Rw6G022900

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003058
Physical Location & Seq
Reverse (-)
45104136 .. 45105413
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022900.1

Sequence Viewer

Length: 1278 bp
ATGCTCTTGAGTCTTGACCATGACGTCCTTTCCGCAATCTTCTCTTTGCTTGACTGCTGCCAAGACCTCGTCAGTTGTTCCTTGGTTTGCAAAACCTGGAGGACCGCCATCGCCAAATGTAAGTGTTTCGAAGCGCTTTATTACAAGCAGTGGCAAATGGGTCTTGTGAGGGGTCTTTGTTTGGAGGAGATGGCCGGTGTGACTCATGGACTTGCATTGCTTCAAAGCCGTCGGATTAGTGTTGATCACTGGAAGGGTCACTCTGCTAATATTGATCAATGCCGAAACAAGGATGGGTTGCTTCTCACTGGCAGCAGCAGGAAGAAGTTCATGCGTCTGTGGTCTCTGGAAGAAAGCCGTAAATGTGTGTATAAATTTCAAGGAGAATATCGAGTTCCAGTAGACTATGGCCCTTTGGTTGATTTTGACTTTGATGAGAGCAAGATTGTTGGCTTGGTCGGCTCAAAGAATAGTCGAATATGCATATGGAGGCACAGTGGGATGACTAGGATATTAAACCCCTTTAAGTGCAAGGGTACCTTTCCGAAGGGTTTATGCATGAGCTACTGTGATCCAGAGGCTGTGGTTGGATGTGCTGACGGCACTGCTCGGGTGTTTGACATGTACAGTAGCCAATGTTCTCAAATTCTTAGAATGAACGACCCTGAGGTGCCAGTGACATGCCTAGGGTTGAGTGATGAAGGCAGTCGGTTGTCCATTATTGCTGGTGGTGGTTCTTCTATCACAGTTTCCTCTGTACTTTTACCTGATCATGAGTTTGCAGCAAAATTGCATATAGCAGGCGAGATAAAGACTTTGAGTTACAACCCTTGCGGCAATGATAGGACAGTGTTTGCTGGATCAAGCAATGGATATGTGTACGGTTGGGACCTGAGGACATGTAGACTTTTGTGGGAAACGAGAGTGAGTCGGGGTGCTGTGTGTTCAATGCAGCACCGGAGGAATGATGCATCAACTTTGGTTGTGGGTGGAATGGATGGTGTGCTTCATCTAGTGGATCAGAACACAGGGAAAGTGGTTACTAAAATTGTCTTGGACACCAGCTACTGTGTACTGCCAAGTTCTAAACCTGCTTCAAGTACAAGTGGTGGTTGTTATCAAAACATAGTAATTCAGAGATTGAGAGGAAAAAGGATTTTACCTGATGCCGACATTGTCAGCATTCCTGCACCTCCCATCACCTGCTTGGCAGTGGGGATGAAGAAGGTTATCACCACACACGATAGGGACTACATCAGAACCTGGAAATTTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

425

Amino Acids

47.06

Weight (kDa)

