RchiOBHm_Chr7g0204561

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004136
Physical Location & Seq
Reverse (-)
22240712 .. 22244864
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18310

Sequence Viewer

Length: 1128 bp
ATGTCTGTAATGGAGATGAGCTTGTTTCAATCGAAATCTCTGTCTGCTTCTGAGATGGCTCAGGTAGGGAGGATGTACTCAAATTCGTTTACGAGTTTCAGGTACTTACCGGCTGGTGTCAAAGTATCTCCACAGTTCTGTTCTTGCACAGGGAATCCTCTGAAGGCTTCTTCCTTTTCTCATCCTTCTAGTTCAAACAATAGATATCTGTCAACAAACGCCATTGGTAGTGTTGGAGGGGATAAACTTGCAGCTGAAACTTCCATATTTCAAATCTTTGGAACAAATGGTGCAAGGTCAAATCTATTTCCTAGAGACATTAGAGTACTTGATGCCTTTGATGATGAGTATGGGGGAGTTATCGTTGATCCAGAACGACTACCAGAAAATGCAGATGCCTTTGCTTACATCCTCCACTCCTCGCTTTCTAATTGGAAAATGGAGGGAAAGAAGGGAATTTGGCTTAAGCTGCCGGTAGAACGAGCTGAGCTTGTTCCAGTTGCTGTTAAGGAAGGTTTCCAATATCACCATGCAGAACGAGGATATGTGATGCTGACATATTGGATTCCAGAAGGACCATGCATGCTTCCCGCTAATGCTTCACATCAAGTAGGGGTTGGGGGATTTGTCATCAATGACAATAATGAGGTTCTTGTCGTACAAGAGAAACACTGTGCTCCTACATGCGTTGGTTTCTGGAAAATACCAACTGGCTTCATCCTTGAGTCGGAAGAGATTTTCACAGGAGCTGTGAGAGAAGTTAAGGAGGAAACTGGAATTGATACGGAGTTTGTGGAAGTTATAGCATTCAGGCATGCTCACAATGTTGCTTTCGAAAAGTCAGATTTGTTCTTTATCTGCATGCTAAAACCACTATCGACTCAGATTATAGTTGATGATCTTGAAATTGAAGCAGCCAAGTGGATGCCTCTGGCTGAGTTTGTGGAGCAACCACTAGTAAAAGAAGATTCCATGTTCAAGAAGGTCATTTCTATATGCATTGCCCGGCTAGGAAAGCATTACTGCGGTTTATCTTCTCATCAAGTGGTCTCCAAGTTCGATGGCAGGTTGTCTTCCTTGTACTATAACGTCGTTGATACACAAGATTTCAACTGTTCAAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009611 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

375

Amino Acids

41.71

Weight (kDa)

