RLG00000020729

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012688
Physical Location & Seq
Reverse (-)
68027648 .. 68030168
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020729

Sequence Viewer

Length: 834 bp
ATGGGTGGTCGTTTTTACAACTTTCTCCATCTTCGATGGAGAACTATGTTGGCACATCCCTTGGTGTCGTATACCTCGAGTGCGGTGCAAACCGTCTCTTCTTTTCTTCCAATTTGCTTTTCAAAAGCGTGGTCTAAGACTCCTCTTGCGGCTGTAGATTGCGATGTTTTCCCTCTCTCTCAGGTCTGCACAACTTGGGATTCAAGGCTGAATTCGATCTGGGCAAGTGGTCGGCGACCTTCCGATGGGATATCGAAGGTTTTGGGTCGGATCCGGATTTGGGATCCAGGGACTATGTGGACTTCTTGGAATTTTGGCCGTGATTTCCTCTTGGAATTTCGGGGAGTAGGGTTGTGGGAGGGGCTCGAGATGGGTAGGTTTTTGACCCAGATTGAAGGGCAGTTATTGGAACGGCCGCGGGTGTCAAAATGGTTTTCTGGTTTCTGGTTCCCAGATCGGACTAGATGGAGTTCGCGACTAGAGATTTCATGTCAGAGACCGACGGCAGGGCAGCAGTCCGTGAGTGGTGGCGCTTGGAGTCATGTGGACGAAACCAGGAAGAGAGATTTCCGGCATTGCGGATCTGGAAGGGTGGTCGGACATCCGACTGCAGCCAGGACTAGTCACCGAGCTGCAGACCCGATGCTCTCAAAGACCACCACAACGTCTTTCCTGTTGTCGTTCCTCTATGCAGACCATCGGCGCTCTGACCATCGATGTTCGACCCAAATTCCCACGGTACCAGATCGGACCAGCTTCTCCAGATCGGACTCGGCTTTTCGTGGGCCTGTGCTGACCCACCAGATCGAATCTTCTGACGTCGACAACTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

278

Amino Acids

31.5

Weight (kDa)

