RCHIOBHM_MTG0498791

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0005840
Physical Location & Seq
Reverse (-)
173808 .. 174715
908 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15638

Sequence Viewer

Length: 762 bp
ATGACACGATGGCTGTTCTCCACTAACCACAAGGATATAGGGACTCTATATTTCATCTTCGGTGCTATTGCTGGAGTGATGGGCACATGCTTCTCAGTACTGATTCGTATGGAATTAGCACGACCCGGCGATCAAATTCTTGGTGGGAATCATCAACTTTATAATGTTTTAATAACGGCTCATGCTTTTTTAATGATCTTTTTTATGGTTATGCCGGCGATGATAGGTGGATTTGGTAATTGGTTTGTTCCGATTCTGATAGGTGCACCTGACATGGCATTTCCACGATTAAATAATATTTCATTCTGGTTGTTGCCACCAAGTCTCTTGCTCCTATTAAGCTCAGCCTTAGTAGAAGTGGGTAGCGGGACTGGGTGGACGGTCTATCCGCCCTTAAGTGGTATTACCAGCCATTCTGGGGGAGCTGTTGATTTAGCAATTTTTAGTCTTCATCTATCTGGTATTTCATCCATTTTAGGTTCTATCAATTTTATAACAACTATCTTCAACATGCGTGGACCTGGAATGACTATGCATAGATTACCCCTATTTGTGTGGTCCGTTCTAGTGACAGCATTCCTACTTTTATTATCACTTCCGGTACTGGCAGGGGCAATTACCATGTTATTAACCGATCGAAACTTTAATACAACCTTTTTTGATCCCGCTGGGGGGGAGACCCCATATTATACCAGCATCTCTTTTGGTTTTTCGGTCATCCAGAGGTGTATATTCTCATTCTGCCTGGATTCGGTATCATAA

Protein Analysis

253

Amino Acids

27.56

Weight (kDa)

