Rorug07G0076100

LRR receptor-like serine threonine-protein kinase At3g47570

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013734
Physical Location & Seq
Forward (+)
5845780 .. 5850119
4340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0076100.1

Sequence Viewer

Length: 2208 bp
ATGGAGACCCAATCTGGCGGCAAAGAAGACACGGCCATCAAAGCAATGCGCCAAGACCTCGAAACCCGAATGGAGTCCCAACGCGCCACCCAGCTCGAACTCCTCTCTTATCTCCAATCCGAAATCGACCCCGCCATCGTCCCCGCCATCGATCTCTCCCTCAAGGTCCTCTCCTCCTTCAACAACCGCCCCTTCACTCCCACCCCTCCTCTACCCGACCCCAAGCCCAACCCCAGAAAGCCCATCGAGCCCGCCCGCCCTCCCTCCCCACACCCACACCCACAACAGCCCCTCCTCCCTTCGCCCGCCTCCCCCGAAAGCCCCGACCCGACCCGGGAATCCACCCCCGAGTTGGACGAGAGCGGGAACCCGCTTTCGGTGATCCGGGCCATGATTGCGGTTCGGTTTCTTGAGAGAGTGCCGTTTATGCTGGTGGAGTCGTCGGAGCTGCTGAAGCAGCTCGAGACCGACGCGAAGGCCTCGCCGGACGAGAAGCTGGCGCTGCGGGAGGTGGGAGGAGAGAACGGGGGGATTCTGGCGGTGGAGATGGCGCTGAGGTCGATGGCGGAGGAGAACGGCGGCGTTATCCTGGAGGAGTACGCGGTCAACGGAAAGTCAAGGGTTACGGTGAGGGATATTGACCGGACTAGGCTGATGAAGGAGTTGCCGGAGAGCAAGCAAGATTCGATTGTGGATGGGAATGGCAATGGGAATTTCAATCAGACTAGTGGCATGGATGTCGGGAATAACAATGGAGGAGGAGGATTTGTGGGGAGAGGTGGGCCGTGGGTGGTTGGGCCGGAGCCCTACATGAGCGGGTTGCCGCCATTGTTTCCGAGAGGGAGAGGGATGATAGGAATGCCGAGAGGGTCTCCCATGCATAGGCCTAATATGGGGCAGAATGGTGGTGGAATGGTGGTCAGTCCCAATGGAATGCCGAATAAGAGCATGAAGAGTGAAGAGGAAGAGATGAAGGATCTTGAGGCATTGCTGAGTAAGAAGACTTATAGGGAGTTGCAGAAGTCGAAAACTGGGGAGGAGCTTTTGGACCTCATTCACAGGCCAACTGCAAAGGAGACTGCTGTGGCAAACAAGTTCAAAACTAAAGGTGGTTCACAATTGAAGGAATACTGCACCTCCCTAACGAAAGAGGACTGTCGACGTCAATCTAATTCCTTGCTCGCTTGTGAGAAGGTTCATTTTAGGCGCATAATTGCTCTACATACTGATGTCAACTTGGGAGACTGTTCTTTTCTAGATACTTGCCGCCACATGAAGACATGCAAGTATGTACATTATGAGCTTGATTCAACACCAGATGTGTCAAACATGTTGGCTCCCCCTAAACCACTAAAACAGCGTGCTGAATATTGTTCTGAGGTAGAACTTGGTCAACCACAATGGATTAACTGTGATATCCGCAATTTTAGAATGGATATATTGGGACAGTTTGGAGTCATAATGGCTGATCCGCCATGGGACATTCATATGGAATTGCCTTATGGGACTATGGCTGATGATGAAATGCGCACTCTTAATGTTCCTGCATTGCAGACTGATGGTCTGATTTTCCTTTGGGTCACTGGACGTGCGATGGAGCTTGGACGTGAATGTTTAGAACTTTGGGGATACAAGCGTATTGAGGAGATGATTTGGGTGAAAACTAATCAACTGCAACGAATAATTAGAACTGGGCGCACTGGCCACTGGCTAAATCATAGCAAGGAGCATTGCCTCGTTGGAATAAAAGGAGATCCATTAGTAAATAGGAATATTGATACTGATGTCATTGTTGCTGAGGTCAGAGAGACAAGTCGTAAGCCAGACGAGATGTACCCTTTGCTGGAGAGGATTAGTCCAAGGACAAGAAAGCTGGAACTGTTTGCACGCATGCACAACACTCATGCAGGGTGGATGTCACTCGGTAACCAACTGAGTGGTGTGCGATTGGTTGATGAAGGCTTGCGGGCACGGTTCAAGGCTGCCTACCCAGAGGTGGAGGTGCAGCCAGCTTCCCCACCCAGAGCTTCTGCCATGGAAGTGGATTCAAATGCTGCCCAAACGAGAAGTCCATTTTCCGAAGCAAAGCCCACAGCTGCACAACTAGCAGAGGTTGCTGCTCCACAACAAGCAGAGCCTGCAGGGCCAGATGCGCCTGCTTCTGAGGGGAAGCCATCGTCTACCACCGATGTTGACATGGCCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

