RLG00000003932

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012688
Physical Location & Seq
Forward (+)
54420552 .. 54422845
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003932

Sequence Viewer

Length: 507 bp
ATGTCCTCACCACCTTCAGGCTCAACCTCCAGGATCCGATTGGTGCATGGAACTGCTGGGATTCCTCAGCGATCTCCACTCCCTGTGACTGGCGCCACTTCTTTTGAGACAATACTACTCGGGTCTCCGAGCTCCGCTTGCCTCGCCTTTAACTCGGCGGCCAACTCAGAGTGGTCTACGACTCCTCTTGCGGCGGCGGATCGTGATATGCTCCCTTTCTCTTGGGTCTTTACAACCTGGGATTCAAGGCTGTGTTCGATTTGGGCAAGTGGTCGGCGACCTTTCGCTGGGAAATCGGAGTTGTTGGGTCGGATCCGGATATGGGATCCAAGGATTATGAAGACTTCTTGGAATTTTGGCCGTGAGGTTGGGTGGCGGAGGCACCTAGCTGCACGCCTCATTTATGAGGATTCCTGTCCGACTTTGCATCAGCCTTTCCAGCGGCAATCCTGGTTGCAATGGATACTGCGGTCGGGATCTTTGGAGCCAGAAGGTTGGAGCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.78

Weight (kDa)

10.23

Isoelectric Point (pI)

