RLG00000020978

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010587
Physical Location & Seq
Reverse (-)
70687580 .. 70688315
736 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020978

Sequence Viewer

Length: 645 bp
ATGAAATTCAAGGCTTTCATTTTGCTGGGGCTTCTGTTTGCAGTAGTTCTCATCTCTTCCATTGACGCAGCGGCTGAGACATCCAAACATGAGAATAAAGTGGAAGCCAGGGTAGCCCCAAAAGAAAAGAAATCGAGCCAAGTAGAAGATTCAAAGCCGGTCGAAATTAGCCGATGCGGGTGCTGCTGTCGTCATAATATGTGGGGATGCGGTTTGCACTGTTGTGATGGTAGAGGACCTGGCACTGGCTGTCCACCTGGAGGCAGCACTACAGGTGTTGGCGCTATTCCAGATGTTGGAGGCGGCATTCCGGGTGTTGGAGGAGGCATTCCGGGCGTTGGAGGGGGCATTCCGGGTGTTGGAGGAGGCATTCCGGGCGTTGGAGGCGGCATTCCGGGCGTTGGAGGAGGCATTCCGGGAGGCGGCATTCCGGGCGTTGGAGGAGGCATTCCGGGCGTTGGAGAAGGCGTTCCAGGTGTTGGAGGCATTCCGGGGGTTGGCGGTGGGGTTCCAGGGATTGGCGGCATTCCAGGCGTTGGACGCGGTGTTCCAGGGGTTGGTGGAGGAATAGGAGGCGGCATTCCAGGCATGCCCGGCCGTGGAGGAATTGGCGGCGGCGGCCGAGTCTGTGGGCGTGGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

215

Amino Acids

19.59

Weight (kDa)