735

Amino Acids

81.36

Weight (kDa)

5.72

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MT-A70 PF05063 484 - 644 3.2e-60 MT-A70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1165
Acc16I TGCGCA 1 cut(s) 1529
AccBSI CCGCTC 2 cut(s) 363, 816
AccI GTMKAC 2 cut(s) 1159, 2180
AccII CGCG 3 cut(s) 84, 473, 602
AclWI GGATC 4 cut(s) 376, 984, 1463, 1748
AcoI YGGCCR 3 cut(s) 33, 1702, 2199
AcsI RAATTY 1 cut(s) 712
AcuI CTGAAG 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 1162
AfaI GTAC 3 cut(s) 599, 1293, 1835
AfiI CCNNNNNNNGG 7 cut(s) 334, 352, 376, 882, 893, 1843, 1996
AflIII ACRYGT 1 cut(s) 1329
AgsI TTSAA 7 cut(s) 181, 718, 1099, 1123, 1311, 1978, 2049
AhdI GACNNNNNGTC 1 cut(s) 1560
AhlI ACTAGT 1 cut(s) 725
AjiI CACGTC 2 cut(s) 1589, 1607
AjnI CCWGG 1 cut(s) 588
Alw26I GTCTC 5 cut(s) 458, 876, 1070, 1236, 1802
AlwI GGATC 4 cut(s) 376, 984, 1463, 1748
AlwNI CAGNNNCTG 1 cut(s) 2138
Ama87I CYCGRG 3 cut(s) 333, 347, 461
ApeKI GCWGC 8 cut(s) 448, 457, 502, 1982, 2005, 2054, 2096, 2117
ApoI RAATTY 1 cut(s) 712
ArsI GACNNNNNNTTYG 2 cut(s) 2053, 2085
AspLEI GCGC 8 cut(s) 51, 86, 502, 553, 1209, 1530, 1698, 2155
AspS9I GGNCC 6 cut(s) 166, 387, 782, 797, 1048, 2144
AsuC2I CCSGG 3 cut(s) 334, 335, 386
AsuHPI GGTGA 3 cut(s) 391, 640, 1669
AvaI CYCGRG 3 cut(s) 333, 347, 461
AvaII GGWCC 2 cut(s) 166, 1048
BaeGI GKGCMC 1 cut(s) 1972
BaeI ACNNNNGTAYC 4 cut(s) 1621, 1654, 1770, 1803
BalI TGGCCA 1 cut(s) 1704
BanII GRGCYC 2 cut(s) 252, 807
BbsI GAAGAC 3 cut(s) 33, 1007, 1283
BbvCI CCTCAGC 2 cut(s) 554, 1797
BbvI GCAGC 8 cut(s) 435, 469, 489, 1969, 2017, 2041, 2083, 2104
BceAI ACGGC 4 cut(s) 48, 406, 592, 769
BcgI CGANNNNNNTGC 2 cut(s) 721, 755
BciT130I CCWGG 1 cut(s) 590
BciVI GTATCC 1 cut(s) 1622
BcnI CCSGG 3 cut(s) 334, 335, 386
BcoDI GTCTC 5 cut(s) 458, 876, 1070, 1236, 1802
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 4 cut(s) 648, 726, 1256, 2105
BfmI CTRYAG 1 cut(s) 2139
BfoI RGCGCY 2 cut(s) 503, 554
BfuI GTATCC 1 cut(s) 1622
BglI GCCNNNNNGGC 1 cut(s) 2143
Bme1390I CCNGG 4 cut(s) 334, 335, 386, 590
