RLG00000012709

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003058
Physical Location & Seq
Forward (+)
21528084 .. 21530449
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012709

Sequence Viewer

Length: 846 bp
ATGTTCTTGGATCGGCCACGGCGTTGCTGCAGCGCGTTTTGTCTGATGGTTGTTCTTTTTCTTGTGATGATCGAGCATGAAGTGGTGAAGGAACACGAGACGGTTGCGAAGGCTGTGCTTCCGGTGATTCTATCTTGCATATTTTTGCTAGATTGCTTCGACATTCAACTAGTAGATGGTATTACCGAGGTCATTGCAGTTTTGATGATTGGTTGGATTTCTGTGTTTCAGCCCAATCTTACTCAGGCATGGCTTGATCCTTTGTCCGCTAATGTCGGAAGATATATGAGCCTCAACTTCAGGGCTTTGATCCTACATTGTTTTCAGTTGGGTGGGCAGCTTTTACTTGTCATGGACAAATGGATCCCAGGTTTGCTTGGTGGAAAATTGGGGGCTTCTAATCTTGCAGCAGATGATTGTGATCCTTATCAGAAAGTGGTTGATCCAATTGATTGTAATTCACTATGTTGGAATCTTGATCCTATTCTTCAACATTTATCATTGGATGAAGTCTCTGCCATTGAAGCAATGCGTTTATCTTCCATGTGGGAACCAGATGAACGGTATGGCCTTTTGAGAAAGAAAAAAAACGCTGGTGTCTGGTTTGGCCTTCCAATCAATTATAAGGTGGATTTGTCTTCTATTTCTACCTTTGATTCATGGTTTAATGGTGTAAGTACTTCATTGCAGCAGAATAAGATTTCTTTTACATTGTGGAATATCTGGATGGAGCGTTGTGCATTTATCTATGAGCATAAGGAGCCTAAAGCCTGGGGTGTGATTGCTAGGATCCTAAGTGCTATTAATGAATTTTTTGAAGTTTTGGTGTCACCCCAAAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

282

Amino Acids

31.91

Weight (kDa)

