RLG00000003527

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014847
Physical Location & Seq
Reverse (-)
49649537 .. 49650481
945 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003527

Sequence Viewer

Length: 312 bp
ATGACGCCGTACGACGTCCCACCGCCATCACTGATTACAGCAAGAGGTTACTTCAGCACAAGGAGCTCAAATCCGGAGTCCGCGGAAGTCAGATTCGCACTCCAAATCCTCCTTATTCATGTTCGATTCTACATCTGCAACCGGAGAGCGTCTCTCTCACCATCGGCGAACCAGCTCACAAGCAATTCGAAAGTACAGAGAGCTTCATCTACGCTTGAAGAGTTGAGATATTATCACGACGATTCAGCTCTCGCAGGATCGAAAGCAATATACAAATTCAAAGGTCTGAGTGGTGGTGCTGGTGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

104

Amino Acids

11.27

Weight (kDa)

9.66

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 73
AciI CCGC 3 cut(s) 23, 81, 83
AclWI GGATC 1 cut(s) 265
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 37
AcyI GRCGYC 2 cut(s) 5, 15
AfaI GTAC 2 cut(s) 11, 195
AgsI TTSAA 2 cut(s) 218, 280
AluBI AGCT 4 cut(s) 66, 175, 203, 248
AluI AGCT 4 cut(s) 66, 175, 203, 248
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 1 cut(s) 265
Aor13HI TCCGGA 1 cut(s) 73
ApoI RAATTY 1 cut(s) 275
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
AsuHPI GGTGA 1 cut(s) 150
AsuII TTCGAA 1 cut(s) 188
BanII GRGCYC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 68
BccI CCATC 2 cut(s) 34, 169
BcoDI GTCTC 1 cut(s) 156
BplI GAGNNNNNCTC 4 cut(s) 136, 168, 138, 170
Bpu14I TTCGAA 1 cut(s) 188
BsaHI GRCGYC 2 cut(s) 5, 15
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 2 cut(s) 73, 141
BseAI TCCGGA 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 68
BsiSI CCGG 2 cut(s) 74, 142
BsiWI CGTACG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 2
BsmAI GTCTC 1 cut(s) 156
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 2
Bsp119I TTCGAA 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 68
Bsp13I TCCGGA 1 cut(s) 73
Bsp143I GATC 1 cut(s) 257
BspACI CCGC 3 cut(s) 23, 81, 83
BspCNI CTCAG 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 73
BspFNI CGCG 1 cut(s) 83
BspPI GGATC 1 cut(s) 265
BspT104I TTCGAA 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 1 cut(s) 257
BssNI GRCGYC 2 cut(s) 5, 15
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 2 cut(s) 5, 15
BstBI TTCGAA 1 cut(s) 188
BstDEI CTNAG 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 81
BstFNI CGCG 1 cut(s) 83
BstKTI GATC 1 cut(s) 260
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 1 cut(s) 257
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstUI CGCG 1 cut(s) 83
BtgI CCRYGG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 29
Cfr42I CCGCGG 1 cut(s) 84
CseI GACGC 2 cut(s) 13, 138
Csp6I GTAC 2 cut(s) 10, 194
CviAII CATG 1 cut(s) 119
CviJI RGCY 4 cut(s) 66, 175, 203, 248
CviKI_1 RGCY 4 cut(s) 66, 175, 203, 248
CviQI GTAC 2 cut(s) 10, 194
DdeI CTNAG 1 cut(s) 287
DpnI GATC 1 cut(s) 259
DpnII GATC 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 68
Eco53kI GAGCTC 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 1 cut(s) 122
FaiI YATR 3 cut(s) 120, 271, 310
FaqI GGGAC 1 cut(s) 2
FatI CATG 1 cut(s) 118
FriOI GRGCYC 1 cut(s) 68
HapII CCGG 2 cut(s) 74, 142
HgaI GACGC 2 cut(s) 13, 138
Hin1I GRCGYC 2 cut(s) 5, 15
Hin1II CATG 1 cut(s) 122
HinfI GANTC 4 cut(s) 77, 93, 126, 242
HpaII CCGG 2 cut(s) 74, 142
HphI GGTGA 1 cut(s) 150
Hpy188I TCNGA 2 cut(s) 92, 288
Hpy188III TCNNGA 2 cut(s) 74, 236
Hpy99I CGWCG 2 cut(s) 17, 242
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 2 cut(s) 5, 15
Hsp92II CATG 1 cut(s) 122
Kpn2I TCCGGA 1 cut(s) 73
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 1 cut(s) 257
LmnI GCTCC 1 cut(s) 63
LpnPI CCDG 5 cut(s) 87, 155, 185, 240, 285
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 47
MalI GATC 1 cut(s) 259
MboI GATC 1 cut(s) 257
MboII GAAGA 1 cut(s) 230
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 2 cut(s) 184, 275
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 2 cut(s) 38, 119
MroI TCCGGA 1 cut(s) 73
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 2 cut(s) 74, 142
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 1 cut(s) 257
NlaIII CATG 1 cut(s) 122
NspV TTCGAA 1 cut(s) 188
PfeI GAWTC 3 cut(s) 93, 126, 242
Pfl23II CGTACG 1 cut(s) 9
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
Psp124BI GAGCTC 1 cut(s) 68
PspLI CGTACG 1 cut(s) 9
RsaI GTAC 2 cut(s) 11, 195
RsaNI GTAC 2 cut(s) 10, 194
SacI GAGCTC 1 cut(s) 68
SacII CCGCGG 1 cut(s) 84
Sau3AI GATC 1 cut(s) 257
SchI GAGTC 1 cut(s) 86
SduI GDGCHC 1 cut(s) 68
SetI ASST 7 cut(s) 18, 49, 68, 177, 205, 250, 286
Sfr303I CCGCGG 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 188
SgrBI CCGCGG 1 cut(s) 84
Sse9I AATT 2 cut(s) 184, 275
SsiI CCGC 3 cut(s) 23, 81, 83
SstI GAGCTC 1 cut(s) 68
TaiI ACGT 1 cut(s) 18
TaqI TCGA 3 cut(s) 124, 188, 260
TasI AATT 2 cut(s) 184, 275
TatI WGTACW 1 cut(s) 193
TfiI GAWTC 3 cut(s) 93, 126, 242
TscAI CASTG 1 cut(s) 36
TspDTI ATGAA 2 cut(s) 107, 195
TspRI CASTG 1 cut(s) 36
XapI RAATTY 1 cut(s) 275
ZraI GACGTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.