Rorug06G0151800

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003058
Physical Location & Seq
Reverse (-)
21729135 .. 21731446
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0151800.1

Sequence Viewer

Length: 444 bp
ATGGGGAATTTGGTGTCCGAAATTGGTCAAGGAGAATTGAAGCTTTTACTAGGAAAATGTGGGAGGATTCATCAGGAAAGTGAGGCGCTTTCTGGTGCTGTCGTGATAAGTGAATATGAGTTGCATGTCGATATGTACCTCCCAAGCTTGGAGACAACACTAAGGAATTTTTCCTGCTTGACCATTGGAGATGGTATTATGGTAGCATACAACAACAAGAAGTATTATATAGATATCATTGAATCAAAGCCTTCTAATGCAATAAGTATAATTGAGAGAGACTGTGAGGTGGACTTTGCACCTCCTCTTGATTATAAGGAACCTGAAAGGCTTGTGGCACCTGTTCCTCTTAACAAGGCAACAGCTCAAGTTGAAGAGGACCCTGCTGCGACTGAACCAAAATTCGATTCTGTTTTAGAATATGAGATTCAAAGAAGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

147

Amino Acids

16.45

Weight (kDa)

4.58

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFD1_N2 PF24842 50 - 102 2.3e-21 Ubiquitin fusion degradation protein UFD1, N-terminal subdomain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 337
AcsI RAATTY 3 cut(s) 7, 166, 401
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 4 cut(s) 40, 242, 374, 431
AjuI GAANNNNNNNTTGG 2 cut(s) 391, 423
AluBI AGCT 3 cut(s) 43, 147, 365
AluI AGCT 3 cut(s) 43, 147, 365
Alw26I GTCTC 2 cut(s) 146, 273
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 3 cut(s) 7, 166, 401
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 379
AvaII GGWCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 146, 273
BfaI CTAG 1 cut(s) 50
BfoI RGCGCY 1 cut(s) 89
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 3 cut(s) 321, 339, 381
BpuEI CTTGAG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 148
BseLI CCNNNNNNNGG 1 cut(s) 148
BseRI GAGGAG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 373
BshNI GGYRCC 1 cut(s) 337
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 2 cut(s) 146, 273
BspLI GGNNCC 3 cut(s) 321, 339, 381
BspT107I GGYRCC 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 284
Bst6I CTCTTC 1 cut(s) 369
BstDEI CTNAG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 1 cut(s) 88
BstMAI GTCTC 2 cut(s) 146, 273
BstNSI RCATGY 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 373
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 136
CviAII CATG 1 cut(s) 125
CviJI RGCY 5 cut(s) 43, 147, 250, 331, 365
CviKI_1 RGCY 5 cut(s) 43, 147, 250, 331, 365
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 379
EcoRV GATATC 1 cut(s) 235
FaeI CATG 1 cut(s) 128
FatI CATG 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 87
GluI GCNGC 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 89
HhaI GCGC 1 cut(s) 88
Hin1II CATG 1 cut(s) 128
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HindIII AAGCTT 2 cut(s) 41, 145
HinfI GANTC 4 cut(s) 67, 242, 407, 427
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 3 cut(s) 74, 103, 308
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 1 cut(s) 261
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4V TGCA 3 cut(s) 124, 260, 299
HpyF3I CTNAG 1 cut(s) 161
Hsp92II CATG 1 cut(s) 128
HspAI GCGC 1 cut(s) 86
LpnPI CCDG 6 cut(s) 59, 78, 187, 336, 354, 396
Lsp1109I GCAGC 1 cut(s) 373
MaeI CTAG 1 cut(s) 50
MboII GAAGA 1 cut(s) 386
MluCI AATT 6 cut(s) 7, 21, 35, 166, 270, 401
MnlI CCTC 8 cut(s) 57, 76, 149, 280, 312, 315, 357, 370
MseI TTAA 1 cut(s) 351
NlaIII CATG 1 cut(s) 128
NlaIV GGNNCC 3 cut(s) 321, 339, 381
NspI RCATGY 1 cut(s) 128
PfeI GAWTC 4 cut(s) 67, 242, 407, 427
PkrI GCNGC 1 cut(s) 388
PpuMI RGGWCCY 1 cut(s) 379
PsiI TTATAA 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 379
PspN4I GGNNCC 3 cut(s) 321, 339, 381
PspPI GGNCC 1 cut(s) 379
PspPPI RGGWCCY 1 cut(s) 379
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 387
Sau96I GGNCC 1 cut(s) 379
SetI ASST 8 cut(s) 45, 141, 149, 291, 304, 325, 343, 367
SinI GGWCC 1 cut(s) 379
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 6 cut(s) 7, 21, 35, 166, 270, 401
SspMI CTAG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 284
TaqI TCGA 2 cut(s) 129, 405
TasI AATT 6 cut(s) 7, 21, 35, 166, 270, 401
TfiI GAWTC 4 cut(s) 67, 242, 407, 427
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 3 cut(s) 7, 166, 401
XceI RCATGY 1 cut(s) 128
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.