RLG00000013135

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006430
Physical Location & Seq
Forward (+)
26670781 .. 26671454
674 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013135

Sequence Viewer

Length: 513 bp
ATGGACCCTAAGCTCACTCGAACTCGCCACCATTCTGGAGCTCTTCCTTCATCTCCCTCTCTCTCCCTCCCTAGTTTCACAGGTAGCTGGTTCACACAAATCGAGCCATGGCTCCTCCCAGTATTCTCGGTCCTCCCGAGTTCCACAAACCCTCACCCACCACCACCGCTCCGCGAGCCACCCCAACCCAAATTCAAACCCGACTCCAAACCCACCACCGGCGAATCTTTCGTGGACCTGATGGTGGCGAATTTCAACGACGTCGGAACCAAGCACGACCTCCCAATGGGCCTGACGGAGAACGACTCTGCCACGTTTCTCTCCACCGGCAACCCCTGCCTCGATCTCTTCTTCCACGTCATCCCAAACACTCCAGTCACGCCCTTGAGTGGGAAGAAGCACATGGCTCACACAATTGCTCACACCACTCTCGGCCTCACTCAGATTTCTTCTGTCTTTGAAGCCCCAAAGGTTTTGCTCTGCTCCGTTTCACTGAAATATTTGGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

171

Amino Acids

18.58

Weight (kDa)

