RLG00000011916

Phytochrome A-associated F-box

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012400
Physical Location & Seq
Forward (+)
13763017 .. 13763964
948 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011916

Sequence Viewer

Length: 948 bp
ATGAATTCCGACTCCGTGTTCTCGACGCTTCCGGACGACGTCGTTCTGAACTTGTTCTCCAAGCTGGAGGACGACCCTCGCAACTGGGCCCGCCTCGCCTGCGCCTGCACAAAATTCGCCTCCCTGATTCGCAACGTCTGCTGGAAGACCAAGTGCCGCAACGTCCTCCCCTCCGTCGTCTCCGATCTCGTCTCCGCCGCCTCCTCCTCCGAGCCCCCCGGCGGCTGGTCCTCCCTCCACAAGCTGGCCGTCTGCTGCCCCGGCCTCCTCCACTCCGGCGTCCTCTTCGAGAATTCCGATTTCGGCCTCGAGCGCGAGCTCGGCCCCGACGAAAGTTACCGCCCCCAATCGCAATGGGAGCCCTCCTCCAGCCAAATTCACCAACCACCGTCGCCGCCGCCGGCTTCCGATTGCGCTTGGTCTCTGTACGACGATCTGTATTTCGATACGGTGTACGACGACTCCGAATCGGATGCTGTTGCTGCCCCCGAGAACGGCGTCGTTTCAGTGGGCGGTGAGTTTCCGATTCAAAAAAGGCGAAAGATCTGCAGGTCGATGAGGTCGCATTTGGCTTCCGGGGTCTGGAATCTGAGCCGCGAGCAAGGCAACAAGCTTCTCCACAGCCGGTTTCGCGGCGACTGCCTCTACATCTGTGACTGGCCTGGGTGTGTTCACATTGAAGAGAAGCGGAATTACATGCTTTTCAGAGGGGTTTTCAAGGACTTCAAAGGCTCTCAAGTGTGGAGGAACATCAAGGATGCCAAGAGGACCAAAGTTGACCTCAATTGCGCCTACTGCACCTGCAAGCAGACTTGGGATTTGCATTCCGCCTTCTGTTTGAGACGCGGTTTCGGCTACCATGACGATGGCGAACCCGTTGTCAGGGCTTATGTGTGCGACAATGGACATGTCTCCGGGGCCTGGACCGACTTGCCATTGTATGCCTAA

Protein Analysis

316

Amino Acids

35.39

Weight (kDa)

6.14

Isoelectric Point (pI)