5.06

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AccII CGCG 1 cut(s) 35
AciI CCGC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 1 cut(s) 809
AcuI CTGAAG 1 cut(s) 283
AfaI GTAC 1 cut(s) 679
AgsI TTSAA 4 cut(s) 167, 491, 524, 818
AhlI ACTAGT 1 cut(s) 169
AjnI CCWGG 2 cut(s) 367, 770
AluBI AGCT 1 cut(s) 340
AluI AGCT 1 cut(s) 340
Alw26I GTCTC 2 cut(s) 92, 517
AoxI GGCC 3 cut(s) 14, 568, 607
ApeKI GCWGC 5 cut(s) 27, 30, 337, 407, 688
ApoI RAATTY 1 cut(s) 809
AseI ATTAAT 1 cut(s) 804
AspLEI GCGC 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 97, 136, 822
BamHI GGATCC 2 cut(s) 363, 789
BauI CACGAG 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 5 cut(s) 14, 42, 349, 419, 700
BccI CCATC 3 cut(s) 40, 170, 721
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 6 cut(s) 86, 97, 120, 131, 176, 210
BciT130I CCWGG 2 cut(s) 369, 772
BcoDI GTCTC 2 cut(s) 92, 517
BcuI ACTAGT 1 cut(s) 169
BfaI CTAG 3 cut(s) 149, 170, 786
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 5 cut(s) 28, 31, 338, 408, 689
BlsI GCNGC 5 cut(s) 29, 32, 339, 409, 690
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 2 cut(s) 369, 772
BmiI GGNNCC 4 cut(s) 365, 552, 762, 791
BmrFI CCNGG 2 cut(s) 369, 772
BpiI GAAGAC 1 cut(s) 630
BsaBI GATNNNNATC 2 cut(s) 420, 426
BsaJI CCNNGG 4 cut(s) 17, 186, 367, 771
BsaWI WCCGGW 1 cut(s) 121
Bse3DI GCAATG 3 cut(s) 192, 534, 683
Bse8I GATNNNNATC 2 cut(s) 420, 426
BseBI CCWGG 2 cut(s) 369, 772
BseDI CCNNGG 4 cut(s) 17, 186, 367, 771
BseGI GGATG 2 cut(s) 511, 732
BseJI GATNNNNATC 2 cut(s) 420, 426
BseMI GCAATG 3 cut(s) 192, 534, 683
BseMII CTCAG 1 cut(s) 257
BseXI GCAGC 5 cut(s) 14, 42, 349, 419, 700
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 3 cut(s) 16, 570, 609
BsiSI CCGG 1 cut(s) 122
BsmAI GTCTC 2 cut(s) 92, 517
BsmBI CGTCTC 1 cut(s) 92
BsnI GGCC 3 cut(s) 16, 570, 609
Bsp143I GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
BspACI CCGC 1 cut(s) 267
BspANI GGCC 3 cut(s) 16, 570, 609
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 4 cut(s) 365, 552, 762, 791
BspMAI CTGCAG 1 cut(s) 32
BsrDI GCAATG 3 cut(s) 192, 534, 683
BssECI CCNNGG 4 cut(s) 17, 186, 367, 771
BssMI GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
BssSI CACGAG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 369, 772
Bst4CI ACNGT 2 cut(s) 103, 564
BstDEI CTNAG 2 cut(s) 243, 794
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 2 cut(s) 511, 732
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 9 cut(s) 13, 72, 259, 312, 366, 424, 445, 481, 792
BstMAI GTCTC 2 cut(s) 92, 517
BstMBI GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
BstMWI GCNNNNNNNGC 2 cut(s) 524, 760
BstNI CCWGG 2 cut(s) 369, 772
BstSCI CCNGG 2 cut(s) 367, 770
BstSFI CTRYAG 1 cut(s) 28
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 5 cut(s) 14, 42, 349, 419, 700
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 2 cut(s) 363, 789
BstYI RGATCY 2 cut(s) 363, 789
BsuRI GGCC 3 cut(s) 16, 570, 609
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 2 cut(s) 511, 732
CfoI GCGC 1 cut(s) 35
Csp6I GTAC 1 cut(s) 678
CviAII CATG 5 cut(s) 77, 249, 352, 544, 660
CviQI GTAC 1 cut(s) 678
DdeI CTNAG 2 cut(s) 243, 794
DpnI GATC 9 cut(s) 12, 71, 258, 311, 365, 423, 444, 480, 791
DpnII GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
EaeI YGGCCR 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 367, 770
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 5 cut(s) 80, 252, 355, 547, 663
FatI CATG 5 cut(s) 76, 248, 351, 543, 659
Fnu4HI GCNGC 5 cut(s) 28, 31, 338, 408, 689
FokI GGATG 2 cut(s) 518, 739
Fsp4HI GCNGC 5 cut(s) 28, 31, 338, 408, 689
FspBI CTAG 3 cut(s) 149, 170, 786
GlaI GCGC 1 cut(s) 34
GluI GCNGC 5 cut(s) 28, 31, 338, 408, 689
HaeIII GGCC 3 cut(s) 16, 570, 609
HapII CCGG 1 cut(s) 122
HhaI GCGC 1 cut(s) 35
Hin1II CATG 5 cut(s) 80, 252, 355, 547, 663
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 3 cut(s) 127, 472, 656
HpaII CCGG 1 cut(s) 122
HphI GGTGA 3 cut(s) 97, 136, 822
Hpy188I TCNGA 3 cut(s) 45, 278, 432
Hpy188III TCNNGA 2 cut(s) 476, 724
HpyAV CCTTC 3 cut(s) 82, 103, 620
HpyCH4III ACNGT 2 cut(s) 103, 564
HpyCH4V TGCA 6 cut(s) 30, 138, 197, 407, 688, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 760
HpyF3I CTNAG 2 cut(s) 243, 794
Hsp92II CATG 5 cut(s) 80, 252, 355, 547, 663
HspAI GCGC 1 cut(s) 33
Kzo9I GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
LmnI GCTCC 2 cut(s) 730, 760
Lsp1109I GCAGC 5 cut(s) 14, 42, 349, 419, 700
MaeI CTAG 3 cut(s) 149, 170, 786
MaeIII GTNAC 1 cut(s) 828
MalI GATC 9 cut(s) 12, 71, 258, 311, 365, 423, 444, 480, 791
MboI GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
MboII GAAGA 4 cut(s) 291, 479, 531, 630
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 2 cut(s) 363, 789
MluCI AATT 5 cut(s) 386, 447, 457, 619, 809
MmeI TCCRAC 3 cut(s) 194, 256, 449
MnlI CCTC 2 cut(s) 181, 302
MseI TTAA 2 cut(s) 666, 804
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 2 cut(s) 369, 772
MunI CAATTG 1 cut(s) 447
MvaI CCWGG 2 cut(s) 369, 772
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 524, 760
NdeII GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
NlaIII CATG 5 cut(s) 80, 252, 355, 547, 663
NlaIV GGNNCC 4 cut(s) 365, 552, 762, 791
NmuCI GTSAC 1 cut(s) 828
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PfeI GAWTC 3 cut(s) 127, 472, 656
PkrI GCNGC 5 cut(s) 29, 32, 339, 409, 690
PshBI ATTAAT 1 cut(s) 804
PsiI TTATAA 1 cut(s) 624
Psp6I CCWGG 2 cut(s) 367, 770
PspGI CCWGG 2 cut(s) 367, 770
PspN4I GGNNCC 4 cut(s) 365, 552, 762, 791
PstI CTGCAG 1 cut(s) 32
PsuI RGATCY 2 cut(s) 363, 789
RsaI GTAC 1 cut(s) 679
RsaNI GTAC 1 cut(s) 678
SaqAI TTAA 2 cut(s) 666, 804
SatI GCNGC 5 cut(s) 28, 31, 338, 408, 689
Sau3AI GATC 9 cut(s) 10, 69, 256, 309, 363, 421, 442, 478, 789
ScaI AGTACT 1 cut(s) 679
ScrFI CCNGG 2 cut(s) 369, 772
SetI ASST 5 cut(s) 192, 342, 373, 630, 653
SfcI CTRYAG 1 cut(s) 28
SpeI ACTAGT 1 cut(s) 169
Sse9I AATT 5 cut(s) 386, 447, 457, 619, 809
SsiI CCGC 1 cut(s) 267
SspMI CTAG 3 cut(s) 149, 170, 786
StyD4I CCNGG 2 cut(s) 367, 770
TaaI ACNGT 2 cut(s) 103, 564
TaqI TCGA 2 cut(s) 72, 159
TasI AATT 5 cut(s) 386, 447, 457, 619, 809
TatI WGTACW 1 cut(s) 677
TfiI GAWTC 3 cut(s) 127, 472, 656
Tru1I TTAA 2 cut(s) 666, 804
Tru9I TTAA 2 cut(s) 666, 804
TseFI GTSAC 1 cut(s) 828
TseI GCWGC 5 cut(s) 27, 30, 337, 407, 688
Tsp45I GTSAC 1 cut(s) 828
TspDTI ATGAA 6 cut(s) 93, 522, 573, 648, 672, 822
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 1 cut(s) 809
XspI CTAG 3 cut(s) 149, 170, 786
ZrmI AGTACT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.