8.76

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 42 3.9e-07 F-box domain
F-box-like PF12937 7 - 38 5.6e-07 F-box-like
Beta-prop_WDR3_1st PF25173 193 - 337 1e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 202 - 345 3.7e-07 CDC20/Fizzy WD40 domain
Beta-prop_ACSF4 PF13570 257 - 350 4.5e-07 Beta-alanine-activating enzyme, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1211
AasI GACNNNNNNGTC 1 cut(s) 23
AatII GACGTC 1 cut(s) 27
Acc36I ACCTGC 2 cut(s) 1099, 1211
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 2 cut(s) 536, 670
AccI GTMKAC 2 cut(s) 402, 904
AciI CCGC 3 cut(s) 33, 105, 834
AclWI GGATC 3 cut(s) 566, 868, 1026
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 3 cut(s) 374, 645, 1268
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 6 cut(s) 538, 626, 759, 881, 1074, 1102
AfeI AGCGCT 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 2 cut(s) 621, 899
AgsI TTSAA 4 cut(s) 224, 380, 948, 1098
AjnI CCWGG 2 cut(s) 95, 1262
AluBI AGCT 2 cut(s) 564, 1065
AluI AGCT 2 cut(s) 564, 1065
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 3 cut(s) 566, 868, 1026
AlwNI CAGNNNCTG 3 cut(s) 581, 1068, 1263
Ama87I CYCGRG 1 cut(s) 609
Aor51HI AGCGCT 1 cut(s) 135
AoxI GGCC 2 cut(s) 192, 409
ApeKI GCWGC 5 cut(s) 57, 312, 315, 782, 952
ApoI RAATTY 3 cut(s) 374, 645, 1268
Asp700I GAANNNNTTC 1 cut(s) 326
Asp718I GGTACC 1 cut(s) 536
AspA2I CCTAGG 1 cut(s) 685
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 3 cut(s) 102, 410, 889
AsuHPI GGTGA 2 cut(s) 1192, 1225
AsuII TTCGAA 1 cut(s) 129
AvaI CYCGRG 1 cut(s) 609
AvaII GGWCC 2 cut(s) 102, 889
AvrII CCTAGG 1 cut(s) 685
AxyI CCTNAGG 2 cut(s) 666, 893
BanI GGYRCC 2 cut(s) 536, 670
BbvI GCAGC 5 cut(s) 44, 324, 327, 794, 964
BccI CCATC 5 cut(s) 116, 184, 287, 992, 1205
BceAI ACGGC 3 cut(s) 213, 342, 616
BciT130I CCWGG 2 cut(s) 97, 1264
BclI TGATCA 3 cut(s) 244, 274, 769
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 4 cut(s) 507, 686, 1013, 1276
BfoI RGCGCY 1 cut(s) 137
BfuAI ACCTGC 2 cut(s) 1099, 1211
BisI GCNGC 6 cut(s) 58, 313, 316, 783, 835, 953
BlnI CCTAGG 1 cut(s) 685
BlsI GCNGC 6 cut(s) 59, 314, 317, 784, 836, 954
Bme1390I CCNGG 2 cut(s) 97, 1264
Bme18I GGWCC 2 cut(s) 102, 889
BmeT110I CYCGRG 1 cut(s) 609
BmgT120I GGNCC 3 cut(s) 102, 410, 889
BmiI GGNNCC 3 cut(s) 538, 672, 890
BmrFI CCNGG 2 cut(s) 97, 1264
BmsI GCATC 3 cut(s) 958, 980, 1156
BpmI CTGGAG 1 cut(s) 118
Bpu14I TTCGAA 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 81, 685
BsaWI WCCGGW 1 cut(s) 957
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 4 cut(s) 254, 313, 398, 674
Bse21I CCTNAGG 2 cut(s) 666, 893
Bse3DI GCAATG 3 cut(s) 215, 844, 874
BseBI CCWGG 2 cut(s) 97, 1264
BseDI CCNNGG 2 cut(s) 81, 685