68.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 92, 381
AccI GTMKAC 1 cut(s) 176
AccIII TCCGGA 1 cut(s) 315
AciI CCGC 8 cut(s) 135, 158, 191, 194, 197, 376, 442, 469
AclWI GGATC 8 cut(s) 28, 41, 207, 307, 320, 320, 333, 484
AcoI YGGCCR 2 cut(s) 159, 358
AcsI RAATTY 1 cut(s) 352
AcyI GRCGYC 1 cut(s) 93
AfeI AGCGCT 1 cut(s) 502
AfiI CCNNNNNNNGG 4 cut(s) 17, 89, 287, 322
AgsI TTSAA 1 cut(s) 246
AjnI CCWGG 3 cut(s) 29, 236, 449
AluBI AGCT 2 cut(s) 132, 389
AluI AGCT 2 cut(s) 132, 389
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 2 cut(s) 101, 129
AlwI GGATC 8 cut(s) 28, 41, 207, 307, 320, 320, 333, 484
Ama87I CYCGRG 1 cut(s) 119
Aor13HI TCCGGA 1 cut(s) 315
Aor51HI AGCGCT 1 cut(s) 502
AoxI GGCC 2 cut(s) 159, 358
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 1 cut(s) 352
AspLEI GCGC 2 cut(s) 95, 503
AvaI CYCGRG 1 cut(s) 119
BaeI ACNNNNGTAYC 2 cut(s) 455, 488
BamHI GGATCC 3 cut(s) 33, 312, 325
BanI GGYRCC 2 cut(s) 92, 381
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 376
BceAI ACGGC 1 cut(s) 345
BciT130I CCWGG 3 cut(s) 31, 238, 451
BciVI GTATCC 1 cut(s) 456
BcoDI GTCTC 2 cut(s) 101, 129
BfaI CTAG 1 cut(s) 386
BfoI RGCGCY 2 cut(s) 96, 504
BfuI GTATCC 1 cut(s) 456
BisI GCNGC 5 cut(s) 159, 192, 195, 390, 443
BlsI GCNGC 5 cut(s) 160, 193, 196, 391, 444
Bme1390I CCNGG 3 cut(s) 31, 238, 451
BmeT110I CYCGRG 1 cut(s) 119
BmiI GGNNCC 6 cut(s) 35, 94, 314, 327, 383, 486
BmrFI CCNGG 3 cut(s) 31, 238, 451
BmsI GCATC 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 347
BpmI CTGGAG 1 cut(s) 13
Bpu10I CCTNAGC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 237, 329
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 89, 287, 322
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 464
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 3 cut(s) 31, 238, 451
BseDI CCNNGG 2 cut(s) 237, 329
BseLI CCNNNNNNNGG 4 cut(s) 17, 89, 287, 322
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 2 cut(s) 80, 180
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 376
BseYI CCCAGC 2 cut(s) 56, 287
BsgI GTGCAG 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 473
BshFI GGCC 2 cut(s) 161, 360
BshNI GGYRCC 2 cut(s) 92, 381
BsiEI CGRYCG 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 1 cut(s) 316
BslI CCNNNNNNNGG 4 cut(s) 17, 89, 287, 322
BsmAI GTCTC 2 cut(s) 101, 129
BsnI GGCC 2 cut(s) 161, 360
Bso31I GGTCTC 1 cut(s) 129
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 134
Bsp13I TCCGGA 1 cut(s) 315
Bsp143I GATC 6 cut(s) 33, 71, 199, 312, 325, 476
BspACI CCGC 8 cut(s) 135, 158, 191, 194, 197, 376, 442, 469
BspANI GGCC 2 cut(s) 161, 360
BspCNI CTCAG 2 cut(s) 79, 179
BspEI TCCGGA 1 cut(s) 315
BspLI GGNNCC 6 cut(s) 35, 94, 314, 327, 383, 486
BspPI GGATC 8 cut(s) 28, 41, 207, 307, 320, 320, 333, 484
BspT107I GGYRCC 2 cut(s) 92, 381
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 464
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 237, 329
BssMI GATC 6 cut(s) 33, 71, 199, 312, 325, 476
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 329
Bst2UI CCWGG 3 cut(s) 31, 238, 451
BstACI GRCGYC 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 139, 394
BstDEI CTNAG 3 cut(s) 66, 166, 504
BstH2I RGCGCY 2 cut(s) 96, 504
BstHHI GCGC 2 cut(s) 95, 503
BstKTI GATC 6 cut(s) 36, 74, 202, 315, 328, 479
BstMAI GTCTC 2 cut(s) 101, 129
BstMBI GATC 6 cut(s) 33, 71, 199, 312, 325, 476
BstMCI CGRYCG 1 cut(s) 473
BstMWI GCNNNNNNNGC 3 cut(s) 138, 143, 439
BstNI CCWGG 3 cut(s) 31, 238, 451
BstSCI CCNGG 3 cut(s) 29, 236, 449
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 1 cut(s) 347
BstX2I RGATCY 4 cut(s) 33, 312, 325, 476
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 4 cut(s) 33, 312, 325, 476
BsuI GTATCC 1 cut(s) 456
BsuRI GGCC 2 cut(s) 161, 360
Cac8I GCNNGC 2 cut(s) 139, 394
CfoI GCGC 2 cut(s) 95, 503
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 1 cut(s) 47
CviJI RGCY 8 cut(s) 21, 132, 161, 250, 360, 389, 433, 487
