Rroxscaffold_4G00286950
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013734
Physical Location & Seq
Forward (+)
7901912 .. 7902778
867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286950.1

Sequence Viewer

Length: 474 bp
ATGGATATATCAGACAATAAGCTTAGTGGAGATATACCATCACAGGCCATTGGTCTGTCCTCCTCTGTCTTGCTCAACTTATCGCAAAACTCGCTAACTGGCATTTTGCCTGTGGAAGTGGGGAACCACTTTCAAGGAATCATTCCTTCTTCTTTGGCTGCTTTGAGAGGTCTTCAGTATCTAGATCTTTCACGAAACAACTTGTCAGGACATATTCCAAAAGACCTACAGAGGCTTCCATTCTTGATCCATTTGAACCTCTCGTTCAATAATTTGGAGGGTGAGACACTTCACCAAGCTACTGATGGATTCTCTCCGAGCAATCAAATTGGCTCAGGTGGTTTTGGCTCTGTATACAAAGGGATTCTTGATCAAGAAGAAAACAAAATTGTTGCCATAAAGGTCCTTAACCTTCAACAGAAAGGAGCTTCCAAGGGTTTTGTGGCGGAATGCAATGCACTGGGAAATATCTGA

Protein Analysis

157

Amino Acids

16.71

Weight (kDa)

5.72

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 41 - 92 2e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 354
AciI CCGC 1 cut(s) 446
AclWI GGATC 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 158
AgsI TTSAA 4 cut(s) 134, 256, 268, 416
AluBI AGCT 3 cut(s) 22, 299, 428
AluI AGCT 3 cut(s) 22, 299, 428
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 1 cut(s) 241
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 284, 293
AvaII GGWCC 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 145
BccI CCATC 2 cut(s) 46, 299
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 227
BglII AGATCT 1 cut(s) 184
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 125
BmrI ACTGGG 1 cut(s) 470
BmuI ACTGGG 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 164
Bpu10I CCTNAGC 1 cut(s) 334
BsaJI CCNNGG 1 cut(s) 432
Bse1I ACTGG 2 cut(s) 103, 465
Bse3DI GCAATG 1 cut(s) 460
BseDI CCNNGG 1 cut(s) 432
BseMI GCAATG 1 cut(s) 460
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 103, 465
BseRI GAGGAG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 145
BshFI GGCC 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 1 cut(s) 47
Bsp143I GATC 3 cut(s) 184, 246, 370
BspACI CCGC 1 cut(s) 446
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 347
BspLI GGNNCC 1 cut(s) 125
BspPI GGATC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 460
BsrI ACTGG 2 cut(s) 103, 465
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 3 cut(s) 184, 246, 370
BssNAI GTATAC 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 432
Bst1107I GTATAC 1 cut(s) 355
BstDEI CTNAG 2 cut(s) 23, 334
BstKTI GATC 3 cut(s) 187, 249, 373
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 3 cut(s) 184, 246, 370
BstMWI GCNNNNNNNGC 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BstZ17I GTATAC 1 cut(s) 355
BsuRI GGCC 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 458
Cfr13I GGNCC 1 cut(s) 403
CviJI RGCY 8 cut(s) 22, 47, 158, 235, 299, 333, 348, 428
CviKI_1 RGCY 8 cut(s) 22, 47, 158, 235, 299, 333, 348, 428
DdeI CTNAG 2 cut(s) 23, 334
DpnI GATC 3 cut(s) 186, 248, 372
DpnII GATC 3 cut(s) 184, 246, 370
EciI GGCGGA 1 cut(s) 461
Eco130I CCWWGG 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 432
FaiI YATR 5 cut(s) 8, 35, 213, 355, 398
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 182
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 3 cut(s) 138, 309, 364
HphI GGTGA 2 cut(s) 284, 293
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 3 cut(s) 13, 318, 473
Hpy188III TCNNGA 6 cut(s) 182, 192, 207, 244, 368, 374
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 156, 422
HpyCH4V TGCA 2 cut(s) 453, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
HpyF3I CTNAG 2 cut(s) 23, 334
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 3 cut(s) 184, 246, 370
LmnI GCTCC 1 cut(s) 425
LpnPI CCDG 6 cut(s) 29, 84, 123, 192, 321, 446
Lsp1109I GCAGC 1 cut(s) 145
MaeI CTAG 1 cut(s) 182
MalI GATC 3 cut(s) 186, 248, 372
MboI GATC 3 cut(s) 184, 246, 370
MboII GAAGA 3 cut(s) 141, 164, 389
MflI RGATCY 1 cut(s) 184
MluCI AATT 3 cut(s) 271, 327, 387
MnlI CCTC 6 cut(s) 70, 73, 161, 225, 269, 271
MseI TTAA 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 455
MwoI GCNNNNNNNGC 1 cut(s) 91
NdeII GATC 3 cut(s) 184, 246, 370
NlaIV GGNNCC 1 cut(s) 125
PctI GAATGC 1 cut(s) 455
PfeI GAWTC 3 cut(s) 138, 309, 364
PkrI GCNGC 1 cut(s) 160
PpuMI RGGWCCY 1 cut(s) 403
Psp5II RGGWCCY 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 125
PspPI GGNCC 1 cut(s) 403
PspPPI RGGWCCY 1 cut(s) 403
PsuI RGATCY 1 cut(s) 184
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 3 cut(s) 184, 246, 370
Sau96I GGNCC 1 cut(s) 403
SetI ASST 9 cut(s) 24, 172, 228, 261, 301, 340, 405, 414, 430
SfcI CTRYAG 1 cut(s) 227
SinI GGWCC 1 cut(s) 403
Sse9I AATT 3 cut(s) 271, 327, 387
SsiI CCGC 1 cut(s) 446
SspMI CTAG 1 cut(s) 182
StyI CCWWGG 1 cut(s) 432
TasI AATT 3 cut(s) 271, 327, 387
TfiI GAWTC 3 cut(s) 138, 309, 364
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 465
TseI GCWGC 1 cut(s) 158
TspRI CASTG 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 403
XbaI TCTAGA 1 cut(s) 181
XcmI CCANNNNNNNNNTGG 2 cut(s) 302, 439
XmiI GTMKAC 1 cut(s) 354
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.