6.57

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX1 PF00115 12 - 225 3.4e-61 Cytochrome C and Quinol oxidase polypeptide I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 162, 494
AciI CCGC 3 cut(s) 366, 389, 666
AclWI GGATC 1 cut(s) 656
AcsI RAATTY 1 cut(s) 135
AfaI GTAC 2 cut(s) 99, 603
AfiI CCNNNNNNNGG 5 cut(s) 398, 418, 671, 672, 751
AflII CTTAAG 1 cut(s) 394
AgsI TTSAA 1 cut(s) 508
AjnI CCWGG 2 cut(s) 520, 744
AluBI AGCT 2 cut(s) 342, 425
AluI AGCT 2 cut(s) 342, 425
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 2 cut(s) 329, 671
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 1 cut(s) 656
ApaLI GTGCAC 1 cut(s) 264
ApoI RAATTY 1 cut(s) 135
AspS9I GGNCC 2 cut(s) 518, 558
AsuC2I CCSGG 1 cut(s) 126
AvaII GGWCC 2 cut(s) 518, 558
BaeGI GKGCMC 2 cut(s) 86, 268
BbsI GAAGAC 1 cut(s) 440
Bbv12I GWGCWC 1 cut(s) 268
BccI CCATC 2 cut(s) 3, 73
BceAI ACGGC 1 cut(s) 192
BciT130I CCWGG 2 cut(s) 522, 746
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 329, 671
BfaI CTAG 1 cut(s) 566
BfrI CTTAAG 1 cut(s) 394
BlpI GCTNAGC 1 cut(s) 343
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 3 cut(s) 126, 522, 746
Bme18I GGWCC 2 cut(s) 518, 558
BmgT120I GGNCC 2 cut(s) 518, 558
BmrFI CCNGG 3 cut(s) 126, 522, 746
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 440
BpmI CTGGAG 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 343
BpuMI CCSGG 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 671
BsaWI WCCGGW 1 cut(s) 598
Bsc4I CCNNNNNNNGG 5 cut(s) 398, 418, 671, 672, 751
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 376, 609
BseBI CCWGG 2 cut(s) 522, 746
BseGI GGATG 2 cut(s) 467, 717
BseLI CCNNNNNNNGG 5 cut(s) 398, 418, 671, 672, 751
BseMII CTCAG 2 cut(s) 108, 357
BseNI ACTGG 2 cut(s) 376, 609
BseSI GKGCMC 2 cut(s) 86, 268
BseYI CCCAGC 1 cut(s) 668
Bsh1285I CGRYCG 1 cut(s) 637
BsiEI CGRYCG 1 cut(s) 637
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 3 cut(s) 126, 215, 599
BslFI GGGAC 2 cut(s) 55, 382
BslI CCNNNNNNNGG 5 cut(s) 398, 418, 671, 672, 751
BsmAI GTCTC 2 cut(s) 329, 671
BsmFI GGGAC 2 cut(s) 55, 382
BsmI GAATGC 1 cut(s) 575
Bso31I GGTCTC 1 cut(s) 671
Bsp1286I GDGCHC 2 cut(s) 86, 268
Bsp143I GATC 4 cut(s) 130, 195, 634, 661
Bsp1720I GCTNAGC 1 cut(s) 343
BspACI CCGC 3 cut(s) 366, 389, 666
BspCNI CTCAG 2 cut(s) 107, 356
BspPI GGATC 1 cut(s) 656
BspTI CTTAAG 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 671
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 2 cut(s) 376, 609
BssAI RCCGGY 1 cut(s) 214
BssMI GATC 4 cut(s) 130, 195, 634, 661
Bst2UI CCWGG 2 cut(s) 522, 746
Bst4CI ACNGT 1 cut(s) 382
BstAFI CTTAAG 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 3 cut(s) 94, 343, 349
BstF5I GGATG 2 cut(s) 467, 717
BstKTI GATC 4 cut(s) 133, 198, 637, 664
BstMAI GTCTC 2 cut(s) 329, 671
BstMBI GATC 4 cut(s) 130, 195, 634, 661
BstMCI CGRYCG 1 cut(s) 637
BstNI CCWGG 2 cut(s) 522, 746
BstNSI RCATGY 2 cut(s) 90, 514
BstSCI CCNGG 3 cut(s) 124, 520, 744
BstSLI GKGCMC 2 cut(s) 86, 268
BstV2I GAAGAC 1 cut(s) 440
BtgZI GCGATG 1 cut(s) 233
BtsCI GGATG 2 cut(s) 467, 717
Cac8I GCNNGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 518, 558
Csp6I GTAC 2 cut(s) 98, 602
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviAII CATG 5 cut(s) 87, 182, 274, 511, 622
CviJI RGCY 6 cut(s) 13, 179, 342, 347, 411, 425
CviKI_1 RGCY 6 cut(s) 13, 179, 342, 347, 411, 425
CviQI GTAC 2 cut(s) 98, 602
DdeI CTNAG 3 cut(s) 94, 343, 349
DpnI GATC 4 cut(s) 132, 197, 636, 663
DpnII GATC 4 cut(s) 130, 195, 634, 661