BseGI GGATG 5 cut(s) 298, 507, 596, 1003, 1224
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 3 cut(s) 215, 844, 874
BseMII CTCAG 2 cut(s) 657, 884
BseNI ACTGG 4 cut(s) 254, 313, 398, 674
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 5 cut(s) 44, 324, 327, 794, 964
BsgI GTGCAG 1 cut(s) 1173
BshFI GGCC 2 cut(s) 194, 411
BshNI GGYRCC 2 cut(s) 536, 670
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 2 cut(s) 195, 958
BslFI GGGAC 2 cut(s) 902, 1262
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 348
BsmFI GGGAC 2 cut(s) 902, 1262
BsmI GAATGC 1 cut(s) 1182
BsnI GGCC 2 cut(s) 194, 411
Bso31I GGTCTC 1 cut(s) 348
BsoBI CYCGRG 1 cut(s) 609
Bsp119I TTCGAA 1 cut(s) 129
Bsp1407I TGTACA 1 cut(s) 624
Bsp143I GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
BspACI CCGC 3 cut(s) 33, 105, 834
BspANI GGCC 2 cut(s) 194, 411
BspCNI CTCAG 2 cut(s) 658, 885
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 3 cut(s) 538, 672, 890
BspMI ACCTGC 2 cut(s) 1099, 1211
BspPI GGATC 3 cut(s) 566, 868, 1026
BspT104I TTCGAA 1 cut(s) 129
BspT107I GGYRCC 2 cut(s) 536, 670
BspTNI GGTCTC 1 cut(s) 348
BsrDI GCAATG 3 cut(s) 215, 844, 874
BsrFI RCCGGY 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 4 cut(s) 254, 313, 398, 674
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 81, 685
BssMI GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 2 cut(s) 81, 685
Bst2UI CCWGG 2 cut(s) 97, 1264
Bst4CI ACNGT 7 cut(s) 497, 569, 629, 748, 850, 884, 1070
BstACI GRCGYC 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 624
BstBI TTCGAA 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 3 cut(s) 650, 666, 893
BstF5I GGATG 5 cut(s) 298, 507, 596, 1003, 1224
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 6 cut(s) 247, 277, 574, 772, 863, 1021
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
BstMWI GCNNNNNNNGC 1 cut(s) 459
BstNI CCWGG 2 cut(s) 97, 1264
BstNSI RCATGY 3 cut(s) 625, 684, 903
BstSCI CCNGG 2 cut(s) 95, 1262
BstV1I GCAGC 5 cut(s) 44, 324, 327, 794, 964
Bsu36I CCTNAGG 2 cut(s) 666, 893
BsuRI GGCC 2 cut(s) 194, 411
BtgZI GCGATG 1 cut(s) 94
BtsCI GGATG 5 cut(s) 298, 507, 596, 1003, 1224
BtsI GCAGTG 3 cut(s) 155, 603, 1218
BtsIMutI CAGTG 8 cut(s) 155, 247, 306, 502, 603, 681, 855, 1218
BveI ACCTGC 2 cut(s) 1099, 1211
Cac8I GCNNGC 1 cut(s) 802
CaiI CAGNNNCTG 3 cut(s) 581, 1068, 1263
CciI TCATGA 1 cut(s) 772
CfoI GCGC 1 cut(s) 136
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 3 cut(s) 102, 410, 889
CseI GACGC 1 cut(s) 323
Csp6I GTAC 6 cut(s) 537, 625, 758, 880, 1073, 1101
CviAII CATG 8 cut(s) 20, 206, 331, 559, 622, 681, 773, 900
CviQI GTAC 6 cut(s) 537, 625, 758, 880, 1073, 1101
DdeI CTNAG 3 cut(s) 650, 666, 893
DpnI GATC 6 cut(s) 246, 276, 573, 771, 862, 1020