Bme18I GGWCC 2 cut(s) 166, 1048
BmeRI GACNNNNNGTC 1 cut(s) 1560
BmeT110I CYCGRG 3 cut(s) 333, 347, 461
BmgBI CACGTC 2 cut(s) 1589, 1607
BmgT120I GGNCC 6 cut(s) 166, 387, 782, 797, 1048, 2144
BmiI GGNNCC 3 cut(s) 368, 804, 1338
BmrFI CCNGG 4 cut(s) 334, 335, 386, 590
BmrI ACTGGG 2 cut(s) 1041, 1701
BmsI GCATC 1 cut(s) 2140
BmuI ACTGGG 2 cut(s) 1041, 1701
BpiI GAAGAC 3 cut(s) 33, 1007, 1283
BplI GAGNNNNNCTC 2 cut(s) 856, 888
BpmI CTGGAG 2 cut(s) 611, 1865
Bpu10I CCTNAGC 2 cut(s) 554, 1797
BpuEI CTTGAG 3 cut(s) 146, 431, 1001
BpuMI CCSGG 3 cut(s) 334, 335, 386
Bsa29I ATCGAT 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 1162
BsaI GGTCTC 2 cut(s) 458, 876
BsaJI CCNNGG 5 cut(s) 333, 785, 1475, 1859, 2034
BsaWI WCCGGW 1 cut(s) 642
BsaXI ACNNNNNCTCC 6 cut(s) 276, 306, 753, 783, 1121, 1151
Bsc4I CCNNNNNNNGG 7 cut(s) 334, 352, 376, 882, 893, 1843, 1996
Bse118I RCCGGY 1 cut(s) 2201
Bse1I ACTGG 5 cut(s) 1036, 1588, 1696, 1705, 1712
Bse3DI GCAATG 5 cut(s) 51, 712, 986, 1547, 1729
BseBI CCWGG 1 cut(s) 590
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 5 cut(s) 333, 785, 1475, 1859, 2034
BseGI GGATG 4 cut(s) 700, 742, 855, 1920
BseLI CCNNNNNNNGG 7 cut(s) 334, 352, 376, 882, 893, 1843, 1996
BseMI GCAATG 5 cut(s) 51, 712, 986, 1547, 1729
BseMII CTCAG 6 cut(s) 545, 983, 1368, 1788, 1925, 2154
BseNI ACTGG 5 cut(s) 1036, 1588, 1696, 1705, 1712
BseSI GKGCMC 1 cut(s) 1972
BseXI GCAGC 8 cut(s) 435, 469, 489, 1969, 2017, 2041, 2083, 2104
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 3 cut(s) 1117, 2024, 2082
Bsh1236I CGCG 3 cut(s) 84, 473, 602
BshVI ATCGAT 1 cut(s) 150
BsiHKCI CYCGRG 3 cut(s) 333, 347, 461
BsiSI CCGG 7 cut(s) 334, 385, 485, 643, 668, 800, 2202
BslFI GGGAC 6 cut(s) 61, 125, 909, 1458, 1493, 1519
BslI CCNNNNNNNGG 7 cut(s) 334, 352, 376, 882, 893, 1843, 1996
BsmAI GTCTC 5 cut(s) 458, 876, 1070, 1236, 1802
BsmFI GGGAC 6 cut(s) 61, 125, 909, 1458, 1493, 1519
BsmI GAATGC 2 cut(s) 864, 939
Bso31I GGTCTC 2 cut(s) 458, 876
BsoBI CYCGRG 3 cut(s) 333, 347, 461
Bsp1286I GDGCHC 3 cut(s) 252, 807, 1972
Bsp1407I TGTACA 1 cut(s) 1291
Bsp143I GATC 5 cut(s) 151, 381, 976, 1468, 1753
Bsp19I CCATGG 2 cut(s) 1475, 2034
BspCNI CTCAG 6 cut(s) 546, 984, 1369, 1789, 1926, 2155
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 3 cut(s) 84, 473, 602
BspLI GGNNCC 3 cut(s) 368, 804, 1338