9.84

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 822
Acc65I GGTACC 1 cut(s) 739
AccB1I GGYRCC 1 cut(s) 739
AccI GTMKAC 2 cut(s) 71, 822
AccII CGCG 2 cut(s) 418, 475
AccIII TCCGGA 1 cut(s) 273
AciI CCGC 5 cut(s) 83, 149, 416, 418, 579
AclWI GGATC 5 cut(s) 265, 278, 278, 291, 589
AcoI YGGCCR 2 cut(s) 316, 413
AcsI RAATTY 4 cut(s) 211, 310, 335, 729
AcyI GRCGYC 1 cut(s) 819
AfaI GTAC 1 cut(s) 741
AfiI CCNNNNNNNGG 3 cut(s) 245, 280, 506
AgsI TTSAA 3 cut(s) 123, 204, 395
AhlI ACTAGT 1 cut(s) 620
AjnI CCWGG 3 cut(s) 286, 554, 614
AjuI GAANNNNNNNTTGG 2 cut(s) 103, 135
AluBI AGCT 2 cut(s) 632, 756
AluI AGCT 2 cut(s) 632, 756
Alw26I GTCTC 2 cut(s) 100, 490
AlwI GGATC 5 cut(s) 265, 278, 278, 291, 589
Ama87I CYCGRG 2 cut(s) 76, 365
Aor13HI TCCGGA 1 cut(s) 273
AoxI GGCC 3 cut(s) 316, 413, 785
ApeKI GCWGC 3 cut(s) 511, 611, 632
ApoI RAATTY 4 cut(s) 211, 310, 335, 729
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
Asp718I GGTACC 1 cut(s) 739
AspLEI GCGC 2 cut(s) 533, 705
AspS9I GGNCC 2 cut(s) 750, 785
AsuHPI GGTGA 1 cut(s) 617
AvaI CYCGRG 2 cut(s) 76, 365
AvaII GGWCC 1 cut(s) 750
BamHI GGATCC 2 cut(s) 270, 283
BanI GGYRCC 1 cut(s) 739
BanII GRGCYC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 523, 619, 623
BccI CCATC 7 cut(s) 30, 36, 239, 364, 459, 705, 720
BceAI ACGGC 3 cut(s) 303, 428, 519
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 3 cut(s) 288, 556, 616
BcoDI GTCTC 2 cut(s) 100, 490
BcuI ACTAGT 1 cut(s) 620
BfaI CTAG 3 cut(s) 462, 479, 621
BfmI CTRYAG 3 cut(s) 153, 609, 633
BfoI RGCGCY 2 cut(s) 534, 706
BisI GCNGC 5 cut(s) 150, 416, 512, 612, 633
BlsI GCNGC 5 cut(s) 151, 417, 513, 613, 634
Bme1390I CCNGG 3 cut(s) 288, 556, 616
Bme18I GGWCC 1 cut(s) 750
BmeT110I CYCGRG 2 cut(s) 76, 365
BmgT120I GGNCC 2 cut(s) 750, 785
BmiI GGNNCC 4 cut(s) 272, 285, 449, 741
BmrFI CCNGG 3 cut(s) 288, 556, 616
BmsI GCATC 1 cut(s) 633
BpmI CTGGAG 1 cut(s) 745
Bsa29I ATCGAT 1 cut(s) 715
BsaHI GRCGYC 1 cut(s) 819
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 4 cut(s) 60, 287, 416, 735
BsaWI WCCGGW 1 cut(s) 273
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 280, 506
Bse3DI GCAATG 1 cut(s) 574
BseAI TCCGGA 1 cut(s) 273
BseBI CCWGG 3 cut(s) 288, 556, 616
BseCI ATCGAT 1 cut(s) 715
BseDI CCNNGG 4 cut(s) 60, 287, 416, 735
BseGI GGATG 2 cut(s) 55, 601
BseLI CCNNNNNNNGG 3 cut(s) 245, 280, 506
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 132
BseX3I CGGCCG 1 cut(s) 413
BseXI GCAGC 3 cut(s) 523, 619, 623
BsgI GTGCAG 1 cut(s) 172
Bsh1236I CGCG 2 cut(s) 418, 475
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 3 cut(s) 318, 415, 787
BshNI GGYRCC 1 cut(s) 739
BshVI ATCGAT 1 cut(s) 715
BsiEI CGRYCG 1 cut(s) 416
BsiHKCI CYCGRG 2 cut(s) 76, 365
BsiSI CCGG 2 cut(s) 274, 571
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 3 cut(s) 245, 280, 506
BsmAI GTCTC 2 cut(s) 100, 490
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 3 cut(s) 318, 415, 787
Bso31I GGTCTC 1 cut(s) 490
BsoBI CYCGRG 2 cut(s) 76, 365
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 273
Bsp143I GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
Bsp68I TCGCGA 1 cut(s) 475
BspACI CCGC 5 cut(s) 83, 149, 416, 418, 579
BspANI GGCC 3 cut(s) 318, 415, 787
BspCNI CTCAG 1 cut(s) 193
BspDI ATCGAT 1 cut(s) 715
BspEI TCCGGA 1 cut(s) 273
BspFNI CGCG 2 cut(s) 418, 475
BspLI GGNNCC 4 cut(s) 272, 285, 449, 741