59.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 7 - 53 1.8e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 809
AatII GACGTC 1 cut(s) 42
Acc36I ACCTGC 2 cut(s) 540, 809
AccB7I CCANNNNNTGG 1 cut(s) 244
AccII CGCG 4 cut(s) 315, 597, 633, 846
AccIII TCCGGA 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 4 cut(s) 4, 113, 292, 375
AcyI GRCGYC 3 cut(s) 39, 279, 498
AfaI GTAC 2 cut(s) 428, 455
AfiI CCNNNNNNNGG 7 cut(s) 221, 225, 244, 494, 582, 882, 921
AflIII ACRYGT 1 cut(s) 907
AgsI TTSAA 4 cut(s) 530, 680, 718, 727
AjnI CCWGG 2 cut(s) 661, 920
AloI GAACNNNNNNTCC 2 cut(s) 41, 73
AluBI AGCT 4 cut(s) 64, 244, 319, 613
AluI AGCT 4 cut(s) 64, 244, 319, 613
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 5 cut(s) 184, 196, 426, 835, 916
Ama87I CYCGRG 2 cut(s) 308, 488
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 7 cut(s) 87, 246, 262, 304, 322, 659, 918
ApaI GGGCCC 1 cut(s) 91
ApeKI GCWGC 2 cut(s) 255, 482
ApoI RAATTY 4 cut(s) 4, 113, 292, 375
ArsI GACNNNNNNTTYG 4 cut(s) 802, 834, 864, 896
Asp700I GAANNNNTTC 1 cut(s) 53
AspLEI GCGC 4 cut(s) 104, 315, 416, 791
AspS9I GGNCC 7 cut(s) 87, 88, 228, 323, 768, 918, 924
AsuC2I CCSGG 4 cut(s) 219, 261, 577, 916
AsuHPI GGTGA 2 cut(s) 371, 527
AvaI CYCGRG 2 cut(s) 308, 488
AvaII GGWCC 3 cut(s) 228, 768, 924
BaeGI GKGCMC 1 cut(s) 91
BanII GRGCYC 4 cut(s) 91, 216, 321, 363
BarI GAAGNNNNNNTAC 2 cut(s) 677, 709
BbsI GAAGAC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 242, 469
BccI CCATC 1 cut(s) 860
BceAI ACGGC 2 cut(s) 233, 511
BcgI CGANNNNNNTGC 6 cut(s) 97, 131, 455, 489, 528, 562
BciT130I CCWGG 2 cut(s) 663, 922
BcnI CCSGG 4 cut(s) 219, 261, 577, 916
BcoDI GTCTC 5 cut(s) 184, 196, 426, 835, 916
BfmI CTRYAG 1 cut(s) 547
BfuAI ACCTGC 2 cut(s) 540, 809
BglII AGATCT 1 cut(s) 543
BisI GCNGC 9 cut(s) 157, 198, 223, 256, 395, 398, 483, 595, 634
BlsI GCNGC 9 cut(s) 158, 199, 224, 257, 396, 399, 484, 596, 635
Bme1390I CCNGG 6 cut(s) 219, 261, 577, 663, 916, 922
Bme18I GGWCC 3 cut(s) 228, 768, 924
BmeT110I CYCGRG 2 cut(s) 308, 488
BmgT120I GGNCC 7 cut(s) 87, 88, 228, 323, 768, 918, 924
BmiI GGNNCC 4 cut(s) 89, 325, 360, 919
BmrFI CCNGG 6 cut(s) 219, 261, 577, 663, 916, 922
BmrI ACTGGG 1 cut(s) 94
BmsI GCATC 2 cut(s) 463, 748
BmuI ACTGGG 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 152
BplI GAGNNNNNCTC 2 cut(s) 350, 382
BpmI CTGGAG 2 cut(s) 86, 352
BpuEI CTTGAG 1 cut(s) 720
BpuMI CCSGG 4 cut(s) 219, 261, 577, 916
BsaHI GRCGYC 3 cut(s) 39, 279, 498
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 5 cut(s) 217, 259, 576, 662, 915
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 446, 476
Bsc4I CCNNNNNNNGG 7 cut(s) 221, 225, 244, 494, 582, 882, 921
Bse118I RCCGGY 2 cut(s) 400, 624
Bse1I ACTGG 2 cut(s) 89, 662
Bse3DI GCAATG 1 cut(s) 359