BspMAI CTGCAG 1 cut(s) 2143
BspPI GGATC 4 cut(s) 376, 984, 1463, 1748
BspTNI GGTCTC 2 cut(s) 458, 876
BsrBI CCGCTC 2 cut(s) 363, 816
BsrDI GCAATG 5 cut(s) 51, 712, 986, 1547, 1729
BsrFI RCCGGY 1 cut(s) 2201
BsrGI TGTACA 1 cut(s) 1291
BsrI ACTGG 5 cut(s) 1036, 1588, 1696, 1705, 1712
BssAI RCCGGY 1 cut(s) 2201
BssECI CCNNGG 5 cut(s) 333, 785, 1475, 1859, 2034
BssMI GATC 5 cut(s) 151, 381, 976, 1468, 1753
BssNI GRCGYC 1 cut(s) 1162
BssT1I CCWWGG 3 cut(s) 1475, 1859, 2034
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 7 cut(s) 628, 1157, 1247, 1412, 1449, 1881, 1974
Bst6I CTCTTC 3 cut(s) 947, 954, 960
BstACI GRCGYC 1 cut(s) 1162
BstAPI GCANNNNNTGC 2 cut(s) 2114, 2138
BstAUI TGTACA 1 cut(s) 1291
BstDEI CTNAG 6 cut(s) 554, 992, 1377, 1797, 1934, 2163
BstDSI CCRYGG 3 cut(s) 785, 1475, 2034
BstEII GGTNACC 1 cut(s) 1925
BstF5I GGATG 4 cut(s) 700, 742, 855, 1920
BstFNI CGCG 3 cut(s) 84, 473, 602
BstH2I RGCGCY 2 cut(s) 503, 554
BstHHI GCGC 8 cut(s) 51, 86, 502, 553, 1209, 1530, 1698, 2155
BstKTI GATC 5 cut(s) 154, 384, 979, 1471, 1756
BstMAI GTCTC 5 cut(s) 458, 876, 1070, 1236, 1802
BstMBI GATC 5 cut(s) 151, 381, 976, 1468, 1753
BstNI CCWGG 1 cut(s) 590
BstNSI RCATGY 3 cut(s) 1284, 1333, 1894
BstPI GGTNACC 1 cut(s) 1925
BstSCI CCNGG 4 cut(s) 332, 333, 384, 588
BstSFI CTRYAG 1 cut(s) 2139
BstSLI GKGCMC 1 cut(s) 1972
BstUI CGCG 3 cut(s) 84, 473, 602
BstV1I GCAGC 8 cut(s) 435, 469, 489, 1969, 2017, 2041, 2083, 2104
BstV2I GAAGAC 3 cut(s) 33, 1007, 1283
BstX2I RGATCY 2 cut(s) 976, 1753
BstXI CCANNNNNNTGG 2 cut(s) 1937, 2041
BstYI RGATCY 2 cut(s) 976, 1753
Bsu15I ATCGAT 1 cut(s) 150
BsuI GTATCC 1 cut(s) 1622
BsuTUI ATCGAT 1 cut(s) 150
BtgI CCRYGG 3 cut(s) 785, 1475, 2034
BtgZI GCGATG 1 cut(s) 1607
BtrI CACGTC 2 cut(s) 1589, 1607
BtsCI GGATG 4 cut(s) 700, 742, 855, 1920
BtsIMutI CAGTG 3 cut(s) 1581, 1698, 1705
CaiI CAGNNNCTG 1 cut(s) 2138
CfoI GCGC 8 cut(s) 51, 86, 502, 553, 1209, 1530, 1698, 2155
Cfr10I RCCGGY 1 cut(s) 2201
Cfr13I GGNCC 6 cut(s) 166, 387, 782, 797, 1048, 2144
Cfr9I CCCGGG 1 cut(s) 333
ClaI ATCGAT 1 cut(s) 150
CseI GACGC 1 cut(s) 479
Csp6I GTAC 3 cut(s) 598, 1292, 1834
CspCI CAANNNNNGTGG 2 cut(s) 2173, 2208
CviQI GTAC 3 cut(s) 598, 1292, 1834
DdeI CTNAG 6 cut(s) 554, 992, 1377, 1797, 1934, 2163
DpnI GATC 5 cut(s) 153, 383, 978, 1470, 1755
