Rw7G018610

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004136
Physical Location & Seq
Reverse (-)
20171265 .. 20175399
4135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018610.1

Sequence Viewer

Length: 801 bp
ATGTCTGTAATGGAGATGAGCTTGTTTCAATCGAAATCTCTGTCTGCTTCTGAGATGGCTCAGGTAGGGAGGATGTACTCAAATTCGTTTACGAGTTTCAGGTACTTAACTGCTGGTGTCAAAGTATCTTCACAGTTCTGTTCTTGCACAGGGAATCCTCTGAAGGCTTCTTCCTGTTCTCATCCTTCTAGTTCAAACAATAGATATCTGTCAACAAACGCCATTGGTAGTGTTGGAGGGGATAAACTTGCAGCTGAAACTTCCTTATTTCAAATCTTTGGAACAAATGGTGCAAGGTCAAATCTGTTTCCTAGAGACATTAGAGTACTTGATGCCTTTGATGATGAGTATGGGGGAGTTATCGTTGATCCAGAACGACTACCAGAAAATGCAGATGCCTTTGCTTACATCCTCCACTCCTCGCTTTCTAATTGGAAAATGGAGGGGAAGAAGGGAATTTGGCTTAAGCTGCCGGTAGAACGAGCTGAGCTTGTTCCAGTTGCTGTTAAGGAAGGTTTCCAATATCACCATGCAGAACGAGGATATGTGATGCTGACATATTGGATTCCAGAAGGACCATGCATGCTTCCCGCTAATGCTTCACATCAAGTAGGGGTTGGGGGATTTGTCATCAATGACAATAATGAGGTTCTTGTCGTACAAGAGAAACACTGTGCTCCTACATGCGTTGGTTTCTGGAAAATACCAACTGGCTTCATCCTTGAGTCGGAAGAGATTTTCACAGGAGCTGTGAGAGAAGTTAAGGAGGAAACTGGAGTAAGAATGTGTATTTATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009611 GO:0044424 GO:0044444 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

266

Amino Acids

29.33

Weight (kDa)