5.44

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nudix_hydro PF18290 111 - 189 8.7e-31 Nudix hydrolase domain
NUDIX PF00293 202 - 322 1.2e-19 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1056
AciI CCGC 2 cut(s) 591, 1026
AclWI GGATC 1 cut(s) 362
AcsI RAATTY 2 cut(s) 82, 456
AcuI CTGAAG 1 cut(s) 182
AfaI GTAC 5 cut(s) 77, 104, 327, 660, 1082
AfiI CCNNNNNNNGG 1 cut(s) 727
AflII CTTAAG 1 cut(s) 464
AgsI TTSAA 8 cut(s) 29, 195, 272, 905, 911, 979, 1111, 1119
AhlI ACTAGT 1 cut(s) 955
AluBI AGCT 6 cut(s) 21, 254, 469, 485, 490, 749
AluI AGCT 6 cut(s) 21, 254, 469, 485, 490, 749
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 2 cut(s) 309, 1054
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 2 cut(s) 503, 749
ApeKI GCWGC 3 cut(s) 251, 469, 914
ApoI RAATTY 2 cut(s) 82, 456
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspS9I GGNCC 1 cut(s) 575
AsuC2I CCSGG 1 cut(s) 1006
AsuHPI GGTGA 1 cut(s) 518
AsuII TTCGAA 1 cut(s) 834
AvaII GGWCC 1 cut(s) 575
BbsI GAAGAC 1 cut(s) 1065
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 3 cut(s) 263, 456, 926
BccI CCATC 2 cut(s) 49, 1055
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 2 cut(s) 309, 1054
BcuI ACTAGT 1 cut(s) 955
BfaI CTAG 4 cut(s) 189, 312, 956, 1010
BfrI CTTAAG 1 cut(s) 464
BfuAI ACCTGC 1 cut(s) 1056
BisI GCNGC 3 cut(s) 252, 470, 915
BlpI GCTNAGC 1 cut(s) 486
BlsI GCNGC 3 cut(s) 253, 471, 916
BmcAI AGTACT 1 cut(s) 327
Bme1390I CCNGG 1 cut(s) 1006
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 575
BmrFI CCNGG 1 cut(s) 1006
BmsI GCATC 4 cut(s) 322, 385, 540, 915
BpiI GAAGAC 1 cut(s) 1065
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 486
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 1 cut(s) 743
BpuMI CCSGG 1 cut(s) 1006
BsaI GGTCTC 1 cut(s) 1054
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 727
Bse118I RCCGGY 2 cut(s) 109, 472
Bse1I ACTGG 3 cut(s) 497, 715, 778
Bse3DI GCAATG 1 cut(s) 999
BseGI GGATG 5 cut(s) 78, 181, 408, 717, 930
BseLI CCNNNNNNNGG 1 cut(s) 727
BseMI GCAATG 1 cut(s) 999
BseMII CTCAG 5 cut(s) 42, 74, 477, 896, 927
BseNI ACTGG 3 cut(s) 497, 715, 778
BseRI GAGGAG 1 cut(s) 409
BseXI GCAGC 3 cut(s) 263, 456, 926
BsiHKAI GWGCWC 1 cut(s) 679
BsiSI CCGG 3 cut(s) 110, 473, 1006
BslI CCNNNNNNNGG 1 cut(s) 727
BsmAI GTCTC 2 cut(s) 309, 1054
BsmI GAATGC 1 cut(s) 806
Bso31I GGTCTC 1 cut(s) 1054
Bsp119I TTCGAA 1 cut(s) 834
Bsp1286I GDGCHC 1 cut(s) 679
Bsp143I GATC 2 cut(s) 367, 898
Bsp1720I GCTNAGC 1 cut(s) 486
BspACI CCGC 2 cut(s) 591, 1026
BspCNI CTCAG 5 cut(s) 43, 73, 478, 895, 928
BspMI ACCTGC 1 cut(s) 1056
BspPI GGATC 1 cut(s) 362
BspT104I TTCGAA 1 cut(s) 834
BspTI CTTAAG 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 1054
BsrDI GCAATG 1 cut(s) 999
BsrFI RCCGGY 2 cut(s) 109, 472
BsrI ACTGG 3 cut(s) 497, 715, 778
BssAI RCCGGY 2 cut(s) 109, 472
BssMI GATC 2 cut(s) 367, 898
Bst4CI ACNGT 3 cut(s) 135, 674, 1115
Bst6I CTCTTC 1 cut(s) 726
BstAFI CTTAAG 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 3 cut(s) 584, 816, 863
BstDEI CTNAG 5 cut(s) 51, 60, 486, 882, 936
BstF5I GGATG 5 cut(s) 78, 181, 408, 717, 930
BstKTI GATC 2 cut(s) 370, 901
BstMAI GTCTC 2 cut(s) 309, 1054
BstMBI GATC 2 cut(s) 367, 898
BstMWI GCNNNNNNNGC 2 cut(s) 469, 1015
BstNSI RCATGY 4 cut(s) 586, 687, 818, 865
BstSCI CCNGG 1 cut(s) 1004
BstV1I GCAGC 3 cut(s) 263, 456, 926
BstV2I GAAGAC 1 cut(s) 1065
BtsCI GGATG 5 cut(s) 78, 181, 408, 717, 930
BtsIMutI CAGTG 1 cut(s) 670
BveI ACCTGC 1 cut(s) 1056
Cac8I GCNNGC 3 cut(s) 584, 816, 863
CaiI CAGNNNCTG 2 cut(s) 503, 749
Cfr10I RCCGGY 2 cut(s) 109, 472
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 5 cut(s) 76, 103, 326, 659, 1081
CviAII CATG 7 cut(s) 530, 579, 583, 684, 815, 862, 973
CviQI GTAC 5 cut(s) 76, 103, 326, 659, 1081
DdeI CTNAG 5 cut(s) 51, 60, 486, 882, 936
DpnI GATC 2 cut(s) 369, 900
DpnII GATC 2 cut(s) 367, 898