8.81

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRP PF07172 1 - 125 1.6e-06 Glycine rich protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 5 cut(s) 296, 479, 518, 536, 557
AccII CGCG 1 cut(s) 543
AcoI YGGCCR 2 cut(s) 595, 619
AcsI RAATTY 1 cut(s) 5
AgsI TTSAA 2 cut(s) 10, 153
AjnI CCWGG 8 cut(s) 107, 238, 256, 472, 511, 529, 550, 583
AleI CACNNNNGTG 1 cut(s) 222
AloI GAACNNNNNNTCC 4 cut(s) 453, 485, 531, 563
Alw26I GTCTC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 2 cut(s) 595, 619
ApeKI GCWGC 3 cut(s) 68, 183, 264
ApoI RAATTY 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 1 cut(s) 236
AvaII GGWCC 1 cut(s) 236
BbvI GCAGC 3 cut(s) 80, 170, 276
BccI CCATC 1 cut(s) 221
BceAI ACGGC 1 cut(s) 582
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BciT130I CCWGG 8 cut(s) 109, 240, 258, 474, 513, 531, 552, 585
BcoDI GTCTC 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 270
BfoI RGCGCY 1 cut(s) 285
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 1 cut(s) 510
BmsI GCATC 2 cut(s) 164, 197
BpmI CTGGAG 1 cut(s) 279
BsaJI CCNNGG 5 cut(s) 108, 491, 512, 551, 598
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 250
BseBI CCWGG 8 cut(s) 109, 240, 258, 474, 513, 531, 552, 585
BseDI CCNNGG 5 cut(s) 108, 491, 512, 551, 598
BseGI GGATG 2 cut(s) 80, 212
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 4 cut(s) 336, 378, 420, 456
BseX3I CGGCCG 2 cut(s) 595, 619
BseXI GCAGC 3 cut(s) 80, 170, 276
BseYI CCCAGC 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 543
Bsh1285I CGRYCG 3 cut(s) 162, 598, 622
BshFI GGCC 2 cut(s) 597, 621
BsiEI CGRYCG 3 cut(s) 162, 598, 622
BsmAI GTCTC 1 cut(s) 71
BsnI GGCC 2 cut(s) 597, 621
BspANI GGCC 2 cut(s) 597, 621
BspCNI CTCAG 1 cut(s) 67
BspFNI CGCG 1 cut(s) 543
BspLI GGNNCC 1 cut(s) 510
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 5 cut(s) 108, 491, 512, 551, 598
Bst2UI CCWGG 8 cut(s) 109, 240, 258, 474, 513, 531, 552, 585
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 590
BstDEI CTNAG 1 cut(s) 75
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 2 cut(s) 80, 212
BstFNI CGCG 1 cut(s) 543
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 1 cut(s) 284
BstMAI GTCTC 1 cut(s) 71
BstMCI CGRYCG 3 cut(s) 162, 598, 622
BstNI CCWGG 8 cut(s) 109, 240, 258, 474, 513, 531, 552, 585
BstNSI RCATGY 1 cut(s) 592
BstSFI CTRYAG 1 cut(s) 270
BstUI CGCG 1 cut(s) 543
BstV1I GCAGC 3 cut(s) 80, 170, 276
BstZI CGGCCG 2 cut(s) 595, 619
BsuRI GGCC 2 cut(s) 597, 621
BtgI CCRYGG 1 cut(s) 598
BtsCI GGATG 2 cut(s) 80, 212
BtsIMutI CAGTG 2 cut(s) 217, 243
Cac8I GCNNGC 1 cut(s) 590
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 236
CseI GACGC 2 cut(s) 74, 549
CviAII CATG 2 cut(s) 89, 589
DdeI CTNAG 1 cut(s) 75
EaeI YGGCCR 2 cut(s) 595, 619
EagI CGGCCG 2 cut(s) 595, 619
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
EclXI CGGCCG 2 cut(s) 595, 619
Eco47I GGWCC 1 cut(s) 236
Eco52I CGGCCG 2 cut(s) 595, 619
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 8 cut(s) 107, 238, 256, 472, 511, 529, 550, 583
FaeI CATG 2 cut(s) 92, 592
FaiI YATR 4 cut(s) 90, 195, 200, 590
FatI CATG 2 cut(s) 88, 588
FauI CCCGC 1 cut(s) 170
FokI GGATG 2 cut(s) 67, 219
GlaI GCGC 1 cut(s) 283
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 2 cut(s) 597, 621
HgaI GACGC 2 cut(s) 74, 549
HhaI GCGC 1 cut(s) 284
Hin1II CATG 2 cut(s) 92, 592
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HinfI GANTC 2 cut(s) 149, 624
Hpy166II GTNNAC 1 cut(s) 254
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 1 cut(s) 254
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 2 cut(s) 41, 217
HpyF3I CTNAG 1 cut(s) 75
Hsp92II CATG 2 cut(s) 92, 592
HspAI GCGC 1 cut(s) 282
Lsp1109I GCAGC 3 cut(s) 80, 170, 276
LweI GCATC 2 cut(s) 164, 197
MboII GAAGA 2 cut(s) 48, 158
MluCI AATT 3 cut(s) 5, 165, 606
MlyI GAGTC 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 468
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 71
MvaI CCWGG 8 cut(s) 109, 240, 258, 474, 513, 531, 552, 585
MvnI CGCG 1 cut(s) 543
NlaIII CATG 2 cut(s) 92, 592
NlaIV GGNNCC 1 cut(s) 510
NspI RCATGY 1 cut(s) 592
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 1 cut(s) 149
PflMI CCANNNNNTGG 5 cut(s) 296, 479, 518, 536, 557
PfoI TCCNGGA 1 cut(s) 415
PleI GAGTC 1 cut(s) 632
PpsI GAGTC 1 cut(s) 632
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 8 cut(s) 107, 238, 256, 472, 511, 529, 550, 583
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 8 cut(s) 107, 238, 256, 472, 511, 529, 550, 583
PspN4I GGNNCC 1 cut(s) 510
PspPI GGNCC 1 cut(s) 236
PspPPI RGGWCCY 1 cut(s) 236
PstNI CAGNNNCTG 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 222
Sau96I GGNCC 1 cut(s) 236
SchI GAGTC 1 cut(s) 633
SetI ASST 4 cut(s) 241, 259, 277, 478
SfaNI GCATC 2 cut(s) 164, 197
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 222
SphI GCATGC 1 cut(s) 592
Sse9I AATT 3 cut(s) 5, 165, 606
TaaI ACNGT 1 cut(s) 221
TaqI TCGA 2 cut(s) 134, 162
TasI AATT 3 cut(s) 5, 165, 606
TauI GCSGC 9 cut(s) 74, 306, 390, 426, 525, 579, 615, 618, 621
TfiI GAWTC 1 cut(s) 149
TscAI CASTG 2 cut(s) 224, 250
TseI GCWGC 3 cut(s) 68, 183, 264
TspDTI ATGAA 2 cut(s) 7, 17
TspRI CASTG 2 cut(s) 224, 250
Van91I CCANNNNNTGG 5 cut(s) 296, 479, 518, 536, 557
VpaK11BI GGWCC 1 cut(s) 236
XapI RAATTY 1 cut(s) 5
XceI RCATGY 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.