 cut(s) 332
SspMI CTAG 4 cut(s) 23, 80, 773, 884
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 2 cut(s) 417, 597
TaiI ACGT 2 cut(s) 229, 853
TaqI TCGA 4 cut(s) 53, 620, 877, 911
TatI WGTACW 2 cut(s) 162, 481
TfiI GAWTC 3 cut(s) 88, 367, 445
Tru1I TTAA 4 cut(s) 36, 489, 696, 807
Tru9I TTAA 4 cut(s) 36, 489, 696, 807
TscAI CASTG 1 cut(s) 864
TseI GCWGC 2 cut(s) 42, 572
TspDTI ATGAA 6 cut(s) 333, 554, 606, 669, 829, 897
TspRI CASTG 1 cut(s) 864
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 3 cut(s) 263, 292, 408
XbaI TCTAGA 1 cut(s) 772
XceI RCATGY 3 cut(s) 253, 507, 906
XspI CTAG 4 cut(s) 23, 80, 773, 884
Zsp2I ATGCAT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.