BspMAI CTGCAG 2 cut(s) 613, 637
BspPI GGATC 5 cut(s) 265, 278, 278, 291, 589
BspT107I GGYRCC 1 cut(s) 739
BspTNI GGTCTC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 574
BssECI CCNNGG 4 cut(s) 60, 287, 416, 735
BssMI GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
BssNAI GTATAC 1 cut(s) 72
BssNI GRCGYC 1 cut(s) 819
BssT1I CCWWGG 1 cut(s) 60
Bst1107I GTATAC 1 cut(s) 72
Bst2UI CCWGG 3 cut(s) 288, 556, 616
Bst4CI ACNGT 2 cut(s) 94, 739
Bst6I CTCTTC 2 cut(s) 103, 554
BstACI GRCGYC 1 cut(s) 819
BstDEI CTNAG 2 cut(s) 135, 180
BstDSI CCRYGG 2 cut(s) 416, 735
BstF5I GGATG 2 cut(s) 55, 601
BstFNI CGCG 2 cut(s) 418, 475
BstH2I RGCGCY 2 cut(s) 534, 706
BstHHI GCGC 2 cut(s) 533, 705
BstKTI GATC 8 cut(s) 219, 273, 286, 457, 584, 748, 767, 807
BstMAI GTCTC 2 cut(s) 100, 490
BstMBI GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
BstMCI CGRYCG 1 cut(s) 416
BstNI CCWGG 3 cut(s) 288, 556, 616
BstSCI CCNGG 3 cut(s) 286, 554, 614
BstSFI CTRYAG 3 cut(s) 153, 609, 633
BstUI CGCG 2 cut(s) 418, 475
BstV1I GCAGC 3 cut(s) 523, 619, 623
BstX2I RGATCY 3 cut(s) 270, 283, 581
BstYI RGATCY 3 cut(s) 270, 283, 581
BstZ17I GTATAC 1 cut(s) 72
BstZI CGGCCG 1 cut(s) 413
Bsu15I ATCGAT 1 cut(s) 715
BsuRI GGCC 3 cut(s) 318, 415, 787
BsuTUI ATCGAT 1 cut(s) 715
BtgI CCRYGG 2 cut(s) 416, 735
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 2 cut(s) 55, 601
BtuMI TCGCGA 1 cut(s) 475
CfoI GCGC 2 cut(s) 533, 705
Cfr13I GGNCC 2 cut(s) 750, 785
Cfr42I CCGCGG 1 cut(s) 419
ClaI ATCGAT 1 cut(s) 715
Csp6I GTAC 1 cut(s) 740
CviAII CATG 2 cut(s) 489, 542
CviQI GTAC 1 cut(s) 740
DdeI CTNAG 2 cut(s) 135, 180
DpnI GATC 8 cut(s) 218, 272, 285, 456, 583, 747, 766, 806
DpnII GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
EaeI YGGCCR 2 cut(s) 316, 413
EagI CGGCCG 1 cut(s) 413
Eam1104I CTCTTC 2 cut(s) 103, 554
EarI CTCTTC 2 cut(s) 103, 554
EclXI CGGCCG 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 490
Eco32I GATATC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 750
Eco52I CGGCCG 1 cut(s) 413
Eco88I CYCGRG 2 cut(s) 76, 365
EcoRI GAATTC 1 cut(s) 211
EcoRII CCWGG 3 cut(s) 286, 554, 614
EcoRV GATATC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 60
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 2 cut(s) 492, 545
FaiI YATR 6 cut(s) 47, 72, 296, 490, 543, 690
FaqI GGGAC 1 cut(s) 304
FatI CATG 2 cut(s) 488, 541
FauI CCCGC 1 cut(s) 411
FblI GTMKAC 2 cut(s) 71, 822
Fnu4HI GCNGC 5 cut(s) 150, 416, 512, 612, 633
FokI GGATG 2 cut(s) 42, 588
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 5 cut(s) 150, 416, 512, 612, 633
FspBI CTAG 3 cut(s) 462, 479, 621
GlaI GCGC 2 cut(s) 532, 704
GluI GCNGC 5 cut(s) 150, 416, 512, 612, 633
GsuI CTGGAG 1 cut(s) 745
HaeII RGCGCY 2 cut(s) 534, 706
HaeIII GGCC 3 cut(s) 318, 415, 787
HapII CCGG 2 cut(s) 274, 571
HhaI GCGC 2 cut(s) 533, 705
Hin1I GRCGYC 1 cut(s) 819
Hin1II CATG 2 cut(s) 492, 545
Hin6I GCGC 2 cut(s) 531, 703
HinP1I GCGC 2 cut(s) 531, 703
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 5 cut(s) 139, 200, 538, 770, 809
HpaII CCGG 2 cut(s) 274, 571
HphI GGTGA 1 cut(s) 617
Hpy166II GTNNAC 4 cut(s) 72, 300, 547, 823
Hpy188III TCNNGA 5 cut(s) 274, 367, 474, 585, 762
Hpy8I GTNNAC 4 cut(s) 72, 300, 547, 823
Hpy99I CGWCG 2 cut(s) 505, 824
HpyAV CCTTC 4 cut(s) 249, 250, 389, 582
HpyCH4III ACNGT 2 cut(s) 94, 739
HpyCH4IV ACGT 2 cut(s) 665, 819