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 2 cut(s) 663, 922
BseDI CCNNGG 5 cut(s) 217, 259, 576, 662, 915
BseGI GGATG 2 cut(s) 478, 763
BseLI CCNNNNNNNGG 7 cut(s) 221, 225, 244, 494, 582, 882, 921
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 2 cut(s) 89, 662
BseRI GAGGAG 4 cut(s) 193, 196, 257, 355
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 2 cut(s) 242, 469
BsgI GTGCAG 2 cut(s) 91, 781
Bsh1236I CGCG 4 cut(s) 315, 597, 633, 846
BshFI GGCC 7 cut(s) 89, 248, 264, 306, 324, 661, 920
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 2 cut(s) 308, 488
BsiSI CCGG 8 cut(s) 32, 219, 261, 276, 401, 576, 625, 915
BslI CCNNNNNNNGG 7 cut(s) 221, 225, 244, 494, 582, 882, 921
BsmAI GTCTC 5 cut(s) 184, 196, 426, 835, 916
BsmBI CGTCTC 3 cut(s) 184, 196, 835
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 7 cut(s) 89, 248, 264, 306, 324, 661, 920
Bso31I GGTCTC 1 cut(s) 426
BsoBI CYCGRG 2 cut(s) 308, 488
Bsp120I GGGCCC 1 cut(s) 87
Bsp1286I GDGCHC 4 cut(s) 91, 216, 321, 363
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 3 cut(s) 184, 433, 543
BspANI GGCC 7 cut(s) 89, 248, 264, 306, 324, 661, 920
BspCNI CTCAG 1 cut(s) 582
BspEI TCCGGA 1 cut(s) 31
BspFNI CGCG 4 cut(s) 315, 597, 633, 846
BspLI GGNNCC 4 cut(s) 89, 325, 360, 919
BspMAI CTGCAG 1 cut(s) 551
BspMI ACCTGC 2 cut(s) 540, 809
BspTNI GGTCTC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 359
BsrFI RCCGGY 2 cut(s) 400, 624
BsrI ACTGG 2 cut(s) 89, 662
BssAI RCCGGY 2 cut(s) 400, 624
BssECI CCNNGG 5 cut(s) 217, 259, 576, 662, 915
BssMI GATC 3 cut(s) 184, 433, 543
BssNI GRCGYC 3 cut(s) 39, 279, 498
Bst2UI CCWGG 2 cut(s) 663, 922
Bst4CI ACNGT 2 cut(s) 390, 451
Bst6I CTCTTC 2 cut(s) 290, 675
BstACI GRCGYC 3 cut(s) 39, 279, 498
BstAPI GCANNNNNTGC 1 cut(s) 138
BstC8I GCNNGC 8 cut(s) 91, 100, 106, 246, 317, 402, 599, 806
BstDEI CTNAG 1 cut(s) 590
BstF5I GGATG 2 cut(s) 478, 763
BstFNI CGCG 4 cut(s) 315, 597, 633, 846
BstHHI GCGC 4 cut(s) 104, 315, 416, 791
BstKTI GATC 3 cut(s) 187, 436, 546
BstMAI GTCTC 5 cut(s) 184, 196, 426, 835, 916
BstMBI GATC 3 cut(s) 184, 433, 543
BstNI CCWGG 2 cut(s) 663, 922
BstNSI RCATGY 2 cut(s) 700, 911
BstSCI CCNGG 6 cut(s) 217, 259, 575, 661, 914, 920
BstSFI CTRYAG 1 cut(s) 547
BstSLI GKGCMC 1 cut(s) 91
BstUI CGCG 4 cut(s) 315, 597, 633, 846
BstV1I GCAGC 2 cut(s) 242, 469
BstV2I GAAGAC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 543
BstXI CCANNNNNNTGG 1 cut(s) 866
BstYI RGATCY 1 cut(s) 543
BsuRI GGCC 7 cut(s) 89, 248, 264, 306, 324, 661, 920
BtsCI GGATG 2 cut(s) 478, 763
BtsIMutI CAGTG 1 cut(s) 513
BveI ACCTGC 2 cut(s) 540, 809
Cac8I GCNNGC 8 cut(s) 91, 100, 106, 246, 317, 402, 599, 806
CfoI GCGC 4 cut(s) 104, 315, 416, 791
Cfr10I RCCGGY 2 cut(s) 400, 624
Cfr13I GGNCC 7 cut(s) 87, 88, 228, 323, 768, 918, 924
CseI GACGC 4 cut(s) 34, 268, 487, 852
Csp6I GTAC 2 cut(s) 427, 454