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Eco31I GGTCTC 1 cut(s) 1054
Eco32I GATATC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 182
EcoRV GATATC 1 cut(s) 206
EcoT22I ATGCAT 2 cut(s) 584, 1001
FaeI CATG 7 cut(s) 533, 582, 586, 687, 818, 865, 976
FatI CATG 7 cut(s) 529, 578, 582, 683, 814, 861, 972
FauI CCCGC 1 cut(s) 598
Fnu4HI GCNGC 3 cut(s) 252, 470, 915
FokI GGATG 5 cut(s) 85, 168, 395, 704, 937
Fsp4HI GCNGC 3 cut(s) 252, 470, 915
FspBI CTAG 4 cut(s) 189, 312, 956, 1010
GluI GCNGC 3 cut(s) 252, 470, 915
HapII CCGG 3 cut(s) 110, 473, 1006
Hin1II CATG 7 cut(s) 533, 582, 586, 687, 818, 865, 976
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 5 cut(s) 154, 565, 725, 880, 968
HpaII CCGG 3 cut(s) 110, 473, 1006
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 2 cut(s) 90, 213
Hpy188I TCNGA 5 cut(s) 52, 162, 730, 844, 885
Hpy188III TCNNGA 5 cut(s) 371, 569, 697, 902, 979
Hpy8I GTNNAC 2 cut(s) 90, 213
Hpy99I CGWCG 1 cut(s) 1094
HpyAV CCTTC 6 cut(s) 157, 195, 445, 506, 566, 976
HpyCH4III ACNGT 3 cut(s) 135, 674, 1115
HpyCH4IV ACGT 1 cut(s) 1089
HpyCH4V TGCA 8 cut(s) 147, 251, 293, 392, 533, 582, 861, 999
HpyF10VI GCNNNNNNNGC 2 cut(s) 469, 1015
HpyF3I CTNAG 5 cut(s) 51, 60, 486, 882, 936
HpySE526I ACGT 1 cut(s) 1089
Hsp92II CATG 7 cut(s) 533, 582, 586, 687, 818, 865, 976
Kzo9I GATC 2 cut(s) 367, 898
LmnI GCTCC 3 cut(s) 682, 746, 946
Lsp1109I GCAGC 3 cut(s) 263, 456, 926
LweI GCATC 4 cut(s) 322, 385, 540, 915
MaeI CTAG 4 cut(s) 189, 312, 956, 1010
MaeII ACGT 1 cut(s) 1089
MalI GATC 2 cut(s) 369, 900
MboI GATC 2 cut(s) 367, 898
MboII GAAGA 5 cut(s) 162, 743, 977, 1026, 1065
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 6 cut(s) 82, 430, 456, 777, 906, 1120
MlyI GAGTC 2 cut(s) 734, 874
MmeI TCCRAC 2 cut(s) 214, 708
Mph1103I ATGCAT 2 cut(s) 584, 1001
MseI TTAA 3 cut(s) 465, 507, 762
MspA1I CMGCKG 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 3 cut(s) 110, 473, 1006
MspR9I CCNGG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 806
MwoI GCNNNNNNNGC 2 cut(s) 469, 1015
NciI CCSGG 1 cut(s) 1006
NdeII GATC 2 cut(s) 367, 898
NlaIII CATG 7 cut(s) 533, 582, 586, 687, 818, 865, 976
NsiI ATGCAT 2 cut(s) 584, 1001
NspI RCATGY 4 cut(s) 586, 687, 818, 865
NspV TTCGAA 1 cut(s) 834
PaeI GCATGC 3 cut(s) 586, 818, 865
PctI GAATGC 1 cut(s) 806
PfeI GAWTC 3 cut(s) 154, 565, 968
PkrI GCNGC 3 cut(s) 253, 471, 916
PleI GAGTC 2 cut(s) 733, 874
PpsI GAGTC 2 cut(s) 733, 874
PspPI GGNCC 1 cut(s) 575
PstNI CAGNNNCTG 2 cut(s) 503, 749
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 5 cut(s) 77, 104, 327, 660, 1082
RsaNI GTAC 5 cut(s) 76, 103, 326, 659, 1081
SaqAI TTAA 3 cut(s) 465, 507, 762
SatI GCNGC 3 cut(s) 252, 470, 915
Sau3AI GATC 2 cut(s) 367, 898
Sau96I GGNCC 1 cut(s) 575
ScaI AGTACT 1 cut(s) 327
SchI GAGTC 2 cut(s) 734, 874
ScrFI CCNGG 1 cut(s) 1006
SduI GDGCHC 1 cut(s) 679
SfaNI GCATC 4 cut(s) 322, 385, 540, 915
SfuI TTCGAA 1 cut(s) 834
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 464, 722
SmoI CTYRAG 2 cut(s) 464, 722
SpeI ACTAGT 1 cut(s) 955
SphI GCATGC 3 cut(s) 586, 818, 865
Sse9I AATT 6 cut(s) 82, 430, 456, 777, 906, 1120
SsiI CCGC 2 cut(s) 591, 1026
SspMI CTAG 4 cut(s) 189, 312, 956, 1010
StyD4I CCNGG 1 cut(s) 1004
TaaI ACNGT 3 cut(s) 135, 674, 1115
TaiI ACGT 1 cut(s) 1092
TaqI TCGA 4 cut(s) 32, 834, 878, 1059
TasI AATT 6 cut(s) 82, 430, 456, 777, 906, 1120
TatI WGTACW 3 cut(s) 75, 325, 1080
TfiI GAWTC 3 cut(s) 154, 565, 968
Tru1I TTAA 3 cut(s) 465, 507, 762
Tru9I TTAA 3 cut(s) 465, 507, 762
TscAI CASTG 1 cut(s) 677
TseI GCWGC 3 cut(s) 251, 469, 914
TspDTI ATGAA 1 cut(s) 706
TspGWI ACGGA 1 cut(s) 800
TspRI CASTG 1 cut(s) 677
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 2 cut(s) 82, 456
XceI RCATGY 4 cut(s) 586, 687, 818, 865
XspI CTAG 4 cut(s) 189, 312, 956, 1010
ZrmI AGTACT 1 cut(s) 327
Zsp2I ATGCAT 2 cut(s) 584, 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.