5.72

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nudix_hydro PF18290 111 - 189 4.7e-31 Nudix hydrolase domain
NUDIX PF00293 202 - 261 2.1e-11 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 591
AclWI GGATC 1 cut(s) 362
AcsI RAATTY 2 cut(s) 82, 456
AcuI CTGAAG 1 cut(s) 182
AfaI GTAC 4 cut(s) 77, 104, 327, 660
AfiI CCNNNNNNNGG 1 cut(s) 727
AflII CTTAAG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 29, 195, 272
AluBI AGCT 6 cut(s) 21, 254, 469, 485, 490, 749
AluI AGCT 6 cut(s) 21, 254, 469, 485, 490, 749
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 2 cut(s) 503, 749
ApeKI GCWGC 2 cut(s) 251, 469
ApoI RAATTY 2 cut(s) 82, 456
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 518
AvaII GGWCC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 2 cut(s) 263, 456
BccI CCATC 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 309
BfaI CTAG 2 cut(s) 189, 312
BfrI CTTAAG 1 cut(s) 464
BisI GCNGC 2 cut(s) 252, 470
BlpI GCTNAGC 1 cut(s) 486
BlsI GCNGC 2 cut(s) 253, 471
BmcAI AGTACT 1 cut(s) 327
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 575
BmsI GCATC 3 cut(s) 322, 385, 540
BpmI CTGGAG 1 cut(s) 795
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 743
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 727
Bse118I RCCGGY 1 cut(s) 472
Bse1I ACTGG 3 cut(s) 497, 715, 778
BseGI GGATG 4 cut(s) 78, 181, 408, 717
BseLI CCNNNNNNNGG 1 cut(s) 727
BseMII CTCAG 3 cut(s) 42, 74, 477
BseNI ACTGG 3 cut(s) 497, 715, 778
BseRI GAGGAG 1 cut(s) 409
BseXI GCAGC 2 cut(s) 263, 456
BsiHKAI GWGCWC 1 cut(s) 679
BsiSI CCGG 1 cut(s) 473
BslI CCNNNNNNNGG 1 cut(s) 727
BsmAI GTCTC 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 679
Bsp143I GATC 1 cut(s) 367
Bsp1720I GCTNAGC 1 cut(s) 486
BspACI CCGC 1 cut(s) 591
BspCNI CTCAG 3 cut(s) 43, 73, 478
BspPI GGATC 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 472
BsrI ACTGG 3 cut(s) 497, 715, 778
BssAI RCCGGY 1 cut(s) 472
BssMI GATC 1 cut(s) 367
Bst4CI ACNGT 2 cut(s) 135, 674
Bst6I CTCTTC 1 cut(s) 726
BstAFI CTTAAG 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 584
BstDEI CTNAG 3 cut(s) 51, 60, 486
BstF5I GGATG 4 cut(s) 78, 181, 408, 717
BstKTI GATC 1 cut(s) 370
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 469
BstNSI RCATGY 2 cut(s) 586, 687
BstV1I GCAGC 2 cut(s) 263, 456
BtsCI GGATG 4 cut(s) 78, 181, 408, 717
BtsIMutI CAGTG 1 cut(s) 670
Cac8I GCNNGC 1 cut(s) 584
CaiI CAGNNNCTG 2 cut(s) 503, 749
Cfr10I RCCGGY 1 cut(s) 472
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 4 cut(s) 76, 103, 326, 659
CviAII CATG 4 cut(s) 530, 579, 583, 684
CviQI GTAC 4 cut(s) 76, 103, 326, 659
DdeI CTNAG 3 cut(s) 51, 60, 486
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Eco32I GATATC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 182
EcoRV GATATC 1 cut(s) 206
EcoT22I ATGCAT 1 cut(s) 584
FaeI CATG 4 cut(s) 533, 582, 586, 687
FaiI YATR 7 cut(s) 351, 531, 546, 559, 580, 584, 685
FatI CATG 4 cut(s) 529, 578, 582, 683
FauI CCCGC 1 cut(s) 598
Fnu4HI GCNGC 2 cut(s) 252, 470
FokI GGATG 4 cut(s) 85, 168, 395, 704
Fsp4HI GCNGC 2 cut(s) 252, 470
FspBI CTAG 2 cut(s) 189, 312
GluI GCNGC 2 cut(s) 252, 470
GsuI CTGGAG 1 cut(s) 795
HapII CCGG 1 cut(s) 473
Hin1II CATG 4 cut(s) 533, 582, 586, 687
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 3 cut(s) 154, 565, 725
HpaII CCGG 1 cut(s) 473
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 2 cut(s) 90, 213
Hpy188I TCNGA 3 cut(s) 52, 162, 730
Hpy188III TCNNGA 3 cut(s) 371, 569, 697
Hpy8I GTNNAC 2 cut(s) 90, 213
HpyAV CCTTC 5 cut(s) 157, 195, 445, 506, 566
HpyCH4III ACNGT 2 cut(s) 135, 674
HpyCH4V TGCA 6 cut(s) 147, 251, 293, 392, 533, 582
HpyF10VI GCNNNNNNNGC 1 cut(s) 469
HpyF3I CTNAG 3 cut(s) 51, 60, 486
Hsp92II CATG 4 cut(s) 533, 582, 586, 687
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 682, 746
Lsp1109I GCAGC 2 cut(s) 263, 456
LweI GCATC 3 cut(s) 322, 385, 540
MaeI CTAG 2 cut(s) 189, 312
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 4 cut(s) 120, 162, 460, 743
MhlI GDGCHC 1 cut(s) 679
MluCI AATT 3 cut(s) 82, 430, 456
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 214, 708
MnlI CCTC 9 cut(s) 63, 168, 230, 422, 430, 436, 533, 640, 760
Mph1103I ATGCAT 1 cut(s) 584
MseI TTAA 4 cut(s) 107, 465, 507, 762
MspA1I CMGCKG 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 1 cut(s) 473
MwoI GCNNNNNNNGC 1 cut(s) 469
NdeII GATC 1 cut(s) 367
NlaIII CATG 4 cut(s) 533, 582, 586, 687
NsiI ATGCAT 1 cut(s) 584
NspI RCATGY 2 cut(s) 586, 687
PaeI GCATGC 1 cut(s) 586
PfeI GAWTC 2 cut(s) 154, 565
PkrI GCNGC 2 cut(s) 253, 471
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PspPI GGNCC 1 cut(s) 575
PstNI CAGNNNCTG 2 cut(s) 503, 749
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 4 cut(s) 77, 104, 327, 660
RsaNI GTAC 4 cut(s) 76, 103, 326, 659
SaqAI TTAA 4 cut(s) 107, 465, 507, 762
SatI GCNGC 2 cut(s) 252, 470
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 1 cut(s) 575
ScaI AGTACT 1 cut(s) 327
SchI GAGTC 1 cut(s) 734
SduI GDGCHC 1 cut(s) 679
SfaNI GCATC 3 cut(s) 322, 385, 540
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 464, 722
SmoI CTYRAG 2 cut(s) 464, 722
SphI GCATGC 1 cut(s) 586
Sse9I AATT 3 cut(s) 82, 430, 456
SsiI CCGC 1 cut(s) 591
SspMI CTAG 2 cut(s) 189, 312
TaaI ACNGT 2 cut(s) 135, 674
TaqI TCGA 1 cut(s) 32
TasI AATT 3 cut(s) 82, 430, 456
TatI WGTACW 2 cut(s) 75, 325
TfiI GAWTC 2 cut(s) 154, 565
Tru1I TTAA 4 cut(s) 107, 465, 507, 762
Tru9I TTAA 4 cut(s) 107, 465, 507, 762
TscAI CASTG 1 cut(s) 677
TseI GCWGC 2 cut(s) 251, 469
TspDTI ATGAA 1 cut(s) 706
TspRI CASTG 1 cut(s) 677
Vha464I CTTAAG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 2 cut(s) 82, 456
XceI RCATGY 2 cut(s) 586, 687
XspI CTAG 2 cut(s) 189, 312
ZrmI AGTACT 1 cut(s) 327
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.