CviAII CATG 3 cut(s) 697, 860, 908
CviQI GTAC 2 cut(s) 427, 454
DdeI CTNAG 1 cut(s) 590
DpnI GATC 3 cut(s) 186, 435, 545
DpnII GATC 3 cut(s) 184, 433, 543
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 290, 675
EarI CTCTTC 2 cut(s) 290, 675
EciI GGCGGA 2 cut(s) 184, 817
Ecl136II GAGCTC 1 cut(s) 319
Eco24I GRGCYC 4 cut(s) 91, 216, 321, 363
Eco31I GGTCTC 1 cut(s) 426
Eco47I GGWCC 3 cut(s) 228, 768, 924
Eco53kI GAGCTC 1 cut(s) 319
Eco88I CYCGRG 2 cut(s) 308, 488
EcoICRI GAGCTC 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 918
EcoRI GAATTC 2 cut(s) 4, 292
EcoRII CCWGG 2 cut(s) 661, 920
EcoT38I GRGCYC 4 cut(s) 91, 216, 321, 363
Esp3I CGTCTC 3 cut(s) 184, 196, 835
FaeI CATG 3 cut(s) 700, 863, 911
FaiI YATR 5 cut(s) 698, 861, 891, 909, 942
FatI CATG 3 cut(s) 696, 859, 907
FauI CCCGC 1 cut(s) 98
Fnu4HI GCNGC 9 cut(s) 157, 198, 223, 256, 395, 398, 483, 595, 634
FokI GGATG 2 cut(s) 485, 770
FriOI GRGCYC 4 cut(s) 91, 216, 321, 363
Fsp4HI GCNGC 9 cut(s) 157, 198, 223, 256, 395, 398, 483, 595, 634
GlaI GCGC 4 cut(s) 103, 314, 415, 790
GluI GCNGC 9 cut(s) 157, 198, 223, 256, 395, 398, 483, 595, 634
GsuI CTGGAG 2 cut(s) 86, 352
HaeIII GGCC 7 cut(s) 89, 248, 264, 306, 324, 661, 920
HapII CCGG 8 cut(s) 32, 219, 261, 276, 401, 576, 625, 915
HgaI GACGC 4 cut(s) 34, 268, 487, 852
HhaI GCGC 4 cut(s) 104, 315, 416, 791
Hin1I GRCGYC 3 cut(s) 39, 279, 498
Hin1II CATG 3 cut(s) 700, 863, 911
Hin6I GCGC 4 cut(s) 102, 313, 414, 789
HinP1I GCGC 4 cut(s) 102, 313, 414, 789
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 6 cut(s) 11, 127, 461, 467, 526, 586
HpaII CCGG 8 cut(s) 32, 219, 261, 276, 401, 576, 625, 915
HphI GGTGA 2 cut(s) 371, 527
Hpy166II GTNNAC 3 cut(s) 454, 673, 778
Hpy188III TCNNGA 4 cut(s) 22, 32, 289, 583
Hpy8I GTNNAC 3 cut(s) 454, 673, 778
Hpy99I CGWCG 9 cut(s) 28, 41, 44, 179, 332, 394, 434, 461, 503
HpyAV CCTTC 1 cut(s) 841
HpyCH4III ACNGT 2 cut(s) 390, 451
HpyCH4IV ACGT 3 cut(s) 39, 135, 162
HpyCH4V TGCA 5 cut(s) 108, 549, 798, 804, 823
HpyF3I CTNAG 1 cut(s) 590
HpySE526I ACGT 3 cut(s) 39, 135, 162
Hsp92I GRCGYC 3 cut(s) 39, 279, 498
Hsp92II CATG 3 cut(s) 700, 863, 911
HspAI GCGC 4 cut(s) 102, 313, 414, 789
Kpn2I TCCGGA 1 cut(s) 31
KroI GCCGGC 1 cut(s) 400
KroNI GCCGGC 1 cut(s) 402
Kzo9I GATC 3 cut(s) 184, 433, 543
LmnI GCTCC 1 cut(s) 358
Lsp1109I GCAGC 2 cut(s) 242, 469
LweI GCATC 2 cut(s) 463, 748
MaeII ACGT 3 cut(s) 39, 135, 162
MaeIII GTNAC 2 cut(s) 335, 653
MalI GATC 3 cut(s) 186, 435, 545
MboI GATC 3 cut(s) 184, 433, 543
MboII GAAGA 3 cut(s) 157, 277, 692
MfeI CAATTG 1 cut(s) 784
MflI RGATCY 1 cut(s) 543
MhlI GDGCHC 4 cut(s) 91, 216, 321, 363
MluCI AATT 6 cut(s) 4, 113, 292, 375, 691, 784
MlyI GAGTC 2 cut(s) 5, 455
MmeI TCCRAC 1 cut(s) 33
MroI TCCGGA 1 cut(s) 31
MroNI GCCGGC 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 864