HpyCH4V TGCA 5 cut(s) 88, 189, 611, 635, 692
HpyF3I CTNAG 2 cut(s) 135, 180
HpySE526I ACGT 2 cut(s) 665, 819
Hsp92I GRCGYC 1 cut(s) 819
Hsp92II CATG 2 cut(s) 492, 545
HspAI GCGC 2 cut(s) 531, 703
Kpn2I TCCGGA 1 cut(s) 273
KpnI GGTACC 1 cut(s) 743
KspI CCGCGG 1 cut(s) 419
Kzo9I GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
Lsp1109I GCAGC 3 cut(s) 523, 619, 623
LweI GCATC 1 cut(s) 633
MaeI CTAG 3 cut(s) 462, 479, 621
MaeII ACGT 2 cut(s) 665, 819
MaeIII GTNAC 1 cut(s) 623
MalI GATC 8 cut(s) 218, 272, 285, 456, 583, 747, 766, 806
MboI GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
MboII GAAGA 5 cut(s) 23, 90, 98, 571, 804
MflI RGATCY 3 cut(s) 270, 283, 581
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 5 cut(s) 111, 211, 310, 335, 729
MlyI GAGTC 3 cut(s) 133, 547, 764
MmeI TCCRAC 3 cut(s) 248, 577, 629
MnlI CCTC 6 cut(s) 85, 153, 183, 338, 352, 695
MroI TCCGGA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 2 cut(s) 274, 571
MspR9I CCNGG 3 cut(s) 288, 556, 616
MvaI CCWGG 3 cut(s) 288, 556, 616
MvnI CGCG 2 cut(s) 418, 475
NdeII GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
NlaIII CATG 2 cut(s) 492, 545
NlaIV GGNNCC 4 cut(s) 272, 285, 449, 741
NmeAIII GCCGAG 1 cut(s) 752
NmuCI GTSAC 1 cut(s) 623
NruI TCGCGA 1 cut(s) 475
PaeR7I CTCGAG 2 cut(s) 76, 365
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PfeI GAWTC 2 cut(s) 200, 809
PkrI GCNGC 5 cut(s) 151, 417, 513, 613, 634
PleI GAGTC 3 cut(s) 133, 546, 764
PpsI GAGTC 3 cut(s) 133, 546, 764
Psp6I CCWGG 3 cut(s) 286, 554, 614
PspGI CCWGG 3 cut(s) 286, 554, 614
PspN4I GGNNCC 4 cut(s) 272, 285, 449, 741
PspPI GGNCC 2 cut(s) 750, 785
PspXI VCTCGAGB 1 cut(s) 76
PstI CTGCAG 2 cut(s) 613, 637
PsuI RGATCY 3 cut(s) 270, 283, 581
RruI TCGCGA 1 cut(s) 475
RsaI GTAC 1 cut(s) 741
RsaNI GTAC 1 cut(s) 740
SacII CCGCGG 1 cut(s) 419
SalI GTCGAC 1 cut(s) 821
SatI GCNGC 5 cut(s) 150, 416, 512, 612, 633
Sau3AI GATC 8 cut(s) 216, 270, 283, 454, 581, 745, 764, 804
Sau96I GGNCC 2 cut(s) 750, 785
SchI GAGTC 3 cut(s) 133, 547, 764
ScrFI CCNGG 3 cut(s) 288, 556, 616
SduI GDGCHC 1 cut(s) 366
SetI ASST 9 cut(s) 77, 186, 241, 261, 380, 634, 668, 758, 822
SfaNI GCATC 1 cut(s) 633
SfcI CTRYAG 3 cut(s) 153, 609, 633
Sfr274I CTCGAG 2 cut(s) 76, 365
Sfr303I CCGCGG 1 cut(s) 419
SgrBI CCGCGG 1 cut(s) 419
SinI GGWCC 1 cut(s) 750
SlaI CTCGAG 2 cut(s) 76, 365
SmlI CTYRAG 2 cut(s) 76, 365
SmoI CTYRAG 2 cut(s) 76, 365
SpeI ACTAGT 1 cut(s) 620
Sse9I AATT 5 cut(s) 111, 211, 310, 335, 729
SsiI CCGC 5 cut(s) 83, 149, 416, 418, 579
SspMI CTAG 3 cut(s) 462, 479, 621
StyD4I CCNGG 3 cut(s) 286, 554, 614
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 94, 739
TaiI ACGT 2 cut(s) 668, 822
TaqI TCGA 9 cut(s) 34, 77, 215, 254, 366, 715, 722, 807, 822
TaqII GACCGA 1 cut(s) 514
TasI AATT 5 cut(s) 111, 211, 310, 335, 729
TauI GCSGC 2 cut(s) 152, 418
TfiI GAWTC 2 cut(s) 200, 809
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 3 cut(s) 511, 611, 632
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 1 cut(s) 477
TspGWI ACGGA 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 750
XapI RAATTY 4 cut(s) 211, 310, 335, 729
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XhoI CTCGAG 2 cut(s) 76, 365
XmiI GTMKAC 2 cut(s) 71, 822
XspI CTAG 3 cut(s) 462, 479, 621
ZraI GACGTC 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.