EciI GGCGGA 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 671
Eco47I GGWCC 2 cut(s) 518, 558
EcoRII CCWGG 2 cut(s) 520, 744
EcoT22I ATGCAT 1 cut(s) 537
FaeI CATG 5 cut(s) 90, 185, 277, 514, 625
FaqI GGGAC 2 cut(s) 55, 382
FatI CATG 5 cut(s) 86, 181, 273, 510, 621
FauI CCCGC 2 cut(s) 359, 673
FokI GGATG 2 cut(s) 454, 704
FspBI CTAG 1 cut(s) 566
GsaI CCCAGC 1 cut(s) 672
GsuI CTGGAG 1 cut(s) 93
HapII CCGG 3 cut(s) 126, 215, 599
Hin1II CATG 5 cut(s) 90, 185, 277, 514, 625
HinfI GANTC 5 cut(s) 43, 103, 148, 253, 749
HpaII CCGG 3 cut(s) 126, 215, 599
Hpy166II GTNNAC 3 cut(s) 266, 378, 518
Hpy188I TCNGA 2 cut(s) 252, 258
Hpy188III TCNNGA 1 cut(s) 721
Hpy8I GTNNAC 3 cut(s) 266, 378, 518
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4V TGCA 2 cut(s) 266, 535
HpyF3I CTNAG 3 cut(s) 94, 343, 349
Hsp92II CATG 5 cut(s) 90, 185, 277, 514, 625
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 4 cut(s) 130, 195, 634, 661
LmnI GCTCC 2 cut(s) 336, 422
LweI GCATC 1 cut(s) 705
MaeI CTAG 1 cut(s) 566
MaeIII GTNAC 1 cut(s) 568
MalI GATC 4 cut(s) 132, 197, 636, 663
MboI GATC 4 cut(s) 130, 195, 634, 661
MboII GAAGA 3 cut(s) 49, 440, 496
MhlI GDGCHC 2 cut(s) 86, 268
MluCI AATT 6 cut(s) 113, 135, 238, 438, 487, 615
MlyI GAGTC 1 cut(s) 37
MnlI CCTC 1 cut(s) 717
Mph1103I ATGCAT 1 cut(s) 537
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 7 cut(s) 170, 191, 290, 338, 395, 629, 645
MspA1I CMGCKG 1 cut(s) 668
MspCI CTTAAG 1 cut(s) 394
MspI CCGG 3 cut(s) 126, 215, 599
MspR9I CCNGG 3 cut(s) 126, 522, 746
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 2 cut(s) 522, 746
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 126
NdeII GATC 4 cut(s) 130, 195, 634, 661
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 5 cut(s) 90, 185, 277, 514, 625
NmuCI GTSAC 1 cut(s) 568
NsiI ATGCAT 1 cut(s) 537
NspI RCATGY 2 cut(s) 90, 514
PctI GAATGC 1 cut(s) 575
PdiI GCCGGC 1 cut(s) 216
PfeI GAWTC 4 cut(s) 103, 148, 253, 749
Ple19I CGATCG 1 cut(s) 637
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
PsiI TTATAA 2 cut(s) 162, 494
Psp6I CCWGG 2 cut(s) 520, 744
PspFI CCCAGC 1 cut(s) 668
PspGI CCWGG 2 cut(s) 520, 744
PspPI GGNCC 2 cut(s) 518, 558
PvuI CGATCG 1 cut(s) 637
RsaI GTAC 2 cut(s) 99, 603
RsaNI GTAC 2 cut(s) 98, 602
SaqAI TTAA 7 cut(s) 170, 191, 290, 338, 395, 629, 645
Sau3AI GATC 4 cut(s) 130, 195, 634, 661
Sau96I GGNCC 2 cut(s) 518, 558
ScaI AGTACT 1 cut(s) 99
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 3 cut(s) 126, 522, 746
SduI GDGCHC 2 cut(s) 86, 268
SetI ASST 9 cut(s) 229, 265, 271, 344, 427, 481, 523, 656, 728
SfaNI GCATC 1 cut(s) 705
SinI GGWCC 2 cut(s) 518, 558
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 6 cut(s) 113, 135, 238, 438, 487, 615
SsiI CCGC 3 cut(s) 366, 389, 666
SspI AATATT 1 cut(s) 298
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 3 cut(s) 124, 520, 744
TaaI ACNGT 1 cut(s) 382
TaqI TCGA 1 cut(s) 637
TaqII GACCGA 1 cut(s) 703
TasI AATT 6 cut(s) 113, 135, 238, 438, 487, 615
TatI WGTACW 1 cut(s) 97
TfiI GAWTC 4 cut(s) 103, 148, 253, 749
Tru1I TTAA 7 cut(s) 170, 191, 290, 338, 395, 629, 645
Tru9I TTAA 7 cut(s) 170, 191, 290, 338, 395, 629, 645
TseFI GTSAC 1 cut(s) 568
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 4 cut(s) 43, 291, 440, 456
TspGWI ACGGA 1 cut(s) 550
Vha464I CTTAAG 1 cut(s) 394
VneI GTGCAC 1 cut(s) 264
VpaK11BI GGWCC 2 cut(s) 518, 558
XapI RAATTY 1 cut(s) 135
XceI RCATGY 2 cut(s) 90, 514
XspI CTAG 1 cut(s) 566
ZrmI AGTACT 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.