DpnII GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
EaeI YGGCCR 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 81, 685
Eco31I GGTCTC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 102, 889
Eco47III AGCGCT 1 cut(s) 135
Eco81I CCTNAGG 2 cut(s) 666, 893
Eco88I CYCGRG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 889
EcoRII CCWGG 2 cut(s) 95, 1262
EcoT14I CCWWGG 2 cut(s) 81, 685
EcoT22I ATGCAT 3 cut(s) 485, 560, 973
ErhI CCWWGG 2 cut(s) 81, 685
FaeI CATG 8 cut(s) 23, 209, 334, 562, 625, 684, 776, 903
FalI AAGNNNNNCTT 2 cut(s) 524, 556
FaqI GGGAC 2 cut(s) 902, 1262
FatI CATG 8 cut(s) 19, 205, 330, 558, 621, 680, 772, 899
FauNDI CATATG 1 cut(s) 485
FbaI TGATCA 3 cut(s) 244, 274, 769
FblI GTMKAC 2 cut(s) 402, 904
Fnu4HI GCNGC 6 cut(s) 58, 313, 316, 783, 835, 953
FokI GGATG 5 cut(s) 305, 514, 603, 1010, 1231
Fsp4HI GCNGC 6 cut(s) 58, 313, 316, 783, 835, 953
FspBI CTAG 4 cut(s) 507, 686, 1013, 1276
GlaI GCGC 1 cut(s) 135
GluI GCNGC 6 cut(s) 58, 313, 316, 783, 835, 953
GsuI CTGGAG 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 2 cut(s) 194, 411
HapII CCGG 2 cut(s) 195, 958
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 8 cut(s) 23, 209, 334, 562, 625, 684, 776, 903
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 3 cut(s) 10, 202, 928
HpaII CCGG 2 cut(s) 195, 958
HphI GGTGA 2 cut(s) 1192, 1225
Hpy166II GTNNAC 4 cut(s) 403, 880, 905, 1073
Hpy188I TCNGA 5 cut(s) 234, 546, 1023, 1137, 1259
Hpy188III TCNNGA 5 cut(s) 7, 14, 347, 575, 773
Hpy8I GTNNAC 4 cut(s) 403, 880, 905, 1073
Hpy99I CGWCG 1 cut(s) 234
HpyAV CCTTC 4 cut(s) 247, 541, 695, 1219
HpyCH4III ACNGT 7 cut(s) 497, 569, 629, 748, 850, 884, 1070
HpyCH4IV ACGT 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 1 cut(s) 459
HpyF3I CTNAG 3 cut(s) 650, 666, 893
HpySE526I ACGT 1 cut(s) 24
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 8 cut(s) 23, 209, 334, 562, 625, 684, 776, 903
HspAI GCGC 1 cut(s) 134
KpnI GGTACC 1 cut(s) 540
Ksp22I TGATCA 3 cut(s) 244, 274, 769
Kzo9I GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
Lsp1109I GCAGC 5 cut(s) 44, 324, 327, 794, 964
LweI GCATC 3 cut(s) 958, 980, 1156
MaeI CTAG 4 cut(s) 507, 686, 1013, 1276
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 5 cut(s) 199, 257, 676, 821, 1039
MalI GATC 6 cut(s) 246, 276, 573, 771, 862, 1020
MboI GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
MboII GAAGA 5 cut(s) 31, 334, 362, 729, 1234
MluCI AATT 6 cut(s) 374, 645, 788, 1047, 1131, 1268
MlyI GAGTC 3 cut(s) 19, 196, 937
MmeI TCCRAC 2 cut(s) 212, 568
Mph1103I ATGCAT 3 cut(s) 485, 560, 973
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 3 cut(s) 515, 525, 1272
MspI CCGG 2 cut(s) 195, 958
MspR9I CCNGG 2 cut(s) 97, 1264
Mva1269I GAATGC 1 cut(s) 1182