CviKI_1 RGCY 8 cut(s) 21, 132, 161, 250, 360, 389, 433, 487
DdeI CTNAG 3 cut(s) 66, 166, 504
DinI GGCGCC 1 cut(s) 94
DpnI GATC 6 cut(s) 35, 73, 201, 314, 327, 478
DpnII GATC 6 cut(s) 33, 71, 199, 312, 325, 476
EaeI YGGCCR 2 cut(s) 159, 358
EciI GGCGGA 2 cut(s) 212, 391
Ecl136II GAGCTC 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 129
Eco47III AGCGCT 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 132
EcoRII CCWGG 3 cut(s) 29, 236, 449
EcoT14I CCWWGG 1 cut(s) 329
EcoT38I GRGCYC 1 cut(s) 134
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 1 cut(s) 50
FaiI YATR 5 cut(s) 48, 209, 322, 338, 405
FatI CATG 1 cut(s) 46
FblI GTMKAC 1 cut(s) 176
Fnu4HI GCNGC 5 cut(s) 159, 192, 195, 390, 443
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 5 cut(s) 159, 192, 195, 390, 443
FspBI CTAG 1 cut(s) 386
GlaI GCGC 2 cut(s) 94, 502
GluI GCNGC 5 cut(s) 159, 192, 195, 390, 443
GsaI CCCAGC 2 cut(s) 60, 291
GsuI CTGGAG 1 cut(s) 13
HaeII RGCGCY 2 cut(s) 96, 504
HaeIII GGCC 2 cut(s) 161, 360
HapII CCGG 1 cut(s) 316
HhaI GCGC 2 cut(s) 95, 503
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 1 cut(s) 50
Hin6I GCGC 2 cut(s) 93, 501
HinP1I GCGC 2 cut(s) 93, 501
HinfI GANTC 4 cut(s) 61, 181, 242, 410
HpaII CCGG 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 6 cut(s) 38, 129, 169, 298, 312, 420
Hpy188III TCNNGA 3 cut(s) 203, 316, 474
Hpy8I GTNNAC 1 cut(s) 177
HpyAV CCTTC 2 cut(s) 24, 485
HpyCH4V TGCA 4 cut(s) 46, 392, 427, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 143, 439
HpyF3I CTNAG 3 cut(s) 66, 166, 504
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 1 cut(s) 50
HspAI GCGC 2 cut(s) 93, 501
KasI GGCGCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 315
Kzo9I GATC 6 cut(s) 33, 71, 199, 312, 325, 476
LmnI GCTCC 4 cut(s) 137, 216, 484, 498
Lsp1109I GCAGC 1 cut(s) 376
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 85
MalI GATC 6 cut(s) 35, 73, 201, 314, 327, 478
MboI GATC 6 cut(s) 33, 71, 199, 312, 325, 476
MboII GAAGA 1 cut(s) 352
MflI RGATCY 4 cut(s) 33, 312, 325, 476
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 1 cut(s) 352
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 3 cut(s) 290, 443, 476
MnlI CCTC 9 cut(s) 16, 37, 75, 152, 195, 358, 372, 400, 407
MroI TCCGGA 1 cut(s) 315
MseI TTAA 1 cut(s) 150
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 1 cut(s) 316
MspR9I CCNGG 3 cut(s) 31, 238, 451
MvaI CCWGG 3 cut(s) 31, 238, 451
MwoI GCNNNNNNNGC 3 cut(s) 138, 143, 439
NarI GGCGCC 1 cut(s) 93
NdeII GATC 6 cut(s) 33, 71, 199, 312, 325, 476
NlaIII CATG 1 cut(s) 50
NlaIV GGNNCC 6 cut(s) 35, 94, 314, 327, 383, 486
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 61, 242, 410
PfoI TCCNGGA 1 cut(s) 29
PkrI GCNGC 5 cut(s) 160, 193, 196, 391, 444
PleI GAGTC 1 cut(s) 175
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 134
Psp6I CCWGG 3 cut(s) 29, 236, 449
PspFI CCCAGC 2 cut(s) 56, 287
PspGI CCWGG 3 cut(s) 29, 236, 449
PspN4I GGNNCC 6 cut(s) 35, 94, 314, 327, 383, 486
PsuI RGATCY 4 cut(s) 33, 312, 325, 476
SacI GAGCTC 1 cut(s) 134
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 5 cut(s) 159, 192, 195, 390, 443
Sau3AI GATC 6 cut(s) 33, 71, 199, 312, 325, 476
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 3 cut(s) 31, 238, 451
SduI GDGCHC 1 cut(s) 134
SetI ASST 9 cut(s) 16, 29, 134, 239, 283, 369, 387, 391, 496
SfaNI GCATC 1 cut(s) 436
SfoI GGCGCC 1 cut(s) 94
Sse9I AATT 1 cut(s) 352
SsiI CCGC 8 cut(s) 135, 158, 191, 194, 197, 376, 442, 469
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 134
StyD4I CCNGG 3 cut(s) 29, 236, 449
StyI CCWWGG 1 cut(s) 329
TaqI TCGA 1 cut(s) 257
TasI AATT 1 cut(s) 352
TauI GCSGC 4 cut(s) 161, 194, 197, 445
TfiI GAWTC 3 cut(s) 61, 242, 410
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 353
XapI RAATTY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmiI GTMKAC 1 cut(s) 176
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.