DpnII GATC 5 cut(s) 151, 381, 976, 1468, 1753
DriI GACNNNNNGTC 1 cut(s) 1560
EaeI YGGCCR 3 cut(s) 33, 1702, 2199
Eam1104I CTCTTC 3 cut(s) 947, 954, 960
Eam1105I GACNNNNNGTC 1 cut(s) 1560
EarI CTCTTC 3 cut(s) 947, 954, 960
EciI GGCGGA 2 cut(s) 581, 1461
Eco130I CCWWGG 3 cut(s) 1475, 1859, 2034
Eco147I AGGCCT 2 cut(s) 479, 886
Eco24I GRGCYC 2 cut(s) 252, 807
Eco31I GGTCTC 2 cut(s) 458, 876
Eco32I GATATC 1 cut(s) 1417
Eco47I GGWCC 2 cut(s) 166, 1048
Eco57I CTGAAG 1 cut(s) 473
Eco88I CYCGRG 3 cut(s) 333, 347, 461
Eco91I GGTNACC 1 cut(s) 1925
EcoO109I RGGNCCY 1 cut(s) 166
EcoO65I GGTNACC 1 cut(s) 1925
EcoRII CCWGG 1 cut(s) 588
EcoRV GATATC 1 cut(s) 1417
EcoT14I CCWWGG 3 cut(s) 1475, 1859, 2034
EcoT22I ATGCAT 1 cut(s) 882
EcoT38I GRGCYC 2 cut(s) 252, 807
ErhI CCWWGG 3 cut(s) 1475, 1859, 2034
FaqI GGGAC 6 cut(s) 61, 125, 909, 1458, 1493, 1519
FauNDI CATATG 1 cut(s) 1488
FblI GTMKAC 2 cut(s) 1159, 2180
FokI GGATG 4 cut(s) 707, 749, 862, 1927
FriOI GRGCYC 2 cut(s) 252, 807
FspAI RTGCGCAY 1 cut(s) 1529
FspBI CTAG 4 cut(s) 648, 726, 1256, 2105
FspI TGCGCA 1 cut(s) 1529
GlaI GCGC 8 cut(s) 50, 85, 501, 552, 1208, 1529, 1697, 2154
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 2 cut(s) 611, 1865
HaeII RGCGCY 2 cut(s) 503, 554
HapII CCGG 7 cut(s) 334, 385, 485, 643, 668, 800, 2202
HgaI GACGC 1 cut(s) 479
HhaI GCGC 8 cut(s) 51, 86, 502, 553, 1209, 1530, 1698, 2155
Hin1I GRCGYC 1 cut(s) 1162
Hin6I GCGC 8 cut(s) 49, 84, 500, 551, 1207, 1528, 1696, 2153
HinP1I GCGC 8 cut(s) 49, 84, 500, 551, 1207, 1528, 1696, 2153
HincII GTYRAC 5 cut(s) 607, 1160, 1234, 1394, 2194
HindII GTYRAC 5 cut(s) 607, 1160, 1234, 1394, 2194
HinfI GANTC 8 cut(s) 74, 338, 437, 532, 683, 1307, 1455, 2045
HpaII CCGG 7 cut(s) 334, 385, 485, 643, 668, 800, 2202
HphI GGTGA 3 cut(s) 391, 640, 1669
Hpy166II GTNNAC 7 cut(s) 607, 1115, 1160, 1234, 1394, 2181, 2194
Hpy188I TCNGA 9 cut(s) 121, 445, 723, 837, 1378, 1566, 1805, 2080, 2164
Hpy188III TCNNGA 5 cut(s) 410, 463, 742, 980, 1256
Hpy8I GTNNAC 7 cut(s) 607, 1115, 1160, 1234, 1394, 2181, 2194
Hpy99I CGWCG 3 cut(s) 445, 473, 1164
HpyAV CCTTC 9 cut(s) 187, 202, 309, 469, 652, 967, 1117, 1186, 1952
HpyCH4III ACNGT 7 cut(s) 628, 1157, 1247, 1412, 1449, 1881, 1974
HpyCH4IV ACGT 3 cut(s) 1162, 1588, 1606
HpyF3I CTNAG 6 cut(s) 554, 992, 1377, 1797, 1934, 2163