MvaI CCWGG 2 cut(s) 97, 1264
MwoI GCNNNNNNNGC 1 cut(s) 459
NdeI CATATG 1 cut(s) 485
NdeII GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
NlaIII CATG 8 cut(s) 23, 209, 334, 562, 625, 684, 776, 903
NlaIV GGNNCC 3 cut(s) 538, 672, 890
NmuCI GTSAC 3 cut(s) 199, 257, 676
NsiI ATGCAT 3 cut(s) 485, 560, 973
NspI RCATGY 3 cut(s) 625, 684, 903
NspV TTCGAA 1 cut(s) 129
PagI TCATGA 1 cut(s) 772
PaqCI CACCTGC 1 cut(s) 1211
PciI ACATGT 2 cut(s) 621, 899
PctI GAATGC 1 cut(s) 1182
PdmI GAANNNNTTC 1 cut(s) 326
PflFI GACNNNGTC 2 cut(s) 68, 1175
PkrI GCNGC 6 cut(s) 59, 314, 317, 784, 836, 954
PleI GAGTC 3 cut(s) 18, 196, 936
PpsI GAGTC 3 cut(s) 18, 196, 936
PpuMI RGGWCCY 1 cut(s) 889
PscI ACATGT 2 cut(s) 621, 899
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 2 cut(s) 95, 1262
PspGI CCWGG 2 cut(s) 95, 1262
PspN4I GGNNCC 3 cut(s) 538, 672, 890
PspPI GGNCC 3 cut(s) 102, 410, 889
PspPPI RGGWCCY 1 cut(s) 889
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
PstNI CAGNNNCTG 3 cut(s) 581, 1068, 1263
PsyI GACNNNGTC 2 cut(s) 68, 1175
RsaI GTAC 6 cut(s) 538, 626, 759, 881, 1074, 1102
RsaNI GTAC 6 cut(s) 537, 625, 758, 880, 1073, 1101
SaqAI TTAA 3 cut(s) 515, 525, 1272
SatI GCNGC 6 cut(s) 58, 313, 316, 783, 835, 953
Sau3AI GATC 6 cut(s) 244, 274, 571, 769, 860, 1018
Sau96I GGNCC 3 cut(s) 102, 410, 889
SchI GAGTC 3 cut(s) 19, 196, 937
ScrFI CCNGG 2 cut(s) 97, 1264
SfaNI GCATC 3 cut(s) 958, 980, 1156
SfuI TTCGAA 1 cut(s) 129
SinI GGWCC 2 cut(s) 102, 889
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 6 cut(s) 374, 645, 788, 1047, 1131, 1268
SsiI CCGC 3 cut(s) 33, 105, 834
SspI AATATT 1 cut(s) 271
SspMI CTAG 4 cut(s) 507, 686, 1013, 1276
StyD4I CCNGG 2 cut(s) 95, 1262
StyI CCWWGG 2 cut(s) 81, 685
TaaI ACNGT 7 cut(s) 497, 569, 629, 748, 850, 884, 1070
TaiI ACGT 1 cut(s) 27
TaqI TCGA 3 cut(s) 129, 391, 475
TasI AATT 6 cut(s) 374, 645, 788, 1047, 1131, 1268
TatI WGTACW 4 cut(s) 624, 757, 1072, 1100
TauI GCSGC 1 cut(s) 837
Tru1I TTAA 3 cut(s) 515, 525, 1272
Tru9I TTAA 3 cut(s) 515, 525, 1272
TscAI CASTG 8 cut(s) 155, 254, 313, 502, 610, 681, 855, 1218
TseFI GTSAC 3 cut(s) 199, 257, 676
TseI GCWGC 5 cut(s) 57, 312, 315, 782, 952
Tsp45I GTSAC 3 cut(s) 199, 257, 676
TspDTI ATGAA 5 cut(s) 319, 671, 714, 998, 1235
TspRI CASTG 8 cut(s) 155, 254, 313, 502, 610, 681, 855, 1218
Tth111I GACNNNGTC 2 cut(s) 68, 1175
VpaK11BI GGWCC 2 cut(s) 102, 889
XapI RAATTY 3 cut(s) 374, 645, 1268
XceI RCATGY 3 cut(s) 625, 684, 903
XcmI CCANNNNNNNNNTGG 1 cut(s) 1204
XmaJI CCTAGG 1 cut(s) 685
XmiI GTMKAC 2 cut(s) 402, 904
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 4 cut(s) 507, 686, 1013, 1276
ZraI GACGTC 1 cut(s) 25
Zsp2I ATGCAT 3 cut(s) 485, 560, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.