6.69

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 1 cut(s) 174
AciI CCGC 2 cut(s) 167, 172
AcsI RAATTY 3 cut(s) 191, 250, 506
AcyI GRCGYC 1 cut(s) 261
AfiI CCNNNNNNNGG 5 cut(s) 218, 244, 286, 389, 390
AgsI TTSAA 3 cut(s) 196, 256, 461
AjiI CACGTC 1 cut(s) 358
AluBI AGCT 3 cut(s) 13, 41, 87
AluI AGCT 3 cut(s) 13, 41, 87
Alw21I GWGCWC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 2 cut(s) 289, 433
ApoI RAATTY 3 cut(s) 191, 250, 506
AspS9I GGNCC 4 cut(s) 4, 130, 235, 289
AsuHPI GGTGA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 3 cut(s) 4, 130, 235
BanII GRGCYC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 43
BccI CCATC 1 cut(s) 235
BfaI CTAG 1 cut(s) 72
Bme18I GGWCC 3 cut(s) 4, 130, 235
BmeT110I CYCGRG 1 cut(s) 136
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 4 cut(s) 4, 130, 235, 289
BmiI GGNNCC 3 cut(s) 6, 113, 268
BmrI ACTGGG 1 cut(s) 113
BmuI ACTGGG 1 cut(s) 113
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 57, 357
Bpu10I CCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 406
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 5 cut(s) 218, 244, 286, 389, 390
Bse118I RCCGGY 2 cut(s) 218, 326
Bse1I ACTGG 2 cut(s) 119, 374
BseDI CCNNGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 360
BseLI CCNNNNNNNGG 5 cut(s) 218, 244, 286, 389, 390
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 2 cut(s) 119, 374
BseRI GAGGAG 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 2 cut(s) 291, 435
BsiHKAI GWGCWC 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 2 cut(s) 219, 327
BslI CCNNNNNNNGG 5 cut(s) 218, 244, 286, 389, 390
BsnI GGCC 2 cut(s) 291, 435
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 1 cut(s) 343
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 2 cut(s) 167, 172
BspANI GGCC 2 cut(s) 291, 435
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 6, 113, 268
BspQI GCTCTTC 1 cut(s) 48
BsrBI CCGCTC 1 cut(s) 169
BsrFI RCCGGY 2 cut(s) 218, 326
BsrI ACTGG 2 cut(s) 119, 374
BssAI RCCGGY 2 cut(s) 218, 326
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 1 cut(s) 343
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 107
Bst6I CTCTTC 2 cut(s) 48, 353
BstACI GRCGYC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 176
BstDEI CTNAG 2 cut(s) 9, 441
BstDSI CCRYGG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 360
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 1 cut(s) 346
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 2 cut(s) 175, 336
BstUI CGCG 1 cut(s) 174
BstXI CCANNNNNNTGG 1 cut(s) 35
BsuRI GGCC 2 cut(s) 291, 435
BtgI CCRYGG 1 cut(s) 107
BtrI CACGTC 1 cut(s) 358
BtsCI GGATG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 491
Cac8I GCNNGC 1 cut(s) 176
Cfr10I RCCGGY 2 cut(s) 218, 326
Cfr13I GGNCC 4 cut(s) 4, 130, 235, 289
CviAII CATG 2 cut(s) 108, 403
DdeI CTNAG 2 cut(s) 9, 441
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 48, 353
EarI CTCTTC 2 cut(s) 48, 353
Ecl136II GAGCTC 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 3 cut(s) 4, 130, 235
Eco53kI GAGCTC 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 2 cut(s) 111, 406
FaiI YATR 2 cut(s) 109, 404
FatI CATG 2 cut(s) 107, 402
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 43
FspBI CTAG 1 cut(s) 72
GsuI CTGGAG 2 cut(s) 57, 357
HaeIII GGCC 2 cut(s) 291, 435
HapII CCGG 2 cut(s) 219, 327
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 2 cut(s) 111, 406
HinfI GANTC 3 cut(s) 203, 224, 305
HpaII CCGG 2 cut(s) 219, 327
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 93, 235
Hpy188I TCNGA 2 cut(s) 266, 444
Hpy188III TCNNGA 2 cut(s) 36, 136
Hpy8I GTNNAC 2 cut(s) 93, 235
Hpy99I CGWCG 2 cut(s) 263, 266
HpyAV CCTTC 1 cut(s) 57
HpyCH4IV ACGT 3 cut(s) 261, 314, 357
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 336
HpyF3I CTNAG 2 cut(s) 9, 441
HpySE526I ACGT 3 cut(s) 261, 314, 357
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 2 cut(s) 111, 406
Kzo9I GATC 1 cut(s) 343
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 4 cut(s) 38, 117, 174, 488
MaeI CTAG 1 cut(s) 72
MaeII ACGT 3 cut(s) 261, 314, 357
MaeIII GTNAC 1 cut(s) 376
MalI GATC 1 cut(s) 345
MbiI CCGCTC 1 cut(s) 169
MboI GATC 1 cut(s) 343
MboII GAAGA 5 cut(s) 35, 340, 343, 406, 441
MfeI CAATTG 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 4 cut(s) 191, 250, 414, 506
MlyI GAGTC 2 cut(s) 197, 299
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 8 cut(s) 67, 77, 125, 143, 162, 290, 350, 446
MseI TTAA 1 cut(s) 511
MspI CCGG 2 cut(s) 219, 327
MunI CAATTG 1 cut(s) 414
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 175, 336
NcoI CCATGG 1 cut(s) 107
NdeII GATC 1 cut(s) 343
NlaIII CATG 2 cut(s) 111, 406
NlaIV GGNNCC 3 cut(s) 6, 113, 268
NmeAIII GCCGAG 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 134
PfeI GAWTC 1 cut(s) 224
PleI GAGTC 2 cut(s) 197, 299
PpsI GAGTC 2 cut(s) 197, 299
Psp124BI GAGCTC 1 cut(s) 43
PspN4I GGNNCC 3 cut(s) 6, 113, 268
PspPI GGNCC 4 cut(s) 4, 130, 235, 289
SacI GAGCTC 1 cut(s) 43
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 511
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 4 cut(s) 4, 130, 235, 289
SchI GAGTC 2 cut(s) 197, 299
SduI GDGCHC 1 cut(s) 43
SgrAI CRCCGGYG 1 cut(s) 218
SinI GGWCC 3 cut(s) 4, 130, 235
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
Sse9I AATT 4 cut(s) 191, 250, 414, 506
SsiI CCGC 2 cut(s) 167, 172
SspI AATATT 1 cut(s) 500
SspMI CTAG 1 cut(s) 72
SstI GAGCTC 1 cut(s) 43
StyI CCWWGG 1 cut(s) 107
TaiI ACGT 3 cut(s) 264, 317, 360
TaqI TCGA 3 cut(s) 19, 102, 342
TaqII GACCGA 1 cut(s) 118
TasI AATT 4 cut(s) 191, 250, 414, 506
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 511
Tru9I TTAA 1 cut(s) 511
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 1 cut(s) 39
TspGWI ACGGA 2 cut(s) 311, 475
TspRI CASTG 1 cut(s) 498
VpaK11BI GGWCC 3 cut(s) 4, 130, 235
XapI RAATTY 3 cut(s) 191, 250, 506
XspI CTAG 1 cut(s) 72
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.