HpySE526I ACGT 3 cut(s) 1162, 1588, 1606
Hsp92I GRCGYC 1 cut(s) 1162
HspAI GCGC 8 cut(s) 49, 84, 500, 551, 1207, 1528, 1696, 2153
KroI GCCGGC 1 cut(s) 2201
KroNI GCCGGC 1 cut(s) 2203
Kzo9I GATC 5 cut(s) 151, 381, 976, 1468, 1753
LmnI GCTCC 7 cut(s) 445, 802, 1039, 1342, 1597, 1726, 2125
Lsp1109I GCAGC 8 cut(s) 435, 469, 489, 1969, 2017, 2041, 2083, 2104
LweI GCATC 1 cut(s) 2140
MaeI CTAG 4 cut(s) 648, 726, 1256, 2105
MaeII ACGT 3 cut(s) 1162, 1588, 1606
MaeIII GTNAC 4 cut(s) 622, 1579, 1917, 1925
MalI GATC 5 cut(s) 153, 383, 978, 1470, 1755
MbiI CCGCTC 2 cut(s) 363, 816
MboI GATC 5 cut(s) 151, 381, 976, 1468, 1753
MboII GAAGA 6 cut(s) 38, 964, 971, 977, 1012, 1288
MfeI CAATTG 1 cut(s) 1118
MflI RGATCY 2 cut(s) 976, 1753
MhlI GDGCHC 3 cut(s) 252, 807, 1972
MlsI TGGCCA 1 cut(s) 1704
MluCI AATT 7 cut(s) 712, 1118, 1171, 1212, 1423, 1493, 1683
MluNI TGGCCA 1 cut(s) 1704
MlyI GAGTC 3 cut(s) 83, 446, 1464
MmeI TCCRAC 3 cut(s) 333, 423, 1720
Mox20I TGGCCA 1 cut(s) 1704
Mph1103I ATGCAT 1 cut(s) 882
MroNI GCCGGC 1 cut(s) 2201
MscI TGGCCA 1 cut(s) 1704
MseI TTAA 2 cut(s) 1407, 1536
MslI CAYNNNNRTG 3 cut(s) 1227, 1487, 2039
Msp20I TGGCCA 1 cut(s) 1704
MspA1I CMGCKG 1 cut(s) 2096
MspI CCGG 7 cut(s) 334, 385, 485, 643, 668, 800, 2202
MspR9I CCNGG 4 cut(s) 334, 335, 386, 590
MunI CAATTG 1 cut(s) 1118
Mva1269I GAATGC 2 cut(s) 864, 939
MvaI CCWGG 1 cut(s) 590
MvnI CGCG 3 cut(s) 84, 473, 602
NaeI GCCGGC 1 cut(s) 2203
NciI CCSGG 3 cut(s) 334, 335, 386
NcoI CCATGG 2 cut(s) 1475, 2034
NdeI CATATG 1 cut(s) 1488
NdeII GATC 5 cut(s) 151, 381, 976, 1468, 1753
NgoMIV GCCGGC 1 cut(s) 2201
NlaIV GGNNCC 3 cut(s) 368, 804, 1338
NmeAIII GCCGAG 1 cut(s) 888
NmuCI GTSAC 2 cut(s) 1579, 1917
NsbI TGCGCA 1 cut(s) 1529
NsiI ATGCAT 1 cut(s) 882
NspI RCATGY 3 cut(s) 1284, 1333, 1894
PaeI GCATGC 1 cut(s) 1894
PaeR7I CTCGAG 1 cut(s) 461
PceI AGGCCT 2 cut(s) 479, 886
PciI ACATGT 1 cut(s) 1329
PctI GAATGC 2 cut(s) 864, 939
PdiI GCCGGC 1 cut(s) 2203
PfeI GAWTC 5 cut(s) 338, 532, 683, 1307, 2045
PfoI TCCNGGA 1 cut(s) 588
PleI GAGTC 3 cut(s) 82, 445, 1463
PpsI GAGTC 3 cut(s) 82, 445, 1463
PpuMI RGGWCCY 1 cut(s) 166
PscI ACATGT 1 cut(s) 1329
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 588
PspEI GGTNACC 1 cut(s) 1925
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 3 cut(s) 368, 804, 1338
PspPI GGNCC 6 cut(s) 166, 387, 782, 797, 1048, 2144