MspI CCGG 8 cut(s) 32, 219, 261, 276, 401, 576, 625, 915
MspR9I CCNGG 6 cut(s) 219, 261, 577, 663, 916, 922
MunI CAATTG 1 cut(s) 784
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 2 cut(s) 663, 922
MvnI CGCG 4 cut(s) 315, 597, 633, 846
NaeI GCCGGC 1 cut(s) 402
NciI CCSGG 4 cut(s) 219, 261, 577, 916
NdeII GATC 3 cut(s) 184, 433, 543
NgoMIV GCCGGC 1 cut(s) 400
NlaIII CATG 3 cut(s) 700, 863, 911
NlaIV GGNNCC 4 cut(s) 89, 325, 360, 919
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 1 cut(s) 653
NspI RCATGY 2 cut(s) 700, 911
PaeR7I CTCGAG 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 809
PciI ACATGT 1 cut(s) 907
PcsI WCGNNNNNNNCGW 3 cut(s) 294, 327, 462
PctI GAATGC 1 cut(s) 823
PdiI GCCGGC 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 4 cut(s) 127, 467, 526, 586
PflFI GACNNNGTC 1 cut(s) 38
PflMI CCANNNNNTGG 1 cut(s) 244
PkrI GCNGC 9 cut(s) 158, 199, 224, 257, 396, 399, 484, 596, 635
PleI GAGTC 2 cut(s) 5, 455
PpsI GAGTC 2 cut(s) 5, 455
PscI ACATGT 1 cut(s) 907
Psp124BI GAGCTC 1 cut(s) 321
Psp6I CCWGG 2 cut(s) 661, 920
PspGI CCWGG 2 cut(s) 661, 920
PspN4I GGNNCC 4 cut(s) 89, 325, 360, 919
PspOMI GGGCCC 1 cut(s) 87
PspPI GGNCC 7 cut(s) 87, 88, 228, 323, 768, 918, 924
PspXI VCTCGAGB 1 cut(s) 308
PstI CTGCAG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 543
PsyI GACNNNGTC 1 cut(s) 38
RsaI GTAC 2 cut(s) 428, 455
RsaNI GTAC 2 cut(s) 427, 454
RseI CAYNNNNRTG 1 cut(s) 864
SacI GAGCTC 1 cut(s) 321
SatI GCNGC 9 cut(s) 157, 198, 223, 256, 395, 398, 483, 595, 634
Sau3AI GATC 3 cut(s) 184, 433, 543
Sau96I GGNCC 7 cut(s) 87, 88, 228, 323, 768, 918, 924
SchI GAGTC 2 cut(s) 5, 455
ScrFI CCNGG 6 cut(s) 219, 261, 577, 663, 916, 922
SduI GDGCHC 4 cut(s) 91, 216, 321, 363
SfaNI GCATC 2 cut(s) 463, 748
SfcI CTRYAG 1 cut(s) 547
Sfr274I CTCGAG 1 cut(s) 308
SinI GGWCC 3 cut(s) 228, 768, 924
SlaI CTCGAG 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 2 cut(s) 308, 735
SmoI CTYRAG 2 cut(s) 308, 735
Sse9I AATT 6 cut(s) 4, 113, 292, 375, 691, 784
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 6 cut(s) 217, 259, 575, 661, 914, 920
TaaI ACNGT 2 cut(s) 390, 451
TaiI ACGT 3 cut(s) 42, 138, 165
TaqI TCGA 5 cut(s) 23, 288, 309, 444, 554
TaqII GACCGA 1 cut(s) 941
TasI AATT 6 cut(s) 4, 113, 292, 375, 691, 784
TauI GCSGC 7 cut(s) 159, 200, 225, 397, 400, 597, 636
TfiI GAWTC 4 cut(s) 127, 467, 526, 586
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 255, 482
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 4, 163
TspRI CASTG 1 cut(s) 513
Tth111I GACNNNGTC 1 cut(s) 38
Van91I CCANNNNNTGG 1 cut(s) 244
VpaK11BI GGWCC 3 cut(s) 228, 768, 924
XapI RAATTY 4 cut(s) 4, 113, 292, 375
XceI RCATGY 2 cut(s) 700, 911
XhoI CTCGAG 1 cut(s) 308
XmnI GAANNNNTTC 1 cut(s) 53
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.