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 1 cut(s) 2143
PstNI CAGNNNCTG 1 cut(s) 2138
PsuI RGATCY 2 cut(s) 976, 1753
PvuII CAGCTG 1 cut(s) 2096
RsaI GTAC 3 cut(s) 599, 1293, 1835
RsaNI GTAC 3 cut(s) 598, 1292, 1834
RseI CAYNNNNRTG 3 cut(s) 1227, 1487, 2039
SalI GTCGAC 1 cut(s) 1158
SaqAI TTAA 2 cut(s) 1407, 1536
Sau3AI GATC 5 cut(s) 151, 381, 976, 1468, 1753
Sau96I GGNCC 6 cut(s) 166, 387, 782, 797, 1048, 2144
SbfI CCTGCAGG 1 cut(s) 2143
SchI GAGTC 3 cut(s) 83, 446, 1464
ScrFI CCNGG 4 cut(s) 334, 335, 386, 590
SdaI CCTGCAGG 1 cut(s) 2143
SduI GDGCHC 3 cut(s) 252, 807, 1972
SfaNI GCATC 1 cut(s) 2140
SfcI CTRYAG 1 cut(s) 2139
Sfr274I CTCGAG 1 cut(s) 461
SinI GGWCC 2 cut(s) 166, 1048
SlaI CTCGAG 1 cut(s) 461
SmaI CCCGGG 1 cut(s) 335
SmiMI CAYNNNNRTG 3 cut(s) 1227, 1487, 2039
SmlI CTYRAG 4 cut(s) 161, 410, 461, 980
SmoI CTYRAG 4 cut(s) 161, 410, 461, 980
SpeI ACTAGT 1 cut(s) 725
SphI GCATGC 1 cut(s) 1894
Sse8387I CCTGCAGG 1 cut(s) 2143
Sse9I AATT 7 cut(s) 712, 1118, 1171, 1212, 1423, 1493, 1683
SseBI AGGCCT 2 cut(s) 479, 886
SspI AATATT 2 cut(s) 1370, 1774
SspMI CTAG 4 cut(s) 648, 726, 1256, 2105
StuI AGGCCT 2 cut(s) 479, 886
StyD4I CCNGG 4 cut(s) 332, 333, 384, 588
StyI CCWWGG 3 cut(s) 1475, 1859, 2034
TaaI ACNGT 7 cut(s) 628, 1157, 1247, 1412, 1449, 1881, 1974
TaiI ACGT 3 cut(s) 1165, 1591, 1609
TaqII GACCGA 1 cut(s) 482
TasI AATT 7 cut(s) 712, 1118, 1171, 1212, 1423, 1493, 1683
TatI WGTACW 1 cut(s) 1291
TauI GCSGC 4 cut(s) 21, 582, 826, 1269
TfiI GAWTC 5 cut(s) 338, 532, 683, 1307, 2045
Tru1I TTAA 2 cut(s) 1407, 1536
Tru9I TTAA 2 cut(s) 1407, 1536
TscAI CASTG 3 cut(s) 1588, 1705, 1712
TseFI GTSAC 2 cut(s) 1579, 1917
TseI GCWGC 8 cut(s) 448, 457, 502, 1982, 2005, 2054, 2096, 2117
Tsp45I GTSAC 2 cut(s) 1579, 1917
TspDTI ATGAA 8 cut(s) 671, 965, 986, 1187, 1289, 1475, 1536, 1971
TspGWI ACGGA 1 cut(s) 624
TspMI CCCGGG 1 cut(s) 333
TspRI CASTG 3 cut(s) 1588, 1705, 1712
VpaK11BI GGWCC 2 cut(s) 166, 1048
XapI RAATTY 1 cut(s) 712
XbaI TCTAGA 1 cut(s) 1255
XceI RCATGY 3 cut(s) 1284, 1333, 1894
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 461
XmaI CCCGGG 1 cut(s) 333
XmiI GTMKAC 2 cut(s) 1159, 2180
XspI CTAG 4 cut(s) 648, 726, 1256, 2105
ZraI GACGTC 1 cut(s) 1163
Zsp2I ATGCAT 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.